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Exploring the role of surface modifications of TiNi-based alloys in evaluating in vitro cytocompatibility: a comparative study

  • The aim of this study was the comparative analysis of in vitro bio-testing of solid and porous TiNi samples with modified surfaces (intact, oxidated, and etched). Tests for cytocompatibility, hemolysis, and cytotoxicity (MTT) as well as visualization by confocal and scanning electron microscopy have shown that the chemically modified samples are the most cytocompatible. The intact and etched samples did not induce hemolysis greater than 2%, and thus they comply with the ISO 10993-4:2018 standard for hemolysis by blood-contacting biomaterials. Direct culture of etched samples with MCF-7 cells and human leukocytes showed low cytotoxicity. At the same time, the cytotoxicity of samples oxidated at 500 °C was significantly greater than that of the etched samples. Confocal and electron microscopy also confirmed the abovementioned quantitative data. The cells attached to the etched surface in numbers sufficient for them to be able to grow and proliferate on this substrate in vitro. These findings indicate that solid and porous TiNi alloyThe aim of this study was the comparative analysis of in vitro bio-testing of solid and porous TiNi samples with modified surfaces (intact, oxidated, and etched). Tests for cytocompatibility, hemolysis, and cytotoxicity (MTT) as well as visualization by confocal and scanning electron microscopy have shown that the chemically modified samples are the most cytocompatible. The intact and etched samples did not induce hemolysis greater than 2%, and thus they comply with the ISO 10993-4:2018 standard for hemolysis by blood-contacting biomaterials. Direct culture of etched samples with MCF-7 cells and human leukocytes showed low cytotoxicity. At the same time, the cytotoxicity of samples oxidated at 500 °C was significantly greater than that of the etched samples. Confocal and electron microscopy also confirmed the abovementioned quantitative data. The cells attached to the etched surface in numbers sufficient for them to be able to grow and proliferate on this substrate in vitro. These findings indicate that solid and porous TiNi alloy with surface modifications achieved by a cost-effective method is biotolerable and promising for clinical use and for tissue engineering.show moreshow less

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Author: Oleg Kokorev, Timofey ChekalkinORCiD, Ekaterina Marchenko, Yuri Yasenchuk, Sergey Gunther, Vladimir Serebrov, Alena Chernyshova, Aleksei ObrosovORCiD, Ji-hoon Kang
URL:https://iopscience.iop.org/article/10.1088/2051-672X/abc0f9/meta
DOI:https://doi.org/10.1088/2051-672X/abc0f9
ISSN:2051-672X
Title of the source (English):Surface Topography: Metrology and Properties
Document Type:Scientific journal article peer-reviewed
Language:English
Year of publication:2020
Tag:TiNi alloys; biocompatibility; cytocompatibility; surface modification; tissue engineering
Volume/Year:8
Issue number:4
Number of pages:14
Article number:045015
Faculty/Chair:Fakultät 3 Maschinenbau, Elektro- und Energiesysteme / FG Metallkunde und Werkstofftechnik
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