TY - JOUR A1 - Drescher, Daniela A1 - Giesen, Charlotte A1 - Traub, Heike A1 - Panne, Ulrich A1 - Kneipp, Janina A1 - Jakubowski, Norbert T1 - Quantitative imaging of gold and silver nanoparticles in single eukaryotic cells by laser ablation ICP-MS T2 - Analytical chemistry N2 - Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) was utilized for spatially resolved bioimaging of the distribution of silver and gold nanoparticles in individual fibroblast cells upon different incubation experiments. High spatial resolution was achieved by optimization of scan speed, ablation frequency, and laser energy. Nanoparticles are visualized with respect to cellular substructures and are found to accumulate in the perinuclear region with increasing incubation time. On the basis of matrix-matched calibration, we developed a method for quantification of the number of metal nanoparticles at the single-cell level. The results provide insight into nanoparticle/cell interactions and have implications for the development of analytical methods in tissue diagnostics and therapeutics. PB - American Chemical Society CY - Washington, DC KW - Imaging KW - Cell KW - Nanoparticles KW - Laser ablation KW - ICP-MS PY - 2012 UR - https://opus4.kobv.de/opus4-bam/frontdoor/index/index/docId/27440 AN - OPUS4-27440 SN - 0003-2700 SN - 1520-6882 VL - 84 IS - 22 SP - 9684 EP - 9688 AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany