TY - JOUR A1 - Lima, D.L.D. A1 - Schneider, Rudolf A1 - Esteves, V.I. T1 - Development of an enzyme-linked immunosorbent assay for atrazine monitoring in water samples JF - Environmental science and pollution research international N2 - The implementation of the Water Framework Directive (2000/60/EC) requires the establishment of monitoring programs. However, conventional procedures for sample preparation prior to chromatographic analysis are rather expensive and time consuming, being the development of cost-effective and easy tool a necessity. The aim of this work was to develop an enzyme-linked immunosorbent assay (ELISA) able to determine atrazine in water samples. Matrix effects evaluation showed that the increase of humic acid (HA) concentration leads to flattened calibration curves and to the loss of the sigmoidal shape. However, such interference was overcome, by the presence of an environmental sample buffer, incubated together with the samples. Recoveries from 88.5 to 119.2 % were obtained in the presence of HA concentrations up to 20 mg-1. An analytical range from 0.003 to 1 µgL-1 was obtained, and atrazine was detected in a sewage treatment plant with concentrations ranging from 14 to 52 ngL-1. KW - Immunoassay KW - Atrazine KW - Surface waters KW - Wastewater treatment plants KW - Matrix effects PY - 2013 DO - https://doi.org/10.1007/s11356-012-1227-z SN - 0944-1344 SN - 1614-7499 VL - 20 IS - 5 SP - 3157 EP - 3164 PB - Ecomed Publ. CY - Landsberg AN - OPUS4-28299 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -