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Determination of Sucrose in Honey with Derivatization/Solid-Phase-Microextraction and Gas-Chromatography/Mass Spectrometry.

  • A new method for the determination of sucrose in honey with derivatization solid-phase microextraction and gas chromatography/mass spectrometry (D-SPME–GC/MS) was developed. The method incorporates a sample derivatization with acetic anhydride using N-methylimidazole as the catalyst and the subsequent enrichment of the analyte in a Polyacrylate-SPME fiber. Results show that 100 µL N-methylimidazole and 800 µL acetic anhydride were sufficient to complete the acetylation for sucrose in 100 µL aqueous sample at room temperature. For SPME, an enrichment time of 30 min was sufficient. SPME was performed by immersing the fiber into the solution with additional vibration. Then, the analyte was desorbed for 5 min at 280°C in the GC/MS injection port with splitless mode. The present method exhibits good linearity at a concentration range of 0.3–8% of sucrose in honey with excellent regression (R = 0.9993). The method has been successfully applied to the control of sucrose adulteration in honey.

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Metadaten
Author: Haijing Wang, Helmut Geppert, Thomas FischerORCiD, Wolfgang Wieprecht, Detlef Möller
DOI:https://doi.org/10.1093/chromsci/bmv044
Title of the source (English):Journal of Chromatographic Science
Document Type:Scientific journal article peer-reviewed
Language:English
Year of publication:2015
Volume/Year:53
Issue number:9
First Page:1427
Last Page:1431
Faculty/Chair:Fakultät 2 Umwelt und Naturwissenschaften / FG Luftchemie und Luftreinhaltung
Zentrale Einrichtungen / Zentrale Analytik der BTU Cottbus-Senftenberg (ZA-BTU) / Zentrales Analytisches Labor
Institution name at the time of publication:Fakultät für Umweltwissenschaften und Verfahrenstechnik (eBTU) / LS Luftchemie und Luftreinhaltung
Fakultät für Umweltwissenschaften und Verfahrenstechnik (eBTU) / Zentrales Analytisches Labor
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