TY - CHAP A1 - Kühne, Marco A1 - Wolff, Matthias A1 - Eichner, Matthias A1 - Werner, Steffen A1 - Hoffmann, Rüdiger T1 - Stimmaktivierung eines Spracherkenners unter Berücksichtigung prosodischer Merkmale und der Variabilität der Aussprache T2 - Fortschritte der Akustik, DAGA '05; 31. Deutsche Jahrestagung für Akustik, 14. - 17. März 2005 in München Y1 - 2005 SP - 327 EP - 328 PB - Deutsche Gesellschaft für Akustik CY - Berlin ER - TY - GEN A1 - Werner, Steffen A1 - Eichner, Matthias A1 - Wolff, Matthias A1 - Hoffmann, Rüdiger T1 - Towards spontaneous speech synthesis - Utilizing language model information in TTS T2 - IEEE transactions on speech and audio processing Y1 - 2004 U6 - https://doi.org/10.1109/TSA.2004.828635 SN - 1063-6676 VL - 12 IS - 4 SP - 436 EP - 445 ER - TY - CHAP A1 - Eichner, Matthias A1 - Wolff, Matthias A1 - Hoffmann, Rüdiger A1 - Werner, Steffen ED - Fellbaum, Klaus T1 - Ein kombiniertes Spracherkennungs- / Sprachsynthesesystem auf Phonemebene T2 - Tagungsband zur 11. Konferenz "Elektronische Sprachsignalverarbeitung", 4.-6. September 2000, Cottbus Y1 - 2000 SN - 3-933592-76-3 SP - 141 EP - 149 PB - w.e.b. Universitätsverlag CY - Dresden ER - TY - GEN A1 - Frömmel, Ulrike A1 - Lehmann, Werner A1 - Rödiger, Stefan A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Weinreich, Jörg A1 - Groß, Julia A1 - Roggenbuck, Dirk A1 - Zinke, Olaf A1 - Ansorge, Hermann A1 - Vogel, Steffen A1 - Klemm, Per A1 - Wex, Thomas A1 - Schröder, Christian A1 - Wieler, Lothar H. A1 - Schierack, Peter T1 - Adhesion of human and animal Escherichia coli strains in association with their virulence-associated genes and phylogenetic origins T2 - Applied and environmental microbiology N2 - Intestinal colonization is influenced by the ability of the bacterium to inhabit a niche, which is based on the expression of colonization factors. Escherichia coli carries a broad range of virulence-associated genes (VAGs) which contribute to intestinal (inVAGs) and extraintestinal (exVAGs) infection. Moreover, initial evidence indicates that inVAGs and exVAGs support intestinal colonization. We developed new screening tools to genotypically and phenotypically characterize E. coli isolates originating in humans, domestic pigs, and 17 wild mammal and avian species. We analyzed 317 isolates for the occurrence of 44 VAGs using a novel multiplex PCR microbead assay (MPMA) and for adhesion to four epithelial cell lines using a new adhesion assay. We correlated data for the definition of new adhesion genes. inVAGs were identified only sporadically, particularly in roe deer (Capreolus capreolus) and the European hedgehog ( Erinaceus europaeus). The prevalence of exVAGs depended on isolation from a specific host. Human uropathogenic E. coli isolates carried exVAGs with the highest prevalence, followed by badger (Meles meles) and roe deer isolates. Adhesion was found to be very diverse. Adhesion was specific to cells, host, and tissue, though it was also unspecific. Occurrence of the following VAGs was associated with a higher rate of adhesion to one or more cell lines: afa-dra, daaD, tsh, vat, ibeA, fyuA, mat, sfa-foc, malX, pic, irp2, and papC. In summary, we established new screening methods which enabled us to characterize large numbers of E. coli isolates. We defined reservoirs for potential pathogenic E. coli. We also identified a very broad range of colonization strategies and defined potential new adhesion genes. Y1 - 2013 U6 - https://doi.org/10.1128/AEM.01384-13 SN - 1098-5336 SN - 0099-2240 VL - 79 IS - 19 SP - 5814 EP - 5829 ER - TY - GEN A1 - Fahrion, Marc-Steffen A1 - Draeger, Susan A1 - Sinn, Michel A1 - Altinisik, Leman A1 - Sobek, Werner T1 - AKTIVkrankenhaus – Modernisierungsstrategie für ein klimaneutrales Krankenhausareal T2 - Bauphysik Y1 - 2018 SN - 0171-5445 SN - 1437-0980 VL - 40 IS - 4 SP - 169 EP - 179 ER -