TY - JOUR A1 - Sloniec, Jagoda A1 - Resch-Genger, Ute A1 - Hennig, Andreas T1 - Photophysics and release kinetics of enzyme-activatable optical probes based on H-dimerized fluorophores on self-immolative linkers JF - The journal of physical chemistry / B N2 - A series of activatable optical probes for the model enzyme penicillin G amidase based on intramolecular formation of non-fluorescent H-dimer between two identical dyes were synthesized. The probes are based on a self-immolative linker, which allows positioning both dyes in close spatial proximity to ensure efficient quenching of probes with absorption and fluorescence emission in the near-infrared (NIR) range. A detailed photophysical investigation of the novel optical probes led to a revision of a previously anticipated quenching mechanism and revealed their potential for optimizing the performance of activatable probes based on H-dimer formation. A kinetic analysis indicated that the fluorescence progress curves can be used to qualitatively extract enzyme kinetic parameters. PY - 2013 DO - https://doi.org/10.1021/jp409388b SN - 1520-6106 SN - 1520-5207 SN - 1089-5647 VL - 117 IS - 46 SP - 14336 EP - 14344 PB - Soc. CY - Washington, DC AN - OPUS4-30053 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -