TY - GEN A1 - Mohsin, Mashkoor A1 - Raza, Shahbaz A1 - Roschanski, Nicole A1 - Guenther, Sebastian A1 - Ali, Aamir A1 - Schierack, Peter T1 - Description of the First Escherichia coli Clinical Isolate Harboring the Colistin Resistance Gene mcr-1 from the Indian Subcontinent T2 - Antimicrobial Agents and Chemotherapy Y1 - 2016 U6 - https://doi.org/10.1128/AAC.01945-16 SN - 1098-6596 SN - 0066-4804 VL - 61 IS - 1 ER - TY - GEN A1 - Sowa, Mandy A1 - Hiemann, Rico A1 - Schierack, Peter A1 - Reinhold, Dirk A1 - Conrad, Karsten A1 - Roggenbuck, Dirk T1 - Next-Generation Autoantibody Testing by Combination of Screening and Confirmation-the CytoBead® Technology T2 - Clinical Reviews in Allergy & Immunology Y1 - 2017 U6 - https://doi.org/10.1007/s12016-016-8574-3 SN - 1559-0267 SN - 1080-0549 VL - 53 IS - 1 SP - 87 EP - 104 ER - TY - GEN A1 - Kolenda, Rafał A1 - Burdukiewicz, Michał A1 - Schierack, Peter T1 - A systematic review and meta-analysis of the epidemiology of pathogenic Escherichia coli of calves and the role of calves as reservoirs for human pathogenic E. coli T2 - Frontiers in Cellular and Infection Microbiology Y1 - 2015 U6 - https://doi.org/10.3389/fcimb.2015.00023 SN - 2235-2988 VL - 5 ER - TY - GEN A1 - Rödiger, Stefan A1 - Kramer, Toni A1 - Frömmel, Ulrike A1 - Weinreich, Jörg A1 - Roggenbuck, Dirk A1 - Guenther, Sebastian A1 - Schaufler, Katharina A1 - Schröder, Christian A1 - Schierack, Peter T1 - Intestinal Escherichia coli colonization in a mallard duck population over four consecutive winter season T2 - Environmental Microbiology Y1 - 2015 U6 - https://doi.org/10.1111/1462-2920.12807 SN - 1462-2920 SN - 1462-2912 VL - 17 IS - 9 SP - 3352 EP - 3361 ER - TY - GEN A1 - Murugaiyan, Jayaseelan A1 - Krüger, Karolin A1 - Rösler, Uwe A1 - Weinreich, Jörg A1 - Schierack, Peter T1 - Assessment of species and antimicrobial resistance among Enterobacteriaceae isolated from mallard duck faeces T2 - Environmental Monitoring and Assessment Y1 - 2015 U6 - https://doi.org/10.1007/s10661-015-4346-4 SN - 0167-6369 SN - 1573-2959 VL - 187 IS - 3 SP - 127 EP - 137 ER - TY - GEN A1 - Reddig, Annika A1 - Lorenz, Sebastian A1 - Hiemann, Rico A1 - Guttek, Karina A1 - Hartig, Roland A1 - Heiserich, Lisa A1 - Peter, Vanessa A1 - Schierack, Peter A1 - Sack, Ulrich A1 - Roggenbuck, Dirk A1 - Reinhold, Dirk A1 - Eberle, Caroline T1 - Cytostatic drug resistance monitored by γH2AX T2 - Cytometry Part A Y1 - 2015 U6 - https://doi.org/10.1002/cyto.a.22667 SN - 1552-4930 SN - 1552-4922 VL - 87 IS - 8 SP - 724 EP - 732 ER - TY - GEN A1 - Lodemann, Ulrike A1 - Strahlendorf, Julia A1 - Schierack, Peter A1 - Klingspor, Shanti A1 - Aschenbach, Jörg R. A1 - Martens, Holger T1 - Effects of the Probiotic Enterococcus faecium and Pathogenic Escherichia coli Strains in a Pig and Human Epithelial Intestinal Cell Model T2 - Scientifica Y1 - 2015 U6 - https://doi.org/10.1155/2015/235184 VL - 2015 IS - 235184 ER - TY - GEN A1 - Villalta, Danilo A1 - Mytilinaiou, Maria G. A1 - Elsner, Martina A1 - Hentschel, Christian A1 - Cuccato, Juliane A1 - Somma, Valentina A1 - Schierack, Peter A1 - Roggenbuck, Dirk A1 - Bogdanos, Dimitrios Petrou T1 - Autoantibodies to asialoglycoprotein receptor (ASGPR) in patients with autoimmune liver diseases T2 - Clinica Chimica Acta Y1 - 2015 U6 - https://doi.org/10.1016/j.cca.2015.07.021 SN - 0009-8981 VL - 450 SP - 5 ER - TY - GEN A1 - Kolenda, Rafał A1 - Schierack, Peter A1 - Zieba, Filip A1 - Zwijacz-Kozica, Tomasz A1 - Bednarski, Michal T1 - First molecular characterization of Sarcocystis tenella in Tatra chamois (Rupicapra rupicapra tatrica) in Poland T2 - Parasitology Research Y1 - 2015 U6 - https://doi.org/10.1007/s00436-015-4619-4 SN - 0932-0113 SN - 1432-1955 VL - 114 IS - 10 SP - 3885 EP - 3892 ER - TY - GEN A1 - Rödiger, Stefan A1 - Burdukiewicz, Michał A1 - Blagodatskikh, Konstantin A. A1 - Jahn, Michael A1 - Schierack, Peter T1 - R as an Environment for the Reproducible Analysis of DNA Amplification Experiments T2 - The R journal Y1 - 2015 UR - https://journal.r-project.org/archive/2015/RJ-2015-011/index.html SN - 2073-4859 VL - 7 IS - 1 SP - 127 EP - 150 ER - TY - GEN A1 - Rödiger, Stefan A1 - Burdukiewicz, Michał A1 - Schierack, Peter T1 - chipPCR: an R package to pre-process raw data of amplification curves. Bioinformatics T2 - Bioinformatics Y1 - 2015 U6 - https://doi.org/10.1093/bioinformatics/btv205 SN - 1367-4803 SN - 1460-2059 VL - 31 IS - 17 SP - 2900 EP - 2902 ER - TY - CHAP A1 - Rödiger, Stefan A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Stavitskaya, Luba A1 - Gruner, Melanie A1 - Kundzer, Alena V. A1 - Volkova, Margarita V. A1 - Generalov, I. A1 - Schmidt, Carsten A1 - Schröder, Christian A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Development of a Method for Multiplex Real-Time Analysis of Enzymatic Activity on a Microbead-Chip T2 - Infection, tumors and autoimmunity, report on the 11th Dresden Symposium on Autoantibodies held in Dresden on September 1-4, 2013 Y1 - 2013 SN - 978-3-89967-881-9 SP - 275 EP - 280 PB - Pabst Science Publ. CY - Lengerich [u.a.] ER - TY - GEN A1 - Rödiger, Stefan A1 - Lehmann, Werner A1 - Frömmel, Ulrike A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Burdukiewicz, Michał A1 - Schröder, Christian A1 - Dangla, Rémi A1 - Droniou, Magali A1 - Schierack, Peter T1 - The Modi Operandi of the VideoScan Platform for the Detection and Analysis of Nucleic Acids. Life Science Day 2013 & 17. Leibniz Conference of Advanced Science, Freie Universität Berlin, 24.10.2013 Y1 - 2013 UR - https://leibniz-institut.de/Konferenzen/lsd2013/roediger_the_modi_operandi_of_the_videoscan_platform.pdf ER - TY - CHAP A1 - Rödiger, Stefan A1 - Burdukiewicz, Michał T1 - Reproducible Analysis of DNA Amplification Experiments with an Open Source Software Environment T2 - Abstractbook, 67th Annual Meeting of the German Society of Hygiene and Microbiology (DGHM), 27.-30.09.2015, Münster Y1 - 2015 UR - https://www.dghm.org/wp-content/uploads/2018/07/783_dghm2015_abstractbook_update.pdf ER - TY - GEN A1 - Kramer, Toni A1 - Schiebel, Juliane A1 - Zelck, Claudia A1 - Weinreich, Jörg A1 - Schierack, Peter A1 - Rödiger, Stefan T1 - Genotyping and gene expression analysis of putative reference genes in Enterobacteriaceae, 67th Annual Meeting of the German Society of Hygiene and Microbiology (DGHM), 27.-30.09.2015, Münster Y1 - 2015 UR - https://f1000research.com/posters/5-248 U6 - https://doi.org/10.7490/f1000research.1111354.1 ER - TY - CHAP A1 - Rödiger, Stefan A1 - Burdukiewicz, Michał A1 - Heiserich, Lisa A1 - Schierack, Peter A1 - Roggenbuck, Dirk T1 - Digital Enumeration of Double Strand Breaks via γH2AX and Aassociated biomarkers in a Computing Environment for Reproducible Research T2 - From autoantibody research to standardized diagnostic assays in the management of human diseases, report on the 12th Dresden Symposium on Autoantibodies, September 23-26, 2015 Y1 - 2015 SN - 978-3-95853-104-8 SP - S. 413 PB - Pabst Science Publishers CY - Lengerich ER - TY - GEN A1 - Rödiger, Stefan A1 - Schneider, Jens A1 - Sauer, Lysann A1 - Reddig, Annika A1 - Schierack, Peter A1 - Roggenbuck, Dirk A1 - Schröder, Christian T1 - A Multiparameter Platform for The Analysis of DNA Double-strand Breaks and Associated Biomarkers after genome Editing Austrian Biomarker Symposium (ASBD) 2016, 10.-11.03.2016, Wien, Österreich Y1 - 2016 UR - https://f1000research.com/posters/5-350 ER - TY - GEN A1 - Sauer, Lysann A1 - Rödiger, Stefan A1 - Schneider, Jens A1 - Schierack, Peter A1 - Roggenbuck, Dirk A1 - Schröder, Christian T1 - Functional Multiparameter Analy sis of Double-Strand Breaks and Associate Biomarkers during Genome Editing, Genome Editing and Gene Modulation Congress 2016, 6.–8.04.2016, Oxford, United Kingdom Y1 - 2016 UR - https://f1000research.com/posters/5-706 ER - TY - CHAP A1 - Kolenda, Rafał A1 - Burdukiewicz, Michał A1 - Szabo, M. A1 - Orlowska, Aleksandra A1 - Nitschke, Jörg A1 - Böhm, Alexander A1 - Schierack, Peter T1 - Influence of Salmonella FimH sequence variation on binding to pancreatic secretory granule membrane major glycoprotein GP2 of human and porcine origin T2 - 5th ASM Conference on Salmonella, Potsdam, 29.08-01.09.2016 Y1 - 2016 UR - https://www.asm.org/images/1903%20asm%20salmonella%20program%20web3.pdf SP - S. 125 PB - American Society for Microbiology CY - Washington, DC ER - TY - CHAP A1 - Burdukiewicz, Michał A1 - Mikolajczyk, A. A1 - Szabo, Istvan A1 - Nitschke, Jörg A1 - Böhm, Alexander A1 - Schierack, Peter A1 - Kolenda, Rafał T1 - Automated enumeration of Salmonella spp. in cell line infection assays and screening of cell line infectivity among various Salmonella isolates T2 - 5th ASM Conference on Salmonella, Potsdam, 29.08-01.09.2016 Y1 - 2016 UR - https://www.asm.org/images/1903%20asm%20salmonella%20program%20web3.pdf SP - S. 127 PB - American Society for Microbiology CY - Washington, DC ER - TY - CHAP A1 - Kolenda, Rafał A1 - Burdukiewicz, Michał A1 - Schiebel, Juliane A1 - Szabo, Istvan A1 - Orlowska, Aleksandra A1 - Nitschke, Jörg A1 - Böhm, Alexander A1 - Schierack, Peter T1 - Adhesion of Salmonella to pancreatic secretory granule membrane major glycoprotein GP2 depends on FimH sequence variation T2 - Abstractbook, Microbiology and Infection, 5th Joint Conference of the DGHM & VAAM, VAAM Annual Meeting 2017, 69th Annual Meeting of the DGHM Y1 - 2017 PB - Spektrumverlag CY - Heidelberg ER - TY - GEN A1 - Deutschmann, Claudia A1 - Dinter, Franziska A1 - Rödiger, Stefan A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Comparison of Lab and Point of Care (POC) technologies – case study for CHI3L1, Potsdam Days on Bioanalysis Y1 - 2017 UR - https://www.researchgate.net/publication/321419307 ER - TY - CHAP A1 - Rödiger, Stefan A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Stavitskaya, Luba A1 - Gruner, Melanie A1 - Kundzer, Alena V. A1 - Volkova, Margarita V. A1 - Generalov, I. A1 - Schmidt, Carsten A1 - Schröder, Christian A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Development of a Method for Multiplex Real-Time Analysis of Enzymati c Activity on a Microbead-Chip. T2 - Infections, Tumors and Autoimmunity, Report on the 11th Dresden Symposium on Autoantibodies held in Dresden on September 01. - 04., 2013 Y1 - 2013 SN - 978-3-89967-881-9 SP - 280 PB - Pabst Science Publ. CY - Lengerich ER - TY - CHAP A1 - Rödiger, Stefan A1 - Hanschmann, Henning A1 - Steidle, Michael A1 - Schröder, Christian A1 - Schierack, Peter A1 - Lehmann, Werner T1 - Detection of Borrelia, Bordetella and HPV with the LoopTag Real-Time PCR Probe System T2 - International journal of medical microbiology Y1 - 2013 SN - 1618-0607 SN - 1438-4221 N1 - 65. Jahrestagung der Deutschen Gesellschaft für Hygiene und Mikrobiologie e.V. und der Jahrestagung der Deutschen Gesellschaft für Infektiologie (DGI) e.V., Universität Rostock, 22.09. – 25.09.20 13, Rostock VL - 303 IS - Supplement 1 SP - 12 EP - 13 ER - TY - CHAP A1 - Rödiger, Stefan T1 - Applications of a Multipurpose Platf orm for the Detection and Analysis of Biomolecules T2 - BioSensor, 9. Deutsches BioSensor Symposium, 11.-13. März 2015 Y1 - 2015 PB - München ER - TY - GEN A1 - Spiess, Andrej-Nikolai A1 - Rödiger, Stefan A1 - Burdukiewicz, Michał A1 - Volksdorf, Thomas A1 - Tellinghuisen, Joel T1 - System-specific periodicity in quantitative real-time polymerase chain reaction data questions threshold-based quantitation T2 - Scientific Reports Y1 - 2016 U6 - https://doi.org/10.1038/srep38951 SN - 2045-2322 VL - 6 ER - TY - GEN A1 - Burdukiewicz, Michał A1 - Sobczyk, Piotr A1 - Rödiger, Stefan A1 - Duda-Madej, Anna A1 - Mackiewicz, Pawel A1 - Kotulska, Malgorzata T1 - Amyloidogenic motifs revealed by n-gram analysis T2 - Scientific Reports Y1 - 2017 U6 - https://doi.org/10.1038/s41598-017-13210-9 SN - 2045-2322 VL - 7 ER - TY - GEN A1 - Rödiger, Stefan A1 - Liefold, Marius A1 - Ruhe, Madeleine A1 - Reinwald, Mark A1 - Beck, Eberhard A1 - Deckert, P. Markus T1 - Quantification of DNA double-strand breaks in peripheral blood mononuclear cells from healthy donors exposed to bendamustine by an automated H2AX assay- an exploratory study T2 - Journal of Laboratory and Precision Medicine Y1 - 2018 U6 - https://doi.org/10.21037/jlpm.2018.04.10 SN - 2519-9005 VL - 3 IS - 47 ER - TY - GEN A1 - Dinter, Franziska A1 - Deutschmann, Claudia A1 - Schierack, Peter A1 - Dame, Gregory A1 - Rödiger, Stefan T1 - Integration of Cardiovascular Disease Biomarkers in a Microfluidic Microbead Chip Y1 - 2017 UR - https://f1000research.com/posters/6-2136 N1 - Potsdam Days on Bioanalysis 2017 ER - TY - GEN A1 - Schiebel, Juliane A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Burdukiewicz, Michał A1 - Weinreich, Jörg A1 - Ali, Aamir A1 - Roggenbuck, Dirk A1 - Rödiger, Stefan A1 - Schierack, Peter T1 - Genotypic and phenotypic characteristics in association with biofilm formation in different pathotypes of human clinical Escherichia coli isolates T2 - Applied and Environmental Microbiology Y1 - 2017 U6 - https://doi.org/10.1128/AEM.01660-17 SN - 1098-5336 SN - 0099-2240 VL - 83 IS - 24 ER - TY - GEN A1 - Liebsch, Claudia A1 - Rödiger, Stefan A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Weinreich, Jörg A1 - Fruth, Angelika A1 - Roggenbuck, Dirk A1 - Lehmann, Werner A1 - Schedler, Uwe A1 - Juretzek, Thomas A1 - Schierack, Peter T1 - Solid-phase microbead array for multiplex O-serotyping of Escherichia coli T2 - Microchimica Acta Y1 - 2017 U6 - https://doi.org/10.1007/s00604-017-2088-4 SN - 0026-3672 SN - 1436-5073 VL - 184 IS - 5 SP - 1405 EP - 1415 ER - TY - GEN A1 - Burdukiewicz, Michał A1 - Spiess, Andrej-Nikolai A1 - Blagodatskikh, Konstantin A. A1 - Lehmann, Werner A1 - Schierack, Peter A1 - Rödiger, Stefan T1 - Algorithms for automated detection of hook effect-bearing amplification curves T2 - Biomolecular Detection and Quantification Y1 - 2018 U6 - https://doi.org/10.1016/j.bdq.2018.08.001 SN - 2214-7535 VL - 16 SP - 1 EP - 4 ER - TY - GEN A1 - Deutschmann, Claudia A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - The loss of tolerance to CHI3L1-A putative role in inflammatory bowel disease? T2 - Clinical Immunology Y1 - 2018 U6 - https://doi.org/10/gftf2k SN - 1521-6616 VL - 199 SP - 12 EP - 17 ER - TY - GEN A1 - Reddig, Annika A1 - Rübe, Claudia E. A1 - Rödiger, Stefan A1 - Schierack, Peter A1 - Reinhold, Dirk A1 - Roggenbuck, Dirk T1 - DNA damage assessment and potential applications in laboratory diagnostics and precision medicine T2 - Journal of Laboratory and Precision Medicine Y1 - 2018 U6 - https://doi.org/10.21037/jlpm.2018.03.06 SN - 2519-9005 VL - 3 ER - TY - GEN A1 - Sowa, Mandy A1 - Reddig, Annika A1 - Schierack, Peter A1 - Reinhold, Dirk A1 - Roggenbuck, Dirk T1 - Phosphorylated histone 2AX foci determination in capillary blood mononuclear cells T2 - Journal of Laboratory and Precision Medicine Y1 - 2018 U6 - https://doi.org/10.21037/jlpm.2018.04.02 SN - 2519-9005 VL - 3 ER - TY - GEN A1 - Schneider, Jens A1 - Weiss, Romano A1 - Ruhe, Madeleine A1 - Jung, Tobias A1 - Roggenbuck, Dirk A1 - Stohwasser, Ralf A1 - Schierack, Peter A1 - Rödiger, Stefan T1 - Open source bioimage informatic tools for the analysis of DNA damage and associated biomarkers T2 - Journal of Laboratory and Precision Medicine KW - bioimage informatics KW - DNA damage response (DDR) KW - double-strand break (DSB) KW - immunofluorescence microscopy KW - open source software KW - proteasome activator PA28gamma Y1 - 2019 U6 - https://doi.org/10.21037/jlpm.2019.04.05 SN - 2519-9005 VL - 4 ER - TY - GEN A1 - Ruhe, Madeleine A1 - Rabe, Dominik A1 - Jurischka, Christoph A1 - Schröder, Julia A1 - Schierack, Peter A1 - Deckert, P. Markus A1 - Rödiger, Stefan T1 - Molecular biomarkers of DNA damage in diffuse large-cell lymphoma-a review T2 - Journal of Laboratory and Precision Medicine Y1 - 2019 U6 - https://doi.org/10.21037/jlpm.2019.01.01 SN - 2519-9005 VL - 4 ER - TY - GEN A1 - Umair, Muhammad A1 - Mohsin, Mashkoor A1 - Ali, Qasim A1 - Qamar, Muhammad U. A1 - Raza, Shahbaz A1 - Ali, Aamir A1 - Guenther, Sebastian A1 - Schierack, Peter T1 - Prevalence and Genetic Relatedness of Extended Spectrum-ß-Lactamase-Producing Escherichia coli Among Humans, Cattle, and Poultry in Pakistan T2 - Microbial drug resistance Y1 - 2019 U6 - https://doi.org/10.1089/mdr.2018.0450 SN - 1931-8448 SN - 1076-6294 VL - 25 IS - 9 SP - 1374 EP - 1381 ER - TY - GEN A1 - Wajid, Muhammad A1 - Awan, Asad Bashir A1 - Saleemi, Muhammad Kashif A1 - Weinreich, Jörg A1 - Schierack, Peter A1 - Sarwar, Yasra A1 - Ali, Aamir T1 - Multiple Drug Resistance and Virulence Profiling of Salmonella enterica Serovars Typhimurium and Enteritidis from Poultry Farms of Faisalabad, Pakistan T2 - Microbial Drug Resistance Y1 - 2019 U6 - https://doi.org/10.1089/mdr.2018.0121 SN - 1931-8448 SN - 1076-6294 VL - 25 IS - 1 SP - 133 EP - 142 ER - TY - GEN A1 - Thaler, Markus A. A1 - Bietenbeck, Andreas A1 - Steigerwald, Udo A1 - Büttner, Thomas A1 - Schierack, Peter A1 - Lindhoff-Last, Edelgard A1 - Roggenbuck, Dirk A1 - Luppa, Peter B. T1 - Evaluation of the sensitivityficity of a novel line immunoassay for the detection of criteria and non-criteria antiphospholipid antibodies in comparison to establishedELISAs T2 - PLoS One Y1 - 2019 U6 - https://doi.org/10.1371/journal.pone.0220033 SN - 1932-6203 VL - 14 IS - 7 ER - TY - GEN A1 - Akhtar, Nasrin A1 - Ghauri, M. A. A1 - Parveen, Sana A1 - Farooq, M. A1 - Ali, Aamir A1 - Schierack, Peter T1 - Comparative Analysis of Draft Genome Sequence of Rhodococcus sp. Eu-32 with Other Rhodococcus Species for Its Taxonomie Status and Sulfur Metabolism Potential T2 - Current microbiology Y1 - 2019 U6 - https://doi.org/10.1007/s00284-019-01737-1 SN - 1432-0991 SN - 0343-8651 VL - 76 IS - 10 SP - 1207 EP - 1214 ER - TY - GEN A1 - Olowe, Olugbenga Adekunle A1 - Adefioye, Olusolabomi J. A1 - Ajayeoba, T. A. A1 - Schiebel, Juliane A1 - Weinreich, Jörg A1 - Ali, Aamir A1 - Burdukiewicz, Michał A1 - Rödiger, Stefan A1 - Schierack, Peter T1 - Phylogenetic grouping and biofilm formation of Multidrug Resistant Escherichia coli Isolates from Humans, Animals and Food products in South-west Nigeria T2 - Scientific African Y1 - 2019 U6 - https://doi.org/10.1016/j.sciaf.2019.e00158 SN - 2468-2276 VL - 6 ER - TY - GEN A1 - Choi, Youngeun A1 - Schmidt, Carsten A1 - Tinnefeld, Philip A1 - Bald, Ilko A1 - Rödiger, Stefan T1 - A new reporter design based on DNA origami nanostructures for quantification of short oligonucleotides using microbeads T2 - Scientific Reports Y1 - 2019 U6 - https://doi.org/10.1038/s41598-019-41136-x. SN - 2045-2322 IS - 9 ER - TY - GEN A1 - Herrmann, Anna A1 - Rödiger, Stefan A1 - Schmidt, Carsten A1 - Schierack, Peter A1 - Schedler, Uwe T1 - Spatial Separation of Microbeads into Detection Levels by a Bioorthogonal Porous Hydrogel for Size-Selective Analysis und Increased Multiplexicity T2 - Analytical chemistry Y1 - 2019 U6 - https://doi.org/10.1021/acs.analchem.9b01586. SN - 1520-6882 SN - 0003-2700 VL - 91 IS - 13 SP - 8484 EP - 8491 ER - TY - GEN A1 - Awan, Asad Bashir A1 - Schiebel, Juliane A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Sarwar, Yasra A1 - Schierack, Peter A1 - Ali, Aamir T1 - Association of biofilm formation and cytotoxic potential with multidrug resistance in clinical isolates of Pseudomonas aeruginosa T2 - EXCLI journal Y1 - 2019 SN - 1611-2156 VL - 18 SP - 79 EP - 90 ER - TY - GEN A1 - Deutschmann, Claudia A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - The loss of tolerance to CHI3L1-A putative role in inflammatory bowel disease? T2 - Clinical Immunology Y1 - 2019 U6 - https://doi.org/10.1016/j.clim.2018.12.005 SN - 1521-6616 SN - 1521-7035 VL - 199 SP - 12 EP - 17 ER - TY - GEN A1 - Dinter, Franziska A1 - Burdukiewicz, Michał A1 - Schierack, Peter A1 - Lehmann, Werner A1 - Nestler, Jörg A1 - Dame, Gregory A1 - Rödiger, Stefan T1 - Simultaneous detection and quantification of DNA and protein biomarkers in spectrum of cardiovascular diseases in a microfluidic microbead chip T2 - Analytical and Bioanalytical Chemistry Y1 - 2019 U6 - https://doi.org/10.1007/s00216-019-02199-x SN - 1618-2650 SN - 1618-2642 VL - 411 IS - 29 SP - 7725 EP - 7735 ER - TY - GEN A1 - Deutschmann, Claudia A1 - Sowa, Mandy A1 - Murugaiyan, Jayaseelan A1 - Roessler, Uwe A1 - Röber, Nadja A1 - Conrad, Karsten A1 - Laass, Martin W. A1 - Bogdanos, Dimitrios Petrou A1 - Sipeki, Nora A1 - Papp, Maria A1 - Rödiger, Stefan A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Identification of Chitinase-3-Like Protein 1 as a Novel Neutrophil Antigenic Target in Crohn’s Disease T2 - Journal of Crohn's and Colitis N2 - Background and Aims There is an increasing incidence of inflammatory bowel disease [IBD]. Autoimmune responses are involved in the pathophysiology of IBD, but their underlying pathways and target antigens have not yet been fully elucidated. Methods Autoantigenic targets in IBD were identified after separation of whole cell proteins isolated from neutrophils using two-dimensional electrophoresis and matrix assisted laser desorption ionization – time of flight mass spectrometry-based protein identification of the spots that displayed Western blotting signals with anti-neutrophil cytoplasmic antibody-positive sera. The prevalence of IgG, IgA and secretory IgA [sIgA] to chitinase 3-like protein 1 [CHI3L1] was analysed by enzyme-linked immunosorbent assays using recombinant CHI3L1 in 110 patients with Crohn’s disease [CD], 95 with ulcerative colitis [UC], 126 with coeliac disease [CeD] and 86 healthy controls [HCs]. Results The 18-glycosylhydrolase family member CHI3L1 was identified as a neutrophil autoantigenic target. CD patients displayed significantly higher levels of IgG to CHI3L1 than patients with UC and CeD (p < 0.0001, respectively). IgA and sIgA to CHI3L1 was significantly higher in CD than in UC, CeD and HCs [p < 0.0001, respectively]. IgA and sIgA to CHI3L1 demonstrated the highest prevalence in CD [25.5%, 28/110; and 41.8%%, 46/110] compared to HCs [2.3%, 2/86; and 4.7%%, 4/86; p = 0.0015 and p < 0.0001] and are associated with a more complicated progression of CD. Conclusion CHI3L1 is a novel neutrophil autoantigenic target in CD. IgA and sIgA to CHI3L1 may serve as novel markers for CD and may facilitate the serological diagnosis of IBD. Y1 - 2019 U6 - https://doi.org/10.1093/ecco-jcc/jjz012 SN - 1876-4479 SN - 1873-9946 VL - 13 IS - 7 SP - 894 EP - 904 ER - TY - GEN A1 - Sowa, Mandy A1 - Murugaiyan, Jayaseelan A1 - Conrad, Karsten A1 - Laass, Martin W. A1 - Bogdanos, Dimitrios Petrou A1 - Papp, Maria A1 - Rödiger, Stefan A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - A novel neutrophil autoantigenic target in inflammatory bowel disease Y1 - 2018 UR - https://www.researchgate.net/publication/335889899_A_novel_neutrophil_autoantigenic_target_in_inflammatory_bowel_disease ER - TY - GEN A1 - Jurischka, Christoph A1 - Dinter, Franziska A1 - Efimova, Anastasia A1 - Weiss, Romano A1 - Schiebel, Juliane A1 - Schulz, Christian A1 - Fayziev, Bekzodjon A1 - Schierack, Peter A1 - Fischer, Thomas A1 - Rödiger, Stefan T1 - An explorative study of polymers for 3D printing of bioanalytical test systems T2 - Clinical Hemorheology and Microcirculation N2 - Background: The 3D printing is relevant as a manufacturing technology of functional models for forensic, pharmaceutical and bioanalytical applications such as drug delivery systems, sample preparation and point-of-care tests. Objective: Melting behavior and autofluorescence of materials are decisive for optimal printing and applicability of the product which are influenced by varying unknown additives. Methods: We have produced devices for bioanalytical applications from commercially available thermoplastic polymers using a melt-layer process. We characterized them by differential scanning calorimetry, fluorescence spectroscopy and functional assays (DNA capture assay, model for cell adhesion, bacterial adhesion and biofilm formation test). Results: From 14 tested colored, transparent and black materials we found only deep black acrylonitrile-butadiene-styrene (ABS) and some black polylactic acid (PLA) useable for fluorescence-based assays, with low autofluorescence only in the short-wave range of 300-400 nm. PLA was suitable for standard bioanalytical purposes due to a glass transition temperature of approximately 60°C, resistance to common laboratory chemicals and easy print processing. For temperature-critical methods, such as hybridization reactions up to 90°C, ABS was better suited. Conclusions: Autofluorescence was not a disadvantage per se but can also be used as a reference signal in assays. The rapid development of individual protocols for sample processing and analysis required the availability of a material with consistent quality over time. For fluorescence-based assays, the use of commercial standard materials did not seem to meet this requirement. KW - Thermal analysis KW - 3D Printing Y1 - 2020 UR - https://pubmed.ncbi.nlm.nih.gov/31929149/ U6 - https://doi.org/10.3233/CH-190713 SN - 1875-8622 VL - 75 IS - 1 SP - 57 EP - 84 ER - TY - GEN A1 - Lopens, Steffi A1 - Krawczyk, Marcin A1 - Papp, Maria A1 - Milkiewicz, Piotr A1 - Schierack, Peter A1 - Liu, Yudong A1 - Wunsch, Ewa A1 - Conrad, Karsten A1 - Roggenbuck, Dirk T1 - The search for the Holy Grail: autoantigenic targets in primary sclerosing cholangitis asscociated with diesease phenotype an neoplasia T2 - Autoimmunity Highlights Y1 - 2020 U6 - https://doi.org/10.1186/s13317-020-00129-x SN - 2038-3274 SN - 2038-0305 VL - 11 ER - TY - GEN A1 - Whale, Alexandra S. A1 - Spiegelaere, Ward De A1 - Trypsteen, W. A1 - Nour, Afif Abdel A1 - Bae, Young-Kyung A1 - Benes, Vladimir A1 - Cleveland, Megan A1 - Devonshire, Alison S. A1 - Dong, Lianhua A1 - Drandi, Daniela A1 - Foy, Carole A. A1 - Garson, Jeremy A. A1 - He, Hua-Jun A1 - Hellemans, Jan A1 - Kubista, Mikael A1 - Lievens, Antoon A1 - Makrigiogos, Mike G. A1 - Müller, Reinhold D. A1 - Nolan, Tania A1 - O'Sullivan, Denise M. A1 - Pfaffl, Michael W. A1 - Rödiger, Stefan A1 - Romsos, Erica L. A1 - Shipley, Gregory L. A1 - Taly, Valerie A1 - Untergasser, Andreas A1 - Wittwer, Carl T. A1 - Bustin, Stephen A. A1 - Vandesompele, Jo A1 - Huggett, Jim F. T1 - The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020 T2 - Clinical Chemistry Y1 - 2020 U6 - https://doi.org/10.1093/clinchem/hvaa125 SN - 0009-9147 SN - 1530-8561 VL - 66 IS - 8 SP - 1012 EP - 1029 ER - TY - GEN A1 - Schmidt, Carsten A1 - Schierack, Peter A1 - Gerber, Ulrike A1 - Schröder, Christian A1 - Choi, Youngeun A1 - Bald, Ilko A1 - Lehmann, Werner A1 - Rödiger, Stefan T1 - Streptavidin Homologues for Applications on Solid Surfaces at High Temperatures T2 - Langmuir Y1 - 2020 U6 - https://doi.org/10.1021/acs.langmuir.9b02339 SN - 1520-5827 SN - 0743-7463 VL - 36 IS - 2 SP - 628 EP - 636 ER - TY - GEN A1 - Burdukiewicz, Michał A1 - Sidorczuk, Katarzyna A1 - Rafacz, Dominik A1 - Pietluch, Filip A1 - Chilimoniuk, Jarosław A1 - Rödiger, Stefan A1 - Przemysław, Gagat T1 - Proteomic Screening for Prediction and Design of Antimicrobial Peptides with AmpGram T2 - International Journal of Molecular Sciences Y1 - 2020 U6 - https://doi.org/10.3390/ijms21124310 SN - 1422-0067 SN - 1661-6596 VL - 21 IS - 12 ER - TY - GEN A1 - Volkov, Ilan A1 - Seguro, Luciana A1 - Leion, Elaine P. A1 - Kovács, László A1 - Roggenbuck, Dirk A1 - Schierack, Peter A1 - Gilburd, Boris A1 - Doria, Andrea A1 - Tektonidou, Maria G. A1 - Agmon-Levin, Nancy T1 - Profiles of criteria and non-criteria anti-phospholipid autoantibodies are associated with clinical phenotypes of the antiphospholipid syndrome T2 - Autoimmunity Highlights Y1 - 2020 U6 - https://doi.org/10.1186/s13317-020-00131-3 SN - 2038-3274 SN - 2038-0305 VL - 11 SP - 1 EP - 8 ER - TY - GEN A1 - Ali, Aamir A1 - Kolenda, Rafał A1 - Khan, Muhammad Moman A1 - Weinreich, Jörg A1 - Li, Ganwu A1 - Wieler, Lothar H. A1 - Tedin, Karsten A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Novel Avian Pathogenic Escherichia coli Genes Responsible for Adhesion to Chicken and Human Cell Lines T2 - Applied and Environmental Microbiology Y1 - 2020 U6 - https://doi.org/10.1128/AEM.01068-20 SN - 1098-5336 VL - 86 IS - 20 ER - TY - GEN A1 - Schierack, Peter A1 - Heiden, Stefan E. A1 - Khan, Muhammad Moman A1 - Nikolaus, Lena A1 - Kolenda, Rafał A1 - Stubbe, Michael A1 - Lkhagvasuren, Davaa A1 - Rödiger, Stefan A1 - Guenther, Sebastian A1 - Schaufler, Katharina T1 - Genomic and Phenotypic Analysis of an ESBL-Producing E. coli ST1159 Clonal Lineage From Wild Birds in Mongolia T2 - Frontiers in Microbiology Y1 - 2020 U6 - https://doi.org/10.3389/fmicb.2020.01699 SN - 1664-302X VL - 11 ER - TY - GEN A1 - Reimann, Ronny A1 - Zeng, Bo A1 - Jakopec, Martin A1 - Burdukiewicz, Michał A1 - Petrick, Ingolf A1 - Schierack, Peter A1 - Rödiger, Stefan T1 - Classification of dead and living microalgae Chlorella vulgaris by bioimage informatics an machine learning T2 - Algal Research Y1 - 2020 U6 - https://doi.org/10.1016/j.algal.2020.101908 SN - 2211-9264 VL - 48 ER - TY - GEN A1 - Burdukiewicz, Michał A1 - Sidorczuk, Katarzyna A1 - Rafacz, Dominik A1 - Pietluch, Filip A1 - Bakała, Mateusz A1 - Słowik, Jadwiga A1 - Gagat, Przemysław T1 - CancerGram: An Effective Classifier for Differentiating Anticancer from Antimicrobial Peptides T2 - Pharmaceutics Y1 - 2020 U6 - https://doi.org/10.3390/pharmaceutics1211145 SN - 1999-4923 VL - 12 IS - 11 ER - TY - GEN A1 - Roggenbuck, Dirk A1 - Elmont, Emilien A1 - Reinhold, Dirk A1 - Schierack, Peter A1 - Conrad, Karsten A1 - Boucraut, Joseph T1 - Autoimmune Perpheral Neuropathies and Contribution of Antiganglioside/Sulphatide Autoantibody Testing T2 - Mediterranean Jounal of Rheumatology Y1 - 2020 U6 - https://doi.org/10.31138/mjr.31.1.10 SN - 2529-198X VL - 31 IS - 1 SP - 10 EP - 18 ER - TY - GEN A1 - Deutschmann, Claudia A1 - Roggenbuck, Dirk A1 - Schierack, Peter A1 - Rödiger, Stefan T1 - Autoantibody testing by enzyme-linked immunosorbent assay-a case in which the solid phase decides on success and failure T2 - Heliyon Y1 - 2020 U6 - https://doi.org/10.1016/j.heliyon.2020.e03270 SN - 2405-8440 VL - 6 IS - 1 ER - TY - GEN A1 - Schiebel, Juliane A1 - Noack, Jonas A1 - Rödiger, Stefan A1 - Kammel, Anne A1 - Menzel, Friederike A1 - Schwibbert, Karin A1 - Weise, Matthias A1 - Weiss, Romano A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Elimport, Alexey A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Analysis of three-dimensional biofilms on different material surfaces T2 - Biomaterials Science Y1 - 2020 U6 - https://doi.org/10.1039/D0BM00455C SN - 2047-4849 VL - 8 IS - 12 SP - 3500 EP - 3510 ER - TY - GEN A1 - Dinter, Franziska A1 - Jurischka, Christoph A1 - Weiss, Romano A1 - Rödiger, Stefan T1 - Multiparametrische Antikörperdetektion für die Diagnostik T2 - BIOspektrum Y1 - 2020 U6 - https://doi.org/10.1077/s12268-020-1375-5 SN - 1868-6249 SN - 0947-0867 VL - 26 IS - 3 SP - 265 EP - 267 ER - TY - GEN A1 - Liedtke, Victoria A1 - Schröder, Christian A1 - Roggenbuck, Dirk A1 - Weiss, Romano A1 - Stohwasser, Ralf A1 - Schierack, Peter A1 - Rödiger, Stefan A1 - Schenk, Lysann T1 - LEDGF/p75 is required for an efficient DNA damage response T2 - International Journal of Molecular Sciences KW - LEDGF KW - CRISPR/Cas9 KW - DNA damage signaling KW - gH2AX KW - ubiquitination Y1 - 2021 U6 - https://doi.org/10.3390/ijms22115866 VL - 22 IS - 11 SP - 1 EP - 16 ER - TY - GEN A1 - Kolenda, Rafał A1 - Burdukiewicz, Michał A1 - Wimonć, Marcjanna A1 - Aleksandrowicz, Adrianna A1 - Ali, Aamir A1 - Szabo, Istvan A1 - Tedin, Karsten A1 - Bartholdson, Scott J. A1 - Pickhard, Derek A1 - Schierack, Peter T1 - Identification of Natural Mutations Responsible for Altered Infection Phenotypes of Salmonella enterica Clinical Isolates by Using Cell Line Infection Screens T2 - Applied and Environmental Microbiology Y1 - 2021 U6 - https://doi.org/10.1128/AEM.02177-20 SN - 1098-5336 VL - 87 IS - 2 ER - TY - GEN A1 - Comabella, Manual A1 - Deutschmann, Claudia A1 - Midaglia, Luciana A1 - Schierack, Peter A1 - Martínez, Júlia A1 - Roggenbuck, Dirk A1 - Montalban, Xavier T1 - Chitinase 3-like 1 is not a target antigen in patients with multiple sclerosis T2 - Multiple Sclerosis Journal Y1 - 2021 U6 - https://doi.org/10.1177/1352458520980141 SN - 1477-0970 SN - 1352-4585 VL - 27 IS - 9 SP - 1455 EP - 1457 ER - TY - GEN A1 - Schmidt, Jonas A1 - Berghaus, Sandro A1 - Blessing, Frithjof A1 - Wenzel, Folker A1 - Herbeck, Holger A1 - Blessing, Josef A1 - Schierack, Peter A1 - Rödiger, Stefan A1 - Roggenbuck, Dirk T1 - Serological and viral genetic features of patients with COVID-19 in a selected German patient cohort-correlation with disease characteristics T2 - GeroScience Y1 - 2021 U6 - https://doi.org/10.1007/s11357-021-00443-w SN - 2509-2723 SN - 2509-2715 VL - 43 IS - 5 SP - 2249 EP - 2264 ER - TY - GEN A1 - Adefioye, Olusolabomi J. A1 - Weinreich, Jörg A1 - Rödiger, Stefan A1 - Schierack, Peter A1 - Olowe, Olugbenga Adekunle T1 - Phylogenetic Characterization and Multilocus Sequence Typing of Extended-Spectrum Beta Lactamase-Producing Escherichia coli from Food-Producing Animals, Beef, and Humans in Southwest Nigeria T2 - Microbial Drug Resistance Y1 - 2021 U6 - https://doi.org/10.1089/mdr.2019.0397 SN - 1931-8448 SN - 1076-6294 VL - 27 IS - 1 SP - 111 EP - 120 ER - TY - GEN A1 - Chilimoniuk, Jarosław A1 - Gosiewska, Alicja A1 - Słowik, Jadwiga A1 - Weiss, Romano A1 - Deckert, P. Markus A1 - Rödiger, Stefan A1 - Burdukiewicz, Michał T1 - coutfitteR: efficient selection of count distributions to assess DNA damage T2 - Annals of Translational Medicine Y1 - 2021 U6 - https://doi.org/10.21037/atm-20-6363 SN - 2305-5847 VL - 9 IS - 7 ER - TY - GEN A1 - Hanschmann, Henning A1 - Rödiger, Stefan A1 - Kramer, Toni A1 - Hanschmann, Katrin A1 - Steidle, Michael A1 - Fingerle, Volker A1 - Schmidt, Carsten A1 - Lehmann, Werner A1 - Schierack, Peter T1 - LoopTag FRET Probe System for Multiplex qPCR Detection of Borrelia Species T2 - Life Y1 - 2021 U6 - https://doi.org/10.3390/life11111163 SN - 2075-1729 VL - 11 IS - 11 ER - TY - GEN A1 - Schmidt, Carsten A1 - Borcherding, Heike A1 - Thiele, Thomas A1 - Schedler, Uwe A1 - Werner, Franziska A1 - Rödiger, Stefan A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Fluorescence-encoded poly (methyl metharcylate) nanoparticles for a lateral flow assay detecting IgM autoantibodies in rheumatoid arthritis T2 - Analytical biochemistry Y1 - 2021 U6 - https://doi.org/10.1016/journal.ppat.1010118 SN - 1096-0309 VL - Vol. 633 ER - TY - GEN A1 - Emmenegger, Marc A1 - Kumar, Sreedhar Saseendran A1 - Emmenegger, Vishalini A1 - Malinauskas, Tomas A1 - Büttner, Thomas A1 - Rose, Laura A1 - Schierack, Peter A1 - Sprinzl, Martin F. A1 - Sommer, Clemens J. A1 - Lackner, Karl J. A1 - Aguzzi, Adriano A1 - Roggenbuck, Dirk A1 - Frauenknecht, Katrin B. M. T1 - Anti-prothrombin autoantibodies enriched after infection with SARS-CoV-2 and influenced by strength of antibody response against SARS-CoV-2 proteins T2 - PLoS pathogens Y1 - 2021 U6 - https://doi.org/10.1371/journal.ppat.1010118 SN - 1553-7374 VL - 17 IS - 12 ER - TY - GEN A1 - Khan, Muhammad Moman A1 - Kolenda, Rafał A1 - Schierack, Peter A1 - Weinreich, Jörg A1 - Rödiger, Stefan A1 - Schierack, Jakob A1 - Stubbe, Michael A1 - Lkhagvasuren, Davaa A1 - Guenther, Sebastian A1 - Schaufler, Katharina T1 - Investigation of Commensal Escherichia coli Populations of Cormorant Hatchlings in the Absence of Anthropogenic Impacts in Remote Areas of West Mongolia T2 - Microorganisms Y1 - 2021 U6 - https://doi.org/10.3390/microorganisms9020372 SN - 2076-2607 VL - 9 IS - 2 ER - TY - GEN A1 - Awan, Asad Bashir A1 - Yan, Aixin A1 - Sarwar, Yasra A1 - Schierack, Peter A1 - Ali, Aamir T1 - Detection of synergistic antimicrobial resistance mechanisms in clinical isolates of Pseudomonas aeruginosa from post-operative wound infections T2 - Applied Microbiology and Biotechnology Y1 - 2021 U6 - https://doi.org/10.1007/s00253-021-11680-6 SN - 1432-0614 VL - 105 IS - 2 SP - 9321 EP - 9332 ER - TY - GEN A1 - Roggenbuck, Johannes J. A1 - Zarske, Grit A1 - Schierack, Peter A1 - Wunderlich, Gerd A1 - Conrad, Karsten A1 - Kotzerke, Joerg A1 - Roggenbuck, Dirk A1 - Zöphel, Klaus T1 - Third generation radioimmunoassay (RIA) for TSH receptor autoantibodies (TRAb) - one step less, similar results? T2 - Nuklearmedizin Y1 - 2021 U6 - https://doi.org/10.1055/a-1277-5972 SN - 0029-5566 VL - 60 IS - 1 SP - 38 EP - 46 ER - TY - GEN A1 - Schmidt, Jonas A1 - Berghaus, Sandro A1 - Blessing, Frithjof A1 - Wenzel, Folker A1 - Herbeck, Holger A1 - Blessing, Josef A1 - Schierack, Peter A1 - Rödiger, Stefan A1 - Roggenbuck, Dirk T1 - A semi-automated, isolation-free, high-throughput SARS-CoV-2 reverse transcriptase (RT) loop-mediated isothermal amplification (LAMP) test T2 - Scientific reports Y1 - 2021 U6 - https://doi.org/10.1038/s41598-021-00827-0 SN - 2045-2322 VL - 11 IS - 1 ER - TY - GEN A1 - Bartlitz, Christin A1 - Kolenda, Rafał A1 - Chilimoniuk, Jarosław A1 - Grzymajlo, Krzysztof A1 - Rödiger, Stefan A1 - Bauerfeind, Rolf A1 - Ali, Aamir A1 - Tchesnokovag, Veronika A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Adhesion of Enteropathogenic, Enterotoxigenic, and Commensal Escherichia coli to the Major Zymogen Granule Membrane Glyoprotein 2 T2 - Applied abd Environmental Microbiology Y1 - 2022 U6 - https://doi.org/10.1128/aem.02279-21 SN - 1098-5536 VL - 88 IS - 5 ER - TY - GEN A1 - Schmidt, Carsten A1 - Kammel, Anne A1 - Tanner, Julian A. A1 - Kinghorn, Andrew B. A1 - Khan, Muhammad Moman A1 - Lehmann, Werner A1 - Menger, Marcus A1 - Schedler, Uwe A1 - Schierack, Peter A1 - Rödiger, Stefan T1 - A Multiparametic Fluorescence Assay for Screening Aptamer-Protein Interactions Based on Microbeads T2 - Scientific Reports Y1 - 2022 U6 - https://doi.org/10.1038/s41598-022-06817-0 SN - 2045-2322 VL - 12 ER - TY - GEN A1 - Sydow, Katharina A1 - Eger, Elias A1 - Schwabe, Michael A1 - Heiden, Stefan E. A1 - Bohnert, Jürgen A. A1 - Franzenburg, Sören A1 - Jurischka, Christoph A1 - Schierack, Peter A1 - Schaufler, Katharina T1 - Geno- and Phenotypic Characteristics of a Klebsiella pneumoniae ST20 Isolate with Unusual Colony Morphology T2 - Microorganisms N2 - Klebsiella pneumoniae is a common member of the intestinal flora of vertebrates. In addition to opportunistic representatives, hypervirulent (hvKp) and antibiotic-resistant K. pneumoniae (ABR-Kp) occur. While ABR-Kp isolates often cause difficult-to-treat diseases due to limited therapeutic options, hvKp is a pathotype that can infect healthy individuals often leading to recurrent infection. Here, we investigated the clinical K. pneumoniae isolate PBIO3459 obtained from a blood sample, which showed an unusual colony morphology. By combining whole-genome and RNA sequencing with multiple in vitro and in vivo virulence-associated assays, we aimed to define the respective Klebsiella subtype and explore the unusual phenotypic appearance. We demonstrate that PBIO3459 belongs to sequence type (ST)20 and carries no acquired resistance genes, consistent with phenotypic susceptibility tests. In addition, the isolate showed low-level virulence, both at genetic and phenotypic levels. We thus suggest that PBIO3459 is an opportunistic (commensal) K. pneumoniae isolate. Genomic comparison of PBIO3459 with closely related ABR-Kp ST20 isolates revealed that they differed only in resistance genes. Finally, the unusual colony morphology was mainly associated with carbohydrate and amino acid transport and metabolism. In conclusion, our study reveals the characteristics of a Klebsiella sepsis isolate and suggests that opportunistic representatives likely acquire and accumulate antibiotic resistances that subsequently enable their emergence as ABR-Kp pathogens. KW - virulence KW - next-generation sequencing KW - biofilm KW - exopolysaccharides Y1 - 2022 U6 - https://doi.org/10.3390/microorganisms10102063 SN - 2076-2607 VL - 10 IS - 10 ER - TY - GEN A1 - Lopens, Steffi A1 - Wunsch, Ewa A1 - Milkiewicz, Malgorzata A1 - Röber, Nadja A1 - Zarske, Grit A1 - Nasser, Abdullah A1 - Conrad, Karsten A1 - Laass, Martin W. A1 - Rödiger, Stefan A1 - Krawczyk, Marcin A1 - Roggenbuck, Dirk A1 - Milkiewicz, Piotr T1 - PR3-ANCAs Detected by Third-Generation ELISA Predicts Severe Disease and Poor Survival in Primary Sclerosing Cholangitis T2 - Diagnostics N2 - A highly sensitive detection of anti-neutrophil cytoplasmic antibodies to serine proteinase-3 (PR3-ANCAs) aids in the serological diagnosis of autoimmune liver disorders and the prediction of severity in primary sclerosing cholangitis (PSC). Here, we evaluate a novel third-generation ELISA for the detection of PR3-ANCAs. In total, 309 patients with PSC, 51 with primary biliary cholangitis (PBC), and 120 healthy blood donors (BD) were analyzed. For the survival analysis in PSC, the outcome was defined as liver-transplantation-free survival during the follow-up. Positive PR3-ANCA levels were found in 74/309 (24.0%) of patients with PSC. No BDs and one patient with PBC demonstrated PR3-ANCA positivity. PR3-ANCAs were revealed as independent predictors for a poor PSC outcome (study endpoint: liver transplantation/death, log-rank test, p = 0.02). PR3-ANCA positivity, lower albumin levels, and higher bilirubin concentrations were independent risks of a poor survival (Cox proportional-hazards regression analysis, p < 0.05). The Mayo risk score for PSC was associated with PR3-ANCA positivity (p = 0.01) and the disease severity assessed with a model of end-stage liver disease (MELD) and extended MELD-Na (p < 0.05). PR3-ANCAs detected by a third-generation ELISA are diagnostic and prognostic markers for PSC. Their wider use could help to identify patients who are at-risk of a more severe disease. KW - primary sclerosing cholangitis KW - antibodies against serine protease proteinase-3 KW - survival KW - health-related quality of life KW - liver biochemistry KW - disease severity Y1 - 2022 U6 - https://doi.org/10.3390/diagnostics12112682 SN - 2075-4418 VL - 12 IS - 11 ER - TY - GEN A1 - Khan, Muhammad Moman A1 - Sidorczuk, Katarzyna A1 - Becker, Juliane A1 - Aleksandrowicz, Adrianna A1 - Baraniewicz, Karolina A1 - Ludwig, Christina A1 - Ali, Aamir A1 - Kingsley, Robert A. A1 - Schierack, Peter A1 - Kolenda, Rafał T1 - Characterization of clumpy adhesion of Escherichia coli to human cells and associated factors influencing antibiotic sensitivity T2 - Microbiology Spectrum N2 - Escherichia coli intestinal infection pathotypes are characterized by distinct adhesion patterns, including the recently described clumpy adhesion phenotype. Here, we identify and characterize the genetic factors contributing to the clumpy adhesion of E. coli strain 4972. In this strain, the transcriptome and proteome of adhered bacteria were found to be distinct from planktonic bacteria in the supernatant. A total of 622 genes in the transcriptome were differentially expressed in bacteria present in clumps relative to the planktonic bacteria. Seven genes targeted for disruption had variable distribution in different pathotypes and nonpathogenic E. coli, with the pilV and spnT genes being the least frequent or absent from most groups. Deletion (Δ) of five differentially expressed genes, flgH, ffp, pilV, spnT, and yggT, affected motility, adhesion, or antibiotic stress. ΔflgH exhibited 80% decrease and ΔyggT depicted 184% increase in adhesion, and upon complementation, adhesion was significantly reduced to 13%. ΔflgH lost motility and was regenerated when complemented, whereas Δffp had significantly increased motility, and reintroduction of the same gene reduced it to the wild-type level. The clumps produced by Δffp and ΔspnT were more resistant and protected the bacteria, with ΔspnT showing the best clump formation in terms of ampicillin stress protection. ΔyggT had the lowest tolerance to gentamicin, where the antibiotic stress completely eliminated the bacteria. Overall, we were able to investigate the influence of clump formation on cell surface adhesion and antimicrobial tolerance, with the contribution of several factors crucial to clump formation on susceptibility to the selected antibiotics. KW - Infectious Diseases KW - Cell Biology KW - Microbiology (medical) KW - Genetics KW - General Immunology and Microbiology KW - Ecology KW - Physiology Y1 - 2024 U6 - https://doi.org/10.1128/spectrum.02606-23 SN - 2165-0497 ER - TY - GEN A1 - Azam, Hafiz Muhammad Husnain A1 - Rößling, Rosa Ilse A1 - Geithe, Christiane A1 - Khan, Muhammad Moman A1 - Dinter, Franziska A1 - Hanack, Katja A1 - Prüß, Harald A1 - Husse, Britta A1 - Roggenbuck, Dirk A1 - Schierack, Peter A1 - Rödiger, Stefan T1 - MicroRNA biomarkers as next-generation diagnostic tools for neurodegenerative diseases: a comprehensive review T2 - Frontiers in Molecular Neuroscience N2 - Neurodegenerative diseases (NDs) are characterized by abnormalities within neurons of the brain or spinal cord that gradually lose function, eventually leading to cell death. Upon examination of affected tissue, pathological changes reveal a loss of synapses, misfolded proteins, and activation of immune cells—all indicative of disease progression—before severe clinical symptoms become apparent. Early detection of NDs is crucial for potentially administering targeted medications that may delay disease advancement. Given their complex pathophysiological features and diverse clinical symptoms, there is a pressing need for sensitive and effective diagnostic methods for NDs. Biomarkers such as microRNAs (miRNAs) have been identified as potential tools for detecting these diseases. We explore the pivotal role of miRNAs in the context of NDs, focusing on Alzheimer’s disease, Parkinson’s disease, Multiple sclerosis, Huntington’s disease, and Amyotrophic Lateral Sclerosis. The review delves into the intricate relationship between aging and NDs, highlighting structural and functional alterations in the aging brain and their implications for disease development. It elucidates how miRNAs and RNA-binding proteins are implicated in the pathogenesis of NDs and underscores the importance of investigating their expression and function in aging. Significantly, miRNAs exert substantial influence on post-translational modifications (PTMs), impacting not just the nervous system but a wide array of tissues and cell types as well. Specific miRNAs have been found to target proteins involved in ubiquitination or de-ubiquitination processes, which play a significant role in regulating protein function and stability. We discuss the link between miRNA, PTM, and NDs. Additionally, the review discusses the significance of miRNAs as biomarkers for early disease detection, offering insights into diagnostic strategies. KW - neurodegenerative diseases KW - microRNA KW - biomarkers KW - nervous system KW - diagnostic tools KW - therapeutic tools KW - protein post-translational modifications KW - limitations Y1 - 2024 U6 - https://doi.org/10.3389/fnmol.2024.1386735 SN - 1662-5099 VL - 17 PB - Frontiers Media S.A. ER - TY - GEN A1 - Frost, Fabian A1 - Weiss, Stefan A1 - Hertel, Johannes A1 - Rühlemann, Malte A1 - Bang, Corinna A1 - Franke, Andre A1 - Nauck, Matthias A1 - Dörr, Marcus A1 - Völzke, Henry A1 - Roggenbuck, Dirk A1 - Schierack, Peter A1 - Völker, Uwe A1 - Homuth, Georg A1 - Aghdassi, Ali A. A1 - Sendler, Matthias A1 - Lerch, Markus M. A1 - Weiss, Frank U. T1 - Fecal glycoprotein 2 is a marker of gut microbiota dysbiosis and systemic inflammation T2 - Gut Pathogens N2 - Background autoantigenic glycoprotein 2 (GP2) is an important component of the innate immune system which originates from the exocrine pancreas as well as from the small intestines. The relationship of GP2 with the intestinal microbiome as well as the systemic implications of increased fecal GP2 levels are, however, still unclear. Therefore, fecal samples from 2,812 individuals of the Study of Health in Pomerania (SHIP) were collected to determine GP2 levels (enzyme-linked immunosorbent assay) and gut microbiota profiles (16 S rRNA gene sequencing). These data were correlated and associated with highly standardised and comprehensive phenotypic data of the study participants. Results Fecal GP2 levels were increased in individuals with higher body mass index and smokers, whereas lower levels were found in case of preserved exocrine pancreatic function, female sex or a healthier diet. Moreover, higher GP2 levels were associated with increased serum levels of high-sensitivity C-reactive protein, loss of gut microbial diversity and an increase of potentially detrimental bacteria (Streptococcus, Haemophilus, Clostridium XIVa, or Collinsella). At the same time, predicted microbial pathways for the biosynthesis of beneficial short-chain fatty acids or lactic acid were depleted in individuals with high fecal GP2. Of note, GP2 exhibited a stronger association to overall microbiome variation than calprotectin. Conclusion Fecal GP2 is a biomarker of gut microbiota dysbiosis and associated with increased systemic inflammation. The intestines may be more important as origin for GP2 than pancreatic acinar cells. Future studies need to investigate the potential clinical value in disease specific patient cohorts. Y1 - 2024 U6 - https://doi.org/10.1186/s13099-024-00657-1 SN - 1757-4749 VL - 16 IS - 1 SP - 1 EP - 11 PB - Springer Science and Business Media LLC ER - TY - GEN A1 - Sidorczuk, Katarzyna A1 - Burdukiewicz, Michał A1 - Cerk, Klara A1 - Fritscher, Joachim A1 - Kingsley, Robert A. A1 - Schierack, Peter A1 - Hildebrand, Falk A1 - Kolenda, Rafał T1 - AdhesiomeR: a tool for Escherichia coli adhesin classification and analysis T2 - BMC Genomics N2 - AbstractAdhesins are crucial factors in the virulence of bacterial pathogens such as Escherichia coli. However, to date no resources have been dedicated to the detailed analysis of E. coli adhesins. Here, we provide adhesiomeR software that enables characterization of the complete adhesin repertoire, termed the adhesiome. AdhesiomeR incorporates the most comprehensive database of E. coli adhesins and facilitates an extensive analysis of adhesiome. We demonstrate that adhesiomeR achieves 98% accuracy when compared with experimental analyses. Based on analysis of 15,000 E. coli genomes, we define novel adhesiome profiles and clusters, providing a nomenclature for a unified comparison of E. coli adhesiomes. Y1 - 2024 U6 - https://doi.org/10.1186/s12864-024-10525-6 SN - 