TY - CHAP A1 - Pohlert, M. A1 - Martienssen, Marion A1 - Weiß, H. A1 - Trabitzsch, R. A1 - Geistlinger, Helmut T1 - SF6-Gastracerversuche zur Abschätzung der Raumwirkung bei Direktgasinjektionen zur Grundwassersanierung T2 - Behandlungstechnologien für bergbaubeeinflusste Wässer, Proceedingsband zu den Workshops am Geologischen Institut der TU Bergakademie Freiberg am 12. und 13. Juni 2008 Y1 - 2008 SP - 1141 EP - 1148 PB - Institut für Geologie CY - Freiberg ER - TY - GEN A1 - Koch, Roland A1 - Straub, Andrea A1 - Mangold, Sylke A1 - Schöpke, Ralph A1 - Martienssen, Marion T1 - Tausalz, Temperatur und chemische P-Fällung T2 - Wasserwirtschaft, Wassertechnik : wwt Y1 - 2008 SN - 1438-5716 IS - 11/12 SP - 41 EP - 46 ER - TY - GEN A1 - Leschik, Sebastian A1 - Musolff, Andreas A1 - Martienssen, Marion A1 - Krieg, Ronald A1 - Bayer-Raich, Marti A1 - Reinstorf, Frido A1 - Strauch, Gerhard A1 - Schirmer, Mario T1 - Investigation of sewer exfiltration using integral pumping tests and wastewater indicators Y1 - 2009 U6 - https://doi.org/10.1016/j.jconhyd.2009.10.001 ER - TY - GEN A1 - Leschik, Sebastian A1 - Musolff, Andreas A1 - Krieg, Ronald A1 - Martienssen, Marion A1 - Bayer-Raich, Marti A1 - Strauch, Gerhard A1 - Schirmer, Mario A1 - Reinstorf, Frido T1 - Application of integral pumping tests to investigate the influence of a losing stream on groundwater quality Y1 - 2009 U6 - https://doi.org/10.5194/hess-13-1765-2009 ER - TY - GEN A1 - Schmidt, C. A1 - Kalbus, Edda A1 - Martienssen, Marion A1 - Schirmer, M. T1 - The influence of heterogeneous groundwater discharge an the timescales of contaminat mass flux from streambed sediments field evidence and long-term predictions T2 - Hydrology and Earth Systems Sciences Discussion Y1 - 2008 VL - 5 IS - 2 SP - 971 EP - 1001 ER - TY - GEN A1 - Martienssen, Marion A1 - Kukla, S. A1 - Balcke, G. U. A1 - Hasselwander, E. A1 - Schirmer, M. T1 - Evidence of natural occurring MTBE biodegradation by analysing metabolites and biodegradation products Y1 - 2006 ER - TY - GEN A1 - Beckmann, Annika A1 - Gerhardt, Matthias A1 - Zittwitz, Mark A1 - Martienssen, Marion A1 - Krieg, Ronald A1 - Geistlinger, Helmut A1 - Schirmer, Mario T1 - Das Oxywall-Projekt: Anwendung eines Verfahrens zur Direktgasinjektion von Sauerstoff zur in situ Sanierung von organisch kontaminierten Grundwässern T2 - Altlastenspektrum Y1 - 2007 SN - 0942-3818 VL - 16 IS - 8 SP - 153 EP - 159 ER - TY - PAT A1 - Ringpfeil, Manfred A1 - Gerhardt, Matthias A1 - Zittwitz, Mark A1 - Beckmann, Annika A1 - Geistlinger, Helmut A1 - Schirmer, Mario A1 - Martienssen, Marion T1 - Verfahren und Anordnung zur Direktgasinjektion für die Sanierung von kontaminierten Börden und/oder Grundwasserleitern sowie ein entsprechendes Computerprogramm und ein entsprechendes computerlesbares Speichermedium Y1 - 2007 ER - TY - CHAP A1 - Martienssen, Marion T1 - Erste Ergebnisse aus dem F+E-Thema "Methanoxidation in Rekultivierungs-/Wasserhaushaltsschichten in Sachsen-Anhalt T2 - 3. Leipziger Deponiefachtagung, 22/23.02.2007, Leipzig, Conference proceedings Y1 - 2007 PB - HTWK CY - Leipzig ER - TY - GEN A1 - Böckelmann, Uta A1 - Janke, Andrea A1 - Kuhn, Ramona A1 - Neu, Thomas R. A1 - Wecke, Jörg A1 - Lawrence, John R. A1 - Szewzyk, Ulrich T1 - Bacterial extracellular DNA forming a defined network-like structure Y1 - 2006 ER - TY - GEN A1 - Guiot, Serge R. A1 - Kuhn, Ramona A1 - Lévesque, M. J. A1 - Cimpoia, Ruxandra T1 - Ultrastructure of a bioelectrolytic methanogenic/methanotrophic granular biofilm for the complete degradation of tetrachloroethylene in contaminated groundwater Y1 - 2007 ER - TY - GEN A1 - Guiot, Serge R. A1 - Cimpoia, Ruxandra A1 - Kuhn, Ramona A1 - Alaplantive, Aude T1 - Electrolytic Methanogenic-methanotropic coupling for Tetrachloroethylene Bioremediation: A Proof of Concept T2 - Environmental science & technology Y1 - 2008 SN - 0013-936X VL - 42 IS - 8 SP - 3011 EP - 3017 ER - TY - CHAP A1 - Nixdorf, Brigitte A1 - Becker, Katja A1 - Grüneberg, Björn A1 - Böllmann, Jörg A1 - Martienssen, Marion T1 - Stickstoffumsätze in unterschiedlichen Gewässertypen Berlin/Brandenburgs, Teil 2: Erste Ergebnisse zu einträgen und Umsätzen T2 - Erweiterte Zusammenfassungen der Jahrestagung 2011 der Deutschen Gesellschaft für Limnologie (DGL) und der deutschen und österreichischen Sektion der Societas Internationalis Limnologiae (SIL), Weihenstephan, 12. - 16. Sept. 2011 Y1 - 2012 SN - 978-3-9813095-2-2 SP - 239 EP - 244 PB - Eigenverl. der DGL CY - Hardegsen ER - TY - CHAP A1 - Böllmann, Jörg A1 - Martienssen, Marion A1 - Grüneberg, Björn T1 - Vergleich der mikrobiellen Nitratreduktion in Sediment und Pelagial eines eutrophen Flusssees und eines mesotrophen geschichteten Sees T2 - Erweiterte Zusammenfassungen der Jahrestagungder Deutschen Gesellschaft für Limnologie (DGL), Potsdam - Berlin, 09. - 13. September 2013 KW - Nitratreduktion Y1 - 2014 SN - 978-3-9813095-5-3 SP - 35 EP - 39 PB - Hardegsen CY - Eigenverlag der DGL ER - TY - GEN A1 - Göring, Daniel A1 - Martienssen, Marion T1 - Elimination von Epichlorhydrin bei der Behandlung industrieller Abwässer T2 - KA - Korrespondenz Abwasser, Abfall Y1 - 2014 SN - 1866-0029 VL - 61 IS - 5 SP - 447 EP - 455 ER - TY - CHAP A1 - Böllmann, Jörg A1 - Scholler, Markus T1 - Preliminary studies on the spread of the rust fungus Puccinia glechomatis in North America T2 - Joint Meeting of the American Phytopathological Society, the Mycological Society of America, and the Society of Nematologists, Salt Lake City, Utah, USA, August 25-29, 2001 Y1 - 2001 N1 - Phytopathology 21(2001)6 SP - S. 104 ER - TY - CHAP A1 - Böllmann, Jörg A1 - Scholler, Markus T1 - The Life Cycle of Puccinia glechomatis, a microcyclic rust on Glechoma hederacea T2 - 2002 MSA Annual Meeting Corvallis, OREGON Y1 - 2002 UR - http://msafungi.org/wp-content/uploads/Inoculum/53%283%29.pdf N1 - Inoculum : newsletter of the Mycological Society of America 53(2002)3 SP - S. 21 ER - TY - GEN A1 - Rathsack, Kristina A1 - Böllmann, Jörg A1 - Martienssen, Marion T1 - Comparative Study of Different Methods for Analyzing Denitrifying Bacteria in Fresh Water Ecosystems T2 - Journal of Water Resource and Protection N2 - Bacteria capable of denitrification play a significant role in the nitrogen cycle of freshwater ecosystems. By metabolizing nitrogen compounds they e.g. counteract the eutrophication of natural waters. To get detailed insights into the in situ turnover rates of nitrogen a reliable tool of quantification for active microorganisms is essential. In the present investigation, quantification capabilities of a molecular tool (Polymerase Chain Reaction—PCR) and a cultivation based tool (Most probable number—MPN) were investigated and compared. The total bacterial concentration yielded by the molecular PCR approach was up to 6-fold higher compared to the results of the MPN approach. However, the portion of culturable denitrifying bacteria compared to the number of specific gene copies (nirS) was much lower. Depending on the environmental conditions, the difference between the PCR and the MPN approach was up to three orders of magnitude. From lab scale experiments with a pure P. aeroginosa strain it can be concludes, that these differences are not the result of inappropriate culture conditions but rather reflect the portion of so called viable but not culturable bacteria (VBNC). Low nitrate concentrations as found in many fresh water ecosystems induced a significant increase in the portion of non culturable denitrifying bacteria. Referred to the investigation of dynamic populations, the number of metabolic active bacteria is represented by the MPN rather than by the PCR approach. Y1 - 2014 SN - 1945-3094 SN - 1945-3108 VL - 6 IS - 6 SP - 609 EP - 617 ER - TY - GEN A1 - Rathsack, Kristina T1 - Unerwünschte Bewohner : Wasserasseln und andere Makroorganismen in Trinkwasserversorgungsnetzen T2 - IKZ Haustechnik : Magazin für Gebäude- und Energietechnik, Sonderheft Trinkwasserhygiene 2013 Y1 - 2013 IS - Sonderheft ER - TY - GEN A1 - Böllmann, Jörg A1 - Schöller, Markus T1 - Life cycle and life strategy features of Puccinia glechomatis (Uredinales) favorable for extending the natural range of distribution T2 - Mycoscience Y1 - 2006 U6 - https://doi.org/10.1007/s10267-006-0282-z SN - 1340-3540 VL - 47 IS - 3 SP - 152 EP - 158 ER - TY - GEN A1 - Rathsack, Kristina A1 - Rathsack, Udo T1 - Besiedlung von Wasserversorgungssystemen durch Asseln und andere Invertebraten : Weiterentwickeltes Modell und Messsystem biologischer Prozesse T2 - GWF : Wasser, Abwasser Y1 - 2010 SN - 0016-3651 VL - 151 IS - 2 SP - 200 EP - 207 ER - TY - GEN A1 - Rathsack, Kristina A1 - Rathsack, Udo T1 - Besiedlung von Wasserversorgungssystemen durch Asseln und andere Invertebraten T2 - GWF : Wasser, Abwasser Y1 - 2009 SN - 0016-3651 VL - 150 IS - 6 SP - 451 EP - 458 ER - TY - GEN A1 - Rathsack, Kristina A1 - Stackebrandt, E. A1 - Reitner, Joachim A1 - Schumann, G. T1 - Microorganisms Isolated from Deep Sea Low-temperature Influenced Oceanic Crust Basalts and Sediment Samples Collected along the Mid-Atlantic Ridge T2 - Geomicrobiology journal Y1 - 2009 SN - 0149-0451 VL - 26 IS - 4 SP - 264 EP - 274 ER - TY - GEN A1 - Nayernia, Karim A1 - Nolte, Jessica A1 - Michelmann, Hans W. A1 - Lee, Jae Ho A1 - Rathsack, Kristina A1 - Drusenheimer, Nadja A1 - Dev, Arvind A1 - Wulf, Gerald A1 - Ehrmann, Ingrid E. A1 - Elliott, David J. A1 - Okpanyi, Vera A1 - Zechner, Ulrich A1 - Haaf, Thomas A1 - Meinhardt, Andreas A1 - Engel, Wolfgang T1 - In Vitro-Differentiated Embryonic Stem Cells Give Rise to Male Gametes that Can Generate Offspring Mice T2 - Developmental Cell Y1 - 2006 SN - 1534-5807 SN - 1878-1551 VL - 11 IS - 1 SP - 125 EP - 132 ER - TY - THES A1 - Rathsack, Kristina T1 - Microbial diversity within the low-temperature influenced deep marine biosphere along the Mid-Atlantic-Ridge Y1 - 2010 ER - TY - GEN A1 - Nolte, Jessica A1 - Rathsack, Kristina A1 - Drusenheimer, Nadja A1 - Dev, Arvind A1 - Zechner, Ulrich A1 - Haaf, Thomas A1 - Meinhardt, Andreas A1 - Michelmann, Hans W. A1 - Nayernia, Karim A1 - Engel, Wolfgang T1 - Männliche Keimzellen aus embryonalen Stammzellen T2 - Medizinische Genetik Y1 - 2007 SN - 0936-5931 VL - 19 IS - 1 SP - 10 EP - 17 ER - TY - CHAP A1 - Böllmann, Jörg T1 - Die Bedeutung von Anthropochorie und die Verbreitung von infiziertem Wirtsgewebe am Beispiel des Rostpilzes Puccinia glechomatis T2 - Treffpunkt biologische Vielfalt, aktuelle Forschung im Rahmen des Übereinkommens über die biologische Vielfalt vorgestellt auf einer wissenschaftlichen Expertentagung an der Internationalen Naturschutzakademie Insel Vilm vom 21. - 25. August 2006 Y1 - 2007 UR - http://www.bfn.de/fileadmin/MDB/documents/service/skript207.pdf SP - 107 EP - 112 PB - Bundesamt für Naturschutz CY - Bonn [u.a.] ER - TY - CHAP A1 - Scholler, Markus A1 - Böllmann, Jörg T1 - Puccinia glechomatis and P. lagenophorae in North America: invasion patterns, life cycle and life strategies T2 - Mycological Society of America (MSA) annual meeting, Hilo (Hawaii), 2006 Y1 - 2006 UR - http://msafungi.org/wp-content/uploads/Inoculum/56%284%29.pdf N1 - Inoculum : newsletter of the Mycological Society of America 56(2006)4 ER - TY - CHAP A1 - Geistlinger, Helmut A1 - Beckmann, Annika A1 - Gerhardt, Matthias A1 - Martienssen, Marion A1 - Schirmer, Mario T1 - Das Feldexperiment OXYWALL: Direktgasinjektion von Sauerstoff zur in situ Sanierung von organisch kontaminierten Grundwässern T2 - Behandlungstechnologien für bergbaubeeinflusste Wässer, GIS - Geowissenschaftliche Anwendungen und Entwicklungen, Proceedingsband zu den Workshops am Geologischen Institut der TU Bergakademie Freiberg am 14. und 15. Juni 2007 Y1 - 2007 SP - 99 EP - 106 PB - Inst. für Geologie CY - Freiberg ER - TY - THES A1 - Fichtner, Nicole T1 - Untersuchung zur Leistungsfähigkeit von Biofilmverfahren mit frei beweglichen Aufwuchskörpern bei der kommunalen Abwasserbehandlung Y1 - 2015 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:co1-opus4-34765 CY - Cottbus ER - TY - CHAP A1 - Geistlinger, Helmut A1 - Lazik, D. A1 - Eisemann, D. A1 - Beckmann, Annika A1 - Martienssen, Marion A1 - Schirmer, Mario A1 - Zittwitz, Mark A1 - Gerhardt, Matthias T1 - Reaktive Gaswände mittels Direktgasinjektion: Vom Bench-Scale zum Field-Scale (OXYWALL) T2 - Behandlungstechnologien für bergbaubeeinflusste Wässer, GIS - geowissenschaftliche Anwendungen und Entwicklungen, Proceedingsband zu den Workshops am Geologischen Institut der TU Bergakademie Freiberg am 22. und 23. Juni 2006 Y1 - 2006 SP - 159 EP - 163 PB - Inst. für Geologie CY - Freiberg ER - TY - PAT A1 - Ringpfeil, Manfred A1 - Gerhardt, Matthias A1 - Zittwitz, Mark A1 - Beckmann, Annika A1 - Geistlinger, Helmut A1 - Schirmer, Mario A1 - Martienssen, Marion T1 - Verfahren und Anordnung zur Direktgasinjektion für die Sanierung von kontaminierten Böden und /oder Grundwasserleitern sowie ein entsprechendes Computerprogramm und ein entsprechendes computerlesbares Speichermedium Y1 - 2007 ER - TY - CHAP A1 - Martienssen, Marion A1 - König, W. T1 - Online-Bestimmung von Nitrifikationsinhibitoren in biologischen Kläranlagen T2 - Technische Systeme für Biotechnologie und Umwelt, 11. Heiligenstädter Kolloquium, Heilbad Heiligenstadt, 30.09. - 02.10.2002 Y1 - 2003 SN - 3-00-011287-1 SP - 205 EP - 210 PB - Institut für Bioprozess- und Analysenmesstechnik CY - Heiligenstadt ER - TY - GEN A1 - Martienssen, Marion A1 - Kaiser, A. A1 - Schirmer, M. A1 - Graf, W. A1 - Kutschke, S. A1 - Schulze, R. A1 - Thiede, K. T1 - Untersuchungen zum Einsatz von Rekultivierungs-/Methanoxidationsschichten auf Deponien des Landes Sachsen-Anhalt T2 - Müll und Abfall Y1 - 2008 VL - 40 IS - 5 SP - 247 EP - 253 ER - TY - CHAP A1 - Martienssen, Marion A1 - Kukla, S. A1 - Balcke, G. U. A1 - Rohwerder, Thore A1 - Haase, K. A1 - Schirmer, M. T1 - Enhanced Natural Attenuation of MTBE: Comparison of different technologies in field experiments at the Leuna site (Germany) T2 - Second European Conference on MTBE, Barcelona, Spain, November 4 - 5, 2004 Y1 - 2004 SP - 60 EP - 64 ER - TY - CHAP A1 - Rohwerder, Thore A1 - Cenini, V. A1 - Held, C. A1 - Martienssen, Marion A1 - Lechner, U. A1 - Müller, R. H. T1 - Novel MTBE-degrading bacterial isolate from Leuna groundwater (Germany): characterization of the degradation pathway with focus on HIBA oxidation T2 - Second European Conference on MTBE, Barcelona, Spain, November 4 - 5, 2004 Y1 - 2004 SP - 47 EP - 49 ER - TY - CHAP A1 - Martienssen, Marion A1 - Schirmer, M. T1 - Comparison of different technologies to enhance the natural Attenuation of MTBE T2 - 9th International In Situ and On-Site Bioremediation Symposium, May 7 - 10, 2007, Baltimore, Maryland, USA Y1 - 2007 SN - 978-1-57477-161-9 PB - Curran CY - Redhook, NY ER - TY - GEN A1 - Schmidt, Christian A1 - Kalbus, Edda A1 - Krieg, Ronald A1 - Bayer-Raich, Marti A1 - Leschik, Sebastian A1 - Reinstorf, Frido A1 - Martienssen, Marion A1 - Schirmer, Mario T1 - Contaminant mass flow rates between groundwater, strembed sediments and surface water at the regionally contaminated site Bitterfeld T2 - Grundwasser Y1 - 2008 SN - 1430-483X VL - 13 IS - 3 SP - 133 EP - 146 ER - TY - GEN A1 - Schmidt, C. A1 - Kalbus, Edda A1 - Martienssen, Marion A1 - Schirmer, Mario T1 - The influence of heterogeneous groundwater discharge on the timescales of contaminant mass flux from streambed sediments field evidence and long-term predictions T2 - Hydrology and Earth System Sciences Discussions Y1 - 2008 SN - 1812-2116 VL - 5 IS - 2 SP - 971 EP - 1001 ER - TY - GEN A1 - Muhondwa, Jacob Paul A1 - Martienssen, Marion A1 - Burkhardt, Marko T1 - Feasibility of Anaerobic Digestion of Potato peels for Biogas as Mitigation of Greenhouse gases Emission Potential T2 - International journal of environmental research Y1 - 2015 SN - 1735-6865 VL - 9 IS - 2 SP - 481 EP - 488 ER - TY - GEN A1 - Muhondwa, Jacob Paul A1 - Martienssen, Marion A1 - Burkhardt, Marko T1 - Effect of high biowaste moisturie content on phatogens hygienization potential of in-vessel composting technology during compost production T2 - International Journal of Current Research Y1 - 2015 SN - 0975-833X VL - 7 IS - 4 SP - 15042 EP - 15048 ER - TY - GEN A1 - Böllmann, Jörg A1 - Rathsack, Kristina A1 - Martienssen, Marion T1 - The precision of bacterial quantification techniques on different kinds of environmental samples and the effect of ultrasonic treatment T2 - Journal of Microbiological Methods N2 - The precision of cell number quantification in environmental samples depends on the complexity of the sample and on the applied technique. We compared fluorescence microscopy after filtration, quantification of gene copies and the cultivation based most probable number technique for their precision. We further analyzed the effect of increasing complexity of the sample material on the precision of the different methods by using pure cultures of Pseudomonas aeruginosa, fresh water samples and sediment slurries with and without ultrasonic treatment for analyses. Microscopy reached the highest precision, which was similar between pure cultures and water samples, but lower for sediment samples due to a higher percentage of cells in clusters and flocks. The PCR based quantification was most precise for pure cultures. Water and sediment samples were similar but less precise, which might be caused by the applied DNA extraction techniques. MPN measurements were equally precise for pure cultures and water samples. For sediment slurries the precision was slightly lower. The applied ultrasonic treatment of the slurries dispersed the cell clusters and flocks, increased the precision of microscopical and MPN measurements and also increased the number of potential colony forming units. However, the culturable cell number decreased by half. For MPN quantification of viable cells in samples with a high proportion of clustered cells we therefore recommend an optimization of ultrasonic treatment and a confirmation by microscopy and cultivation to reach highest possible dispersion of the cells with a minimum of inactivation. As a result of these observations we suggest a correction factor for MPN measurements to consider the effect of sonication on complex samples. The results are most likely applicable to other complex samples such as soil or biofilms. Y1 - 2016 U6 - https://doi.org/10.1016/j.mimet.2016.05.006 SN - 0167-7012 VL - 126 SP - 42 EP - 47 ER - TY - GEN A1 - Bryant, Isaac Mbir A1 - Martienssen, Marion A1 - Burkhardt, Marko T1 - Millennium development goal on sustainable sanitation in Ghana: A reality or a mirage? T2 - African Journal of science and research Y1 - 2016 SN - 2306-5877 VL - 5 IS - 3 SP - 01 EP - 06 ER - TY - GEN A1 - Engelbrecht, Steffen A1 - Fondengcap, Mbengamina Terence A1 - Martienssen, Marion A1 - Rathsack, Kristina T1 - Highly efficient long-term storage of carrier-bound anammox biomass T2 - Water Science & Technology N2 - The anammox process is a potential alternative to the conventional nitrogen removal from wastewater. However, due to large generation times of anammox bacteria, the start-up of treatment reactors may be impeded. An efficient storage technique can handle this drawback and may be also suitable for seasonally operated treatment plants like in touristic areas. In the current study, several storage techniques were investigated with respect to its suitability for the preservation of the specific anammox activity after long-term storage. Storing conditions differed in terms of temperature, redox buffer and nutrient supplementation. The specific activity of immobilized anammox bacteria (Candidatus Kuenenia stuttgartiensis) was determined three times during a long-term preservation of 78 days and 106 days, respectively. The highest activity was ensured at a storing temperature of 4 WC, providing nitrate as redox buffer and a nutrient supplement every 23 days. Thus, 91.4% of the initial anammox activity could be preserved after a storage of 106 days. Superiority of the presented treatment condition was confirmed by a calculated nitrate-ammonium consumption rate close to the optimal ratio of 1.32. This technique provided an economical and simple method suitable for long-term storage of immobilized anammox biomass. KW - anammox KW - biofilm KW - Candidatus Kuenenia stuttgartiensis KW - long-term storage KW - reactivation Y1 - 2016 U6 - https://doi.org/10.2166/wst.2016.364 SN - 0273-1223 SN - 1996-9732 VL - 74 IS - 8 SP - 1911 EP - 1918 ER - TY - GEN A1 - Kuhn, Ramona A1 - Tóth, Elisabeth A1 - Geppert, Helmut A1 - Fischer, Thomas A1 - Martienssen, Marion T1 - Identification of the Complete Photodegradation Pathway of Ethylenediaminetetra(methylenephosphonic acid) in Aqueous Solution T2 - Clean - soil, air, water : a journal of sustainability and environmental safety N2 - This study aims at investigating the abiotic degradation pathway of ethylenediaminetetra(methylenephosphonic acid) (EDTMP) simulated applying UV irradiation. The degradation of EDTMP and formation of degradation products was determined using LC-MS and ³¹P-NMR. In the laboratory scale experiments, EDTMP was degraded within 30 min and the degradation products, iminodi(methylenephosphonic acid) (IDMP), ethylaminobis(methylenephosphonic acid) (EABMP), and amino- (methylenephosphonic acid) (AMPA), were simultaneously released. IDMP was the main degradation product of EDTMP. Therefore, we conclude that the initial cleavage of EDTMP is a heterolytically driven process, which starts the degradation process at the intramolecular C-N bond. In contrast, the main product of a possible homolytic C-C cleavage of methylaminobis(methylenephosphonic acid) could not be confirmed with either LC-MS or ³¹P-NMR. Additionally, there was no evidence for a primary attack on the C-P bond. All identified degradation products of EDTMP have been mineralized to carbon dioxide (CO₂). Three additional degradation products (M1, M2, and M3) have been found using the ³¹P-NMR analysis but have not yet been quantified using LC-MS. We assume that the unidentified degradation product M1 is related to m/z 312, M2 to m/z 341, and M3 to m/z 409. Thus we concluded that EDTMP undergoes photochemical conversion to IDMP, the main degradation product. EABMP and AMPA also accumulate, but in smaller amounts. All intermediates are further mineralized to CO₂. KW - EDTMP KW - LC-MS KW - 31P-NMR KW - Phosphonates KW - Photodegradation Y1 - 2017 U6 - https://doi.org/10.1002/clen.201500774 SN - 1863-0669 VL - 45 IS - 5 SP - 1 EP - 8 ER - TY - GEN A1 - Kuhn, Ramona A1 - Böllmann, Jörg A1 - Krahl, Kathrin A1 - Bryant, Isaac Mbir A1 - Martienssen, Marion T1 - Comparison of ten different DNA extraction procedures with respect to their suitability for environmental samples T2 - Journal of Microbiological Methods N2 - DNA extraction for molecular biological applications usually requires target optimized extraction procedures depending on the origin of the samples. For environmental samples, a range of different procedures has been developed. We compared the applicability and efficiency of ten selected DNA extraction methods published in recent literature using four different environmental samples namely: activated sludge from a domestic wastewater treatment plant, river sediment, anaerobic digestion sludge and nitrifying enrichment culture. We assessed the suitability of the extraction procedures based on both DNA yield and quality. DNA quantification was performed by both ultra violet (UV) spectrophotometry and fluorescence spectrophotometry after staining with PicoGreen. In our study, DNA yields based on UV measurement were overestimated in most cases while DNA yields from fluorescence measurements correlated well with the sample load on agarose gels of crude DNA. The quality of the DNA extracts was determined by gel electrophoresis of crude DNA and PCR products from 16S rDNA with the universal primer set 27f/1525r. It was observed that gel electrophoresis of crude DNA was not always suitable to evaluate DNA integrity and purity since interfering background substances (e.g. humic substances) were not visible. Therefore, we strongly recommend examining the DNA quality of both crude DNA and 16S rDNA PCR products by gel electrophoresis when a new extraction method is established. Summarizing, we found four out of ten extraction procedures being applicable to all tested samples without noticeable restrictions. The procedure G (according to the standard method 432_10401 of the Lower Saxony State Office for Consumer Protection and Food Safety) had the broadest application range over procedure J (published by Wilson, 2001). These were followed by procedures F (Singka et al., 2012) and A (Bourrain et al., 1999). All four extraction procedures delivered reliable and reproducible crude DNA and PCR products. From an economical point of view, all procedures tested during this study were cheaper compared to commercial DNA extraction kits. KW - DNA extraction KW - DNA quantification KW - DNA yield KW - Environmental samples KW - Gel electrophoresis KW - PicoGreen Y1 - 2017 U6 - https://doi.org/10.1016/j.mimet.2017.10.007 SN - 0167-7012 VL - 143 SP - 78 EP - 86 ER - TY - GEN A1 - Kuhn, Ramona A1 - Böllmann, Jörg A1 - Krahl, Kathrin A1 - Bryant, Isaac Mbir A1 - Martienssen, Marion T1 - Data on DNA gel sample load, gel electrophoresis, PCR and cost analysis T2 - Data in Brief N2 - The data presented in this article provide supporting information to the related research article “Comparison of ten different DNA extraction procedures with respect to their suitability for environmental samples” (revised manuscript submitted to J. Microbiol. Methods). In that article, we compared the suitability of ten selected DNA extraction methods based on DNA quality, purity, quantity and applicability to universal PCR. Here we provide the data on the specific DNA gel sample load, all unreported gel images of crude DNA and PCR results, and the complete cost analysis for all tested extraction procedures and in addition two commercial DNA extraction kits for soil and water. KW - Cost analysis KW - DNA sample load KW - Gel electrophoresis Y1 - 2018 U6 - https://doi.org/10.1016/j.dib.2017.11.082 SN - 2352-3409 VL - Vol. 16 SP - 732 EP - 751 ER - TY - GEN A1 - Domańska, Magdalena A1 - Kuhn, Ramona A1 - Łomotowski, Janusz A1 - Stańczyk, Ewa T1 - FISH method for identification of microbes in wastewater distribution systems T2 - Environmental Protection Engineering N2 - The FISH (fluorescence in situ hybridization) method is widely used to identify various types of cells. In comparison to cultivation-dependent methods, identification of microbes by FISH is easier and generally takes several hours. This paper presents a review of improvements to the FISH method, its advantages and disadvantages, as well as examples of applications. Particular consideration was given to the efficiency of microbes identification in samples taken from sewerage. The effectiveness of the method was confirmed by the results obtained in samples from the membrane bioreactor (MBR). Y1 - 2014 SN - 0324-8828 VL - 40 IS - 3 SP - 151 EP - 160 ER - TY - GEN A1 - Adonadaga, Melvin-Guy A1 - Martienssen, Marion T1 - In situ identification of filamentous beacteria from activated studge wastewater treatment plant in Ghana T2 - Journal of Applied & Environmental Microbiology Y1 - 2015 SN - 2373-6712 SN - 2373-6747 VL - 3 IS - 3 SP - 75 EP - 81 ER - TY - GEN A1 - Adonadaga, Melvin-Guy A1 - Martienssen, Marion T1 - Reliability of morphological approach for bulking filamentous bacteria identification in activated sludge T2 - International Journal of Applied Science and Technology Y1 - 2016 SN - 2221-0997 SN - 2221-1004 VL - 6 IS - 2 SP - 30 EP - 37 ER - TY - GEN A1 - Adonadaga, Melvin-Guy A1 - Ampadu, Boateng A1 - Martienssen, Marion T1 - Effect of sludge loading and dissolved oxygen concentration on proliferation of Thiotrix and Eikelboom Types 1851 and 0041 in activated sludge wasterwater treatment plants T2 - International Journal of Applied Science and Technology Y1 - 2016 SN - 2373-6712 SN - 2373-6747 VL - 6 IS - 4 SP - 08 EP - 13 ER - TY - GEN A1 - Kuhn, Ramona A1 - Jensch, Robert A1 - Bryant, Isaac Mbir A1 - Fischer, Thomas A1 - Liebsch, Stephan A1 - Martienssen, Marion T1 - The influence of selected bivalent metal ions on the photolysis of diethylenetriamine penta(methylenephosphonic acid) T2 - Chemosphere N2 - DTPMP is predominantly utilized as scale inhibitor. We investigated the reaction rates and degradation mechanism of DTPMP with and without addition of Fe²⁺, Mg²⁺ and Ca²⁺ by performing LC/MS and ³¹P-NMR analyses. DTPMP undergoes conversion with and without addition of bivalent metal ions. The initial cleavage of DTPMP is initiated at the C-N bond leading to release of IDMP as its major breakdown product. The release of smaller quantities of EABMP and AMPA confirmed the nucleophilic attack on the DTPMP amines. Oxidation of Fe²⁺ to Fe³⁺ during the initial 30 min indicated an intramolecular electron transfer changing the electron density distribution at the nitrogen centre, which increased the radical attack during UV irradiation. Independent of the fact that Fe acted as catalyst and Mg²⁺ and Ca²⁺ acted as reactants, we found no significant differences in their degradation mechanisms. However, the reaction rates were strongly affected by the addition of the bivalent metal ions as Fe²⁺ accelerated most DTPMP degradation followed by Mg²⁺ and Ca²⁺. The UV treatment without metal ion addition was four times slower compared with Fe²⁺ addition. We conclude that in environments rich in ferrous iron and/or at reduced redox potential, photolysis of DTPMP will be catalysed by iron and will lead to accumulation of IDMP, EABMP and AMPA and several other none-quantifiable breakdown products. KW - DTPMP KW - Ferrous ion KW - LC/MS KW - Phosphonates KW - Photolysis Y1 - 2018 U6 - https://doi.org/10.1016/j.chemosphere.2018.07.033 SN - 0045-6535 IS - 210 SP - 726 EP - 733 ER - TY - THES A1 - Coban, Oksana T1 - Microbial nitrogen transformations in constructed wetlands treating contaminated groundwater Y1 - 2015 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:co1-opus4-35006 CY - Cottbus ; Senftenberg ER - TY - THES A1 - Mahyar, Ali T1 - Development and application of a planar falling film reactor for detoxification of Cyanide containing wastewaters by means of ozonation and photocatalytic ozonation Y1 - 2016 CY - Cottbus ; Senftenberg ER - TY - THES A1 - Abdallat, Ghaida T1 - Optimization of nitrogen removal in various vertical flow constructed wetland designs and application of treated wastewater for reuse in irrigation in Jordan Y1 - 2016 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:co1-opus4-37987 CY - Cottbus ; Senftenberg ER - TY - GEN A1 - Engelbrecht, Steffen A1 - Mozooni, Mohammad A1 - Rathsack, Kristina A1 - Böllmann, Jörg A1 - Martienssen, Marion T1 - Effect of increasing salinity to adapted and non-adapted Anammox biofilms T2 - Environmental Technology N2 - The Anammox process is an efficient low energy alternative for the elimination of nitrogen from wastewater. The process is already in use for side stream applications. However, some industrial wastewaters, e.g. from textile industry are highly saline. This may be a limit for the application of the Anammox process. The aim of this study was to evaluate the effects of different NaCl concentrations on the efficiency of adapted and non-adapted Anammox biofilms. The tested NaCl concentrations ranged from 0 to 50 g NaCl*L⁻¹. Concentrations below 30 g NaCl*L⁻¹did not significantly result in different nitrogen removal rates between adapted and non-adapted bacteria. However, adapted bacteria were significantly more resilient to salt at higher concentrations (40 and 50g NaCl*L⁻¹). The IC50 for adapted and non-adapted Anammox bacteria were 19.99 and 20.30 g NaCl*L⁻¹, respectively. Whereas adapted biomass depletes the nitrogen in ratios of NO-₂/NH+₄ around 1.20 indicating a mainly Anammox-driven consumption of the nitrogen, the ratio increases to 2.21 at 40 g NaCl*L⁻¹ for non-adapted biomass. This indicates an increase of other processes like denitrification. At lower NaCL concentrations up to 10 g NaCl*L⁻¹, a stimulating effect of NaCl to the Anammox process has been observed. KW - Adapted KW - Anammox KW - Biofilm KW - Non-adapted KW - Salinity Y1 - 2019 U6 - https://doi.org/10.1080/09593330.2018.1455748 SN - 0959-3330 SN - 1479-487X VL - 40 IS - 22 SP - 2880 EP - 2888 ER - TY - THES A1 - Muhondwa, Jacob Paul T1 - Hygienization of enteropathogens contaminated biowaste for safer biological treatments T1 - Hygenisierung von mit enterophatogenen Mikroorganismen kontaminierten organischen Abfällen für ungefährliche biologische Verwertungsstrategien Y1 - 2016 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:co1-opus4-39529 CY - Cottbus ; Senftenberg ER - TY - THES A1 - Apau, Kwaku Boakye T1 - Developing a conceptual solution for domestic wastewater management in developing countries: Kumasi (Ghana) as a case study Y1 - 2017 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:co1-opus4-41544 CY - Cottbus ; Senftenberg ER - TY - THES A1 - Andolfo, Franco T1 - Energieeffizientes Nährstoffsplitting von flüssigen Wirtschaftsdüngern mittels Membranfiltration Y1 - 2017 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:co1-opus4-42805 CY - Cottbus ; Senftenberg ER - TY - THES A1 - Gómez Jáuregui Abdó, Juan Pablo T1 - Sustainable development of domestic water supply in emerging megacities : the case of the city of Guadalajara, Mexico Y1 - 2015 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:co1-opus4-36075 CY - Cottbus ; Senftenberg ER - TY - GEN A1 - Böllmann, Jörg A1 - Engelbrecht, Steffen A1 - Martienssen, Marion T1 - Autofluorescent characteristics of Candidatus Brocadia fulgida and the consequences for FISH and microscopic detection T2 - Systematic and Applied Microbiology N2 - An enrichment culture of Candidatus Brocadia fulgida was identified by three independent methods: analysis of autofluorescence using different microscope filter blocks and a fluorescence spectrometer, fluorescence in situ hybridization (FISH) with anammox-specific probes and partial sequencing of the 16S rDNA, hydrazine synthase hzsA and hydrazine oxidoreductase hzo. The filter block BV-2A (400–440, 470 LP, Nikon) was suitable for preliminary detection of Ca. B. fulgida. An excitation-emission matrix revealed three pairs of excitation-emission maxima: 288–330 nm, 288–478 nm and 417–478 nm. Several autofluorescent cell clusters could not be stained with DAPI or by FISH, suggesting empty but intact cells (ghost cells) or inhibited permeability. Successful staining of autofluorescent cells with the FISH probes Ban162 and Bfu613, even at higher formamide concentrations, suggested insufficient specificity of Ban162. Under certain conditions, Ca. B. fulgida lost its autofluorescence, which reduced the reliability of autofluorescence for identification and detection. Non-fluorescent Ca. Brocadia cells could not be stained with Ban162, but with Bfu613 at higher formamide concentrations, suggesting a dependency between both parameters. The phylogenetic analysis showed only good taxonomical clustering of the 16S rDNA and hzsA. In conclusion, careful consideration of autofluorescent characteristics is recommended when analysing and presenting FISH observations of Ca. B. fulgida to avoid misinterpretations and misidentifications. KW - 16S rDNA KW - Hydrazine synthase hzsA KW - Hydrazine oxidase hzo KW - FISH KW - Candidatus Kuenenia stuttgartiensi KW - Candidatus Brocadia fulgida Y1 - 2018 U6 - https://doi.org/10.1016/j.syapm.2018.09.002 SN - 1618-0984 SN - 0723-2020 VL - 42 IS - 2 SP - 135 EP - 144 ER - TY - GEN A1 - Kuhn, Ramona A1 - Krahl, Kathrin A1 - Bryant, Isaac Mbir A1 - Martienssen, Marion A1 - Böllmann, Jörg T1 - DNA-Extraktion aus mikrobiellen Umweltproben T2 - BioSpektrum Y1 - 2018 U6 - https://doi.org/10.1007/s12268-018-0963-0 SN - 1868-6249 SN - 0947-0867 VL - 24 IS - 6 SP - 596 EP - 598 ER - TY - THES A1 - Bryant, Isaac Mbir T1 - Development of single-stage solar supported hyper-thermophilic anaerobic reactor for biogas production and disinfection of black water. A pilot case study of Terterkessim slum, Elmina-Ghana. N2 - Rapid urbanisation in Ghana has resulted in individuals expanding the cities for abodes without considerations of the negative externalities these may have on the environment. One of the major challenges with rapid urbanisation is the formation of urban slums associated with lack of basic sanitation facilities. This has led to recurrent outbreak of cholera and typhoid fever. The use of a single-stage solar-supported hyper-thermophilic anaerobic biogas digester for the treatment of black water has not been investigated, hence this study. The performance of three seeding sludge under three different hyper-thermophilic temperatures (60°C, 65°C and 70°C) were tested in batch tests. The three seeding sludge were sewage sludge, sludge from maize silage and cow manure. The results from the batch tests showed cow manure at 65°C as the preferred seeding sludge and optimal hyper-thermophilic temperature. A 50 L single-stage laboratory-scale hyper-thermophilic continuous stirred tank reactor (HT-CSTR) was operated to treat only black water for 10 weeks using cow manure at 65°C as the seeding sludge and optimal hyper-thermophilic temperature. Afterwards, co-digestion of blended kitchen food waste and black water was also practised for 12 weeks. With a mean hydraulic retention time (HRT) of 23.3 days, a mean total COD removal of 86.3 % was achieved. The reactor had an average COD volumetric loading rate of 6.22 kgCOD/(m3.d) and remained uninhibited. It also had organic loading rate of 0.3 kgVS/(m3.d) and a degradation performance (R) of 5.43 kgCOD/(m3.d). Treatment of only black water produced biogas with less methane content of 34.9 % even though a stable pH of 6.9 was recorded both in the reactor and in the effluent. Co-digestion with kitchen food waste increased the percentage content of methane in the biogas by 77 % from 34.9 % to 61.8 %. The effectiveness of the HT-CSTR to hygienise the effluent for agricultural purpose was assessed by spiking the reactor with 200 ml each of 2 x 109 CFU/ml Salmonella senftenbergensis and 8 x 108 CFU/ml Escherichia coli. The HT-CSTR was able to hygienise all bacteria of Salmonella senftenbergensis and E. coli. A simulation test confirmed that between 30 minutes and 1 hour, all the cells of Salmonella senftenbergensis and E. coli in the treatment system were killed at 65 °C. Eubacteria, Methanosarcina spp., Methanomicrobium spp. and Methanococcus spp. were identified in the seeding sludge at the hyper-thermophilic temperature of 65°C. The design, construction and performance of a pilot-scale reactor in Terterkessim slum in Elmina, Ghana was based on results from the laboratory-scale HT-CSTR. It achieved 97 % removal of influent total COD and could produce about 2.52 Nm³CH₄/(kgCOD.d) which could be burned for at least 8 hours. The effluent cannot be used for cultivation of leafy vegetables such as cabbage since it had some concentrations of pathogens like Salmonella spp. and E. coli but can be used for cotton crop. T2 - Entwicklung eines einstufigen solar-gestützten hyperthermophilen Anaerobreaktors für die Biogasproduktion und Desinfektion von Schwarzwasser : Pilotstudie im Terterkessim Slum, Elmina – Ghana Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:co1-opus4-47814 ER - TY - CHAP A1 - Kuhn, Ramona A1 - Bryant, Isaac Mbir A1 - Martienssen, Marion T1 - Unreactive Phosphorus - Organophosphonates (mini review) T2 - Organic compounds N2 - Phosphonates comprise a very large group of organophosphonates including aminophosphonates. Their main chemical feature leads to complexation of earth alkaline metals and transition metals (under stoichiometric). Therefore they are very often applied as complexing agents of detergents, as compounds of industrial cleaning products, or as antiscalants in cooling water systems and desalination processes. The high consumption of phosphonates within the past two decades leads to an increased discharge into the aquatic environment, of which the environmental risk is still uncertain. To date, there are several analytical methods published to determine phosphonates from environmental samples. However, no standard method has been defined until now. Different treatment technologies to break down phosphonates have been studied extensively such as photochemical degradation, chemical degradation and other advanced oxidation processes. The results obtained from those studies gained our chemical understanding with respect to possible implementation of such pre-treatment approaches in wastewater treatment, cooling water treatment and desalination process. The biological degradation of phosphonates has been studied for more than forty years. It was demonstrated that microorganisms are capable to breakdown the C-P bond with at least three different degradation pathways. KW - Phosphonates KW - Photodegradation KW - Biodegradation KW - Analytical detection Y1 - 2019 SN - 978-93-87500-41-9 PB - Open Access eBook CY - Las Vegas, USA ET - 1. Auflage ER - TY - GEN A1 - Martienssen, Marion A1 - Böllmann, Jörg A1 - Nixdorf, Brigitte A1 - Rathsack, Kristina T1 - Calculation of hypolimnic denitrification in a dimictic freshwater lake during summer stratification T2 - Aquatic Microbial Ecology KW - Denitrification KW - freshwater Y1 - 2019 U6 - https://doi.org/10.3354/ame01911 SN - 0948-3055 SN - 1616-1564 VL - 83 IS - 2 SP - 189 EP - 201 ER - TY - GEN A1 - Kuhn, Ramona A1 - Jensch, Robert A1 - Bryant, Isaac Mbir A1 - Fischer, Thomas A1 - Liebsch, Stephan A1 - Martienssen, Marion T1 - Rapid sample clean-up procedure for aminophosphonate determination by LC/MS analysis T2 - Talanta N2 - Aminophosphonates are commonly utilised for complexing bivalent ions such as calcium and magnesium. With regard to environmental samples, the analysis of these highly polar compounds is still challenging due to matrix effects and lacking analytical standard methods. We have recently developed a LC/MS method for common aminophosphonates without derivatisation. This LC/MS method delivers precise and accurate measurement for standard samples with very low concentration of cations disturbing the analysis. However, due to matrix effects this LC/MS method requires a sample clean-up being also applicable to natural water or wastewater samples. We developed and optimised a sample clean-up procedure applying strong cation exchange resin Dowex 50WX8. This clean-up allows a single LC/MS analysis of hydroxyethelidene(diphosphonic acid) (HEDP), aminotris(methylenephosphonic acid) (ATMP), ethylenediaminetetra(methyloenephosphonic acid) (EDTMP), diethylenetriaminepenta(methylenephosphonic acid) (DTPMP) and its major intermediates amino(methylphosphonic acid) (AMPA) and iminodi(methylenephosphonic acid) (IDMP). We compared different test conditions with six aminophosphonates in either ultra pure water or tap water. The latter was used to simulate cation concentrations typical for natural waters. To elute all aminophosphonate including AMPA, ammonium acetate addition was necessary. The addition of ammonium acetate was combinable with all tested aminophosphonates and provides high sample quality for LC/MS analysis. For acceptable recovery, the smallest aminophosphonate AMPA required the highest addition of ammonium acetate (1000 mg L-1) during the sample clean-up. Finally, the optimised clean-up procedure was successfully applied to identify and quantify phosphonates from an industrial wastewater sample. The sample clean-up procedure is simple, cheap, rapid and precise and can be further combined with solid phase extraction and more sensitive LC/MS methods. KW - Phosphontes Y1 - 2019 U6 - https://doi.org/10.1016/j.talanta.2019.120454 SN - 0039-9140 VL - 208 ER - TY - GEN A1 - Kuhn, Ramona A1 - Jensch, Robert A1 - Bryant, Isaac Mbir A1 - Fischer, Thomas A1 - Liebsch, Stephan A1 - Martienssen, Marion T1 - Photodegradation of ethylenediaminetetra(methylenephosphonic acid) – The effect of the system configuration T2 - Journal of Photochemistry & Photobiology A: Chemistry N2 - Photodegradation of aminophosphonates such as ethylenediaminetetra(methylenephosphonic acid) (EDTMP) is recently assumed being the major degradation pathway in aquatic environments. Several photolysis studies were reported about EDTMP and possible breakdown products occurring in natural ecosystems. Reliable prediction of environmental photolysis of parent compounds and possible release of breakdown products requires different set-up conditions and varying the parameters influencing the photodegradation. We studied the influence of three different system configurations during UV degradation of EDTMP. These three configurations differed either in geometry and/or treated sample volumes. System 1 was equipped with a direct cooling jacket at the UV lamp. System 2 had the geometry of system 1 but there was no usage of a direct cooling jacket. System 3 was a gas-tight system with a larger sample volume. Using the chemical actinometer potassium ferrioxalate, we determined the highest photon flux for system 3 followed by system 2 and 1. In addition, we performed scavenger experiments with methanol and ascorbic acid in order to prove the dominating radical species. In system 1, the addition of methanol showed almost no effect while the ascorbic acid resulted in a reduction of 57.1% orthophosphate released. Therefore we conclude that in system 1 the radical-drive degradation of EDTMP is mainly based on superoxide radicals. In system 2 and 3 both radical species, i.e., hydroxyl radicals and superoxide radicals, contribute to the photodegradation of EDTMP. We determined different half-lives for EDTMP for the three different systems configurations. For system 1, the estimated half-life achieved was 14.09 ± 0.15 min. For system 2 and 3, the half-lives were almost similar and averaged 4.75 ± 0.05 min and 5.02 ± 0.20 min, respectively. Contrary to our assumption to also find the highest degradation rate for system 3, we found the highest degradation rate for system configuration 2 as a result of the differences in the construction and geometry of the three systems. Our findings lead us to recommend the three system configuration for different research purposes. Thus, we recommend system 1 for detailed studies on the degradation pathway of the parent compound and their breakdown products. System 2 is recommended as a suitable configuration for kinetic studies of the parent compound. And finally, we recommend the system configuration 3 for complete mass balances. The gas-tight system allows determining all soluble and gaseous compounds. KW - Phosphonates KW - Photodegradation KW - Photon flux KW - Scavenger KW - LC/MS KW - Actinometry Y1 - 2020 U6 - https://doi.org/10.1016/j.jphotochem.2019.112192 SN - 1010-6030 VL - 388 ER - TY - GEN A1 - Preuß, Volker A1 - Vornholt, Carsten A1 - Kuhn, Ramona A1 - Martienssen, Marion ED - Wessling, Matthias ED - Pinnekamp, Johannes T1 - Untersuchung zur Membrangängigkeit von Antiscalants T2 - 13. Aachener Tagung Wassertechnologie : Verfahren der Wasseraufbereitung und Abwasserbehandlung N2 - Aminophosphonate sind synthetisch hergestellte Komplexbildner, die kovalente C-P Bindungen aufweisen. Durch zusätzlich eingefügte Aminogruppen (NH2) komplexieren Amino-phosphonate besonders gut Metallionen, weshalb sie in einem sehr breiten Anwendungsspektum zum Einsatz kommen. So werden Phosphonate vielseitig als Haushalts- und Industriereiniger, aber auch als Komponenten von Kosmetika und in der Medizin verwendet. Des weiteren werden sie zur Kühlwasserkonditionierung, Stabilisierung von Peroxiden oder Bleichbädern und als Korrosionsinhibiter eingesetzt. Entsprechend der vielseitigen Anwendungsgebiete lag bereits in den früher 90igern des vergangenen Jahrhunderts die Gesamtproduktion an Phosphonaten in Europa bei über 11.000 Tonnen pro Jahr (Jaworska et al., 2002). Weltweit zeigen die Nutzung und der damit verbundene Bedarf an Phosphonaten einen immer weiter steigenden Trend. Dem entgegen steht der noch immer wenig untersuchte Verbleib und das chemische Verhalten von Phosphonaten in der Umwelt. Die gegenwärtig technisch eingesetzten Phosphonate ähneln in ihrer Struktur den bekannteren Aminocarboxylaten wie dem EDTA und NTA. Chemisch synthetisierte Phosphonate sind nur schwer biologisch abbaubar, weshalb zu einer kontinuierlichen Anreicherung in der aquatischen Umwelt kommen kann (Jaworska et al., 2002). Dies liegt unter anderem auch daran, dass Phosphonate als Phosphatersatz in großen Mengen in Waschmitteln enthalten sind und über das Abwasser in die Kläranlagen gelangen, wo sie nicht weiter biologisch abgebaut werden. Sie werden hauptsächlich durch Absorption an die Oberfläche vom Belebtschlamm aus den Abwasser entfernt. Nowack (2004) beschrieb die Toxizität von Phosphonaten in Kläranlagen als gering, verwies aber gleichzeitig darauf, dass aufgrund ihrer hohen Stabilität Phosphonate hemmend auf Proteasen und Syntheasen (Enzyme) von Mikroorganismen wirken. Der photochemische und biologische Abbau von dem Phosphonat EDTMP wurde eingehend untersucht, um dessen Umweltverhalten besser abschätzen zu können. Ziel der Untersuchungen war es den Abbauprozess zu entschlüsseln als Grundlage für die langfristige Entwicklung umweltverträglicher Aminophosphonatstrukturen. Der photochemische Abbau von EDTMP und die Bildung von Metaboliten konnte mittels LC-MS und 31P-NMR bereits aufgeklärt werden. So zeigt sich das EDTMP bereits nach wenigen Minuten im UV-Licht zerfällt und nicht mehr nachgewiesen werden kann. Als Hauptprodukt konnte das Phosphonat IDMP identifiziert werden. Als weitere Abbauprodukte konnten die Phosphonate EABMP und AMPA identifiziert werden. In allen Versuchen konnte nach 300 min UV-Behandlung mindestens 75% des Endprodukts CO2 nachgewiesen werden. Weitere drei maßgebliche Abbauprodukte wurden mit Hilfe der LC-MS gefunden. Deren chemische Struktur konnte bisher jedoch noch nicht eindeutig beschrieben werden. Im Anschluss an die UV-Experimente wurde ein Versuch mit Sonnenlicht simuliert. Insgesamt verlief der EDTMP-Abbau im Sonnenlicht wesentlich langsamer. Auch hier konnten wieder dieselben Abbauprodukte IDMP, EABMP und AMPA identifiziert werden. Zusätzlich traten auch die drei noch nicht eindeutig identifizierten Metaboliten von EDTMP auf. Aus den gewonnen Daten kann somit geschlussfolgert werden, dass EDTMP unter Einwirkung von UV-Licht abbaubar ist. Die Annahme, dass EDTMP und dessen Abbauprodukte langfristig zur Schädigung des aquatischen Ökosystems führt, kann somit nicht bestätigt werden. In weiteren Untersuchungen wurde der biologische Abbau von EDTMP und seiner photochemischen Abbauprodukten eingehend studiert. Für die Abbauversuche wurden die beiden Stämme Pseudomonas aeruginosa und Ochrobactrum sp. aus Boden isoliert. Es konnte gezeigt werden, dass sie 1mM EDTMP innerhalb von 35 Tagen zu mindestens 94% abbauen. Zusätzlich zum biologischen Abbau von EDTMP wurden auch die Verdopplungszeitung der beiden Stämme für EDTMP, IDMP, EABMP und AMAP ermittelt. Es zeigte sich, dass der Stamm P. aeruginosa für alle vier Phosphonate ein besseres wachstum zeigte als Ochrobactrum sp. Der Stamm P. aeruginosa erreichte für die Verstoffwechselung von EDTMP eine Verdopplungszeit von 10,1 Tagen, für IDMP 7,4 Tage, für EABMP 7,4 Tage und für AMPA 13,7 Tage. Der Stamm Ochrobactrum sp. erreichte für das Substrat EDTMP eine Verdopplungszeit von 11,8 Tagen, für IDMP 18,9 Tage, für EABMP 7,7 Tage und für AMPA 22,0 Tage. Offensichtlich war für beide Stämme der Abbau von AMPA der limitierende, aber nicht hemmende Schritt im biologischen Abbau. Somit kann geschlussfolgert werden, dass die Abbauprodukte der Photolyse auch biologisch abgebaut werden können. In weiteren Untersuchungen soll der biologische Abbau auf enzymatischer Ebene entschlüsselt werden. Die Identifizierung der Schüsselenzyme spielt hierbei eine wesentliche Rolle, um neuartige Phosphonatstrukturen herzuleiten, die auf der einen Seite ihre Funktionalität und Eigenschaften behalten, aber auf der anderen Seite eine erheblich verbesserte Bioverfügbarkeit aufweisen, so dass eine dauerhafte Akkumulation in der Umwelt und Folgebeeinträchtigungen minimiert ggf. ausgeschlossen werden können. KW - Antiscalant KW - Membranfiltration KW - Membrangängigkeit KW - Nanofiltration KW - Phosphonate KW - Umkehrosmose Y1 - 2019 SN - 978-3-95886-305-7 U6 - https://doi.org/10/35549368458 VL - 2019 SP - 117 EP - 123 PB - Verlagshaus Mainz GmbH Aachen CY - Aachen ET - 1. Auflage ER - TY - GEN A1 - Kuhn, Ramona A1 - Bryant, Isaac Mbir A1 - Jensch, Robert A1 - Liebsch, Stephan A1 - Martienssen, Marion T1 - Photolysis of hexamethylenediaminetetra(methylenephosphonic acid) (HDTMP) using manganese and hydrogen peroxide T2 - Emerging Contaminants N2 - Aminophosphonates such as hexamethylenediaminetreta(methylene phosphonic acid) (HDTMP) are categorised as persistent substances. They are commonly used as scale inhibitors in cooling water systems and desalination processes. After utilisation, they are often discharged into aquatic environment without pre-treatment. Advanced oxidation processes (AOP) are promising pre-treatments for industrial wastewater treatments. We investigated the photodegradation of HDTMP with or without addition of manganese (Mn2þ) and/or H2O2. Similar to results of our former photodegradation studies, we found that HDTMP also undergoes conversion with or without additives during the ultra violet (UV) irradiation. The reaction rate was most affected by the addition of H2O2, i.e. the HDTMP degradation was accelerated by a factor 3.85 compared with UV treatment without additives. The addition of Mn2þ accelerated the degradation of HDTMP only by a factor 1.53 compared with the UV treatment without additives. The combined addition of Mn2þ and H2O2 accelerated the HDTMP degradation by a factor 2.81. Interestingly, the initial cleavage is not initiated as expected at the CeN bond but at the CeP bond of the methyl carbon and the phosphorus of the methylenephosphonic acid group of HDTMP. This initial cleavage was independent whether the UV treatment was performed with or without additives. Therefore, we conclude that the degradation mechanism is similar independent of the four tested treatment conditions. We identified amino(methylenephosphonic acid) AMPA, dimethylamino(methylenephosphonic acid) DAMP and iminodi(methylenephosphonic acid) IDMP as the major breakdown products by performing LC/MS analyses. The major mineralisation products were ortho-phosphate, ammonium and carbon dioxide. The mass balances of unknown breakdown products KW - HDTMP KW - Hydrogen peroxide KW - Manganese KW - LC/MS KW - Phosphonates KW - Photolysis Y1 - 2019 U6 - https://doi.org/10.1016/j.emcon.2019.11.003 N1 - Die präsentierten Ergebnissen in diesem Artikel sind im Rahmen von zwei Abschlussarbeiten an der BTU erstellt worden. VL - 2020 IS - 6 SP - 10 EP - 19 ER - TY - GEN A1 - Bryant, Isaac Mbir A1 - Burkhardt, Marko A1 - Martienssen, Marion T1 - Comparison of performance of three diferent seeding sludge under three different hyper-thermophilic temperatures T2 - International Journal of Environmental Engineering Y1 - 2019 U6 - https://doi.org/10.1504/IJEE.2019.099979 SN - 1756-8471 SN - 1756-8463 VL - 10 IS - 1 SP - 1 EP - 16 ER - TY - GEN A1 - Furusato, Eiichi A1 - Bhuyan, Jayatu A1 - Martienssen, Marion A1 - Nixdorf, Brigitte A1 - Imamoto, Hiroomi T1 - Critical Depth Model (modified Sverdrup equation) - Design method for bubble circulation T2 - Journal of JSCE Y1 - 2019 U6 - https://doi.org/10.2208/journalofjsce.7.1_185 SN - 2187-5103 VL - 7 IS - 1 SP - 185 EP - 192 ER - TY - GEN A1 - Kuhn, Ramona A1 - Vornholt, Carsten A1 - Preuß, Volker A1 - Bryant, Isaac Mbir A1 - Martienssen, Marion T1 - Aminophosphonates in Nanofiltration and Reverse Osmosis Permeates T2 - Membranes N2 - Aminophosphonates such as aminotris(methylenephosphonic acid) (ATMP) are common constituents of antiscalants. In nanofiltration (NF) and reverse osmosis (RO) processes, ATMP prevents inorganic scaling leading to more stable membrane performance. So far, little attention has been paid to the possible permeation of aminophosphonates through NF and RO membranes. We have investigated the permeability of these membrane types for ATMP and its potential metabolites iminodi(methylenephosphonic acid) (IDMP) and amino(methylenephosphonic acid) (AMPA) with two different NF membranes (TS40 and TS80) and one RO membrane (ACM2) and three different water compositions (ultra-pure water, synthetic tap water and local tap water). We found traces of phosphonates in all investigated permeates. The highest phosphonate rejection occurred with local tap water for all three membranes investigated. Filtration experiments with a technical antiscalant formulation containing ATMP indicated similar trends of phosphonate permeability through all three membranes. We assume that the separation mechanisms of the membranes are the results of a very complex relationship between physico-chemical properties such as Donnan exclusion, feed pH, feed ionic strength and feed concentration, as well as solute–solute interactions. KW - ATMP KW - AMPA KW - phosphonates KW - nanofiltration KW - reverse osmosis KW - antiscalant KW - drinking water treatment Y1 - 2021 U6 - https://doi.org/10.3390/membranes11060446 SN - 2077-0375 VL - 11 IS - 6 SP - 1 EP - 16 ER - TY - GEN A1 - Böllmann, Jörg A1 - Martienssen, Marion T1 - Comparison of different media for the detection of denitrifying and nitrate reducing bacteria in mesotrophic aquatic environments by the most probable number method T2 - Journal of Microbiological Methods N2 - The cultivation based characterization of microbial communities and the quantification of certain functional bacterial groups is still an essential part of microbiology and microbial ecology. For plate count methods meanwhile low strength media are recommended, since they cover a broader range of different species and result in higher counts compared to established high strength media. For liquid media, as they are used for most probable number (MPN) quantifications, comparisons between high and low strength media are rare. In this study we compare the performance of different high and low strength media for the MPN quantification of nitrate reducing and denitrifying bacteria in two different fresh water environments. We also calculated the cell specific turnover rates of several denitrifying cultures previously enriched in high and low strength media from three different fresh water environments and a waste water treatment plant. For fresh water samples, our results indicate that high strength media detect higher MPN of denitrifying bacteria and in equal MPN of nitrate reducing bacteria compared to low strength media, which is in contrary to plate count techniques. For sediment samples, high and low strength media performed equal. The cell specific turnover rate was independent from the enrichment media and the media of the performance test. The cause of the lower denitrifyer MPN in low strength media remains, however, unclear. The results are important for further MPN quantifications of bacteria in nutrient poor environments and for calculations of nitrogen turnover rates by kinetical models using the number of metabolic active cells as one parameter. Y1 - 2020 U6 - https://doi.org/10.1016/j.mimet.2019.105808 SN - 1872-8359 VL - 168 ER - TY - GEN A1 - Böllmann, Jörg A1 - Martienssen, Marion T1 - Impact of pH conditions and the characteristics of two electrodialysis membranes on biofilm development under semi-realistic conditions T2 - Biofouling N2 - The reuse of treated wastewater for irrigation is of increasing importance. The Ecosave farming project developed a new photocatalytic electrodialysis process for desalination and hygienization. However, membrane scaling significantly reduces filtration efficiency. This study investigated biofilm development on anion and cation exchange membranes at a wide pH range in pre-treated wastewater. Epifluorescence microscopic quantification of the biofilm by cell counts and surface coverage together with 16S rDNA gene copy numbers showed stronger biofilm development on the anion exchange membrane (AEM) compared with the cation exchange membrane (CEM) with up to 105 cells mm−2 and 20% surface coverage after three weeks. As the AEM biofilm developed best in neutral and a slightly alkaline pH, the CEM was colonized preferably at alkaline conditions. Extreme pH conditions strongly inhibited biofilm growth, which might help to minimize the maintenance effort by creating those conditions during the operation of the dialysis cell itself. Y1 - 2021 U6 - https://doi.org/10.1080/08927014.2021.1999424 SN - 1029-2454 VL - 37 IS - 9-10 SP - 998 EP - 1005 ER - TY - GEN A1 - Kuhn, Ramona A1 - Bryant, Isaac Mbir A1 - Jensch, Robert A1 - Böllmann, Jörg T1 - Applications of Environmental Nanotechnologies in Remediation, Wastewater Treatment, Drinking Water Treatment, and Agriculture T2 - Applied Nano N2 - Today, nanotechnologies (NTs) are well established in both private households and commercial markets. NTs are fully accepted in several sectors, such as medicine and pharmacy, and in industries, such as chemistry, electricity, food production, military, and other commercial branches, due to their unique properties. With regard to the growing demands for environmental resources caused by the still-growing global population, the application of NTs is an extremely important new branch in the environmental sector, delivering several advantages. Our review provides a comprehensive overview of the current developments in environmental remediation, wastewater treatment, drinking water treatment, and agriculture. More specifically, in the section on environmental remediation, we review the application of NTs towards enhanced reductive dechlorination, removal of heavy metals and remediation of oil spills. In the section on wastewater treatment, we highlight developments in the adsorption of heavy metals and persistent substances, advanced photocatalytic degradation of common wastewater pollutants, and improvements in membrane filtration processes. In the section on drinking water treatment, we discuss applications for the enhanced disinfection of pathogens, removal of heavy metals, point-of-use treatments, and the removal of organic matter. In the final section, on agriculture, we provide an overview of precision farming and the current state of the art concerning nanofertilisers, nanopesticides, nanoherbicides, and nano(bio)sensors. Y1 - 2022 UR - https://www.mdpi.com/2673-3501/3/1/5 U6 - https://doi.org/10.3390/applnano3010005 SN - 2673-3501 VL - 3 IS - 1 SP - 54 EP - 90 ER - TY - GEN A1 - Okofo, Louis Boansi A1 - Martienssen, Marion T1 - A three-dimensional numerical groundwater flow model to assess the feasibility of managed aquifer recharge in the Tamne River basin of Ghana T2 - Hydrogeology Journal Y1 - 2022 U6 - https://doi.org/10.1007/s10040-022-02492-7 SN - 1431-2174 VL - 30 IS - 4 SP - 1071 EP - 1090 ER - TY - GEN A1 - Sohail, Norman A1 - Riedel, Ramona A1 - Dorneanu, Bogdan A1 - Arellano-Garcia, Harvey T1 - Prolonging the Life Span of Membrane in Submerged MBR by the Application of Different Anti-Biofouling Techniques T2 - Membranes N2 - The membrane bioreactor (MBR) is an efficient technology for the treatment of municipal and industrial wastewater for the last two decades. It is a single stage process with smaller footprints and a higher removal efficiency of organic compounds compared with the conventional activated sludge process. However, the major drawback of the MBR is membrane biofouling which decreases the life span of the membrane and automatically increases the operational cost. This review is exploring different anti-biofouling techniques of the state-of-the-art, i.e., quorum quenching (QQ) and model-based approaches. The former is a relatively recent strategy used to mitigate biofouling. It disrupts the cell-to-cell communication of bacteria responsible for biofouling in the sludge. For example, the two strains of bacteria Rhodococcus sp. BH4 and Pseudomonas putida are very effective in the disruption of quorum sensing (QS). Thus, they are recognized as useful QQ bacteria. Furthermore, the model-based anti-fouling strategies are also very promising in preventing biofouling at very early stages of initialization. Nevertheless, biofouling is an extremely complex phenomenon and the influence of various parameters whether physical or biological on its development is not completely understood. Advancing digital technologies, combined with novel Big Data analytics and optimization techniques offer great opportunities for creating intelligent systems that can effectively address the challenges of MBR biofouling. KW - Membrane bioreactor (MBR) KW - quorum sensing (QS) KW - quorum quenching (QQ) KW - moving bed biofilm reactor (MBBR) KW - moving bed biofilm membrane reactor (MBBMR) KW - model-based anti-fouling strategies Y1 - 2023 UR - https://www.mdpi.com/2077-0375/13/2/217 U6 - https://doi.org/10.3390/membranes13020217 SN - 2077-0375 VL - 13 IS - 2 ER - TY - GEN A1 - Riedel, Ramona A1 - Krahl, Kathrin A1 - Buder, Kai A1 - Böllmann, Jörg A1 - Braun, Burga A1 - Martienssen, Marion T1 - Novel standard biodegradation test for synthetic phosphonates T2 - Journal of Microbiological Methods N2 - Determination of biodegradation of synthetic phosphonates such as aminotris(methylenephosphonic acid) (ATMP), ethylenediamine tetra(methylenephosphonic acid) (EDTMP), or diethylenetriamine penta(methylenephosphonic acid) (DTPMP) is a great challenge. Commonly, ready biodegradability of organic substances is assessed by OECD 301 standard tests. However, due to the chemical imbalance of carbon to phosphorus synthetic phosphonates do not promote microbial growth and, thus, limiting its biodegradation. Therefore, standard OECD test methods are not always reliable to predict the real biodegradability of phosphonates. In the presented study, we report the development of a standardized batch system suitable to synthetic phosphonates such as ATMP, EDTMP, DTPMP and others. The novel standard batch test is applicable with pure strains, activated sludge from different wastewater treatment plants (i.e., municipal and industrial), and with tap water as inoculum. We optimized the required calcium and magnesium exposure levels as well as the amount of the start inoculum biomass. We demonstrated that our test also allows to determine several parameters including ortho-phosphate (o-PO43􀀀 ), total phosphorus (TP), ammonium (NH4+) and total organic carbon (TOC). In addition, also LC/MS analyses of cell-free medium is applicable for determining the mother compounds and metabolites. We applied our optimized standardized batch with selected phosphonates and evidenced that the chemical structure has a major influence of the microbial growth rates. Thus, our novel batch test overcomes drawbacks of the OECD 301 test series for determination of easy biodegradability for stoichiometric imbalanced organic compounds such as phosphonates. Y1 - 2023 U6 - https://doi.org/10.1016/j.mimet.2023.106793 SN - 1872-8359 SN - 0167-7012 VL - Vol. 212 SP - 1 EP - 13 ER - TY - THES A1 - Okofo, Louis Boansi T1 - Hydrogeological assessment for evaluating the feasibility of managed aquifer recharge in Northeastern Ghana N2 - In the Garu-Tempane area and Tamne River basin of north-eastern Ghana, granitic aquifers supply nearly 80% of annually abstracted groundwater. Rapid and diffuse recharge enters the fractured and weathered Tamnean Plutonic Suite aquifers mainly granitoid, which are the dominant rock types in the study area. However, a greater challenge to the water supply in the area is posed by global climatic changes and overexploitation due to population growth. The semi-arid nature of the area together with the factors mentioned earlier has caused water scarcity, particularly in the dry season and these have affected the livelihoods of the farmers who depend mostly on the groundwater for irrigation and domestic purposes. A promising way to balance water resources in the region is using engineering technology such as managed aquifer recharge (MAR). MAR augments water levels in water-scarce areas and represents a key tool in water supply management. For this reason, a comprehensive hydrogeological characterization involving the hydrochemistry of the groundwater, groundwater recharge process and residence time using multi-environmental tracers, and a numerical groundwater flow model was developed. Based on the hydrochemistry results, the water quality index showed that the groundwater is very suitable for drinking. However, about 10.5 % out of the 38 groundwater samples had elevated nitrate concentrations exceeding the permissible WHO drinking water limit. These are mainly agricultural areas, which might have influenced the elevated nitrate concentrations. Groundwater age dating using sulphur hexafluoride (SF6) and chlorofluorocarbons (CFCs) was used to date shallow groundwater in Ghana for the first time. The results proved that the mean residence time of groundwater was around 30 years, an indication of young groundwater and rapid groundwater renewability. The findings also showed different groundwater ages implying diffused flow systems occurring in the fractured granitic aquifer. Investigation of the groundwater recharge using stable isotopes of deuterium and oxygen-18 revealed that the main source of groundwater recharge is of meteoric origin. There were little or no contributions from the stream and ponds as they were subjected to evaporative fractionation during the dry season. The White Volta River samples and samples from two big rivers were depleted in heavy isotopes, which suggested a hydraulic connection between them and the groundwater. The numerical groundwater flow model was used to assess the feasibility of MAR and determine the maximum recharge and abstraction rates. The results showed that the aquifer had enough storage to accommodate enough volumes of floodwater without causing groundwater mounding. This shows that MAR is feasible in augmenting the water levels in the area when irrigation and domestic withdrawals are regulated. KW - Fluorchlorkohlenwasserstoffe KW - Grundwasserströmungsmodell KW - Nordost-Ghana KW - Schwefelhexafluorid KW - CFCs KW - Managed aquifer recharge KW - Northeastern Ghana KW - Numerical groundwater flow modelling KW - SF6 Y1 - 2023 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:co1-opus4-62444 CY - Cottbus ; Senftenberg ER - TY - THES A1 - Dido, Mann Samuel T1 - Use of Close-to-nature Surfactants and Microbiological Degradation for Improving the Remediation of Crude Oil Contaminated Soils in the Nigerian Niger-Delta N2 - The existence of large deposit of crude oil in the Nigerian Niger-Delta accords the region a strategic position in the country’s economy. However, activities associated with the oil and gas industry have resulted in extensive and in some instances indelible damages on the ecosystems. The destructive effect of hydrocarbon on bionetwork necessitated this study that aimed at evaluating the potential of adopting microbial and biosurfactant technology as tools for bioremediation. The research involved gas chromatography monitoring of hydrocarbon degradation in polluted soil, microbiological/molecular identification of hydrocarbon degraders and the evaluation of biosurfactant production by indigenous bacteria. The performance of four treatments involving; co-treatment with mixed bacterial consortium and close-to-nature surfactants (Bioversal QF and Bioversal UK), treatments with mixed bacteria culture, single bacteria culture and treatment with close-to-nature surfactants only were evaluated for the mineralization of hydrocarbons contained in 500g of 2% crude oil contaminated soil supplemented with Na2HPO4*2H2O, KH2PO4 and NH4Cl as nutrient sources over a period of 20 weeks. Six indigenous biosurfactant producing and/or hydrocarbon degrading bacteria strains were isolated namely: Pseudomonas aeruginosa, Bacillus sp, Achromobacter sp, Stenotrophonas sp, Lysinibacillus sp. and Delftia sp. Molecular characterization of sequenced polymerase chain reaction products of these cultured bacteria using Nucleotide Basic Local Alignment Search Tool confirmed close relationships ranging from 98.83% to 100% with those on the NCBI databank. Co-treatment with mixed bacteria culture and Bioversal UK/Bioversal QF yielded synergetic impact that enhanced 73.35% and 71.52% degradation of total petroleum hydrocarbon respectively. Treatments with close-to-nature surfactants and with mix bacterial culture recorded 53.61% and 54.58%. The use of single bacteria strains resulted in the mineralization of 46% - 62.26% total petroleum hydrocarbon while their use as a consortium yielded 54%. Liquid chromatography-Mass spectrometry assessment of extracellular polymeric substances produced by cultured bacteria strains indicated the presence of rhamnolipids (L-rhamnosyl-3-hydroxydecanoyl-3-hydroxydecanoic acid with a mass of 504.654 Da) and lipopeptides (2-(Hydroxymethyl) phenyl 6-O-palmitoyl-β-D-glucopyranoside with a mass of 524.687 Da). Extracellular polymeric substances produced yielded emulsion index ranging from 70.70% to 75% and 39% to 65% with crude oil and heptane respectively. EPS yields ranging from 1.34 g/ml to 1.5 g/ml were recorded.zeige weniger KW - Bioremediation KW - Biotenside KW - Erdöl KW - Umweltverschmutzung KW - Ökologie KW - Bioremediation KW - Biosurfactants KW - Crude oil KW - Ecology KW - Environmental pollution Y1 - 2022 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:co1-opus4-57301 CY - Cottbus ; Senftenberg ER - TY - CHAP A1 - Kuhn, Ramona A1 - Pollice, Alfieri A1 - Laera, Guiseppe A1 - Lipollis, Rosa A1 - Papa, Sergio ED - Lesjean, Boris T1 - Standard assays and metaproteomes as new approaches for functional characterization of membrane bioreactor biomass T2 - Membrane Technologies for Wastewater Treatment and Reuse, Proceedings der 2nd IWA National Young Water Professionals Conference in Berlin, 4.-6. Juni 2007 KW - Membrane bioreactors KW - biomass characterisation KW - EPS/SMP KW - metaproteomes Y1 - 2007 SN - 987-3-9811684-0-2 SP - 57 EP - 64 PB - IWA CY - Berlin ER - TY - THES A1 - Richter, Thomas T1 - Intrinsisch antimikrobielle Polyurethane : Synthesekonzepte - neue Strukturen - Charakterisierung Y1 - 2020 CY - Cottbus ; Senftenberg ER - TY - THES A1 - Mohanty, Ashirbad T1 - Effects of helophyte species and system type in treatment of artificial acid mine drainage under carbon-deficient and sulphate-reducing conditions in laboratory-scale horizontal flow constructed wetlands Y1 - 2018 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:co1-opus4-48595 ER - TY - THES A1 - Pulgarin Monsalve, Isabel Cristina T1 - Fundamentals of process design and contraol of Anaerobic Sequencing Batch Gas-Lift Reactor regarding the mixing process Y1 - 2020 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:co1-opus4-55693 ER - TY - CHAP A1 - Martienssen, Marion A1 - Schirmer, Mario T1 - Enhancing Natural Attenuation of MTBE at Contaminated Megasites T2 - NGWA Conference on MTBE and Perchlorate: Assessment, Remediation and Public Policy, 26.-27. Mai 2005, San Francisco, California Y1 - 2005 UR - http://info.ngwa.org/gwol/pdf/062281335.pdf SP - 219 EP - 229 ER - TY - GEN A1 - Coban, Oksana A1 - Kuschk, Peter A1 - Kappelmeyer, Uwe A1 - Spott, Oliver A1 - Martienssen, Marion A1 - Jetten, Mike S. M. A1 - Knöller, Kay T1 - Nitrogen transforming community in a horizontal subsurface-flow constructed wetland T2 - Water research Y1 - 2015 U6 - https://doi.org/0.1016/j.watres.2015.02.018 SN - 1879-2448 IS - 74 SP - 203 EP - 212 ER - TY - CHAP A1 - Rücker, Jacqueline A1 - Knie, Matthias A1 - Voss, Maren A1 - Martienssen, Marion A1 - Grüneberg, Björn A1 - Kolzau, Sebastian A1 - Nixdorf, Brigitte T1 - Abschätzung des Stickstoffeintrages durch planktische Cyanobakterien (Nostocales) T2 - Erweiterte Zusammenfassungen der Jahrestagung der Deutschen Limnologischen Gesellschaft (DGL) in Essen 2015 KW - Stickstofffixierung KW - Licht Y1 - 2016 SN - 978-3-9813095-8-4 SP - 170 EP - 177 PB - Eigenverlag der DGL CY - Hardegsen ER - TY - CHAP A1 - Martienssen, Marion A1 - Schirmer, Mario T1 - Enhanced Natural Attenuation of MTBE - Laboratory studies and its verification in field experiments - The METLEN Projectat the Leuna Site T2 - First European Conference on MTBE, conference proceedings, September 8 - 9, 2003 Y1 - 2003 SN - 3-934253-24-5 SP - 88 EP - 94 PB - Forum für Abfallwirtschaft und Altlasten e.V. CY - Pirna ET - 1. Auflage ER - TY - CHAP A1 - Martienssen, Marion ED - Kühle-Weidemeier, Matthias T1 - Alternative biologisch aktivierte Oberflächenabdichtungen für kleinere Deponien T2 - Tagungsband, Praxistagung Deponie 2005, 7. und 8. Dezember 2004 Y1 - 2005 SN - 3-86537-284-8 SP - 136 EP - 144 PB - Cuvillier Verlag CY - Göttingen ET - 1. Auflage ER - TY - GEN A1 - Kuhn, Ramona A1 - Bryant, Isaac Mbir A1 - Martienssen, Marion T1 - Supplementary data on rapid sample clean-up procedure for aminophosphonate determination by LC/MS analysis T2 - MethodsX N2 - Minimising matrix effects through high sample purity is of major importance for LC/MS analysis. Here we provide supplementary data and protocols related to the article “Rapid sample clean-up procedure of aminophosphonates for LC/MS analysis”(revised article submitted to Talanta) [1] . It is demonstrated that the tested phosphonates iminodi(methylenephosphonic acid) (IDMP), hydroxyethelidene(diphosphonic acid) (HEDP), aminotris(methylenephosphonic acid) (ATMP), ethylenediaminetetra(methyloenephosphonic acid) (EDTMP) and diethylenetriaminepenta(methylenephosphonic acid) (DTPMP) dissolved in tap water are not detectable by LC/MS without sample clean-up. Only the smallest aminophosphonate amino(methylenephosphonic acid) (AMPA) was detectable but the recovery is decreased drastically. The optimised sample clean-up with cation exchange resin (CER) Dowex 50WX8 is described in detail and illustrated. The protocol is provided. The influence of the incubation time, addition of different ammonium acetate concentrations, different samples pHs and different water qualities is demonstrated and preferred clean- up conditions are recommended. Calibration results of all tested aminophosphonates are validated regarding limit of detection, limit of quantification, lower limit of quantification, absolute and relative process standard deviation. A final recommendation for the best clean-up condition for all six tested aminophosphonates is provided. KW - Phosphonates KW - LC/MS analysis KW - Cation Exchange Resin KW - Clean-up Y1 - 2020 U6 - https://doi.org/10.1016/j.mex.2020.100933 SN - 2215-0161 VL - 07 SP - 1 EP - 11 ER - TY - THES A1 - Kalbfleisch, Katrin T1 - Synthese sowie Homo- und Copolymerisation von tert.-Butylaminomethylstryrol Y1 - 2018 CY - Cottbus ER - TY - CHAP A1 - Schirmer, Mario A1 - Martienssen, Marion ED - Barceló, Damià T1 - Enhanced Natural Attenuation of MTBE T2 - Fuel oxygenates Y1 - 2007 SN - 978-3-540-72640-1 SN - 978-3-540-72641-8 SP - 139 EP - 158 PB - Springer CY - Berlin [u.a.] ER - TY - GEN A1 - Martienssen, Marion A1 - Naumann, R. T1 - Änderungen der Belebtschlammzusammensetzung einer Industriekläranlage unter dem Einfluß von Inhibitoren der Stickstoffelimination T2 - Korrespondenz Abwasser Y1 - 2000 SN - 1616-430X VL - 47 IS - 8 SP - 1151 EP - 1157 ER - TY - GEN A1 - Martienssen, Marion T1 - Simultaneous catalytic detoxification and biodegradation of organic peroxides during the biofilm process Y1 - 2000 ER - TY - GEN A1 - Martienssen, Marion A1 - Reichel, O. A1 - Kohlweyer, U. T1 - Surface properties of bacteria from different waste water treatment plants T2 - Acta Biotechnologica Y1 - 2001 VL - 21 IS - 3 SP - 207 EP - 225 ER - TY - PAT A1 - Martienssen, Marion A1 - Rohwerder, Thore A1 - Müller, Roland H. T1 - Verfahren für den vollständigen biologischen Abbau von MTBE und TBA in Anweisenheit weiterer organischer Schadstoffe mittels trägerfixierter Mikroorganismen Y1 - 2006 ER - TY - GEN A1 - Böllmann, Jörg A1 - Scholler, Markus T1 - Glechoma hederacea (Lamiaceae) in North America: invasion history and current distribution T2 - Feddes Repertorium Y1 - 2004 U6 - https://doi.org/10.1002/fedr.200311035 SN - 0014-8962 SN - 1522-239X VL - 115 IS - 1-2 SP - 178 EP - 188 ER - TY - GEN A1 - Martienssen, Marion A1 - Reichel, O. A1 - Schirmer, M. T1 - Einsatz oberflächenaktiver Substanzen zur Verbesserung der biologischen Abbaubarkeit von Mineralölkohlenwasserstoffen T2 - Chemie-Ingenieur-Technik Y1 - 2003 SN - 0009-286X VL - 75 IS - 11 SP - 1749 EP - 1755 ER - TY - GEN A1 - Martienssen, Marion A1 - Weiß, H. A1 - Hasselwander, E. A1 - Schmid, J. A1 - Schirmer, M. T1 - Natürlicher Abbau von MTBE im Grundwasser - Großversuch am Standort Leuna T2 - Altlastenspektrum Y1 - 2003 SN - 0942-3818 VL - 12 IS - 4 SP - 173 EP - 179 ER - TY - GEN A1 - Martienssen, Marion A1 - Gründl, Marlies T1 - Immobilized microorganisms for the degradation of PAH and hydrocarbons from contaminated soil Y1 - 2000 ER - TY - GEN A1 - Martienssen, Marion A1 - Gründl, Marlies T1 - Einsatz trägerfixierter Mikroorganismen zur Eliminierung von PAH und Mineralölkohlenwasserstoffen aus kontaminierten Böden T2 - Chemie-Ingenieur-Technik Y1 - 1999 SN - 0009-286X VL - 71 IS - 6 SP - 624 EP - 628 ER - TY - PAT A1 - Martienssen, Marion T1 - Verfahren zur mechanisch-mikribiologischen Reinigung kontaminierter Böden Y1 - 1999 ER - TY - GEN A1 - Jank, M. A1 - Köser, H. A1 - Lücking, F. A1 - Martienssen, Marion A1 - Wittich, S. T1 - Decolorization and degradation of Erioglaucine (Acid Blue 9) dye in wastewater Y1 - 1998 ER - TY - GEN A1 - Martienssen, Marion A1 - Budahn, G. A1 - Gründl, Marlies T1 - Mikroorganismen im Waschprozeß T2 - UmweltMagazin Y1 - 1998 SN - 0173-363X VL - 27 IS - 9 SP - 42 EP - 43 ER - TY - GEN A1 - Martienssen, Marion A1 - Simon, J. A1 - Schulze, R. T1 - Untersuchungen zum Einsatz von aufbereiteter Kfz-Kühlerflüssigkeit als organische C-Quelle bei der biologischen Sickerwasserbehandlung T2 - Korrespondenz Abwasser Y1 - 1997 SN - 0341-1540 VL - 44 IS - 9 SP - 1622 EP - 1627 ER - TY - GEN A1 - Martienssen, Marion A1 - Schübb, S. T1 - Der Einfluß geladener Oberflächen bei der Anreicherung und Selektion von Mikroorganismen für den Abbau methylierter Phenole T2 - GWF : Wasser, Abwasser Y1 - 1998 VL - 139 IS - 4 SP - 214 EP - 219 ER - TY - GEN A1 - Martienssen, Marion A1 - Simon, J. T1 - Reinigung von Abwasser aus Emulsionsspaltanlagen durch kombinierten Einsatz von biologischer Behandlung und chemischer Naßoxidation T2 - Korespondenz Abwasser Y1 - 1997 SN - 0341-1540 VL - 44 IS - 1 SP - 110 EP - 114 ER - TY - GEN A1 - Becker, M. A1 - Martienssen, Marion A1 - Fuhrmann, Bernd A1 - Spohn, U. T1 - Analysing nitrogen and sulphide elimination from leachate by coulometric continuous flow titration Y1 - 1997 ER - TY - GEN A1 - Martienssen, Marion A1 - Schöps, R. T1 - Biological treatment of leachate from solid waste landfill sites - Alterations in the bacterial community during the denitrification process Y1 - 1997 ER - TY - GEN A1 - Martienssen, Marion A1 - Simon, J. T1 - Effect of activated carbon on the biological treatment of oil-water emulsions Y1 - 1996 ER - TY - CHAP A1 - Becker, M. A1 - Spohn, U. A1 - Fuhrmann, Bernd A1 - Martienssen, Marion T1 - Überwachung der Nitrifikation von Deponiesickerwässern durch 223 computergesteuerte coulometrische Durchflußtitrationmitbiamperometrischer oder chemiluminometrischer Endpunktindikation T2 - Technische Systeme für die Biotechnologie und Umwelt, 7. Heiligenstädter Kolloquium, 7.11. - 9.11. 1994 Y1 - 1994 SP - 223 EP - 231 CY - Heiligenstadt ER - TY - CHAP A1 - Martienssen, Marion T1 - Biological treatment of leachate from solid waste landfill sites - the influence of the activated sludge composition to the denitrification process T2 - Biochemical engineering 3, Third International Symposium on Biochemical Engineering, Stuttgart, Germany, March 6 - 8, 1995 Y1 - 1995 SN - 3-00-000360-6 SP - 135 EP - 137 PB - Universität CY - Stuttgart ER - TY - GEN A1 - Martienssen, Marion A1 - Schulze, R. A1 - Simon, J. T1 - Capacities and limits of three different technologies for the biological treatment of leachate from solid waste landfill sites Y1 - 1995 ER - TY - PAT A1 - Martienssen, Marion A1 - Schulze, Rolf A1 - Simon, Jürgen T1 - Verfahren zur Stabilisierung der Denitrifikation in biologischen Kläranlagen durch verbrauchte Kfz-Kühlerflüssigkeit Y1 - 1995 ER - TY - CHAP A1 - Martienssen, Marion A1 - Schulze, R. A1 - Heinke, M. A1 - Pohl, W. A1 - Simon, J. T1 - Leistungen und Leistungsgrenzen unterschiedlicher biotechnologischer Verfahren für die Behandlung von Deponiesickerwasser T2 - 11. Jahrestagung der Biotechnologen, Nürnberg, 24. bis 26. Mai 1993, Kurzfassungen Y1 - 1993 SP - 43 EP - 44 PB - DECHEMA CY - Frankfurt am Main ER - TY - GEN A1 - Kutscherra, M. A1 - Jost, W. A1 - Schlee, D. T1 - Isoenzymes of Nitrite Reduktase in higher plants-occurrence, purification and alteration during ontogenesis Y1 - 1987 ER - TY - THES A1 - Engelbrecht, Steffen T1 - Mikrobielle Stickstoffelimination mit kombinierter partieller Nitrifikation / Anammox (PN/A) in membranbelüfteten hochsalinen und Niedrigtemperatur-Festbettreaktoren N2 - Durch anthropogene Aktivität hat sich der Nährstoffeintrag in die Umwelt stark erhöht. Die Hauptquellen sind die Landwirtschaft, kommunales und industrielles Abwasser. Erhöhte Stickstofffrachten haben einen starken Einfluss auf den Stickstoffkreislauf. Das Überangebot an Stickstoff führt zu Eutrophierung in Gewässern und somit zu einem weniger diversen, instabileren Ökosystem. Die Stickstoffeliminierung aus Abwässern ist essentiell für den Schutz der Ökosysteme. Das etablierte System der Nitrifikation/Denitrifikation ist aufgrund des hohen Sauerstoffbedarfs sehr energie- und somit auch kostenintensiv. Der Anammox-Prozess (anaerobe Ammonium Oxidation) ist eine potentielle Alternative zur konventionellen Stickstoffelimination. Anammox-Bakterien nutzen Ammonium und Nitrit als Elektronenakzeptor. Durch Nutzung der partiellen Nitrifikation in Kombination mit Anammox (PN/A) kann bis zu 60 % der Belüftungsenergie eingespart werden. Trotz Erfolgen in der Teilstrombehandlung ist der Prozess noch nicht erfolgreich in der Hauptstrombehandlung eingesetzt worden. Die größten Herausforderungen sind die niedrigen Temperaturen, geringe Zulaufkonzentrationen und hohe Konzentrationen organischen Kohlenstoffs. Die vorliegende Arbeit konnte die Wirksamkeit von membranbelüfteten Biofilm Reaktoren unter Verwendung von synthetischem Abwasser bei niedrigen Temperaturen (18 - 8 °C) belegen. Die Innovation in diesem System ist die räumliche Trennung von nitrifizierenden Bakterien und Anammox Bakterien. Die regelmäßige mechanische Entfernung des Nitrifikanten-Biofilms ermöglichte einen langzeitstabilen Reaktorbetrieb. Diese Reaktorkonfiguration konnte auch erfolgreich für hochsaline Abwässer (5 -30 g NaCl*L-1) bei einer Reaktortemperatur von 30 °C und auch unter Zugabe von TOC (40-100 mg TOC*L-1) genutzt werden. Weiterhin wurden Untersuchungen zur Aktivität von salzadaptierter Anammox-Biomasse und nicht adaptierter Biomasse in einem Bereich von 0 bis 50 g NaCl*L-1 durchgeführt. Bei Konzentrationen unter 30 g NaCl*L-1 konnten keine signifikanten Unterschiede im Abbauverhalten festgestellt werden. Allerdings war adaptierte Biomasse bei höheren Salzkonzentrationen konkurrenzstärker. Außerdem wurden Langzeitlagerungsversuche durchgeführt. Die Lagerungsbedingungen variierten in der Lagerungstemperatur, im Redoxpuffer und der Nährstoffversorgung. Nach 106 Tagen bei 4 °C konnte die Anammox-Biomasse noch 91 % ihrer ursprünglichen Aktivität erhalten. Somit konnte eine einfache und kostengünstige Lagerungsmethode für Anammo-Bakterien entwickelt werden. Y1 - 2018 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:co1-opus4-48848 CY - Cottbus ER - TY - GEN A1 - Rathsack, Kristina A1 - Reitner, Joachim A1 - Stackebrandt, E. A1 - Tindall, B. J. T1 - Reclassification of Aurantimonas altamirensis (Jurado et al. 2006), Aurantimonas ureilytica (Weon et al. 2007) and Aurantimonas frigidaquae (Kim et al. 2008) as members of a new genus, Aureimonas gen. nov., as Aureimonas altamirensis gen. nov., comb. nov., Aureimonas ureilytica comb. nov. and Aureimonas frigidaquae comb. nov., and emended descriptions of the genera Aurantimonas and Fulvimarina T2 - International journal of systematic and evolutionary microbiology : IJSEM Y1 - 2011 SN - 1466-5034 VL - 61 IS - 11 SP - 2722 EP - 2728 ER - TY - GEN A1 - Rathsack, Kristina A1 - Rathsack, Udo T1 - Besiedlung von Wasserversorgungsnetzen durch Asseln und andere Invertebraten : Mess- und Beobachtungsverfahren T2 - GWF : Wasser, Abwasser Y1 - 2011 SN - 0016-3651 VL - 152 IS - 4 SP - 390 EP - 396 ER - TY - CHAP A1 - Rathsack, Kristina A1 - Quéric, Nadia-Valérie A1 - Reitner, Joachim ED - Reitner, Joachim T1 - Deep biosphere of the oceanic deep sea T2 - Encyclopedia of Geobiology Y1 - 2011 SN - 978-1-4020-9211-4 SP - 317 EP - 322 PB - Springer CY - Dordrecht ER - TY - PAT A1 - Martienssen, Marion T1 - Verfahren zur Verringerung der Bildung von Biofilmen und Schwefelwassestoff in Abwasserkanälen Y1 - 2011 ER - TY - GEN A1 - Rathsack, Kristina T1 - Besiedlung von Wasserversorgungssystemen durch Invertebraten T2 - Energie-, Wasser-Praxis Y1 - 2012 SN - 1436-6134 IS - 6 SP - 28 EP - 33 ER - TY - CHAP A1 - Krieg, Ronald A1 - Martienssen, Marion T1 - Einfluss von Tränkezusätzen auf die Wasseraufnahme bei Kaninchen und die Biofilmbildung T2 - 17. Internationale Tagung über Haltung und Krankheiten der Kaninchen, Pelztiere und Heimtiere T2 - 17. Symposium on Housing and Deaseases of Rabbits, Furproviding Animals and Pet Animals Y1 - 2011 SN - 978-3-8359-5809-8 SP - 178 EP - 189 PB - VVB Laufersweiler Verlag ER - TY - GEN A1 - Martienssen, Marion A1 - Schulze, Ralf T1 - Lanzeituntersuchungen zum Einsatz von Rekultivierungs-/Methanoxidationsschichten auf Hausmülldeponien T2 - Müll und Abfall Y1 - 2012 SN - 1863-9763 SN - 0027-2957 VL - 44 IS - 4 SP - 202 EP - 209 ER - TY - GEN A1 - Kuhn, Ramona A1 - Benndorf, Dirk A1 - Rapp, Erdmann A1 - Reichl, Udo A1 - Palese, Luigi Leonardo A1 - Pollice, Alfieri T1 - Metaproteome analysis of sewage sludge from membrane bioreactors T2 - Proteomics N2 - Microbial dynamics and enzymatic activities of activated sludge processes are not completely understood yet. A better understanding about the biology is indispensable for further process optimization. Since proteins play a key role as catalysts in sludge processes, a protocol for protein extraction and analysis by 2-D PAGE was established. It is based on phenol extraction of alkaline extracts and on a subsequent precipitation with ammonium sulphate. 2-D protein patterns obtained from different sludges collected from membrane bioreactors showed – besides common spots – significant differences. Selected proteins were identified with nano- HPLC-ESI-MS/MS. All membrane biological reactor (MBR) sludge samples investigated in this study contained elastase 3A, which implies that this human serine protease is a significant constituent of municipal wastewater. Although the identification of proteins from ammonia-oxidizing bacterium Nitrosomonas europaea was expected, the detection of a protein with homology to the marine bacterium Saprospira grandis in MBR1 was surprising. KW - De novo sequencing KW - Membrane bioreactor KW - metaproteomics KW - microbial communities KW - Microbiology KW - Protein extraction Y1 - 2011 U6 - https://doi.org/10.1002/pmic.201000590 SN - 1615-9861 VL - 11 IS - 13 SP - 2738 EP - 2744 ER - TY - CHAP A1 - Schirmer, Mario A1 - Reinstorf, Frido A1 - Leschik, Sebastian A1 - Musolff, Andreas A1 - Krieg, Ronald A1 - Osenbrück, Karsten A1 - Martienssen, Marion A1 - Schirmer, Kirsten A1 - Strauch, Gerhard ED - Fatta-Kassinos, Despo T1 - Transport and Fate of Xenobiotics in the Urban Water Cycle: Studies in Halle/Saale and Leipzig (Germany) T2 - Xenobiotics in the Urban Water Cycle : mass flows, environmental processes, mitigation and treatment strategies Y1 - 2010 SN - 978-90-481-3508-0 U6 - https://doi.org/10.1007/978-90-481-3509-7_12 SP - 213 EP - 226 PB - Springer CY - Dordrecht [u.a.] ER - TY - GEN A1 - Martienssen, Marion A1 - Reichel, O. A1 - Schirmer, M. T1 - Use of surfactants to improve the biological degradability of petroleum hydrocarbons Y1 - 2007 ER - TY - CHAP A1 - Martienssen, Marion ED - Bastiaens, Leen T1 - Comparison of different technologies for the treatment of MTBE contaminated groundwater T2 - MTBE and other fuel oxygenates, Proceedings of the 3rd European conference, Antwerp, 7-8 June, 2007 Y1 - 2007 SP - 57 EP - 62 PB - VITO CY - Mol ER - TY - CHAP A1 - Beckmann, Annika A1 - Zittwitz, Mark A1 - Gerhardt, Matthias A1 - Martienssen, Marion A1 - Geistlinger, Helmut A1 - Schirmer, Mario T1 - Modellgestützte Feedback-Steuerung für modulare in-situ Gaswände T2 - Behandlungstechnologien für bergbaubeeinflusste Wässer/GIS - Geowissenschaftliche Anwendungen und Entwicklungen, Proceedingsband zu den Workshops am Geologischen Institut der TU Bergakademie Freiberg am 17. Juni 2005 Y1 - 2005 SP - 9 EP - 13 PB - Inst. für Geologie CY - Freiberg ER - TY - CHAP A1 - Böllmann, Jörg A1 - Scholler, Markus T1 - Studies in the life cycle of Puccinia glechomatis T2 - The 7th International Mycological Congress, Oslo, 2002 : Book of abstracts Y1 - 2002 UR - http://www.ima-mycology.org/sites/default/files/downloads/imc7_book_of_abstracts.pdf SP - S. 281 PB - Congress Secretariat CY - Oslo ER - TY - GEN A1 - Kuhn, Ramona A1 - Jensch, Robert A1 - Fischer, Thomas A1 - Keuler, Klaus A1 - Bryant, Isaac Mbir A1 - Martienssen, Marion T1 - Sunlight Degradation of the Aminophosphonate Diethylenetriamine Penta-(Methylenephosphonic Acid) T2 - Solar N2 - Aminophosphonate diethylenetriamine penta(methylenephosphonic acid) (DTPMP) is a scale inhibitor commonly used in several industries. DTPMP is suspected to cause anthropogenic pollution through discharge into the aquatic environment. DTPMP is assumed to be degraded by sunlight radiation. We recently predicted a preliminary degradation pathway of DTPMP applyingUV treatment. Currently, we have not yet evidenced that DTPMP shows the same degradation pattern with natural sunlight. One major reason leads to the fact that the light spectrum emitted by UV lamps does not completely represent the natural sunlight spectrum, and the emitted UVB and UVA irradiation flux is much higher than for solar light. For that reason, the degradation pattern and kinetics might be different between artificial UV treatment and natural sunlight treatment. Here, we investigated whether DTPMP is degradable under natural sunlight radiation, and whether the degradation mechanisms determined through UV treatment are transferable to sunlight. We investigated five different treatment conditions, i.e., DTPMP degradation in direct or diffuse sunlight, in diffuse sunlight with addition of Ca2+ or Mg2+, and in diffuse sunlight with local TW. Our experiment was carried out from March 2021 to October 2021. We performed LC/MS analyses and measured the release of o-PO4 3  . DTPMP was degraded with all five treatment conditions. The fastest DTPMP degradation occurred in direct and diffuse sunlight without addition of bivalent cations. The addition of Ca2+ and Mg2+ resulted in inhibited degradation. Similar effects occurred for sunlight treatment with local TW. We evidenced different degradation mechanisms for DTPMP depending on the presence of alkaline earth metals as we previously proposed for UV-treated DTPMP. However, both degradation mechanisms of DTPMP belong to the same degradation pathway determined with UV treatment. Therefore, we conclude that DTPMP undergoes a similar degradation pathway in sunlight as compared to UV light. KW - Photochemistry Y1 - 2022 U6 - https://doi.org/10.3390/solar2020009 SN - 2673-9941 VL - 2 IS - 2 SP - 141 EP - 157 ER - TY - GEN A1 - Okofo, Louis Boansi A1 - Bedu-Addo, Kenneth A1 - Martienssen, Marion T1 - Characterization of groundwater in the "Tamnean" Plutonic Suiteaquifers unsing hydrogeochemical and multivariate statistical evidence:a study in the Garu-Tempane District, Upper East Region of Ghana T2 - Applied Water Science Y1 - 2022 U6 - https://doi.org/10.1007/s13201-021-01559-2 SN - 2190-5495 VL - 12 IS - 2 ER - TY - GEN A1 - Rathsack, Kristina T1 - Unerwünschte Bewohner : Wasserasseln und andere Makroorganismen in Trinkwasserversorgungsnetzen T2 - IKZ-Fachplaner : Magazin für Planer, Berater und Entscheider der Gebäudetechnik Y1 - 2012 VL - 7 IS - 11 SP - 8 EP - 12 ER - TY - CHAP A1 - Böllmann, Jörg A1 - Martienssen, Marion A1 - Nixdorf, Brigitte T1 - Stickstoffumsätze in unterschiedlichen Gewässertypen Berlin/Brandenburgs, Teil 1: Zellzahlen von Nitrifikanten und Denitrifikanten T2 - Erweiterte Zusammenfassung der Jahrestagung der DGL 2011 in Weihenstephan KW - Nitrifikation KW - Stoffumsätze Y1 - 2012 SN - 978-3-9813095-2-2 SP - 234 EP - 238 PB - Eigenverlag der DGL CY - Hardegsen ER - TY - GEN A1 - Martienssen, Marion A1 - Schulze, Rolf T1 - Langzeituntersuchungen zum Einsatz von Rekultivierungs-/Methanoxidationsschichten auf Hausmülldeponien T2 - Müll und Abfall Y1 - 2012 SN - 1863-9763 SN - 0027-2957 VL - 44 IS - 4 SP - 202 EP - 209 ER - TY - THES A1 - Ebert, Sebastian T1 - Potentiale und Grenzen eines neuen Verfahrens zum linienhaften Gasmonitoring in Böden Y1 - 2012 CY - Cottbus ER - TY - GEN A1 - Sarpong, Daniella A1 - Amankwaa, Gordon A1 - Martienssen, Marion A1 - Burkhardt, Marko T1 - A novel biomethane (BMP) and somposting (CMP) potential framework for determining biogas and composting potential of urban organic waste T2 - Environmental Technology Y1 - 2022 U6 - https://doi.org/10.1080/09593330.2022.2145241 SN - 1479-487X VL - 45(2024) IS - 8 SP - 1471 EP - 1482 ER - TY - GEN A1 - Riedel, Ramona A1 - Commichau, Fabian M. A1 - Benndorf, Dirk A1 - Hertel, Robert A1 - Holzer, Katharina A1 - Mardoukhi, Mohammad Saba Yousef A1 - Noack, Laura A1 - Martienssen, Marion T1 - Biodegradation of selected aminophosphonates by the bacterial isolate Ochrobactrum sp. BTU1 T2 - Microbial Research N2 - Aminophosphonates, like glyphosate (GS) or metal chelators such as ethylenediaminetetra(methylenephosphonic acid) (EDTMP), are released on a large scale worldwide. Here, we have characterized a bacterial strain capable of degrading synthetic aminophosphonates. The strain was isolated from LC/MS standard solution. Genome sequencing indicated that the strain belongs to the genus Ochrobactrum. Whole-genome classification using pyANI software to compute a pairwise ANI and other metrics between Brucella assemblies and Ochrobactrum contigs revealed that the bacterial strain is designated as Ochrobactrum sp. BTU1. Degradation batch tests with Ochrobactrum sp. BTU1 and the selected aminophosphonates GS, EDTMP, aminomethylphosphonic acid (AMPA), iminodi(methylene-phosphonic) (IDMP) and ethylaminobis(methylenephosphonic) acid (EABMP) showed that the strain can use all phosphonates as sole phosphorus source during phosphorus starvation. The highest growth rate was achieved with AMPA, while EDTMP and GS were least supportive for growth. Proteome analysis revealed that GS degradation is promoted by C-P lyase via the sarcosine pathway, i.e., initial cleavage at the C-P bond. We also identified C-P lyase to be responsible for degradation of EDTMP, EABMP, IDMP and AMPA. However, the identification of the metabolite ethylenediaminetri(methylenephosphonic acid) via LC/MS analysis in the test medium during EDTMP degradation indicates a different initial cleavage step as compared to GS. For EDTMP, it is evident that the initial cleavage occurs at the C-N bond. The detection of different key enzymes at regulated levels, form the bacterial proteoms during EDTMP exposure, further supports this finding. Y1 - 2024 U6 - https://doi.org/10.1016/j.micres.2024.127600 SN - 0944-5013 VL - 280 SP - 1 EP - 12 ER - TY - GEN A1 - Hertel, Robert A1 - Schöne, Kerstin A1 - Mittelstädt, Carolin A1 - Meißner, Janek A1 - Zschoche, Nick A1 - Collignon, Madeline A1 - Kohler, Christian A1 - Friedrich, Ines A1 - Schneider, Dominik A1 - Hoppert, Michael A1 - Kuhn, Ramona A1 - Schwedt, Inge A1 - Scholz, Patricia A1 - Poehlein, Anja A1 - Martienssen, Marion A1 - Ischebeck, Till A1 - Daniel, Rolf A1 - Commichau, Fabian M. T1 - Characterization of glyphosate-resistant Burkholderia anthina and Burkholderia cenocepacia isolates from a commercial Roundup® solution T2 - Environmental Microbiology Reports N2 - Roundup® is the brand name for herbicide solutions containing glyphosate, which specifically inhibits the 5-enolpyruvyl-shikimate-3-phosphate (EPSP) synthase of the shikimate pathway. The inhibition of the EPSP synthase causes plant death because EPSP is required for biosynthesis of aromatic amino acids. Glyphosate also inhibits the growth of archaea, bacteria, Apicomplexa, algae and fungi possessing an EPSP synthase. Here, we have characterized two glyphosate-resistant bacteria from a Roundup solution. Taxonomic classification revealed that the isolates 1CH1 and 2CH1 are Burkholderia anthina and Burkholderia cenocepacia strains respectively. Both isolates cannot utilize glyphosate as a source of phosphorus and synthesize glyphosate-sensitive EPSP synthase variants. Burkholderia. anthina 1CH1 and B. cenocepacia 2CH1 tolerate high levels of glyphosate because the herbicide is not taken up by the bacteria. Previously, it has been observed that the exposure of soil bacteria to herbicides like glyphosate promotes the development of antibiotic resistances. Antibiotic sensitivity testing revealed that the only the B. cenocepacia 2CH1 isolate showed increased resistance to a variety of antibiotics. Thus, the adaptation of B. anthina 1CH1 and B. cenocepacia 2CH1 to glyphosate did not generally increase the antibiotic resistance of both bacteria. However, our study confirms the genomic adaptability of bacteria belonging to the genus Burkholderia. Y1 - 2022 U6 - https://doi.org/10.1111/1758-2229.13022 SN - 1758-2229 VL - 14 IS - 1 SP - 70 EP - 84 ER - TY - GEN A1 - Hertel, Robert A1 - Gibhardt, Johannes A1 - Martienssen, Marion A1 - Kuhn, Ramona A1 - Commichau, Fabian M. T1 - Molecular mechanisms underlying glyphosate resistance in bacteria T2 - Environmental Microbiology N2 - Glyphosate is a nonselective herbicide that kills weeds and other plants competing with crops. Glyphosate specifically inhibits the 5-enolpyruvyl-shikimate-3-phosphate (EPSP) synthase, thereby depleting the cell of EPSP serving as a precursor for biosynthesis of aromatic amino acids. Glyphosate is considered to be toxicologically safe for animals and humans. Therefore, it became the most-important herbicide in agriculture. However, its intensive application in agriculture is a serious environmental issue because it may negatively affect the biodiversity. A few years after the discovery of the mode of action of glyphosate, it has been observed that bacteria evolve glyphosate resistance by acquiring mutations in the EPSP synthase gene, rendering the encoded enzyme less sensitive to the herbicide. The identification of glyphosate-resistant EPSP synthase variants paved the way for engineering crops tolerating increased amounts of the herbicide. This review intends to summarize the molecular mechanisms underlying glyphosate resistance in bacteria. Bacteria can evolve glyphosate resistance by (i) reducing glyphosate sensitivity or elevating production of the EPSP synthase, by (ii) degrading or (iii) detoxifying glyphosate and by (iv) decreasing the uptake or increasing the export of the herbicide. The variety of glyphosate resistance mechanisms illustrates the adaptability of bacteria to anthropogenic substances due to genomic alterations. Y1 - 2021 U6 - https://doi.org/10.1111/1462-2920.15534 SN - 1462-2920 SN - 1462-2912 VL - 23 IS - 6 SP - 2891 EP - 2905 ER - TY - GEN A1 - Ahmed, Naveed A1 - Martienssen, Marion A1 - Bryant, Isaac Mbir A1 - Vione, Davide A1 - Bruzzoniti, Maria Concetta A1 - Riedel, Ramona T1 - Investigation on UV Degradation and Mechanism of 6:2 Fluorotelomer Sulfonamide Alkyl Betaine, Based on Model Compound Perfluorooctanoic Acid T2 - ChemEngineering N2 - The UV treatment of 6:2 FTAB involves the mitigation of this persistent chemical by the impact of ultraviolet radiation, which is known for its resistance to environmental breakdown. UV treatment of PFOA and/or 6:2 FTAB, and the role of responsible species and their mechanism have been presented. Our investigation focused on the degradation of perfluorooctanoic acid (PFOA) and 6:2 fluorotelomer sulfonamide alkyl betaine (6:2 FTAB, Capstone B), using UV photolysis under various pH conditions. Initially, we used PFOA as a reference, finding a 90% decomposition after 360 min at the original (unadjusted) pH 5.6, with a decomposition rate constant of (1.08 ± 0.30) × 10−4 sec−1 and a half-life of 107 ± 2 min. At pH 4 and 7, degradation averaged 85% and 80%, respectively, while at pH 10, it reduced to 57%. For 6:2 FTAB at its natural pH 6.5, almost complete decomposition occurred. The primary UV transformation product was identified as 6:2 fluorotelomer sulfonic acid (6:2 FTSA), occasionally accompanied by shorter-chain perfluoroalkyl acids (PFAAs) including PFHpA, PFHxA, and PFPeA. Interestingly, the overall decomposition percentages were unaffected by pH for 6:2 FTAB, though pH influenced rate constants and half-lives. In PFOA degradation, direct photolysis and reaction with hydrated electrons were presumed mechanisms, excluding the involvement of hydroxyl radicals. The role of superoxide radicals remains uncertain. For 6:2 FTAB, both direct and indirect photolysis were observed, with potential involvement of hydroxyl, superoxide radicals, and/or other reactive oxygen species (ROS). Clarification is needed regarding the role of 𝑒−𝑎𝑞 in the degradation of 6:2 FTAB. KW - PFOA KW - 6:2 FTAB KW - decomposition KW - photolysis KW - scavenger Y1 - 2024 U6 - https://doi.org/10.3390/chemengineering8020032 SN - 2305-7084 VL - 8 IS - 2 ER - TY - BOOK A1 - Hoffmann, Melanie A1 - Anders, Kenneth A1 - Fischer, Lars A1 - Böllmann, Jörg A1 - Brückner, Ardo A1 - Brunk, Ingo A1 - Donath, Helmut A1 - Donat, Ralf A1 - Elmer, Michael A1 - Güth, Mareike A1 - Häußler, Robert A1 - Mrzljak, Jadranka A1 - Mudra, Dieter A1 - Müller, Markus A1 - Peschel, Tim A1 - Pilz, Wilfried A1 - Sawade, Wolfgang A1 - Schonert, P. A1 - Siedschlag, Marina A1 - Siedschlag, Yvonne A1 - Wanner, Manfred A1 - Wöllecke, Jens ED - Hoffmann, Melanie ED - Anders, Kenneth ED - Fischer, Lars T1 - Kolonisten gesucht! Steckbriefe natürlicher und sozialer Besiedler der Bergbaufolgelandschaft Schlabendorfer Felder Y1 - 2010 PB - Büro für Landschaftskommunikation CY - Bad Freienwalde ER - TY - CHAP A1 - Elmer, Michael A1 - Wöllecke, Jens A1 - Gebhardt, Sascha A1 - Böllmann, Jörg A1 - Hohberg, Karin A1 - Wanner, Manfred A1 - Xylander, Willi E. R. A1 - Hüttl, Reinhard F. ED - Hotes, Stefan ED - Wolters, Volkmar T1 - Primärsukzession der Bodenorganismen T2 - Fokus Biodiversität : wie Biodiversität in der Kulturlandschaft erhalten und nachhaltig genutzt werden kann Y1 - 2010 SN - 978-3-86581-172-1 SP - 125 EP - 131 PB - oekom CY - München ER - TY - GEN A1 - Okofo, Louis Boansi A1 - Adonadaga, Melvin-Guy A1 - Martienssen, Marion T1 - Groundwater age dating using multi-environmental tracers (SF6, CFC-11, CFC-12, δ18O, and δD) to investigate groundwater residence times and recharge processes in Northeastern Ghana T2 - Journal of Hydrology Y1 - 2022 U6 - https://doi.org/10.1016/j.jhydrol.2022.127821 SN - 1879-2707 SN - 0022-1694 VL - Vol. 610 SP - 1 EP - 18 ER - TY - GEN A1 - Ahmed, Naveed A1 - Martienssen, Marion A1 - Bryant, Isaac Mbir A1 - Vione, Davide A1 - Bruzzoniti, Maria Concetta A1 - Riedel, Ramona T1 - Investigation on UV Degradation and Mechanism of 6:2 Fluorotelomer Sulfonamide Alkyl Betaine, Based on Model Compound Perfluorooctanoic Acid T2 - ChemEngineering Y1 - 2024 U6 - https://doi.org/10.3390/chemengineering8020032 SN - 2305-7084 VL - 8 IS - 2 ER - TY - GEN A1 - Afitiri, Abdul-Rahaman A1 - Aram, Simon Appah A1 - Martienssen, Marion T1 - Sytematic review of the effects of Advanced Oxidation Processes integration with solar water disinfection for improved drinking water production T2 - Waste Management Bulletin Y1 - 2024 U6 - https://doi.org/10.1016/j.wmb.2023.08.005 SN - 2949-7507 VL - 1 IS - 4 SP - 52 EP - 59 ER - TY - GEN A1 - Böllmann, Jörg A1 - Martienssen, Marion T1 - Seasonal Variability of Cultivable Nitrate-Reducing and Denitrifying Beacteria and Functional Gene Copy Number in Fresh Water Lake T2 - Microorganisms Y1 - 2024 U6 - https://doi.org/10.3390/microorganisms12030511 SN - 2076-2607 VL - 12 IS - 3 ER - TY - GEN A1 - Böllmann, Jörg A1 - Elmer, Michael A1 - Wöllecke, Jens A1 - Raidl, Stefan A1 - Hüttl, Reinhard F. T1 - Defensive strategies of soil fungi to prevent grazing by Folsomia candida (Collembola) T2 - Pedobiologia Y1 - 2010 SN - 1873-1511 VL - 53 IS - 2 SP - 107 EP - 114 ER - TY - CHAP A1 - Wöllecke, Jens A1 - Böllmann, Jörg A1 - Schneider, Bernd Uwe A1 - Hüttl, Reinhard F. ED - Hüttl, Reinhard F. ED - Schneider, Bernd Uwe T1 - Vergleichende Untersuchungen zur Durchwurzelbarkeit und Mykorrhizierung auf forstlich rekultivierten Kippsubstraten T2 - Neue Ansätze in der land- und forstwirtschaftlichen Rekultivierung Y1 - 2009 SN - 978-3-937728-06-3 SN - 3-937728-06-6 SP - 207 EP - 224 PB - BTU CY - Cottbus ER -