TY - JOUR A1 - Drescher, Daniela A1 - Orts Gil, Guillermo A1 - Laube, G. A1 - Natte, Kishore A1 - Veh, R.W. A1 - Ă–sterle, Werner A1 - Kneipp, Janina T1 - Toxicity of amorphous silica nanoparticles on eukaryotic cell model is determined by particle agglomeration and serum protein adsorption effects T2 - Analytical and bioanalytical chemistry N2 - Cell cultures form the basis of most biological assays conducted to assess the cytotoxicity of nanomaterials. Since the molecular environment of nanoparticles exerts influence on their physicochemical properties, it can have an impact on nanotoxicity. Here, toxicity of silica nanoparticles upon delivery by fluid-phase uptake is studied in a 3T3 fibroblast cell line. Based on XTT viability assay, cytotoxicity is shown to be a function of (1) particle concentration and (2) of fetal calf serum (FCS) content in the cell culture medium. Application of dynamic light scattering shows that both parameters affect particle agglomeration. The DLS Experiments verify the stability of the nanoparticles in culture medium without FCS over a wide range of particle concentrations. The related toxicity can be mainly accounted for by single silica nanoparticles and small agglomerates. In contrast, agglomeration of silica nanoparticles in all FCS-containing media is observed, resulting in a decrease of the associated toxicity. This result has implications for the evaluation of the cytotoxic potential of silica nanoparticles and possibly also other nanomaterials in standard cell culture. PB - Springer CY - Berlin KW - Agglomeration KW - Cytotoxicity KW - Fibroblast cells KW - Serum proteins KW - Silica nanoparticles PY - 2011 UR - https://opus4.kobv.de/opus4-bam/frontdoor/index/index/docId/23678 AN - OPUS4-23678 SN - 1618-2642 SN - 1618-2650 VL - 400 IS - 5 SP - 1367 EP - 1373 AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany