TY - GEN A1 - Zhi, Zheng-liang A1 - Drazan, Viktor A1 - Wolfbeis, Otto S. A1 - Mirsky, Vladimir M. T1 - Electrocatalytic activity of DNA on electrodes as an indication of hybridization T2 - Bioelectrochemistry N2 - Electron transfer between metal electrodes and ferro/ferricyanide is completely suppressed at low ionic concentration. We describe here a new phenomenon related to this reaction: an immobilisation of thiolated single-stranded DNA on gold electrodes retains this activity at low ionic strength up to the level corresponding to the high ionic strength. In contrast, a hybridisation of the complementary DNAwith the thiolated single-stranded DNA followed by a binding onto the electrodes, attenuated the electrocatalytic effect. These effects can be used for discrimination between single-stranded DNA and double-stranded DNA and for semi-quantitative measurement of complementary DNA in a sample. KW - DNA KW - DNA-sensor KW - Biosensor KW - Electrocatalysi KW - Hybridisation KW - Oligonucleotide KW - Gene analysis Y1 - 2006 U6 - https://doi.org/10.1016/j.bioelechem.2005.02.006 SN - 1567-5394 VL - 68 IS - 1 SP - 1 EP - 6 ER -