TY - CHAP A1 - Pohlert, M. A1 - Martienssen, Marion A1 - Weiß, H. A1 - Trabitzsch, R. A1 - Geistlinger, Helmut T1 - SF6-Gastracerversuche zur Abschätzung der Raumwirkung bei Direktgasinjektionen zur Grundwassersanierung T2 - Behandlungstechnologien für bergbaubeeinflusste Wässer, Proceedingsband zu den Workshops am Geologischen Institut der TU Bergakademie Freiberg am 12. und 13. Juni 2008 Y1 - 2008 SP - 1141 EP - 1148 PB - Institut für Geologie CY - Freiberg ER - TY - GEN A1 - Koch, Roland A1 - Straub, Andrea A1 - Mangold, Sylke A1 - Schöpke, Ralph A1 - Martienssen, Marion T1 - Tausalz, Temperatur und chemische P-Fällung T2 - Wasserwirtschaft, Wassertechnik : wwt Y1 - 2008 SN - 1438-5716 IS - 11/12 SP - 41 EP - 46 ER - TY - GEN A1 - Leschik, Sebastian A1 - Musolff, Andreas A1 - Martienssen, Marion A1 - Krieg, Ronald A1 - Bayer-Raich, Marti A1 - Reinstorf, Frido A1 - Strauch, Gerhard A1 - Schirmer, Mario T1 - Investigation of sewer exfiltration using integral pumping tests and wastewater indicators Y1 - 2009 U6 - https://doi.org/10.1016/j.jconhyd.2009.10.001 ER - TY - GEN A1 - Leschik, Sebastian A1 - Musolff, Andreas A1 - Krieg, Ronald A1 - Martienssen, Marion A1 - Bayer-Raich, Marti A1 - Strauch, Gerhard A1 - Schirmer, Mario A1 - Reinstorf, Frido T1 - Application of integral pumping tests to investigate the influence of a losing stream on groundwater quality Y1 - 2009 U6 - https://doi.org/10.5194/hess-13-1765-2009 ER - TY - GEN A1 - Schmidt, C. A1 - Kalbus, Edda A1 - Martienssen, Marion A1 - Schirmer, M. T1 - The influence of heterogeneous groundwater discharge an the timescales of contaminat mass flux from streambed sediments field evidence and long-term predictions T2 - Hydrology and Earth Systems Sciences Discussion Y1 - 2008 VL - 5 IS - 2 SP - 971 EP - 1001 ER - TY - GEN A1 - Martienssen, Marion A1 - Kukla, S. A1 - Balcke, G. U. A1 - Hasselwander, E. A1 - Schirmer, M. T1 - Evidence of natural occurring MTBE biodegradation by analysing metabolites and biodegradation products Y1 - 2006 ER - TY - GEN A1 - Beckmann, Annika A1 - Gerhardt, Matthias A1 - Zittwitz, Mark A1 - Martienssen, Marion A1 - Krieg, Ronald A1 - Geistlinger, Helmut A1 - Schirmer, Mario T1 - Das Oxywall-Projekt: Anwendung eines Verfahrens zur Direktgasinjektion von Sauerstoff zur in situ Sanierung von organisch kontaminierten Grundwässern T2 - Altlastenspektrum Y1 - 2007 SN - 0942-3818 VL - 16 IS - 8 SP - 153 EP - 159 ER - TY - PAT A1 - Ringpfeil, Manfred A1 - Gerhardt, Matthias A1 - Zittwitz, Mark A1 - Beckmann, Annika A1 - Geistlinger, Helmut A1 - Schirmer, Mario A1 - Martienssen, Marion T1 - Verfahren und Anordnung zur Direktgasinjektion für die Sanierung von kontaminierten Börden und/oder Grundwasserleitern sowie ein entsprechendes Computerprogramm und ein entsprechendes computerlesbares Speichermedium Y1 - 2007 ER - TY - CHAP A1 - Martienssen, Marion T1 - Erste Ergebnisse aus dem F+E-Thema "Methanoxidation in Rekultivierungs-/Wasserhaushaltsschichten in Sachsen-Anhalt T2 - 3. Leipziger Deponiefachtagung, 22/23.02.2007, Leipzig, Conference proceedings Y1 - 2007 PB - HTWK CY - Leipzig ER - TY - GEN A1 - Böckelmann, Uta A1 - Janke, Andrea A1 - Kuhn, Ramona A1 - Neu, Thomas R. A1 - Wecke, Jörg A1 - Lawrence, John R. A1 - Szewzyk, Ulrich T1 - Bacterial extracellular DNA forming a defined network-like structure Y1 - 2006 ER - TY - GEN A1 - Guiot, Serge R. A1 - Kuhn, Ramona A1 - Lévesque, M. J. A1 - Cimpoia, Ruxandra T1 - Ultrastructure of a bioelectrolytic methanogenic/methanotrophic granular biofilm for the complete degradation of tetrachloroethylene in contaminated groundwater Y1 - 2007 ER - TY - GEN A1 - Guiot, Serge R. A1 - Cimpoia, Ruxandra A1 - Kuhn, Ramona A1 - Alaplantive, Aude T1 - Electrolytic Methanogenic-methanotropic coupling for Tetrachloroethylene Bioremediation: A Proof of Concept T2 - Environmental science & technology Y1 - 2008 SN - 0013-936X VL - 42 IS - 8 SP - 3011 EP - 3017 ER - TY - CHAP A1 - Nixdorf, Brigitte A1 - Becker, Katja A1 - Grüneberg, Björn A1 - Böllmann, Jörg A1 - Martienssen, Marion T1 - Stickstoffumsätze in unterschiedlichen Gewässertypen Berlin/Brandenburgs, Teil 2: Erste Ergebnisse zu einträgen und Umsätzen T2 - Erweiterte Zusammenfassungen der Jahrestagung 2011 der Deutschen Gesellschaft für Limnologie (DGL) und der deutschen und österreichischen Sektion der Societas Internationalis Limnologiae (SIL), Weihenstephan, 12. - 16. Sept. 2011 Y1 - 2012 SN - 978-3-9813095-2-2 SP - 239 EP - 244 PB - Eigenverl. der DGL CY - Hardegsen ER - TY - CHAP A1 - Böllmann, Jörg A1 - Martienssen, Marion A1 - Grüneberg, Björn T1 - Vergleich der mikrobiellen Nitratreduktion in Sediment und Pelagial eines eutrophen Flusssees und eines mesotrophen geschichteten Sees T2 - Erweiterte Zusammenfassungen der Jahrestagungder Deutschen Gesellschaft für Limnologie (DGL), Potsdam - Berlin, 09. - 13. September 2013 KW - Nitratreduktion Y1 - 2014 SN - 978-3-9813095-5-3 SP - 35 EP - 39 PB - Hardegsen CY - Eigenverlag der DGL ER - TY - GEN A1 - Göring, Daniel A1 - Martienssen, Marion T1 - Elimination von Epichlorhydrin bei der Behandlung industrieller Abwässer T2 - KA - Korrespondenz Abwasser, Abfall Y1 - 2014 SN - 1866-0029 VL - 61 IS - 5 SP - 447 EP - 455 ER - TY - CHAP A1 - Böllmann, Jörg A1 - Scholler, Markus T1 - Preliminary studies on the spread of the rust fungus Puccinia glechomatis in North America T2 - Joint Meeting of the American Phytopathological Society, the Mycological Society of America, and the Society of Nematologists, Salt Lake City, Utah, USA, August 25-29, 2001 Y1 - 2001 N1 - Phytopathology 21(2001)6 SP - S. 104 ER - TY - CHAP A1 - Böllmann, Jörg A1 - Scholler, Markus T1 - The Life Cycle of Puccinia glechomatis, a microcyclic rust on Glechoma hederacea T2 - 2002 MSA Annual Meeting Corvallis, OREGON Y1 - 2002 UR - http://msafungi.org/wp-content/uploads/Inoculum/53%283%29.pdf N1 - Inoculum : newsletter of the Mycological Society of America 53(2002)3 SP - S. 21 ER - TY - GEN A1 - Rathsack, Kristina A1 - Böllmann, Jörg A1 - Martienssen, Marion T1 - Comparative Study of Different Methods for Analyzing Denitrifying Bacteria in Fresh Water Ecosystems T2 - Journal of Water Resource and Protection N2 - Bacteria capable of denitrification play a significant role in the nitrogen cycle of freshwater ecosystems. By metabolizing nitrogen compounds they e.g. counteract the eutrophication of natural waters. To get detailed insights into the in situ turnover rates of nitrogen a reliable tool of quantification for active microorganisms is essential. In the present investigation, quantification capabilities of a molecular tool (Polymerase Chain Reaction—PCR) and a cultivation based tool (Most probable number—MPN) were investigated and compared. The total bacterial concentration yielded by the molecular PCR approach was up to 6-fold higher compared to the results of the MPN approach. However, the portion of culturable denitrifying bacteria compared to the number of specific gene copies (nirS) was much lower. Depending on the environmental conditions, the difference between the PCR and the MPN approach was up to three orders of magnitude. From lab scale experiments with a pure P. aeroginosa strain it can be concludes, that these differences are not the result of inappropriate culture conditions but rather reflect the portion of so called viable but not culturable bacteria (VBNC). Low nitrate concentrations as found in many fresh water ecosystems induced a significant increase in the portion of non culturable denitrifying bacteria. Referred to the investigation of dynamic populations, the number of metabolic active bacteria is represented by the MPN rather than by the PCR approach. Y1 - 2014 SN - 1945-3094 SN - 1945-3108 VL - 6 IS - 6 SP - 609 EP - 617 ER - TY - GEN A1 - Rathsack, Kristina T1 - Unerwünschte Bewohner : Wasserasseln und andere Makroorganismen in Trinkwasserversorgungsnetzen T2 - IKZ Haustechnik : Magazin für Gebäude- und Energietechnik, Sonderheft Trinkwasserhygiene 2013 Y1 - 2013 IS - Sonderheft ER - TY - GEN A1 - Böllmann, Jörg A1 - Schöller, Markus T1 - Life cycle and life strategy features of Puccinia glechomatis (Uredinales) favorable for extending the natural range of distribution T2 - Mycoscience Y1 - 2006 U6 - https://doi.org/10.1007/s10267-006-0282-z SN - 1340-3540 VL - 47 IS - 3 SP - 152 EP - 158 ER - TY - GEN A1 - Rathsack, Kristina A1 - Rathsack, Udo T1 - Besiedlung von Wasserversorgungssystemen durch Asseln und andere Invertebraten : Weiterentwickeltes Modell und Messsystem biologischer Prozesse T2 - GWF : Wasser, Abwasser Y1 - 2010 SN - 0016-3651 VL - 151 IS - 2 SP - 200 EP - 207 ER - TY - GEN A1 - Rathsack, Kristina A1 - Rathsack, Udo T1 - Besiedlung von Wasserversorgungssystemen durch Asseln und andere Invertebraten T2 - GWF : Wasser, Abwasser Y1 - 2009 SN - 0016-3651 VL - 150 IS - 6 SP - 451 EP - 458 ER - TY - GEN A1 - Rathsack, Kristina A1 - Stackebrandt, E. A1 - Reitner, Joachim A1 - Schumann, G. T1 - Microorganisms Isolated from Deep Sea Low-temperature Influenced Oceanic Crust Basalts and Sediment Samples Collected along the Mid-Atlantic Ridge T2 - Geomicrobiology journal Y1 - 2009 SN - 0149-0451 VL - 26 IS - 4 SP - 264 EP - 274 ER - TY - GEN A1 - Nayernia, Karim A1 - Nolte, Jessica A1 - Michelmann, Hans W. A1 - Lee, Jae Ho A1 - Rathsack, Kristina A1 - Drusenheimer, Nadja A1 - Dev, Arvind A1 - Wulf, Gerald A1 - Ehrmann, Ingrid E. A1 - Elliott, David J. A1 - Okpanyi, Vera A1 - Zechner, Ulrich A1 - Haaf, Thomas A1 - Meinhardt, Andreas A1 - Engel, Wolfgang T1 - In Vitro-Differentiated Embryonic Stem Cells Give Rise to Male Gametes that Can Generate Offspring Mice T2 - Developmental Cell Y1 - 2006 SN - 1534-5807 SN - 1878-1551 VL - 11 IS - 1 SP - 125 EP - 132 ER - TY - THES A1 - Rathsack, Kristina T1 - Microbial diversity within the low-temperature influenced deep marine biosphere along the Mid-Atlantic-Ridge Y1 - 2010 ER - TY - GEN A1 - Nolte, Jessica A1 - Rathsack, Kristina A1 - Drusenheimer, Nadja A1 - Dev, Arvind A1 - Zechner, Ulrich A1 - Haaf, Thomas A1 - Meinhardt, Andreas A1 - Michelmann, Hans W. A1 - Nayernia, Karim A1 - Engel, Wolfgang T1 - Männliche Keimzellen aus embryonalen Stammzellen T2 - Medizinische Genetik Y1 - 2007 SN - 0936-5931 VL - 19 IS - 1 SP - 10 EP - 17 ER - TY - CHAP A1 - Böllmann, Jörg T1 - Die Bedeutung von Anthropochorie und die Verbreitung von infiziertem Wirtsgewebe am Beispiel des Rostpilzes Puccinia glechomatis T2 - Treffpunkt biologische Vielfalt, aktuelle Forschung im Rahmen des Übereinkommens über die biologische Vielfalt vorgestellt auf einer wissenschaftlichen Expertentagung an der Internationalen Naturschutzakademie Insel Vilm vom 21. - 25. August 2006 Y1 - 2007 UR - http://www.bfn.de/fileadmin/MDB/documents/service/skript207.pdf SP - 107 EP - 112 PB - Bundesamt für Naturschutz CY - Bonn [u.a.] ER - TY - CHAP A1 - Scholler, Markus A1 - Böllmann, Jörg T1 - Puccinia glechomatis and P. lagenophorae in North America: invasion patterns, life cycle and life strategies T2 - Mycological Society of America (MSA) annual meeting, Hilo (Hawaii), 2006 Y1 - 2006 UR - http://msafungi.org/wp-content/uploads/Inoculum/56%284%29.pdf N1 - Inoculum : newsletter of the Mycological Society of America 56(2006)4 ER - TY - CHAP A1 - Geistlinger, Helmut A1 - Beckmann, Annika A1 - Gerhardt, Matthias A1 - Martienssen, Marion A1 - Schirmer, Mario T1 - Das Feldexperiment OXYWALL: Direktgasinjektion von Sauerstoff zur in situ Sanierung von organisch kontaminierten Grundwässern T2 - Behandlungstechnologien für bergbaubeeinflusste Wässer, GIS - Geowissenschaftliche Anwendungen und Entwicklungen, Proceedingsband zu den Workshops am Geologischen Institut der TU Bergakademie Freiberg am 14. und 15. Juni 2007 Y1 - 2007 SP - 99 EP - 106 PB - Inst. für Geologie CY - Freiberg ER - TY - THES A1 - Fichtner, Nicole T1 - Untersuchung zur Leistungsfähigkeit von Biofilmverfahren mit frei beweglichen Aufwuchskörpern bei der kommunalen Abwasserbehandlung Y1 - 2015 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:co1-opus4-34765 CY - Cottbus ER - TY - CHAP A1 - Geistlinger, Helmut A1 - Lazik, D. A1 - Eisemann, D. A1 - Beckmann, Annika A1 - Martienssen, Marion A1 - Schirmer, Mario A1 - Zittwitz, Mark A1 - Gerhardt, Matthias T1 - Reaktive Gaswände mittels Direktgasinjektion: Vom Bench-Scale zum Field-Scale (OXYWALL) T2 - Behandlungstechnologien für bergbaubeeinflusste Wässer, GIS - geowissenschaftliche Anwendungen und Entwicklungen, Proceedingsband zu den Workshops am Geologischen Institut der TU Bergakademie Freiberg am 22. und 23. Juni 2006 Y1 - 2006 SP - 159 EP - 163 PB - Inst. für Geologie CY - Freiberg ER - TY - PAT A1 - Ringpfeil, Manfred A1 - Gerhardt, Matthias A1 - Zittwitz, Mark A1 - Beckmann, Annika A1 - Geistlinger, Helmut A1 - Schirmer, Mario A1 - Martienssen, Marion T1 - Verfahren und Anordnung zur Direktgasinjektion für die Sanierung von kontaminierten Böden und /oder Grundwasserleitern sowie ein entsprechendes Computerprogramm und ein entsprechendes computerlesbares Speichermedium Y1 - 2007 ER - TY - CHAP A1 - Martienssen, Marion A1 - König, W. T1 - Online-Bestimmung von Nitrifikationsinhibitoren in biologischen Kläranlagen T2 - Technische Systeme für Biotechnologie und Umwelt, 11. Heiligenstädter Kolloquium, Heilbad Heiligenstadt, 30.09. - 02.10.2002 Y1 - 2003 SN - 3-00-011287-1 SP - 205 EP - 210 PB - Institut für Bioprozess- und Analysenmesstechnik CY - Heiligenstadt ER - TY - GEN A1 - Martienssen, Marion A1 - Kaiser, A. A1 - Schirmer, M. A1 - Graf, W. A1 - Kutschke, S. A1 - Schulze, R. A1 - Thiede, K. T1 - Untersuchungen zum Einsatz von Rekultivierungs-/Methanoxidationsschichten auf Deponien des Landes Sachsen-Anhalt T2 - Müll und Abfall Y1 - 2008 VL - 40 IS - 5 SP - 247 EP - 253 ER - TY - CHAP A1 - Martienssen, Marion A1 - Kukla, S. A1 - Balcke, G. U. A1 - Rohwerder, Thore A1 - Haase, K. A1 - Schirmer, M. T1 - Enhanced Natural Attenuation of MTBE: Comparison of different technologies in field experiments at the Leuna site (Germany) T2 - Second European Conference on MTBE, Barcelona, Spain, November 4 - 5, 2004 Y1 - 2004 SP - 60 EP - 64 ER - TY - CHAP A1 - Rohwerder, Thore A1 - Cenini, V. A1 - Held, C. A1 - Martienssen, Marion A1 - Lechner, U. A1 - Müller, R. H. T1 - Novel MTBE-degrading bacterial isolate from Leuna groundwater (Germany): characterization of the degradation pathway with focus on HIBA oxidation T2 - Second European Conference on MTBE, Barcelona, Spain, November 4 - 5, 2004 Y1 - 2004 SP - 47 EP - 49 ER - TY - CHAP A1 - Martienssen, Marion A1 - Schirmer, M. T1 - Comparison of different technologies to enhance the natural Attenuation of MTBE T2 - 9th International In Situ and On-Site Bioremediation Symposium, May 7 - 10, 2007, Baltimore, Maryland, USA Y1 - 2007 SN - 978-1-57477-161-9 PB - Curran CY - Redhook, NY ER - TY - GEN A1 - Schmidt, Christian A1 - Kalbus, Edda A1 - Krieg, Ronald A1 - Bayer-Raich, Marti A1 - Leschik, Sebastian A1 - Reinstorf, Frido A1 - Martienssen, Marion A1 - Schirmer, Mario T1 - Contaminant mass flow rates between groundwater, strembed sediments and surface water at the regionally contaminated site Bitterfeld T2 - Grundwasser Y1 - 2008 SN - 1430-483X VL - 13 IS - 3 SP - 133 EP - 146 ER - TY - GEN A1 - Schmidt, C. A1 - Kalbus, Edda A1 - Martienssen, Marion A1 - Schirmer, Mario T1 - The influence of heterogeneous groundwater discharge on the timescales of contaminant mass flux from streambed sediments field evidence and long-term predictions T2 - Hydrology and Earth System Sciences Discussions Y1 - 2008 SN - 1812-2116 VL - 5 IS - 2 SP - 971 EP - 1001 ER - TY - GEN A1 - Muhondwa, Jacob Paul A1 - Martienssen, Marion A1 - Burkhardt, Marko T1 - Feasibility of Anaerobic Digestion of Potato peels for Biogas as Mitigation of Greenhouse gases Emission Potential T2 - International journal of environmental research Y1 - 2015 SN - 1735-6865 VL - 9 IS - 2 SP - 481 EP - 488 ER - TY - GEN A1 - Muhondwa, Jacob Paul A1 - Martienssen, Marion A1 - Burkhardt, Marko T1 - Effect of high biowaste moisturie content on phatogens hygienization potential of in-vessel composting technology during compost production T2 - International Journal of Current Research Y1 - 2015 SN - 0975-833X VL - 7 IS - 4 SP - 15042 EP - 15048 ER - TY - GEN A1 - Böllmann, Jörg A1 - Rathsack, Kristina A1 - Martienssen, Marion T1 - The precision of bacterial quantification techniques on different kinds of environmental samples and the effect of ultrasonic treatment T2 - Journal of Microbiological Methods N2 - The precision of cell number quantification in environmental samples depends on the complexity of the sample and on the applied technique. We compared fluorescence microscopy after filtration, quantification of gene copies and the cultivation based most probable number technique for their precision. We further analyzed the effect of increasing complexity of the sample material on the precision of the different methods by using pure cultures of Pseudomonas aeruginosa, fresh water samples and sediment slurries with and without ultrasonic treatment for analyses. Microscopy reached the highest precision, which was similar between pure cultures and water samples, but lower for sediment samples due to a higher percentage of cells in clusters and flocks. The PCR based quantification was most precise for pure cultures. Water and sediment samples were similar but less precise, which might be caused by the applied DNA extraction techniques. MPN measurements were equally precise for pure cultures and water samples. For sediment slurries the precision was slightly lower. The applied ultrasonic treatment of the slurries dispersed the cell clusters and flocks, increased the precision of microscopical and MPN measurements and also increased the number of potential colony forming units. However, the culturable cell number decreased by half. For MPN quantification of viable cells in samples with a high proportion of clustered cells we therefore recommend an optimization of ultrasonic treatment and a confirmation by microscopy and cultivation to reach highest possible dispersion of the cells with a minimum of inactivation. As a result of these observations we suggest a correction factor for MPN measurements to consider the effect of sonication on complex samples. The results are most likely applicable to other complex samples such as soil or biofilms. Y1 - 2016 U6 - https://doi.org/10.1016/j.mimet.2016.05.006 SN - 0167-7012 VL - 126 SP - 42 EP - 47 ER - TY - GEN A1 - Bryant, Isaac Mbir A1 - Martienssen, Marion A1 - Burkhardt, Marko T1 - Millennium development goal on sustainable sanitation in Ghana: A reality or a mirage? T2 - African Journal of science and research Y1 - 2016 SN - 2306-5877 VL - 5 IS - 3 SP - 01 EP - 06 ER - TY - GEN A1 - Engelbrecht, Steffen A1 - Fondengcap, Mbengamina Terence A1 - Martienssen, Marion A1 - Rathsack, Kristina T1 - Highly efficient long-term storage of carrier-bound anammox biomass T2 - Water Science & Technology N2 - The anammox process is a potential alternative to the conventional nitrogen removal from wastewater. However, due to large generation times of anammox bacteria, the start-up of treatment reactors may be impeded. An efficient storage technique can handle this drawback and may be also suitable for seasonally operated treatment plants like in touristic areas. In the current study, several storage techniques were investigated with respect to its suitability for the preservation of the specific anammox activity after long-term storage. Storing conditions differed in terms of temperature, redox buffer and nutrient supplementation. The specific activity of immobilized anammox bacteria (Candidatus Kuenenia stuttgartiensis) was determined three times during a long-term preservation of 78 days and 106 days, respectively. The highest activity was ensured at a storing temperature of 4 WC, providing nitrate as redox buffer and a nutrient supplement every 23 days. Thus, 91.4% of the initial anammox activity could be preserved after a storage of 106 days. Superiority of the presented treatment condition was confirmed by a calculated nitrate-ammonium consumption rate close to the optimal ratio of 1.32. This technique provided an economical and simple method suitable for long-term storage of immobilized anammox biomass. KW - anammox KW - biofilm KW - Candidatus Kuenenia stuttgartiensis KW - long-term storage KW - reactivation Y1 - 2016 U6 - https://doi.org/10.2166/wst.2016.364 SN - 0273-1223 SN - 1996-9732 VL - 74 IS - 8 SP - 1911 EP - 1918 ER - TY - GEN A1 - Kuhn, Ramona A1 - Tóth, Elisabeth A1 - Geppert, Helmut A1 - Fischer, Thomas A1 - Martienssen, Marion T1 - Identification of the Complete Photodegradation Pathway of Ethylenediaminetetra(methylenephosphonic acid) in Aqueous Solution T2 - Clean - soil, air, water : a journal of sustainability and environmental safety N2 - This study aims at investigating the abiotic degradation pathway of ethylenediaminetetra(methylenephosphonic acid) (EDTMP) simulated applying UV irradiation. The degradation of EDTMP and formation of degradation products was determined using LC-MS and ³¹P-NMR. In the laboratory scale experiments, EDTMP was degraded within 30 min and the degradation products, iminodi(methylenephosphonic acid) (IDMP), ethylaminobis(methylenephosphonic acid) (EABMP), and amino- (methylenephosphonic acid) (AMPA), were simultaneously released. IDMP was the main degradation product of EDTMP. Therefore, we conclude that the initial cleavage of EDTMP is a heterolytically driven process, which starts the degradation process at the intramolecular C-N bond. In contrast, the main product of a possible homolytic C-C cleavage of methylaminobis(methylenephosphonic acid) could not be confirmed with either LC-MS or ³¹P-NMR. Additionally, there was no evidence for a primary attack on the C-P bond. All identified degradation products of EDTMP have been mineralized to carbon dioxide (CO₂). Three additional degradation products (M1, M2, and M3) have been found using the ³¹P-NMR analysis but have not yet been quantified using LC-MS. We assume that the unidentified degradation product M1 is related to m/z 312, M2 to m/z 341, and M3 to m/z 409. Thus we concluded that EDTMP undergoes photochemical conversion to IDMP, the main degradation product. EABMP and AMPA also accumulate, but in smaller amounts. All intermediates are further mineralized to CO₂. KW - EDTMP KW - LC-MS KW - 31P-NMR KW - Phosphonates KW - Photodegradation Y1 - 2017 U6 - https://doi.org/10.1002/clen.201500774 SN - 1863-0669 VL - 45 IS - 5 SP - 1 EP - 8 ER - TY - GEN A1 - Kuhn, Ramona A1 - Böllmann, Jörg A1 - Krahl, Kathrin A1 - Bryant, Isaac Mbir A1 - Martienssen, Marion T1 - Comparison of ten different DNA extraction procedures with respect to their suitability for environmental samples T2 - Journal of Microbiological Methods N2 - DNA extraction for molecular biological applications usually requires target optimized extraction procedures depending on the origin of the samples. For environmental samples, a range of different procedures has been developed. We compared the applicability and efficiency of ten selected DNA extraction methods published in recent literature using four different environmental samples namely: activated sludge from a domestic wastewater treatment plant, river sediment, anaerobic digestion sludge and nitrifying enrichment culture. We assessed the suitability of the extraction procedures based on both DNA yield and quality. DNA quantification was performed by both ultra violet (UV) spectrophotometry and fluorescence spectrophotometry after staining with PicoGreen. In our study, DNA yields based on UV measurement were overestimated in most cases while DNA yields from fluorescence measurements correlated well with the sample load on agarose gels of crude DNA. The quality of the DNA extracts was determined by gel electrophoresis of crude DNA and PCR products from 16S rDNA with the universal primer set 27f/1525r. It was observed that gel electrophoresis of crude DNA was not always suitable to evaluate DNA integrity and purity since interfering background substances (e.g. humic substances) were not visible. Therefore, we strongly recommend examining the DNA quality of both crude DNA and 16S rDNA PCR products by gel electrophoresis when a new extraction method is established. Summarizing, we found four out of ten extraction procedures being applicable to all tested samples without noticeable restrictions. The procedure G (according to the standard method 432_10401 of the Lower Saxony State Office for Consumer Protection and Food Safety) had the broadest application range over procedure J (published by Wilson, 2001). These were followed by procedures F (Singka et al., 2012) and A (Bourrain et al., 1999). All four extraction procedures delivered reliable and reproducible crude DNA and PCR products. From an economical point of view, all procedures tested during this study were cheaper compared to commercial DNA extraction kits. KW - DNA extraction KW - DNA quantification KW - DNA yield KW - Environmental samples KW - Gel electrophoresis KW - PicoGreen Y1 - 2017 U6 - https://doi.org/10.1016/j.mimet.2017.10.007 SN - 0167-7012 VL - 143 SP - 78 EP - 86 ER - TY - GEN A1 - Kuhn, Ramona A1 - Böllmann, Jörg A1 - Krahl, Kathrin A1 - Bryant, Isaac Mbir A1 - Martienssen, Marion T1 - Data on DNA gel sample load, gel electrophoresis, PCR and cost analysis T2 - Data in Brief N2 - The data presented in this article provide supporting information to the related research article “Comparison of ten different DNA extraction procedures with respect to their suitability for environmental samples” (revised manuscript submitted to J. Microbiol. Methods). In that article, we compared the suitability of ten selected DNA extraction methods based on DNA quality, purity, quantity and applicability to universal PCR. Here we provide the data on the specific DNA gel sample load, all unreported gel images of crude DNA and PCR results, and the complete cost analysis for all tested extraction procedures and in addition two commercial DNA extraction kits for soil and water. KW - Cost analysis KW - DNA sample load KW - Gel electrophoresis Y1 - 2018 U6 - https://doi.org/10.1016/j.dib.2017.11.082 SN - 2352-3409 VL - Vol. 16 SP - 732 EP - 751 ER - TY - GEN A1 - Domańska, Magdalena A1 - Kuhn, Ramona A1 - Łomotowski, Janusz A1 - Stańczyk, Ewa T1 - FISH method for identification of microbes in wastewater distribution systems T2 - Environmental Protection Engineering N2 - The FISH (fluorescence in situ hybridization) method is widely used to identify various types of cells. In comparison to cultivation-dependent methods, identification of microbes by FISH is easier and generally takes several hours. This paper presents a review of improvements to the FISH method, its advantages and disadvantages, as well as examples of applications. Particular consideration was given to the efficiency of microbes identification in samples taken from sewerage. The effectiveness of the method was confirmed by the results obtained in samples from the membrane bioreactor (MBR). Y1 - 2014 SN - 0324-8828 VL - 40 IS - 3 SP - 151 EP - 160 ER - TY - GEN A1 - Adonadaga, Melvin-Guy A1 - Martienssen, Marion T1 - In situ identification of filamentous beacteria from activated studge wastewater treatment plant in Ghana T2 - Journal of Applied & Environmental Microbiology Y1 - 2015 SN - 2373-6712 SN - 2373-6747 VL - 3 IS - 3 SP - 75 EP - 81 ER - TY - GEN A1 - Adonadaga, Melvin-Guy A1 - Martienssen, Marion T1 - Reliability of morphological approach for bulking filamentous bacteria identification in activated sludge T2 - International Journal of Applied Science and Technology Y1 - 2016 SN - 2221-0997 SN - 2221-1004 VL - 6 IS - 2 SP - 30 EP - 37 ER -