1471-2164 VL - 25 IS - 1 SP - 1 EP - 10 PB - Springer Science and Business Media LLC ER - TY - GEN A1 - Kittl, Sonja A1 - Frey, Caroline F. A1 - Brodard, Isabelle A1 - Scalisi, Nadia A1 - Vargas Amado, Maria Elena A1 - Thomann, Andreas A1 - Schierack, Peter A1 - Jores, Joerg T1 - Zoonotic bacterial and parasitic intestinal pathogens in foxes, raccoons and other predators from eastern Germany T2 - Environmental Microbiology Reports N2 - AbstractIn this study, we investigated faecal specimens from legally hunted and road‐killed red foxes, raccoons, raccoon dogs, badgers and martens in Germany for parasites and selected zoonotic bacteria. We found that Baylisascaris procyonis, a zoonotic parasite of raccoons, had spread to northeastern Germany, an area previously presumed to be free of this parasite. We detected various pathogenic bacterial species from the genera Listeria, Clostridium (including baratii), Yersinia and Salmonella, which were analysed using whole‐genome sequencing. One isolate of Yersinia enterocolitica contained a virulence plasmid. The Salmonella Cholerasuis isolate encoded an aminoglycoside resistance gene and a parC point mutation, conferring resistance to ciprofloxacin. We also found tetracycline resistance genes in Paeniclostridium sordellii and Clostridium baratii. Phylogenetic analyses revealed that the isolates were polyclonal, indicating the absence of specific wildlife‐adapted clones. Predators, which scavenge from various sources including human settlements, acquire and spread zoonotic pathogens. Therefore, their role should not be overlooked in the One Health context. Y1 - 2024 U6 - https://doi.org/10.1111/1758-2229.13261 SN - 1758-2229 VL - 16 IS - 3 SP - 1 EP - 7 PB - Wiley ER - TY - GEN A1 - Kuhnert, Peter A1 - Brodard, Isabelle A1 - Ackermann, Stefanie A1 - Schierack, Peter A1 - Jores, Joerg A1 - Heliyon, J. T1 - Serological and molecular detection as well as typing of Leptospira spp. in foxes, raccoons, and other wild carnivores in North-Eastern Germany, 2021–2022 T2 - Heliyon Y1 - 2024 U6 - https://doi.org/10.1016/j.heliyon.2023.e23268 SN - 2405-8440 VL - 10 IS - 1 SP - 1 EP - 6 PB - Elsevier BV ER - TY - GEN A1 - Chilimoniuk, Jarosław A1 - Erol, Anna A1 - Rödiger, Stefan A1 - Burdukiewicz, Michał T1 - Challenges and opportunities in processing NanoString nCounter data T2 - Computational and Structural Biotechnology Journal Y1 - 2024 U6 - https://doi.org/10.1016/j.csbj.2024.04.061 SN - 2001-0370 VL - 23 SP - 1951 EP - 1958 PB - Elsevier BV ER - TY - GEN A1 - Lopens, Steffi A1 - Schierack, Peter A1 - Krause, Jenny A1 - Piaszczyński, Michał A1 - Król, Robert A1 - Staroń, Robert A1 - Krupa, Łukasz A1 - Gutkowski, Krzysztof A1 - Kruk, Beata A1 - Grąt, Michał A1 - Krawczyk, Marek A1 - Patkowski, Waldemar A1 - Glaser, Fabian A1 - Rödiger, Stefan A1 - Grossmann, Kai A1 - Pająk, Jacek A1 - Milkiewicz, Piotr A1 - Lammert, Frank A1 - Zieniewicz, Krzysztof A1 - Schramm, Christoph A1 - Roggenbuck, Dirk A1 - Krawczyk, Marcin T1 - Antimicrobial glycoprotein 2 (GP2) in gallstones, bile fluid and peribiliary glands of patients with primary sclerosing cholangitis T2 - Clinica Chimica Acta Y1 - 2024 U6 - https://doi.org/10.1016/j.cca.2024.119841 SN - 0009-8981 VL - 562 SP - 1 EP - 8 PB - Elsevier BV ER - TY - GEN A1 - Geithe, Christiane A1 - Zeng, Bo A1 - Schmidt, Carsten A1 - Dinter, Franziska A1 - Roggenbuck, Dirk A1 - Lehmann, Werner A1 - Dame, Gregory A1 - Schierack, Peter A1 - Hanack, Katja A1 - Rödiger, Stefan T1 - A multiplex microchamber diffusion assay for the antibody-based detection of microRNAs on randomly ordered microbeads T2 - Biosensors and Bioelectronics: X Y1 - 2024 U6 - https://doi.org/10.1016/j.biosx.2024.100484 SN - 2590-1370 VL - 18 (2024) SP - 1 EP - 7 PB - Elsevier BV ER - TY - GEN A1 - Rödiger, Stefan A1 - Kögler, Martin A1 - Birkholz, Mario T1 - Open-Source software, fediverse and custom ROMs as tools for a sustainable internet T2 - 2024 Electronics Goes Green 2024+ (EGG) Y1 - 2024 UR - https://ieeexplore.ieee.org/document/10631179 U6 - https://doi.org/10.23919/EGG62010.2024.10631179 SP - 1 EP - 13 PB - IEEE CY - Berlin ER - TY - GEN A1 - Brauckhoff, Tim A1 - Rödiger, Stefan T1 - BiopixR - extended vignette for bioimage analysis package in R Y1 - 2024 U6 - https://doi.org/10.5281/zenodo.12744223 ER - TY - GEN A1 - Naz, Fizza A1 - Ahmad, Abrar A1 - Sarwar, Yasra A1 - Khan, Muhammad Moman A1 - Schierack, Peter A1 - Rauf, Waqar A1 - Ali, Aamir T1 - Characterization of Salmonella enterica Biofilms and Antibiofilm Effect of Carvacrol and 2-Aminobenzimidazole T2 - Foodborne Pathogens and Disease Y1 - 2024 U6 - https://doi.org/10.1089/fpd.2023.0044 SN - 1535-3141 VL - 21 IS - 1 SP - 52 EP - 60 PB - Mary Ann Liebert Inc. ER - TY - GEN A1 - Khan, Muhammad Moman A1 - Mushtaq, Muhammad Ahmed A1 - Abbas, Nayyar A1 - Fatima, Fariha A1 - Gibbon, Marjorie J. A1 - Schierack, Peter A1 - Mohsin, Mashkoor T1 - Occurrence, antimicrobial resistance and genomic features of Klebsiella pneumoniae from broiler chicken in Faisalabad, Pakistan T2 - Frontiers in Veterinary Science N2 - IntroductionThe dissemination of antimicrobial resistance (AMR) in critical priority pathogens is a significant threat. Non-clinical reservoirs of AMR, such as agriculture and food production facilities, may contribute to the transmission of clinically relevant pathogens such as multidrug-resistant (MDR) Klebsiella pneumoniae. There is currently very limited knowledge regarding the population structure and genomic diversity of K. pneumoniae in poultry production in Pakistan.MethodsWe explored healthy broilers in a commercial farm from Faisalabad, Pakistan, and identified six K. pneumoniae strains from 100 broiler birds. We characterized the strains, determining clonality, virulence and antimicrobial resistance genes using next generation sequencing.ResultsThe evaluation of antimicrobial susceptibility revealed that all the strains were MDR. Genomic analysis showed that 3/6 strains belonged to ST152, harbouring acquired resistance aminoglycosides [aadA2, aph(4′)-Ia], β-lactams (blaSHV-187, blaLAP2), fosfomycin (fosA6), tetracycline (tetA), trimethoprim (dfrA12), quinolone (qnrS1), sulphonamides (sul2) and phenicol (floR). All the strains harboured the efflux pump genes oqxA, oqxB, emrR, kpnG, kpnH, kpnF, baeR, mtdB and mtdC. All six strains encoded identical virulence profiles possessing six genes, i.e., ureA, iutA, entB, allS, fimH and mrkD. Phylogenomic analysis of the dominant sequence type (ST152) present in our dataset with publicly available genomes showed that the isolates clustered to strains mainly from human sources and could pose a potential threat to food safety and public health.DiscussionThe combination of these findings with antimicrobial use data would allow a better understanding of the selective pressures that may be driving the spread of AMR. This is the first report of MDR K. pneumoniae isolated from broiler hens in Pakistan, and the finding suggests that routine surveillance of WHO critical priority pathogens in such settings would be beneficial to the development of effective control strategies to reduce AMR. KW - antimicrobial resistance KW - poultry KW - genomics KW - sequence type Y1 - 2024 U6 - https://doi.org/10.3389/fvets.2024.1433124 SN - 2297-1769 VL - 11 PB - Frontiers Media S.A. ER - TY - GEN A1 - Brauckhoff, Tim A1 - Kieffer, Coline A1 - Rödiger, Stefan T1 - Extracting Insights from biological images T2 - Journal of Open Source Software Y1 - 2024 U6 - https://doi.org/10.21105/joss.07074 SN - 2475-9066 VL - 9 IS - 102 ER - TY - GEN A1 - Liedtke, Victoria A1 - Rose, Laura A1 - Hiemann, Rico A1 - Nasser, Abdullah A1 - Rödiger, Stefan A1 - Bonaventura, Alena A1 - Winkler, Laura A1 - Sowa, Mandy A1 - Stöckle, Michael A1 - Schierack, Peter A1 - Junker, Kerstin A1 - Roggenbuck, Dirk T1 - Over-Expression of LEDGF/p75 in HEp-2 Cells Enhances Autoimmune IgG Response in Patients with Benign Prostatic Hyperplasia—A Novel Diagnostic Approach with Therapeutic Consequence? T2 - International Journal of Molecular Sciences N2 - Lens epithelium-derived growth factor splice variant of 75 kDa (LEDGF/p75) is an autoantigen over-expressed in solid tumors and acts as a stress-related transcriptional co-activator. Participation of autoimmune responses in the pathophysiology of benign prostatic hyperplasia (PBH) and a corresponding immunosuppressive therapy by TNFalpha antagonists has been recently suggested. Thus, autoAb testing could aid in the diagnosis of BPH patients profiting from such therapy. We generated CRISPR/Cas9 modified HEp-2 LEDGF knock-out (KO) and HEp-2 LEDGF/p75 over-expressing (OE) cells and examined IgG autoantibody reactivity to LEDGF/p75 in patients with prostate cancer (PCa, n = 89), bladder cancer (BCa, n = 116), benign prostatic hyperplasia (BPH, n = 103), and blood donors (BD, n = 60) by indirect immunofluorescence assay (IFA). Surprisingly, we could not detect elevated binding of autoAbs against LEDGF/p75 in cancer patients, but autoAb reactivity to LEDGF/p75 OE cells in about 50% of patients with BPH was unexpectedly significantly increased. Furthermore, a line immunoassay enabling the detection of 18 different autoAbs revealed a significantly increased occurrence of anti-dsDNA autoAbs in 34% of BPH patients in contrast to tumor patients and BD. This finding was confirmed by anti-mitochondrial (mDNA) autoAb detection with the Crithidia luciliae immunofluorescence test, which also showed a significantly higher prevalence (34%) of anti-mDNA autoAbs in BPH. In summary, our study provided further evidence for the occurrence of autoimmune responses in BPH. Furthermore, LEDGF/p75 over-expression renders HEp-2 cells more autoantigenic and an ideal target for autoAb analysis in BPH with a potential therapy consequence. KW - LEDGF/p75 KW - autoimmunity KW - CRISPR/Cas9 KW - dsDNA KW - mDNA Y1 - 2023 U6 - https://doi.org/10.3390/ijms24076166 SN - 1422-0067 VL - 24 IS - 7 ER - TY - GEN A1 - Khan, Muhammad Moman A1 - Ali, Aamir A1 - Kolenda, Rafał A1 - Olowe, Olugbenga Adekunle A1 - Weinreich, Jörg A1 - Li, Ganwu A1 - Schierack, Peter T1 - The role of AJB35136 and fdtA genes in biofilm formation by avian pathogenic Escherichia coli T2 - BMC Veterinary Research Y1 - 2023 U6 - https://doi.org/10.1186/s12917-023-03672-7 SN - 1746-6148 VL - 19 ER - TY - BOOK ED - Reghunadhan, Arunima ED - H, Akhina ED - Thomas, Sabu T1 - Applications of biopolymers in science, biotechnology, and engineering Y1 - 2024 SN - 9781119783442 U6 - https://doi.org/10.1002/9781119783473 PB - Wiley ET - 1 ER - TY - CHAP A1 - Mumtaz, Mehvish A1 - Hussain, Nazim A1 - Salam, Sidra A1 - Azam, Hafiz Muhammad Husnain A1 - Łukasik, Natalia A1 - Kim, Tak H. A1 - Santos, José Cleiton Sousa dos A1 - Schmidt, Jens Ejbye T1 - Chitosan-based nanomaterials for removal of water pollutants T2 - Chitosan-Based Hybrid Nanomaterials Y1 - 2024 SN - 9780443218910 U6 - https://doi.org/10.1016/B978-0-443-21891-0.00016-0 SP - 291 EP - 318 PB - Elsevier ER - TY - GEN A1 - Roei, Tulchinsky A1 - Boris, Gilburd A1 - Yehuda, Shovman A1 - Milena, Tocut A1 - Eleanor, Zeruya A1 - Ariel, Binyaminov A1 - Tima, Davidson A1 - Büttner, Thomas A1 - Nasser, Abdullah A1 - Michel, Juliane A1 - Rödiger, Stefan A1 - Schierack, Peter A1 - Howard, Amital A1 - Roggenbuck, Dirk A1 - Yehuda, Shoenfeld A1 - Ora, Shovman T1 - CytoBead ANA 2 assay : a novel method for the detection of antinuclear antibodies T2 - Scientific reports N2 - Detection of anti-nuclear autoantibodies (ANA) is based on a two-step algorithm including indirect immunofluorescence (IIF) on HEp2 cells and subsequent reflex/confirmatory testing for specific autoantibodies. Simultaneous cell- and microbead-based autoantibody detection by IIF may be utilized for the evaluation of systemic autoimmune rheumatic diseases (SARDs). In the present study, we assessed the performance of CytoBead ANA 2 in the detection of ANA and ANA-specific autoantibodies, compared to ANA IIF and BioPlex™ 2200. We also tested the ability of CytoBead ANA DFS-70 to identify dense-fine speckled (DFS) pattern associated with anti-DFS70 antibodies in non-SARDs patients. Hundred-twelve routine sera samples were assessed by manual CytoBead ANA 2 for the presence of ANA and specific autoantibodies. In parallel, these samples were analyzed by HEp2 ANA IIF test and a subsequent multiplexed assay BioPlex™ 2200 ANA. Twenty-nine ANA-positive samples obtained from non-SARDs patients and exhibiting DFS pattern by ANA IIF were further tested by CytoBead ANA DFS-70. A substantial agreement was observed between classical ANA IIF and manual CytoBead ANA 2 for the detection of ANA (k = 0.74). Discordant results were mainly associated with the presence of anti-SSA/Ro antibodies detected by CytoBead ANA 2 in ANA IIF negative patients. A good to almost perfect agreement was found between CytoBead ANA 2 and BioPlex™ 2200 for detection of specific antibodies with kappa values ranging from 0.70 to 0.90. Twenty samples (68.9%) obtained from 29 ANA IIF positive without SARDs patients exhibited DFS pattern in CytoBead ANA DFS-70, which confirmed the presence of anti-DFS70 antibodies. The diagnostic performance of manual CytoBead ANA 2 for ANA screening and detection of ANA specific antibodies is comparable to the diagnostic performance of ANA IIF followed by BioPlex™ 2200. This novel one-step assay enables simultaneous ANA screening and confirmation and represents a promising alternative approach to the time-consuming and costly two-tier ANA analysis. KW - Anti-nuclear antibodies KW - Anti-DFS70 antibodies KW - Indirect immunofluorescence KW - Multiplexed assay KW - CytoBead technology Y1 - 2025 U6 - https://doi.org/10.1038/s41598-025-04583-3 SN - 2045-2322 VL - 15 IS - 1 SP - 1 EP - 11 PB - Nature Publishing Group UK CY - London ER - TY - GEN A1 - Azam, Hafiz Muhammad Husnain A1 - Mumtaz, Mehvish A1 - Rödiger, Stefan A1 - Schierack, Peter A1 - Hussain, Nazim A1 - Aisha, Ambreen T1 - MicroRNAs in neurodegenerative diseases : from molecular mechanisms to clinical biomarkers, detection methods and therapeutic strategies—advances and challenges T2 - Neurological sciences N2 - Neurodegenerative diseases (NDDs) pose significant challenges in early detection and treatment due to their complex pathophysiology and heterogeneous clinical presentations. MicroRNAs (miRNAs), small noncoding RNAs that regulate gene expression, have emerged as promising diagnostic biomarkers and therapeutic targets in NDDs. Pathological examination of affected tissues reveals early synaptic dysfunction, protein misfolding, and neuroinflammation occur prior to overt clinical symptoms, highlighting the importance of sensitive diagnostics approaches in prodromal stages. This review summarizes for researchers on the role of miRNAs in NDDs by examining their diagnostic potential in biofluids such as blood and cerebrospinal fluid, and their therapeutic applicability through inhibition or replacement strategies. Literature from peer-reviewed databases was assessed with a focus on recent advances in molecular detection platforms, computational modeling of miRNA–mRNA interactions, and preclinical/clinical investigations. More than 2600 human miRNAs have been identified, collectively regulating over half of mammalian protein-coding genes. Quantitative methodologies, particularly reverse transcription quantitative PCR (RT-qPCR), enable reliable miRNA profiling, facilitating early diagnosis and prognosis of NDDs. Therapeutic strategies, including antagomirs, mimics, sponges and viral or non-viral delivery systems, show promise in modulating disease pathways. However, significant challenges remain, including variability in miRNA extraction and quantification protocols, off-target effects, delivery barriers across the blood brain barrier and limited reproducibility across studies. MiRNAs represent a class of molecular tools with potential to transform diagnostics and therapeutics in NDDs. Future research should prioritize methodological standardization, validation in large multicenter cohorts, and improved computational approaches to elucidate miRNA-mediated regulatory networks in NDDs. Replication studies and translational research are essential harnessing the the full clinical utility of miRNAs in the management of Alzheimer disease, Parkinson disease and other NDDs. KW - MicroRNA KW - Neurodegenerative diseases KW - Alzheimer’s disease KW - Parkinson’s disease KW - Huntington’s disease KW - Amyotrophic lateral sclerosis KW - Diagnostic biomarkers KW - MiRNA-based therapeutics KW - Molecular diagnostics KW - Gene regulation KW - Biofluid biomarkers KW - Delivery technologies KW - Neuroinflammation KW - RT-qPCR KW - NGS KW - Clinical translation Y1 - 2025 U6 - https://doi.org/10.1007/s10072-025-08419-w SN - 1590-3478 PB - Springer CY - Milano ER -