TY - CONF A1 - Resch-Genger, Ute A1 - Pauli, Jutta T1 - Characterization of dye-protein conjugates with optical methods N2 - Fluorophore labeled proteins and antibodies, referred to also as targeted optical probes, present a promising strategy for a variety of applications from fundamental cell-based biological studies to in vivo diagnostics and image guided surgeries in humans. In this respect, design strategies for the preparation of such conjugates from different dyes including analyte-responsive fluorophores are presented as well as their analytical and spectroscopic characterization employing Absorption spectroscopy and steady state and time-resolved fluorometry. Special emphasis is dedicated to the influence of dye hydrophilicity and labeling density on the optical properties and binding behavior of these dye-bioconjugates including their performance in in vitro and in vivo bioimaging studies. T2 - International Workshop “Affinity - Mass spectrometry – New Methods and Application to Protein Therapeutics Development” CY - Rüsselsheim am Main, Germany DA - 14.11.2016 KW - Fluorescence KW - Dye bioconjugates KW - Protein KW - Immunoassay KW - Quality assurance KW - Optical characterization KW - Dye KW - NIR KW - Imaging PY - 2016 AN - OPUS4-38504 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -