TY - CONF A1 - Climent Terol, Estela T1 - Design and synthesis of lateral flow tests containing antibody-gated materials N2 - Methods for the rapid and sensitive detection of target analytes are gaining importance in medical diagnostics and environmental monitoring, in the security, occupational health and safety as well as food sectors. Among all the methods employed for rapid tests, lateral flow assays (LFAs) are the most commonly used. However, some drawbacks are that most of these tests either indicate the analyte only indirectly, and in other cases the sensitivity is not high enough. Keeping in mind these limitations, the use of stimuli-responsive materials for small-molecule sensing relying on chemical signal amplification and utilizing specific interactions between biomolecules such as antibodies and the corresponding analytes are particularly attractive. Such materials can also be incorporated on paper strips for lateral-flow assays in a straightforward manner. These sensing materials comprise mesoporous silica nanoparticles loaded with indicator molecules and containing certain hapten derivative molecules covalently grafted at the surface, which bind to the respective antibody and inhibit the release of a dye as reporter (Scheme 1). In presence of the designated analyte, a displacement of the antibody from the material is observed, allowing the release of dye. Because a large number of indicator molecules can be released when a single analyte molecule binds to an antibody cap, a strong signal amplification is observed. Considering the modularity, high sensitivity and selectivity of these antibody-gated indicator delivery systems, the presentation discusses general aspects of system design as well as analytical performance and highlights the integration into a lateral-flow assay, showing as an example the determination of the explosives TATP, TNT and PETN with a fluorescence readout, in single-substance and multiplexing modes. T2 - International Webinar on Biosensors and Bioelectronics CY - Online meeting DA - 20.07.2020 KW - Multiplexing detection KW - Rapid testing methods KW - Gated hybrid materials KW - Lateral flow tests KW - Explosives detection PY - 2020 UR - https://europeanmeetings.net/conferences/biosensor-bioelectronics AN - OPUS4-51038 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -