TY - GEN A1 - Wrobel, Nadia A1 - Deininger, Werner A1 - Hegemann, Peter A1 - Mirsky, Vladimir M. T1 - Covalent immobilization of oligonucleotides on electrodes T2 - Colloids and Surfaces B: Biointerfaces N2 - 32P-labeled DNA oligomers were immobilized onto self-assembled monolayers formed by carboxy-modified alkylthiols on gold electrodes. The optimal conditions were evaluated by comparing of surface density of oligonucleotides measured for different immobilization conditions before and after desorption of physically adsorbed nucleotides. The results demonstrate that the physical adsorption depends strongly on immobilization conditions being typically 10 times higher than the chemical immobilization. Under optimal immobilization conditions, the surface density of oligonucleotides was more than 20% of the value calculated for a densely packed monolayer. KW - DNA KW - DNA immobilization KW - DNA sensor KW - Biosensor KW - Hybridization Y1 - 2003 U6 - https://doi.org/10.1016/S0927-7765(03)00155-3 SN - 0927-7765 VL - 32 IS - 2 SP - 157 EP - 162 ER -