TY - JOUR A1 - Kneipp, Janina A1 - Kneipp, H. A1 - Wittig, B. A1 - Kneipp, K. T1 - Following the dynamics of pH in endosomes of live cells with SERS nanosensors JF - The journal of physical chemistry / C N2 - The surface enhanced Raman scattering (SERS) spectrum of a reporter molecule attached to gold or silver nanostructures, which is pH-sensitive, can deliver information on the local pH in the environment of the nanostructure. Here, we demonstrate the use of a mobile SERS nanosensor made from gold nanaoaggregates and 4-mercaptobenzoic acid (pMBA) attached as a reporter for monitoring changes in local pH of the cellular compartments of living NIH/3T3 cells. We show that SERS nanosensors enable the dynamics of local pH in individual live cells to be followed at subendosomal resolution in a timeline of cellular processes. This information is of basic interest for a better understanding of a broad range of physiological and metabolic processes as well as for a number of biotechnological applications. PY - 2010 DO - https://doi.org/10.1021/jp910034z SN - 1932-7447 SN - 1089-5639 VL - 114 IS - 16 SP - 7421 EP - 7426 PB - Soc. CY - Washington, DC AN - OPUS4-23214 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -