TY - CONF A1 - Nirmalananthan-Budau, Nithiya A1 - Moser, Marko A1 - Behnke, Thomas A1 - Geißler, Daniel A1 - Resch-Genger, Ute T1 - Multimodal cleavable reporters for the optical quantification of functional groups on nano- and microparticles N2 - Polymer nanoparticles (NPs) are of increasing importance for a wide range of applications in the material and life sciences. This includes their application as carriers for e.g., analyte-responsive ligands for DNA sequencing platforms, drugs as well as dye molecules for use as multichromophoric reporters for signal enhancement in optical assays or the fabrication of nanosensors and targeted probes in bioimaging studies. All these applications require surface functionalization of the particles with e.g., ligands, sensor dyes, or analyte recognition moieties like biomolecules, and subsequently, the knowledge of the chemical nature and total number of surface groups as well as the number of groups accessible for coupling reactions. Particularly attractive for the latter are optically active reporters together with sensitive and fast optical assays, which can be read out with simple, inexpensive instrumentation. We assessed a variety of conventional and newly developed colorimetric and fluorometric labels for optical surface group analysis, utilizing e.g., changes in intensity and/or color for signal generation. Moreover, novel cleavable and multimodal reporters were developed which consist of a reactive group, a cleavable linker, and an optically active moiety, chosen to contain also heteroatoms for straightforward method validation by elemental analysis, ICP-OES, ICP-MS or NMR. In contrast to conventional labels measured bound at the particle surface, which can favor signal distortions by scattering and encoding dyes, cleavable reporters can be detected colorimetrically or fluorometrically both attached at the particle surface and after quantitative cleavage of the linker in the transparent supernatant after particle removal e.g., by centrifugation. Here, we present first results obtained for the optical quantification of carboxylic and amino groups on a series of self-made polystyrene NPs with different types of labels and compare their potential and drawbacks for surface group analysis. T2 - NanoWorkshop 2018 CY - Berlin, Germany DA - 14.05.2018 KW - Optical assays KW - Cleavable probes KW - Conventional dyes KW - Nanoparticles PY - 2018 N1 - Geburtsname von Nirmalananthan-Budau, Nithiya: Nirmalananthan, N. - Birth name of Nirmalananthan-Budau, Nithiya: Nirmalananthan, N. AN - OPUS4-45177 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -