TY - GEN A1 - Wrobel, Nadia A1 - Deininger, Werner A1 - Hegemann, Peter A1 - Mirsky, Vladimir M. T1 - Covalent immobilization of oligonucleotides on electrodes T2 - Colloids and Surfaces B: Biointerfaces N2 - 32P-labeled DNA oligomers were immobilized onto self-assembled monolayers formed by carboxy-modified alkylthiols on gold electrodes. The optimal conditions were evaluated by comparing of surface density of oligonucleotides measured for different immobilization conditions before and after desorption of physically adsorbed nucleotides. The results demonstrate that the physical adsorption depends strongly on immobilization conditions being typically 10 times higher than the chemical immobilization. Under optimal immobilization conditions, the surface density of oligonucleotides was more than 20% of the value calculated for a densely packed monolayer. KW - DNA KW - DNA immobilization KW - DNA sensor KW - Biosensor KW - Hybridization Y1 - 2003 U6 - https://doi.org/10.1016/S0927-7765(03)00155-3 SN - 0927-7765 VL - 32 IS - 2 SP - 157 EP - 162 ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Wrobel, Nadia A1 - Riepl, Michael A1 - Hirsch, Thomas A1 - Wolfbeis, Otto S. T1 - Intelligente Nanostrukturen für Biosensoren und Sensor-Arrays T2 - BioTec: Zeitschrift für Biotechnologie Y1 - 1999 SN - 0937-2725 VL - 10 SP - 28 EP - 32 ER - TY - CHAP A1 - Wrobel, Nadia A1 - Riepl, Michael A1 - Schinkinger, Manfred A1 - Mirsky, Vladimir M. A1 - Wolfbeis, Otto S. T1 - Capacitive thin film biosensors for bacteriophages and nucleic acids T2 - Proceedings, EMBEC ’99, November 4 - 7, 1999, Vienna, Austria Y1 - 1999 SP - 362 EP - 363 PB - IFMBE CY - Stevenage ER - TY - GEN A1 - Wrobel, Nadia A1 - Schinkinger, Manfred A1 - Mirsky, Vladimir M. T1 - A novel ultraviolet assay for testing side reactions of carbodiimides T2 - Analytical Biochemistry N2 - Carbodiimides possess considerable absorbance in the ultraviolet region; the extinction coefficient of 1-ethyl-(3-dimethylaminopropyl)carbodiimide (EDC) in water is ϵ214 = 6.3 · 103 L mol−1 cm−1. It provides a very simple method for testing possible side reactions of carbodiimides. This technique was used to study effects of pH, different buffers, and other components typically present in biological samples on EDC stability. It was shown that the hydrolysis rate in pure water increased from 1.5 · 10−5 to 5 · 10−4 s−1 by pH decreasing from 7 to 4. A strong increase of the rate of EDC loss was observed in the presence of different components. This effect can be described by the following row of hydrolysis rates: citrate ⪢ acetate ≈ phosphate ⪢ SDS. The results can be used to optimize carbodiimide-mediated reactions of peptide bond formation in organic chemistry or peptide synthesis or during immobilization or cross-linking of biological molecules. Y1 - 2002 U6 - https://doi.org/10.1006/abio.2002.5646 SN - 0003-2697 VL - 305 IS - 2 SP - 135 EP - 138 ER -