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A spectrophotometric assay for the detection of fungal peroxygenases

  • Rapid and simple spectrophotometric methods are required for the unambiguous detection of recently discovered fungal peroxygenases in vivo and in vitro. This paper describes a peroxygenase-specific assay using 5-nitro-1,3-benzodioxole as substrate. The product, 4-nitrocatechol, produces a yellow color at pH 7, which can be followed over time at 425 nm (ε425 = 9,700 M−1 cm−1), and a red color when adjusted to pH >12, which can be measured in form of an end-point determination at 514 nm (ε514 = 11,400 M−1 cm−1). The assay is suitable for detecting peroxygenase activities in complex growth media and environmental samples as well as for high-throughput screenings.

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Author: Marzena Poraj-Kobielska, Matthias Kinne, René UllrichORCiD, Katrin Scheibner, Martin HofrichterORCiD
URL:http://www.sciencedirect.com/science/article/pii/S0003269711006610
URL:http://1096-0309
DOI:https://doi.org/10.1016/j.ab.2011.10.009
Title of the source (English):Analytical Biochemistry
Document Type:Scientific journal article peer-reviewed
Language:English
Year of publication:2012
Tag:5-Nitro-1,3-benzodioxole; Chloroperoxidase; Heme-thiolate; Monooxygenase; P450; Peroxidase
Volume/Year:421
Issue number:1
First Page:327
Last Page:329
Faculty/Chair:Fakultät 2 Umwelt und Naturwissenschaften / FG Enzymtechnologie
Institution name at the time of publication:Fakultät für Naturwissenschaften (eHL) / Prof. Enzymtechnologie
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