TY - GEN A1 - Kage, Daniel T1 - FLiMFlow – Recent achievements in flow cytometry with lifetime detection and lifetime-encoded beads N2 - Flow cytometry is a widespread technique in biological research and clinical applications. Two different directions are currently of importance in development of new methods in this field. Whereas analyses in research become increasingly complex and require a growing number of simultaneously detectable codes and fluorescent labels, also low-cost methods and portable devices are needed in routine application. Lifetime encoding could present an alternative to common spectral multiplexing. On the one hand, it addresses the need for additional codes by combining spectral and lifetime multiplexing. On the other hand, using only lifetime encoding could help to reduce instrument costs by keeping the number of excitation sources and detectors low. Here, we report on our recent progress in employing dye-stained lifetime-encoded polymer microparticles as a model system for lifetime encoding in flow cytometry. The discrimination of two lifetime codes was achieved with two bead sets. Moreover, the simultaneous detection of a spectrally different ligand fluorescence signal could be demonstrated. T2 - FLiMFlow meeting CY - Pisa, Italy DA - 28.09.2016 KW - Flow cytometry KW - Fluorescence KW - Life sciences KW - Lifetime encoding KW - Polymer particles PY - 2016 UR - https://opus4.kobv.de/opus4-bam/frontdoor/index/index/docId/38239 AN - OPUS4-38239 AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany