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Bacterial production and purification of immunoreactive paraneoplastic neurological syndrome autoantigen Ma2

  • Paraneoplastic neurological syndromes (PNS) are caused by an immune response against neuronal proteins upon their ectopical expression in tumor cells. Ma2 belongs to the protein family of paraneoplastic Ma antigens (PNMA). Detection of Ma2-specific autoantibodies is relevant for diagnostics of anti-Ma2 PNS and an underlying tumor such as germ testicular cancer, small cell lung cancer or breast cancer. Thus, early tumor treatment should improve the outcome for PNS therapy either. Dot blot immunoassay based on recombinantly expressed and purified autoantigens could offer a sensitive method for identification of paraneoplastic autoantibodies from sera of PNS patients. Here we present purification with IMAC and FPLC of human Ma2 autoantigen upon its recombinant expression in E.coli. Furthermore, we provide evidence that dot blot immunoassays with purified Ma2 autoantigen can be used for detection of Ma2-specific autoantibodies from sera of PNS patients.

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Author: Elisabeth Nowak, F. Martin, Sandra George, Nadja Röber, Karsten Conrad, Jan-Heiner KüpperGND
URL:https://content.iospress.com/articles/journal-of-cellular-biotechnology/jcb15029
DOI:https://doi.org/10.3233/JCB-15029
ISSN:2352-3697
Title of the source (English):Journal of Cellular Biotechnology
Document Type:Scientific journal article peer-reviewed
Language:English
Year of publication:2017
Tag:Autoimmune disease; FPLC; IMAC; Ma2; PNMA2; PNS; T7 expression system; autoantibodies; dot blot immunoassay
Volume/Year:2
Issue number:2
First Page:85
Last Page:91
Faculty/Chair:Fakultät 2 Umwelt und Naturwissenschaften / FG Molekulare Zellbiologie
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