TY - GEN A1 - Raysyan, Anna T1 - Development of a quick test to screen for bisphenol A release from polymer materials N2 - Bisphenol A (BPA) is a common industrial chemical that is widely used as a monomer or additive in the production of polymer materials. This substance causes impairment of the childbearing function, acceleration of sexual aging, negatively impacts the brain, and also contributes to the development of certain oncological diseases, as well as increasing the risk of a fetus developing the Down syndrome. But the biggest downside of bisphenol is the fact that it accumulates in the system during its use, as well as after. Many methods have been proposed for the determination of BPA, in particular, gas and liquid chromatography. The main disadvantages of such approaches are the high cost of the equipment, the significant duration of an analysis, and difficulties in screening a large number of samples. Immunoanalytical techniques attributes are high throughput, high specificity and sensitivity, as well as low cost and simplicity. Particularly interesting as a quick test for screenings is the lateral flow immunoassay (LFIA), a format widely in use for the detection of pregnancy. LFIA for BPA have been described before, but lack sensitivity. In this work, focuses on the conjugation (by passive adsorption) efficiency of the different monoclonal antibodies with 40 nm gold nanoparticles. The effect of factors such as pH value and concentration of antibody has been quantificationally discussed using spectra methods after adding 10% NaCl which induced gold nanoparticle aggregation. Consequently, 40 nm gold nanoparticles should be labeled with antibody under optimal pH value and optimal concentrations of antibody. It will be helpful for the application of antibody-labeled gold nanoparticles for the determination of bisphenol A in aqueous solutions (water from different sources, leachates and extracts). T2 - Adlershofer Forschungsforum 2017 CY - Berlin, Germany DA - 10.11.2017 KW - Bisphenol A KW - BPA KW - LFIA KW - ELISA KW - GNP KW - Passive absorption PY - 2017 UR - https://opus4.kobv.de/opus4-bam/frontdoor/index/index/docId/43125 AN - OPUS4-43125 AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany