TY - JOUR A1 - Silva, C.P. A1 - Lima, D.L.D. A1 - Schneider, Rudolf A1 - Otero, M. A1 - Esteves, V.I. T1 - Evaluation of the anthropogenic input of caffeine in surface waters of the north and center of Portugal by ELISA JF - The science of the total environment N2 - This study comprises the development of an enzyme-linked immunosorbent assay (ELISA) for the quantification of caffeine in complex aqueous matrices without any sample clean-up procedure. Salinity and dissolved organic matter were selected as potential interfering agents. The addition of a sample buffer containing bovine serum albumin (BSA) prior to the sample was found to decrease the influence of those interfering agents. The working range of the developed method was 0.1–100 µg L- 1. Quantification of caffeine was possible in 43 out of 51 real aqueous samples, at values between < LOD and 15 µg L- 1. Results correlate well with those obtained by LC–MS/MS. To the best of our knowledge this is the first study dealing with the quantification of caffeine in Portugal's surface waters. KW - Caffeine KW - Surface water KW - ELISA KW - Anthropogenic markers KW - Koffein KW - Eintrag KW - Oberflächenwasser KW - Gewässer KW - Abwasser KW - Antikörper KW - Immunoassay PY - 2014 DO - https://doi.org/10.1016/j.scitotenv.2014.01.120 SN - 0048-9697 VL - 479-480 SP - 227 EP - 232 PB - Elsevier CY - Amsterdam AN - OPUS4-30450 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -