• search hit 50 of 149
Back to Result List

Metaproteome analysis of sewage sludge from membrane bioreactors

  • Microbial dynamics and enzymatic activities of activated sludge processes are not completely understood yet. A better understanding about the biology is indispensable for further process optimization. Since proteins play a key role as catalysts in sludge processes, a protocol for protein extraction and analysis by 2-D PAGE was established. It is based on phenol extraction of alkaline extracts and on a subsequent precipitation with ammonium sulphate. 2-D protein patterns obtained from different sludges collected from membrane bioreactors showed – besides common spots – significant differences. Selected proteins were identified with nano- HPLC-ESI-MS/MS. All membrane biological reactor (MBR) sludge samples investigated in this study contained elastase 3A, which implies that this human serine protease is a significant constituent of municipal wastewater. Although the identification of proteins from ammonia-oxidizing bacterium Nitrosomonas europaea was expected, the detection of a protein with homology to the marine bacteriumMicrobial dynamics and enzymatic activities of activated sludge processes are not completely understood yet. A better understanding about the biology is indispensable for further process optimization. Since proteins play a key role as catalysts in sludge processes, a protocol for protein extraction and analysis by 2-D PAGE was established. It is based on phenol extraction of alkaline extracts and on a subsequent precipitation with ammonium sulphate. 2-D protein patterns obtained from different sludges collected from membrane bioreactors showed – besides common spots – significant differences. Selected proteins were identified with nano- HPLC-ESI-MS/MS. All membrane biological reactor (MBR) sludge samples investigated in this study contained elastase 3A, which implies that this human serine protease is a significant constituent of municipal wastewater. Although the identification of proteins from ammonia-oxidizing bacterium Nitrosomonas europaea was expected, the detection of a protein with homology to the marine bacterium Saprospira grandis in MBR1 was surprising.show moreshow less

Export metadata

Additional Services

Search Google Scholar
Metadaten
Author: Ramona KuhnORCiD, Dirk BenndorfORCiD, Erdmann Rapp, Udo Reichl, Luigi Leonardo Palese, Alfieri Pollice
DOI:https://doi.org/10.1002/pmic.201000590
ISSN:1615-9861
Title of the source (English):Proteomics
Document Type:Scientific journal article peer-reviewed
Language:English
Year of publication:2011
Contributing Corporation:IRSA
Tag:De novo sequencing; Membrane bioreactor; Microbiology; Protein extraction; metaproteomics; microbial communities
Volume/Year:11
Issue number:13
First Page:2738
Last Page:2744
Faculty/Chair:Fakultät 2 Umwelt und Naturwissenschaften / FG Biotechnologie der Wasseraufbereitung
Einverstanden ✔
Diese Webseite verwendet technisch erforderliche Session-Cookies. Durch die weitere Nutzung der Webseite stimmen Sie diesem zu. Unsere Datenschutzerklärung finden Sie hier.