A spectrophotometric assay for the detection of fungal peroxygenases
- Rapid and simple spectrophotometric methods are required for the unambiguous detection of recently discovered fungal peroxygenases in vivo and in vitro. This paper describes a peroxygenase-specific assay using 5-nitro-1,3-benzodioxole as substrate. The product, 4-nitrocatechol, produces a yellow color at pH 7, which can be followed over time at 425 nm (ε425 = 9,700 M−1 cm−1), and a red color when adjusted to pH >12, which can be measured in form of an end-point determination at 514 nm (ε514 = 11,400 M−1 cm−1). The assay is suitable for detecting peroxygenase activities in complex growth media and environmental samples as well as for high-throughput screenings.
Author: | Marzena Poraj-Kobielska, Matthias Kinne, René UllrichORCiD, Katrin Scheibner, Martin HofrichterORCiD |
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URL: | http://www.sciencedirect.com/science/article/pii/S0003269711006610 |
URL: | http://1096-0309 |
DOI: | https://doi.org/10.1016/j.ab.2011.10.009 |
Title of the source (English): | Analytical Biochemistry |
Document Type: | Scientific journal article peer-reviewed |
Language: | English |
Year of publication: | 2012 |
Tag: | 5-Nitro-1,3-benzodioxole; Chloroperoxidase; Heme-thiolate; Monooxygenase; P450; Peroxidase |
Volume/Year: | 421 |
Issue number: | 1 |
First Page: | 327 |
Last Page: | 329 |
Faculty/Chair: | Fakultät 2 Umwelt und Naturwissenschaften / FG Enzymtechnologie |
Institution name at the time of publication: | Fakultät für Naturwissenschaften (eHL) / Prof. Enzymtechnologie |