TY - CONF A1 - Bell, Jérémy A1 - Ashokkumar, Pichandi A1 - Buurman, Merwe A1 - Rurack, Knut T1 - Sugar detection in sodas utilizing a fluorescent microfluidics sensor T2 - IMA 2015 N2 - Because of the globally increasing prevalence of diabetes, the need for accurate, efficient and at best miniaturized automated analytical systems for sugar detection is still urgent. The development of molecular probes for sugar based on boronic acid receptors1 offers an excellent alternative to the kinetically slow enzyme-based sugar sensors. Moreover, by coupling such chelating units with rhodamines, fluorescein or BODIPY moieties, colorimetric and/or highly fluorescent sugar sensing schemes can be obtained.2 In this work, a boronic acid-functionalized BODIPY probe is described; it binds selectivity to fructose's adjacent diols to form cyclic boronate esters in partially aqueous solutions, with a broad pH range compatibility and a sensitivity in the micromolar range. To enhance the applicability of the fluorometric test in the sense described above, integration with a microfluidic sensor was achieved. The miniaturization of chemical analysis systems yields many functional and economical benefits such as low cost, ease of use, high stability and good portability. With similar selectivity and sensitivity, fructose was detected by fluorescence in real time in the chip, and an assay for the straightforward detection of sugar in sodas was achieved. T2 - IMA 2015 CY - Kalamata, Grece DA - 2015-09-20 PY - 2015 AN - OPUS4-34776 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -