TY - JOUR A1 - Schulte, Franziska A1 - Mäder, J. A1 - Kroh, L.W. A1 - Panne, Ulrich A1 - Kneipp, Janina T1 - Characterization of pollen carotenoids with in situ and high-performance thin-layer chromatography supported resonant Raman spectroscopy T2 - Analytical chemistry N2 - Raman signatures of the carotenoid component are studied in individual pollen grains from different species of trees. The information is obtained as differences in the strong pre-resonant Raman spectra measured before and after photodepletion of the carotenoid molecules. The results provide the first in situ evidence of interspecies differences in pollen carotenoid content, structure, and/or assembly between plant species without prior purification. The analysis of carotenoids in situ is confirmed by high-performance thin-layer chromatography (HPTLC)-supported resonance Raman data measured directly on the HPTLC plates after separation of carotenoids in pollen extracts. Utilization of the in situ, extraction-free procedure in carotenoid analysis will improve sensitivity and structural selectivity and provides insight into carotenoid structure and composition in single pollen grains. PB - American Chemical Society CY - Washington, DC PY - 2009 UR - https://opus4.kobv.de/opus4-bam/frontdoor/index/index/docId/20260 AN - OPUS4-20260 SN - 0003-2700 SN - 1520-6882 VL - 81 IS - 20 SP - 8426 EP - 8433 AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany