TY - JOUR A1 - VeĢliz Montes, Cinthya A1 - Memczak, H. A1 - Gyssels, E. A1 - Torres, T. A1 - Madder, A. A1 - Schneider, Rudolf T1 - Photoinduced cross-linking of short Furan-modified DNA on surfaces JF - Langmuir N2 - We report for the first time the formation of sitespecific interstrand cross-linked (ICL) surface-immobilized furanmodified DNA duplexes via singlet oxygen. 1O2, necessary for effecting furan-mediated ICL formation, was produced in situ using methylene blue or a zinc phthalocyanine derivative (TT1) as a photosensitizer. Via surface plasmon resonance spectroscopy, we show that surface ICL was achieved, and a robust link formed that enhances the stability of the 12-mer duplex even after surface regeneration. The described method represents a novel platform technology based on surfaces with addressable and stable DNA duplexes requiring only short oligonucleotides. KW - DNA interstrand cross-link (ICL) KW - Furanmodified DNA duplexes KW - Surface plasmon resonance spectroscopy PY - 2017 DO - https://doi.org/10.1021/acs.langmuir.6b03855 SN - 0743-7463 SN - 1520-5827 VL - 33 IS - 5 SP - 1197 EP - 1201 PB - ACS Publications AN - OPUS4-39155 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -