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Novel HepG2 cell clones 1A2 C2 and 1A2 C7 were independently generated by lentiviral transduction to functionally overexpress cytochrome P450 1A2 (CYP1A2). We found similar and stable CYP1A2 transcript and protein levels in both cell clones leading to specific enzyme activities of about 370 pmol paracetamol x min-1 x
mg-1 protein analyzed by phenacetin conversion. Both clones showed dramatically increased sensitivity to the hepatotoxic compound aflatoxin B1 (EC50<100 nM) when compared to parental HepG2 cells (EC50 ∼5 μM). Thus, newly established cell lines are an appropriate tool to study metabolism and toxicity of substances depending on conversion by CYP1A2.
In the German regional climate modeling project ReKliEs-De the existing EURO-CORDEX simulations have been systematically complemented by new simulations to derive more robust ranges of possible future climate change. The focus of the project lay on user tailored results, which are required for the planning of measures to adapt to the changing climate. Changes in temperature and precipitation indices are calculated from a multi model ensemble for the end of the 21st century. The results for the mitigation scenario RCP2.6 are compared to the results of the “business as usual” scenario RCP8.5. Averaged over Germany the increase of mean annual temperature and of the number of summer days will be around 3 times higher for RCP8.5 than for RCP2.6. In summer, the increase of dry days could be twice as high in RCP8.5 compared to RCP2.6.