TY - JOUR A1 - Montero-Calasanz, M.d.C. A1 - Göker, M. A1 - Pötter, G. A1 - Rohde, M. A1 - Spröer, C. A1 - Schumann, P. A1 - Gorbushina, Anna A1 - Klenk, H.-P. T1 - Geodermatophilus africanus sp. nov., a halotolerant actinomycete isolated from Saharan desert sand T2 - Antonie van Leeuwenhoek N2 - A novel Gram-strain positive, aerobic, actinobacterial strain, designated CF11/1T, was isolated from a sand sample obtained in the Sahara Desert, Chad. The black-pigmented isolate was aerobic and exhibited optimal growth from 25 to 35 °C at pH 6.0–8.0 and with 0–8 % (w/v) NaCl, indicating that it is a halotolerant mesophile. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the genus Geodermatophilus. The G+C content in the genome was 74.4 mol%. The peptidoglycan contained meso-diaminopimelic acid as diagnostic diaminoacid. The main phospholipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol and a minor fraction of phosphatidylglycerol; MK-9(H4) was the dominant menaquinone, and galactose was detected as a diagnostic sugar. The major cellular fatty acid was branched-chain saturated acid iso-C16:0. Analysis of 16S rRNA gene sequences showed 95.3–98.6 % pairwise sequence identity with the members of the genus Geodermatophilus. Based on phenotypic and chemotaxonomic properties, as well as phylogenetic distinctiveness, the isolate represents a novel species, Geodermatophilus africanus, with the type strain CF11/1T (DSM 45422 = CCUG 62969 = MTCC 11556). PB - Springer CY - Dordrecht KW - Actinomycetes KW - Geodermatophilaceae KW - Taxonomy KW - Osmotolerant KW - Phenotype microarray PY - 2013 UR - https://opus4.kobv.de/opus4-bam/frontdoor/index/index/docId/28868 AN - OPUS4-28868 SN - 0003-6072 SN - 1572-9699 VL - 104 IS - 2 SP - 207 EP - 216 AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany