TY - JOUR A1 - Dsouza, R.N. A1 - Hennig, Andreas A1 - Nau, W.M. T1 - Supramolecular tandem enzyme assays JF - Chemistry - A European journal N2 - We conceptualize a novel approach towards enzyme assays based on the reversible and competitive binding of a fluorescent dye and the substrate as well as product of an enzymatic reaction to a macrocyclic host. This method was termed 'supramolecular tandem assay', and has been applied to inhibitor and activator screening, sensor array development, and enantiomeric excess determination of amino acids. The simple and rapid read-out by fluorescence allows their straightforward implementation into high-throughput screening. KW - Enzyme assays KW - Fluorescent dyes KW - Macrocycles KW - Molecular recognition KW - Supramolecular chemistry PY - 2012 DO - https://doi.org/10.1002/chem.201103364 SN - 0947-6539 SN - 1521-3765 VL - 18 IS - 12 SP - 3444 EP - 3459 PB - Wiley-VCH Verl. CY - Weinheim AN - OPUS4-25644 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -