TY - JOUR A1 - Meyer, S. A1 - López-Serrano, Ana A1 - Mitze, H. A1 - Jakubowski, Norbert A1 - Schwerdtle, T. T1 - Single-cell analysis by ICP-MS/MS as a fast tool for cellular bioavailability studies of arsenite T2 - Metallomics N2 - Single-cell inductively coupled plasma mass spectrometry (SC-ICP-MS) has become a powerful and fast tool to evaluate the elemental composition at a single-cell level. In this study, the cellular bioavailability of arsenite (incubation of 25 and 50 μM for 0-48 h) has been successfully assessed by SC-ICP-MS/MS for the first time directly after re-suspending the cells in water. This procedure avoids the normally arising cell membrane permeabilization caused by cell fixation methods (e.g. methanol fixation). The reliability and feasibility of this SC-ICP-MS/MS approach with a limit of detection of 0.35 fg per cell was validated by conventional bulk ICP-MS/MS analysis after cell digestion and parallel measurement of sulfur and phosphorus. PB - RSC Publ. CY - Cambridge KW - Single-cell analysis KW - ICP-MS/MS KW - Arsenite PY - 2018 UR - https://opus4.kobv.de/opus4-bam/frontdoor/index/index/docId/44110 AN - OPUS4-44110 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-441105 SN - 1756-5901 VL - 10 IS - 1 SP - 73 EP - 76 AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany