TY - CONF A1 - Koegler, M. A1 - Paul, Andrea A1 - Alborch, K. P. A1 - Birkholz, M. A1 - Junne, S. A1 - Neubauer, P. T1 - Raman on-line monitoring approach for bioprocess and bio-pharmaceutical manufacturing T2 - Tagungsband 12. Kolloquium Prozessanalytik N2 - There is a high demand of monitoring in the era of QbD in industrial scale require new approaches to gain data rapidly and of sufficient quality in real time. Raman spectroscopy technology has great potential but not yet shown it fully in process on-line monitoring due to limitations such as i) uncomplete separation between cells and growth media alone, ii) general weak Raman signals of analytes in complex solutions and iii) strong background signals such as the auto-fluorescence, cosmic rays and surrounding lights overlapping the weak Raman signals. Here we demonstrate a Proof-of-Concept on an the example lactic acid bacteria process using a Streptococcus thermophiles fermentation. Results from three different Raman approaches are presented: 1) Time-Gated Raman Spectroscopy (TG-Raman), 2) Surface Enhanced Raman Spectroscopy (SERS) and 3) Raman process spectroscopy with NIR excitation combined with multivariate data analysis (MVDA) using Principal Component Analysis (PCA) and Partial Least Squares Regression (PLSR). T2 - 12. Kolloquium Arbeitskreis Prozessanalytik CY - Berlin, Germany DA - 28.11.2016 KW - Raman KW - Chemometrics KW - SERS KW - TimeGate PY - 2016 SP - 91 EP - 93 CY - Berlin AN - OPUS4-38673 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -