TY - CHAP A1 - Cueva, M. A1 - Wolff, Matthias A1 - Werner, Steffen A1 - Eichner, Matthias A1 - Hoffmann, Rüdiger T1 - Integration von Finite State Transducers in das UASR-System T2 - ITG-Fachtagung Sprachkommunikation, Kiel, 26. - 28. 4. 2006 Y1 - 2006 SN - 978-3-8007-2955-5 PB - VDE-Verlag CY - Berlin [u.a.] ER - TY - CHAP A1 - Eichner, Matthias A1 - Kühne, M. A1 - Werner, Steffen A1 - Wolff, Matthias ED - Kroschel, Kristian T1 - Sprachtechnologien in der Lernumgebung eines internet-basierten Studienganges T2 - Tagungsband zur 14. Konferenz "Elektronische Sprachsignalverarbeitung", Karlsruhe, 24.-26.09.2003 Y1 - 2003 SN - 3-935712-83-9 SP - 370 EP - 377 PB - w.e.b. Universitätsverlag CY - Dresden ER - TY - GEN A1 - Fassold, Alexander A1 - Falk, Werner A1 - Anders, Sven A1 - Hirsch, Thomas A1 - Mirsky, Vladimir M. A1 - Straub, Rainer H. T1 - Soluble neuropilin-2, a nerve repellent receptor, is increased in rheumatoid arthritis synovium and aggravates sympathetic fiber repulsion and arthritis T2 - Arthritis and Rheumatology N2 - In inflammatory lesions, sympathetic nerve fibers disappear soon after the start of inflammation. We identified sympathetic nerve repellents as possible causal agents in rheumatoid arthritis (RA). On nerve terminals, repellent factors bind to neuropilin-2 and its coreceptor. The aim of this study was to investigate the role of neuropilin-2 in the synovial tissue of patients with RA and patients with osteoarthritis (OA) and in experimental arthritis. The density of neuropilin-2–positive fibers and cells positive for semaphorin 3F (a sympathetic repellent) was investigated using immunofluorescence staining. Enzyme-linked immunosorbent assay was used to detect soluble neuropilin-2 in body fluids from patients with RA and patients with OA. An axon outgrowth assay and a neuropilin-2 Fc fusion construct (neuropilin-2Fc) were used to investigate semaphorin 3F–induced sympathetic nerve repulsion. In an animal model of type II collagen–induced arthritis, soluble neuropilin-2Fc was studied in vivo. The synovial density of neuropilin-2–positive sympathetic nerve fibers was lower in RA than in OA, but the density of cells positive for semaphorin 3F was similar. In synovial fluid, the level of soluble neuropilin-2 was markedly higher in RA compared with OA. Mouse sympathetic ganglia served as an excellent model with which to study semaphorin 3F–induced nerve fiber repulsion. Neuropilin-2 and its coreceptor were present on sympathetic neurons, and semaphorin 3F bound to neuropilin-2Fc (binding constant 96 nmoles/liter). Semaphorin 3F dose-dependently increased sympathetic nerve fiber repulsion (at a 50% maximum response concentration of 160–210 nmoles/liter). In contrast to our expectations, soluble neuropilin-2Fc did not inhibit repulsion but increased the repellent effect of semaphorin 3F. In experimental arthritis, therapy with neuropilin-2Fc aggravated arthritis. Soluble neuropilin-2 has no antirepellent activity but aggravates sympathetic nerve fiber repulsion and arthritis. Increased shedding of neuropilin-2 is probably an unfavorable sign in RA. Y1 - 2009 U6 - https://doi.org/10.1002/art.24860 SN - 2326-5205 VL - 60 IS - 10 SP - 2892 EP - 2901 ER - TY - GEN A1 - Wrobel, Nadia A1 - Deininger, Werner A1 - Hegemann, Peter A1 - Mirsky, Vladimir M. T1 - Covalent immobilization of oligonucleotides on electrodes T2 - Colloids and Surfaces B: Biointerfaces N2 - 32P-labeled DNA oligomers were immobilized onto self-assembled monolayers formed by carboxy-modified alkylthiols on gold electrodes. The optimal conditions were evaluated by comparing of surface density of oligonucleotides measured for different immobilization conditions before and after desorption of physically adsorbed nucleotides. The results demonstrate that the physical adsorption depends strongly on immobilization conditions being typically 10 times higher than the chemical immobilization. Under optimal immobilization conditions, the surface density of oligonucleotides was more than 20% of the value calculated for a densely packed monolayer. KW - DNA KW - DNA immobilization KW - DNA sensor KW - Biosensor KW - Hybridization Y1 - 2003 U6 - https://doi.org/10.1016/S0927-7765(03)00155-3 SN - 0927-7765 VL - 32 IS - 2 SP - 157 EP - 162 ER - TY - GEN A1 - Haase, R. A1 - Werner, M. A1 - Kräusel, V. A1 - Alimov, Artem A1 - Sviridov, Alexander A1 - Härtel, Sebastian T1 - Improved formability of HMGF components by preforming in an upset bulging process T2 - IOP Conference Series Y1 - 2022 U6 - https://doi.org/10.1088/1757-899X/1238/1/012018 N1 - Materials Science and Engineering, IDDRG 2022; 06.06.-10.06.2022, Lorient, France VL - 1238 PB - IOP Publishing Ltd. CY - Bristol ER - TY - GEN A1 - Nast, A. A1 - Mrowietz, U. A1 - Kragballe, K. A1 - Jong, E. M. G. J. de A1 - Puig, L. A1 - Reich, K. A1 - Warren, R. B. A1 - Werner, R. A1 - Kopkow, Christian A1 - Schmitt, Jochen T1 - Barriers to the prescription of systemic therapies for moderate-to-severe psoriasis T2 - Archives of Dermatological Research Y1 - 2013 U6 - https://doi.org/10.1007/s00403-013-1372-3 SN - 1432-069X SN - 0340-3696 VL - 305 IS - 10 SP - 899 EP - 907 ER - TY - GEN A1 - Raab, Thomas A1 - Krümmelbein, Julia A1 - Schneider, Anna A1 - Gerwin, Werner A1 - Maurer, Thomas A1 - Naeth, M. Anne T1 - Initial Ecosystem Processes as Key Factors of Landscape Development - A Review T2 - Physical Geography Y1 - 2012 SN - 1930-0557 VL - 33 IS - 4 SP - 305 EP - 343 ER - TY - GEN A1 - Schaaf, Wolfgang A1 - Bens, Oliver A1 - Fischer, Anton A1 - Gerke, Horst H. A1 - Gerwin, Werner A1 - Grünewald, Uwe A1 - Holländer, Hartmut M. A1 - Kögel-Knabner, Ingrid A1 - Mutz, Michael A1 - Schloter, Michael A1 - Schulin, Rainer A1 - Veste, Maik A1 - Winter, Susanne A1 - Hüttl, Reinhard F. T1 - Patterns and processes of initial terrestrial-ecosystem development T2 - Journal of Plant Nutrition and Soil Science N2 - Ecosystems are characterized as complex systems with abiotic and biotic processes interacting between the various components that have evolved over long-term periods. Most ecosystem studies so far have been carried out in mature systems. Only limited knowledge exists on the very initial phase of ecosystem development. Concepts on the development of ecosystems are often based on assumptions and extrapolations with respect to structure–process interactions in the initial stage. To characterize the effect of this initial phase on structure and functioning of ecosystems in later stages, it is necessary to disentangle the close interaction of spatial and temporal patterns of ecosystem structural assemblages with processes of ecosystem development. The study of initial, less complex systems could help to better identify and characterize coupled patterns and processes. This paper gives an overview of concepts for the initial development of different ecosystem compartments and identifies open questions and research gaps. The artificial catchment site “Chicken Creek” is introduced as a new research approach to investigate these patterns and processes of initial ecosystem development under defined boundary conditions. This approach allows to integrate the relevant processes with related pattern and structure development over temporal and spatial scales and to derive thresholds and stages in state and functioning of ecosystems at the catchment level. KW - soils KW - pedogenesis KW - vegetation succession KW - microbial succession KW - artificial catchment Y1 - 2011 U6 - https://doi.org/10.1002/jpln.201000158 SN - 1522-2624 VL - 174 IS - 2 SP - 229 EP - 239 ER - TY - CHAP A1 - Hinz, Christoph A1 - Gerwin, Werner A1 - Schaaf, Wolfgang A1 - Elmer, Michael A1 - Flühler, Hannes A1 - Holländer, Hartmut M. A1 - Hüttl, Reinhard F. T1 - State transitions in the artificial catchment Huehnerwasser (Chicken Creek): Ecosystems development 8 years after initialization : abstract T2 - Soil-mediated Drivers of Coupled Biogeochemical and Hydrological Processes across Scales, AGU Chapman Conference Biosphere II, Tucson, Arizona, USA, 21-24 October 2013 Y1 - 2013 UR - http://chapman.agu.org/soil-mediated/files/2013/10/AGU-CC13SD-3.pdf SP - S. 34 ER - TY - GEN A1 - Veste, Maik A1 - Dominik, R. A1 - Dimitrov, M. A1 - Fischer, Annette A1 - Gerwin, Werner A1 - Schaaf, Wolfgang ED - Stadler, Jutta ED - Schöppe, Felix ED - Frenzel, Mark T1 - Novel drone-based system for ecosystem monitoring - application, analysis and interpretation T2 - EURECO-GFOE 2008, Proceedings Y1 - 2008 SN - 978-3-00-025522-9 SP - S. 809 PB - Gesellschaft für Ökologie CY - Berlin ER -