TY - JOUR A1 - Montero-Calasanz, M.d.C. A1 - Göker, M. A1 - Pötter, G. A1 - Rohde, M. A1 - Spröer, C. A1 - Schumann, P. A1 - Klenk, H.-P. A1 - Gorbushina, Anna T1 - Geodermatophilus telluris sp. nov., an actinomycete isolated from Saharan desert sand JF - International journal of systematic and evolutionary microbiology : IJSEM N2 - A novel Gram-positive, multiloculated thalli-forming, aerobic, actinobacterial strain, CF9/1/1T, was isolated in 2007 during environmental screening for xerophilic fungi in arid desert soil from the Sahara desert, Chad. The isolate grew best at a temperature range of 20–35 °C and at pH 6.0–8.5 and with 0–4% (w/v) NaCl, forming black-coloured and irregular colonies on GYM agar. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the genus Geodermatophilus. The DNA G+C content of the novel strain was 75.4 mol%. The peptidoglycan contained meso-diaminopimelic acid as a diagnostic diamino acid. The main phospholipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, a not yet structurally identified aminophospholipid and a small amount of phosphatidylglycerol; MK-9(H4) was identified as the dominant menaquinone and galactose was a diagnostic sugar. The major cellular fatty acids were branched-chain saturated acids: iso-C16:0 and iso-C15:0. The 16S rRNA gene sequence of the isolate showed 94.6–97.0% sequence similarities with those of five members of the genus: Geodermatophilus ruber DSM 45317T (94.6%), Geodermatophilus obscurus DSM 43160T (94.8%), Geodermatophilus siccatus DSM 45419T (96.2%), Geodermatophilus nigrescens DSM 45408T (96.7%) and Geodermatophilus arenarius DSM 45418T (97.0%). Based on the evidence from this polyphasic taxonomic study, a novel species, Geodermatophilus telluris sp. nov., is proposed; the type strain is CF9/1/1T (=DSM 45421T=CCUG 62764T). PY - 2013 DO - https://doi.org/10.1099/ijs.0.046888-0 SN - 1466-5026 SN - 1466-5034 VL - 63 SP - 2254 EP - 2259 PB - SGM CY - Reading AN - OPUS4-28982 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -