TY - GEN A1 - Hahn, Marc Benjamin T1 - From sensing to dosimetry: Immobilized single-stranded DNA on surfaces as versatile sensor for protein activity and irradiation damage N2 - We report on the design, preparation and application of immobilized Oligonucleotides as biological sensors for dosimetry and protein activity. Oligonucleotides can be custom tailored in terms of sequence and length to the needs of the specific application. The preparation can be scaled from small lab-scales to higher output via spotting technology on various surfaces such as glass, silicon-dioxide or gold. They can be applied for the sensing of DNA-protein interaction and surface-plasmon resonance to detect the influence of cosolutes on the protein activity. This can be applied in the testing of pharmaceutical or cosmsetic applications and incorporated in microfluidic devices. Other important use cases are the usage in dosimetric application where the oligonucleotides are subjected to ionizing radiation. They can directly act as a sensor for biological damage which is detected and quantified by the subsequent hybridization of complementary strands who carry a fluorescence dye. The quantification can be performed by an array scanner or fluorescence microscope. Investigation on a molecular level can be performed with the AFM-IR technique. Applications are calibration of radiation sources and the investigation of interaction of ionizing radiation with biomolecules. T2 - Euromat 2017 CY - Thessaloniki, Greece DA - 17.09.2017 KW - DNA KW - Protein KW - Dosimetry KW - Radiation damage KW - SPR KW - Surface modification PY - 2017 UR - https://opus4.kobv.de/opus4-bam/frontdoor/index/index/docId/42619 AN - OPUS4-42619 AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany