TY - GEN A1 - Ranjan, Amit A1 - Scholz, Julia A1 - Semmler, Torsten A1 - Wieler, Lothar H. A1 - Ewers, Christa A1 - Müller, Stefanie A1 - Pickard, Derek J. A1 - Schierack, Peter A1 - Tedin, Karsten A1 - Ahmed, Niyaz A1 - Schaufler, Katharina A1 - Guenther, Sebastian T1 - ESBL-plasmid carriage in E. coli enhances in vitro bacterial competition fitness and serum resistance in some strains of pandemic sequence types without overall fitness cost T2 - Gut pathogens Y1 - 2018 U6 - https://doi.org/10.1186/s13099-018-0243-z SN - 1757-4749 VL - 10 IS - 24 ER - TY - GEN A1 - Mohsin, Mashkoor A1 - Guenther, Sebastian A1 - Schierack, Peter A1 - Tedin, Karsten A1 - Wieler, Lothar H. T1 - Probiotic Escherichia coli Nissle 1917 reduces growth, Shiga toxin expression, release and thus cytotoxicity of enterohemorrhagic Escherichia coli T2 - International Journal of Medical Microbiology Y1 - 2015 U6 - https://doi.org/10.1016/j.ijmm.2014.10.003 SN - 1618-0607 SN - 1438-4221 VL - 305 IS - 1 SP - 20 EP - 26 ER - TY - GEN A1 - Frömmel, Ulrike A1 - Lehmann, Werner A1 - Rödiger, Stefan A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Weinreich, Jörg A1 - Groß, Julia A1 - Roggenbuck, Dirk A1 - Zinke, Olaf A1 - Ansorge, Hermann A1 - Vogel, Steffen A1 - Klemm, Per A1 - Wex, Thomas A1 - Schröder, Christian A1 - Wieler, Lothar H. A1 - Schierack, Peter T1 - Adhesion of human and animal Escherichia coli strains in association with their virulence-associated genes and phylogenetic origins T2 - Applied and environmental microbiology N2 - Intestinal colonization is influenced by the ability of the bacterium to inhabit a niche, which is based on the expression of colonization factors. Escherichia coli carries a broad range of virulence-associated genes (VAGs) which contribute to intestinal (inVAGs) and extraintestinal (exVAGs) infection. Moreover, initial evidence indicates that inVAGs and exVAGs support intestinal colonization. We developed new screening tools to genotypically and phenotypically characterize E. coli isolates originating in humans, domestic pigs, and 17 wild mammal and avian species. We analyzed 317 isolates for the occurrence of 44 VAGs using a novel multiplex PCR microbead assay (MPMA) and for adhesion to four epithelial cell lines using a new adhesion assay. We correlated data for the definition of new adhesion genes. inVAGs were identified only sporadically, particularly in roe deer (Capreolus capreolus) and the European hedgehog ( Erinaceus europaeus). The prevalence of exVAGs depended on isolation from a specific host. Human uropathogenic E. coli isolates carried exVAGs with the highest prevalence, followed by badger (Meles meles) and roe deer isolates. Adhesion was found to be very diverse. Adhesion was specific to cells, host, and tissue, though it was also unspecific. Occurrence of the following VAGs was associated with a higher rate of adhesion to one or more cell lines: afa-dra, daaD, tsh, vat, ibeA, fyuA, mat, sfa-foc, malX, pic, irp2, and papC. In summary, we established new screening methods which enabled us to characterize large numbers of E. coli isolates. We defined reservoirs for potential pathogenic E. coli. We also identified a very broad range of colonization strategies and defined potential new adhesion genes. Y1 - 2013 U6 - https://doi.org/10.1128/AEM.01384-13 SN - 1098-5336 SN - 0099-2240 VL - 79 IS - 19 SP - 5814 EP - 5829 ER - TY - GEN A1 - Schierack, Peter A1 - Rödiger, Stefan A1 - Kuhl, Christoph A1 - Hiemann, Rico A1 - Roggenbuck, Dirk A1 - Li, Ganwu A1 - Weinreich, Jörg A1 - Berger, Enrico A1 - Nolan, Lisa K. A1 - Nicholson, Bryon A1 - Römer, Antje A1 - Frömmel, Ulrike A1 - Wieler, Lothar H. A1 - Schröder, Christian T1 - Porcine E. coli: Virulence-Associated Genes, Resistance Genes and Adhesion and Probiotic Activity Tested by a New Screening Method T2 - PLoS One N2 - We established an automated screening method to characterize adhesion of Escherichia coli to intestinal porcine epithelial cells (IPEC-J2) and their probiotic activity against infection by enteropathogenic E. coli (EPEC). 104 intestinal E. coli isolates from domestic pigs were tested by PCR for the occurrence of virulence-associated genes, genes coding for resistances to antimicrobial agents and metals, and for phylogenetic origin by PCR. Adhesion rates and probiotic activity were examined for correlation with the presence of these genes. Finally, data were compared with those from 93 E. coli isolates from wild boars. Isolates from domestic pigs carried a broad variety of all tested genes and showed great diversity in gene patterns. Adhesions varied with a maximum of 18.3 or 24.2 mean bacteria adherence per epithelial cell after 2 or 6 hours respectively. Most isolates from domestic pigs and wild boars showed low adherence, with no correlation between adhesion/probiotic activity and E. coli genes or gene clusters. The gene sfa/foc, encoding for a subunit of F1C fimbriae did show a positive correlative association with adherence and probiotic activity; however E. coli isolates from wild boars with the sfa/foc gene showed less adhesion and probiotic activity than E. coli with the sfa/foc gene isolated from domestic pigs after 6 hour incubation. In conclusion, screening porcine E. coli for virulence associated genes genes, adhesion to intestinal epithelial cells, and probiotic activity revealed a single important adhesion factor, several probiotic candidates, and showed important differences between E. coli of domestic pigs and wild boars. Y1 - 2013 U6 - https://doi.org/10.1371/journal.pone.0059242 VL - 8 IS - 4 ER - TY - GEN A1 - Guenther, Sebastian A1 - Schierack, Peter A1 - Grobbel, Mirjam A1 - Lübke-Becker, Antina A1 - Wieler, Lothar H. A1 - Ewers, Christa T1 - Real-time PCR assay for the detection of species of the genus Mannheimia. T2 - Journal of Microbiological Methods Y1 - 2008 U6 - https://doi.org/10.1016/j.mimet.2008.05.008 SN - 1872-8359 SN - 0167-7012 VL - 75 IS - 1 SP - 75 EP - 80 ER - TY - GEN A1 - Ali, Aamir A1 - Kolenda, Rafał A1 - Khan, Muhammad Moman A1 - Weinreich, Jörg A1 - Li, Ganwu A1 - Wieler, Lothar H. A1 - Tedin, Karsten A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Novel Avian Pathogenic Escherichia coli Genes Responsible for Adhesion to Chicken and Human Cell Lines T2 - Applied and Environmental Microbiology Y1 - 2020 U6 - https://doi.org/10.1128/AEM.01068-20 SN - 1098-5336 VL - 86 IS - 20 ER - TY - GEN A1 - Bednorz, Carmen A1 - Guenther, Sebastian A1 - Oelschlaeger, Kathrin A1 - Pieper, Robert A1 - Hartmann, Susanne A1 - Tedin, Karsten A1 - Semmler, Torsten A1 - Neumann, Konrad A1 - Schierack, Peter A1 - Bethe, Astrid A1 - Wieler, Lothar H. A1 - Kinnemann, Bianca T1 - Feeding the Probiotic Enterococcus faecium Strain NCIMB 10415 to Piglets Specifically Reduces the Number of Escherichia coli Pathotypes That Adhere to the Gut Mucosa T2 - Applied and Environmental Microbiology Y1 - 2013 U6 - https://doi.org/10.1128/AEM.03138-13 SN - 0099-2240 SN - 1098-5336 VL - 79 IS - 24 SP - 7896 EP - 7904 ER - TY - GEN A1 - Kleta, Sylvia A1 - Nordhoff, M. A1 - Tedin, Karsten A1 - Wieler, Lothar H. A1 - Kolenda, Rafał A1 - Oswald, S. A1 - Oelschlaeger, T. A. A1 - Bleiss, W. A1 - Schierack, Peter T1 - Role of F1C fimbriae, flagella, and secreted bacterial components in the inhibitory effect of probiotic Escherichia coli Nissle 1917 on atypical enteropathogenic E. coli infection. T2 - Infect Immun. Y1 - 2014 U6 - https://doi.org/10.1128/IAI.01431-13 VL - 82 IS - 5 SP - 1801 EP - 1812 ER - TY - GEN A1 - Schierack, Peter A1 - Filter, Matthias A1 - Scharek, Lydia A1 - Toelke, Christiane A1 - Taras, David A1 - Tedin, Karsten A1 - Haverson, Karin A1 - Lübke-Becker, Antina A1 - Wieler, Lothar H. T1 - Effects of Bacillus cereus var. toyoi on immune parameters of pregnant sows T2 - Veterinary Immunology and Immunopathology Y1 - 2009 U6 - https://doi.org/10.1016/j.vetimm.2008.09.002 SN - 0165-2427 VL - 127 IS - 1-2 SP - 26 EP - 37 ER - TY - GEN A1 - Schierack, Peter A1 - Wieler, Lothar H. A1 - Taras, David A1 - Herwig, Volker A1 - Tachu, Babila A1 - Hlinak, Andreas A1 - Schmidt, Michael F. G. A1 - Scharek, Lydia T1 - Bacillus cereus var. toyoi enhanced systemic immune response in piglets. T2 - Veterinary immunology and immunopathology Y1 - 2007 U6 - https://doi.org/10.1016/j.vetimm.2007.03.006 SN - 1873-2534 SN - 0165-2427 VL - 118 IS - 1-2 SP - 1 EP - 11 ER - TY - GEN A1 - Schierack, Peter A1 - Kleta, Sylvia A1 - Tedin, Karsten A1 - Babila, T. B. A1 - Oswald, S. A1 - Oelschlaeger, T. A. A1 - Hiemann, Rico A1 - Paetzold, S. A1 - Wieler, Lothar H. T1 - E. coli Nissle 1917 affects Salmonella adhesion to porcine intestinal epithelial cells T2 - PLoS One Y1 - 2011 U6 - https://doi.org/10.1371/journal.pone.0014712 VL - 6 IS - 2 ER - TY - GEN A1 - Schierack, Peter A1 - Walk, Nicole A1 - Reiter, Katja A1 - Weyrauch, Karl D. A1 - Wieler, Lothar H. T1 - Composition of intestinal Enterobacteriaceae populations of healthy domestic pigs. T2 - Microbiology Y1 - 2007 U6 - https://doi.org/10.1099/mic.0.2007/010173-0 SN - 1465-2080 SN - 1350-0872 VL - 153 IS - 11 SP - 3830 EP - 3837 ER - TY - GEN A1 - Olowe, Olugbenga Adekunle A1 - Choudhary, S. A1 - Schierack, Peter A1 - Wieler, Lothar H. A1 - Makanjuola, O. B. A1 - Olayemi, A. B. A1 - Anjum, M. T1 - Pathotyping bla CTX-M Escherichia coli from Nigeria T2 - European Journal of Microbiology and Immunology N2 - Background: Escherichia coli have become the enterobacteriaceae species most affected by extended-spectrum β-lactamases (ESBLs) in view of the emergence of CTX-M-type ESBLs. These CTX-M-positive E. coli have been reported in numerous regions worldwide. Virulence determinants of already reported CTX-M-positive E. coli were investigated. Methodology: To gain insights into the mechanism underlying this phenomenon, we assessed serogroup, susceptibility pattern and diversity of virulence profiles within a collection of nine blaCTX-M-positive E. coli strains and their virulent determinant using miniaturized DNA microarray techniques. The nine ESBL-positive E. coli isolates were from eight male and one female patient(s) selected for study based on previous work. Virulence potential was inferred by detection of 63 virulence factor (VF) genes. Results: Four (44.4%) of the 9 E. coli isolates exhibited the same set of core characteristics: serotype O8:Hnt, while all were positive for OXA-1, ciprofloxacin resistance. Five of the isolates exhibited highly similar (91% to 100%) VF profiles. Conclusion: The findings describe a broadly disseminated, blaCTX-M-positive and virulent E. coli serogroup with highly homogeneous virulence genotypes, suggesting recent emergence in this zone. Understanding how this clone has emerged and successfully disseminated within the hospital and community, including across national boundaries, should be a public health priority. Y1 - 2013 U6 - https://doi.org/10.1556/EuJMI.3.2013.2.5 SN - 2062-8633 VL - 3 IS - 2 SP - 120 EP - 125 ER - TY - GEN A1 - Schierack, Peter A1 - Walk, Nicole A1 - Ewers, Christa A1 - Wilking, Hendrik A1 - Steinrück, Hartmut A1 - Filter, Matthias A1 - Wieler, Lothar H. T1 - ExPEC-typical virulence-associated genes correlate with successful colonization by intestinal E. coli in a small piglet group. T2 - Environmental Microbiology Y1 - 2008 U6 - https://doi.org/10.1111/j.1462-2920.2008.01595.x SN - 1462-2920 SN - 1462-2912 VL - 10 IS - 7 SP - 1742 EP - 1752 ER - TY - GEN A1 - Bednorz, Carmen A1 - Oelschlaeger, Kathrin A1 - Kinnemann, Bianca A1 - Hartmann, Susanne A1 - Neumann, Konrad A1 - Pieper, Robert A1 - Bethe, Astrid A1 - Semmler, Torsten A1 - Tedin, Karsten A1 - Schierack, Peter A1 - Wieler, Lothar H. A1 - Guenther, Sebastian T1 - The broader context of antibiotic resistance: zinc feed supplementation of piglets increases the proportion of multi-resistant Escherichia coli in vivo T2 - International Journal of Medical Microbiology Y1 - 2013 U6 - https://doi.org/10.1016/j.ijmm.2013.06.004 SN - 1618-0607 SN - 1438-4221 VL - 303 IS - 6-7 SP - 396 EP - 403 ER - TY - GEN A1 - Guenther, Sebastian A1 - Nöckler, Karsten A1 - Nickisch-Rosenegk, Markus von A1 - Landgraf, Maria A1 - Ewers, Christa A1 - Wieler, Lothar H. A1 - Schierack, Peter T1 - Detection of Trichinella spiralis, T. britovi and T. pseudospiralis in muscle tissue with real-time PCR T2 - Journal of Microbiological Methods Y1 - 2008 U6 - https://doi.org/10.1016/j.mimet.2008.06.019 SN - 0167-7012 VL - 75 IS - 2 SP - 287 EP - 292 ER - TY - GEN A1 - Römer, Antje A1 - Wieler, Lothar H. A1 - Schierack, Peter T1 - Analyses of intestinal commensal Escherichia coli strains from wild boars suggest adaptation to conventional pig production conditions T2 - Veterinary Microbiology Y1 - 2012 U6 - https://doi.org/10.1016/j.vetmic.2012.07.009 SN - 0378-1135 VL - 16 IS - 1-2 SP - 122 EP - 129 ER -