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Roundup® is the brand name for herbicide solutions containing glyphosate, which specifically inhibits the 5-enolpyruvyl-shikimate-3-phosphate (EPSP) synthase of the shikimate pathway. The inhibition of the EPSP synthase causes plant death because EPSP is required for biosynthesis of aromatic amino acids. Glyphosate also inhibits the growth of archaea, bacteria, Apicomplexa, algae and fungi possessing an EPSP synthase. Here, we have characterized two glyphosate-resistant bacteria from a Roundup solution. Taxonomic classification revealed that the isolates 1CH1 and 2CH1 are Burkholderia anthina and Burkholderia cenocepacia strains respectively. Both isolates cannot utilize glyphosate as a source of phosphorus and synthesize glyphosate-sensitive EPSP synthase variants. Burkholderia. anthina 1CH1 and B. cenocepacia 2CH1 tolerate high levels of glyphosate because the herbicide is not taken up by the bacteria. Previously, it has been observed that the exposure of soil bacteria to herbicides like glyphosate promotes the development of antibiotic resistances. Antibiotic sensitivity testing revealed that the only the B. cenocepacia 2CH1 isolate showed increased resistance to a variety of antibiotics. Thus, the adaptation of B. anthina 1CH1 and B. cenocepacia 2CH1 to glyphosate did not generally increase the antibiotic resistance of both bacteria. However, our study confirms the genomic adaptability of bacteria belonging to the genus Burkholderia.
In the following parts, a damage analysis of an extrusion line is presented. An important
point is the recording of the damage and the consideration of all facts around the operation and the history of the machine. The investigations in the laboratory confirmed the facts gathered in the hypothesis [1].
The main cause to be assumed is the age of the machine and the resulting load changes, in connection with the additional holes and the carbide concentration. By an unfavourable coincidence, a borehole was drilled in a place where there was a high concentration of carbides.
From the already partially corroded fracture edges, it can be assumed that it was a gradual process until the final fracture of the component occurred. The fracture of the component could be prevented in the future by a different maintenance strategy for machines of the same construction [2].
Belt conveyors are highly efficient systems used in mining and other industries to transport bulk materials, mainly brown coal and overburden, over distances ranging from short distances to several kilometres. For technical reasons, their operation is associated with noise emissions. During inspections and maintenance this can lead to increased noise exposure of employees. In the vicinity of residential buildings or in areas needing protection, exceeding the emission limits can lead to a temporary restriction of the operation of these high performance installations. Measures are required to reduce the noise impact primarily at the source or in the immediate vicinity. The acoustic and mechanical parameters are investigated on a test bench at the Technical University of Brandenburg (BTU) Cottbus-Senftenberg as part of the drive assembly tests in cooperation with LEAG. The stages of these tests are: Run-up test. In this test the supporting roller is accelerated under test load to a circumferential speed of 10 m/s and the sound pressure level is recorded. This makes it possible to calculate the sound emission as a function of the peripheral speed; Recording the geometry of the idler gear. An accurate description of the geometry of the idler housing allows conclusions to be drawn about the possible causes of excitation. The defined characteristics serve to quantify the acoustic effect of the driven rollers and derive specifications for use in belt conveyor systems. Based on the special requirements to the planned belt conveyor system, an adapted selection of low-noise idler rollers can be made at an early stage on the basis of specified parameters and confirmed test results.
Brevundimonas is a genus of freshwater bacteria belonging to the family Caulobacteraceae. The present study describes two novel species of the genus Brevundimonas (LVF1 T and LVF2 T ). Both were genomically, morphologically, and physiologically characterized. Average nucleotide identity analysis revealed both are unique among known Brevundimonas strains. In silico and additional ProphageSeq analyses resulted in two prophages in the LVF1 T genome and a remnant prophage in the LVF2 T genome. Bacterial LVF1 T cells form an elliptical morphotype, in average 1 µ m in length and 0.46 µ m in width, with a single flagellum. LVF2 T revealed motile cells approximately 1.6 µ m in length and 0.6 µ m in width with a single flagellum, and sessile cell types 1.3 µ m in length and 0.6 µ m in width. Both are Gram-negative, aerobic, have optimal growth at 30 ◦ C (up to 0.5 to 1% NaCl). Both are resistant towards erythromycin, meropenem, streptomycin, tetracycline and vancomycin. Anaerobic growth was observed after 14 days for LVF1 T only. For LVF1 T the name Brevundimonas pondensis sp. nov. and for LVF2 T the name Brevundimonas goettingensis sp. nov. are proposed. Type strains are LVF1 T (=DSM 112304 T = CCUG 74982 T = LMG 32096 T ) and LVF2 T
(=DSM 112305 T = CCUG 74983 T = LMG 32097 T ).