TY - JOUR A1 - Müller, Larissa A1 - Jakubowski, Norbert A1 - Hayen, H. A1 - Roos, P.H. T1 - Iodination of proteins, proteomes and antibodies with potassium triodide for LA-ICP-MS based proteomic analyses T2 - Journal of analytical atomic spectrometry N2 - A fast and mild method for iodine labelling of proteins is presented which is specifically designed and optimized for laser ablation (LA-)ICP-MS based proteomics. Single proteins (lysozyme, bovine serum albumin, cytochrome c and β-casein), whole proteomes (microsomal proteome of rats) and antibodies (anti-bovine casein, anti-bovine serum albumin) can be efficiently iodinated by means of potassium triiodide with minimal losses of antigen properties and antibody binding to iodinated proteins. A comparison with iodination by use of IODO-Beads is presented and it is shown that triiodide labelling is a fast, cheap and less laborious alternative without compromising the analytical figures of merit. PB - Royal Society of Chemistry CY - London PY - 2011 UR - https://opus4.kobv.de/opus4-bam/frontdoor/index/index/docId/24209 AN - OPUS4-24209 SN - 0267-9477 SN - 1364-5544 N1 - Geburtsname von Müller, Larissa: Wäntig, L. - Birth name of Müller, Larissa: Wäntig, L. VL - 26 SP - 1610 EP - 1618 AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany