TY - GEN A1 - Wanner, Manfred A1 - Birkhofer, Klaus A1 - Puppe, Daniel A1 - Shimano, Satoshi D. A1 - Shimizu, Miki T1 - Tolerance of testate amoeba species to rising sea levels under laboratory conditions and in the South Pacific T2 - Pedobiologia Y1 - 2020 SN - 0031-4056 VL - 79 ER - TY - GEN A1 - Saito, Ryota A1 - Koizumi, Ryota A1 - Sakai, Tatsuya A1 - Shimizu, Taiga A1 - Ono, Taiki A1 - Wanner, Manfred A1 - Takyu, Toshio A1 - Tanaka, Toshihiko A1 - Sogame, Yoichiro T1 - Recovery of proliferative capability in gamma irradiated Colpoda cucullus (ciliated protist) resting cysts and its radiation hormesis T2 - Protistology N2 - The resting cyst is one of the quietest life stages (i.e. cryptobiosis) with hardly measurable or even ceased metabolic activity. However, are the resting cysts just resting? Here, to our knowledge, we report for the first time radiation hormesis in resting cysts. In the present study, we focused on the proliferative capability of excysted cells from 500–4000 Gy-gamma-irradiated Colpoda cucullus resting cysts by a bioassay method. Generally, excysted cells from irradiated cysts lose their proliferative capability. However, we observed an increased proliferative capability promoted by low doses of irradiation. Cells, which had excysted from 500-Gy-irradiated cysts, restored their proliferation capability with a recovery period of 12 h and 24 h before excysting. Additionally, cells, which had excysted from 500-4000-Gy irradiated cysts, recovered proliferative capability by re-forming resting cysts. Here, the proliferative capability of excysted cells from 500-Gy-irradiated cysts was even higher than that of non-irradiated. KW - cyst KW - cell proliferation KW - hormesis KW - stress repair KW - recover Y1 - 2020 U6 - https://doi.org/10.4467/16890027AP.19.006.10837 SN - 1680-0826 VL - 14 IS - 3 SP - 160 EP - 171 ER - TY - GEN A1 - Aoki, Yoshiyuki A1 - Sogame, Yoichiro A1 - Wanner, Manfred A1 - Mazei, Yuri A1 - Shimano, Satoshi D. T1 - A new testate amoeba, Matsakision ogawaraensis sp. nov. (Silicofilosea: Incertae sedis Euglyphida) from lake shore sand of northern Japan T2 - Acta Protozoologica N2 - A new species of a testate amoeba, Matsakision ogawaraensis sp. nov., is described from Ogawara pond, Aomori Prefecture, Japan. This is the third species in the genus Matsakision. Matsakision ogawaraensis sp. nov. is distinguished from two other species by the specific form of the shell in apertural view, which is triangular (the shells of other species are circular or elliptic). In addition, it has a chitinous lip around the aperture. For the first time, long needle-like pseudopodia have been observed. KW - testate amoeba KW - Matsakision KW - Rhizaria KW - lake KW - Japan Y1 - 2020 UR - https://www.ejournals.eu/pliki/art/17797/pl U6 - https://doi.org/10.4467/16890027AP.20.008.12676 SN - 1689-0027 SN - 0065-1583 VL - 59 IS - 2 SP - 99 EP - 105 ER - TY - GEN A1 - Wanner, Manfred A1 - Sogame, Yoichiro A1 - Shimizu, Miki T1 - Testate amoebae from two low-lying tropical islets of Tuvalu, South Pacific T2 - Pedobiologia : journal of soil ecology N2 - Testate amoebae are a polyphyletic group of ubiquitous unicellular eukaryotic micro-organisms. They occur in high abundances in freshwater and terrestrial habitats, where they play an important role in biogeochemical and ecological processes. Terrestrial amoeba data from small tropical islands are rarely available despite the fact that they are interesting for investigations on global change induced sea level rise, biodiversity and biogeography. Based on a previously published paper on the impact of increased salinity to testate amoebae, the aim of this study is to present data on amoebal biodiversity from two remote, low-lying tropical islets of Tuvalu (South Pacific). In total 47 species and subspecific taxa of testate amoebae were recorded. These findings are based on 21 soil samples from 7 terrestrial habitats examined microscopically. Most species found were relatively small (< 100 μm) and considered as cosmopolitan. Non-metric multidimensional scaling ordination (NMDS) separated the samples by habitat structure and flooding regime rather than islet origin. Despite the apparently remote character of the study sites, human influence on distribution and biogeography of testate amoebae cannot be excluded. KW - Soil protists KW - Community structure KW - Sea level rise KW - Remote Pacific islands Y1 - 2021 U6 - https://doi.org/10.1016/j.pedobi.2021.150732 SN - 0031-4056 SN - 1873-1511 SP - 87 EP - 88 ER - TY - GEN A1 - Wanner, Manfred A1 - Sogame, Yoichiro A1 - Shimizu, Miki T1 - An elevation transect study of testate amoeba communities up to 4000 m a.s.l. on Mount Kinabalu, Borneo T2 - European Journal of Protistology N2 - Testate amoebae are a polyphyletic assemblage of unicellular eukaryotes with a specific shell. They occur frequently in soil and freshwater habitats all over the world, important for ecosystem functioning and useful for bio-indication. Terrestrial amoeba data from Borneo or Malaysia are rarely available. In this study, data on species composition and relative abundance were presented along a transect from 1730 m up to 4000 m above sea level from 10 elevational levels on Mt. Kinabalu, Malaysian Borneo. In total 78 morphotypes of testate amoebae were recorded. Most were small (under 100 µm) and supposedly cosmopolitan. However, Certesella certesi with limited geographical distribution and some rare taxa (e.g., Padaungiella lageniformis cordiformis, Placocista jurassica, Trinema chardezi) and two undescribed morphospecies were also observed. Presence/absence and relative abundance data of testate amoebae were associated to elevation with a pronounced change at the tree line, as revealed by non-metric multidimensional scaling ordination analysis. Our data from Southeast Asia contribute to the actual discussion on distributional patterns considering elevation gradients and biogeography of eukaryotic microbes. KW - Soil protists KW - Community structure KW - Malaysia Y1 - 2022 U6 - https://doi.org/10.1016/j.ejop.2022.125868 SN - 0932-4739 VL - Vol. 83 ER - TY - GEN A1 - Tsyganov, Andrey N. A1 - Bobrov, Anatoly A. A1 - Shimano, Satoshi D. A1 - Mitchell, Edward A. D. A1 - Hagiwara, Yasuo A1 - Wall, Adeline A. J. A1 - Wanner, Manfred T1 - Distribution of soil testate amoeba assemblages along an elevation gradient on Mount Fuji T2 - European Journal of Protistology N2 - Elevation gradients have been useful to study distributional patterns of soil organisms since the time of Humboldt but only recently these patterns have been studied for soil microorganisms. Here we report the results on species diversity and composition of soil- and moss-dwelling testate amoeba assemblages along a 1400 m elevation gradient (904–2377 m a.s.l.) on Mount Fuji (Japan) from temperate forest to alpine vegetation. In total, 95 testate amoeba taxa belonging to 29 genera were identified. The core of testate amoeba assemblages was formed by ubiquitous species such as Trinema lineare, Euglypha laevis, Cryptodifflugia oviformis, and Trinema complanatum. However, several taxa with limited geographic distribution were also observed (e.g., Centropyxis latideflandriana, C. stenodeflandriana, Plagiopyxis cf. barrosi, Heleopera rectangularis, and Distomatopyxis couillardi). Species diversity indices (species richness and Shannon’s index) were characterised by bell-shaped patterns peaking at ∼ 1700 m in the subalpine mixed conifer-deciduous forest. The species composition of testate amoeba assemblages was best explained by the vegetation types which accounted for 12.3% of the total variation. Overall, these findings indicate that elevation effects on species composition of testate amoeba assemblages are strongly mediated by vegetation. KW - Protists KW - Biogeography KW - Elevation KW - Vegetation type KW - Distribution Y1 - 2022 U6 - https://doi.org/10.1016/j.ejop.2022.125894 SN - 0932-4739 VL - 84 SP - 1 EP - 12 ER - TY - GEN A1 - Wanner, Manfred A1 - Siemensma, Ferry A1 - Prasad Acharja, Indra A1 - Tshering, Jigme A1 - Khandu, Pema A1 - Lal Gajmer, Santa A1 - Gyeltshen, Choki A1 - Dorji, Tshering A1 - Tenzin, Kinley A1 - Shimano, Satoshi D. T1 - Testate amoebae from the wetlands of the Phobjikha Valley of Bhutan, the Eastern Himalayas T2 - European Journal of Protistology N2 - The Kingdom of Bhutan in the Eastern Himalayas harbors a rich biodiversity. However, its detailed documentation still needs to be created, including not only macroorganisms but also protists. In the present study, as many as 105 taxa of testate amoebae were identified and directly counted in soil suspension samples collected from the wetlands of the Gangtey-Phobji Valley (generally Phobjikha), Wangdue Phodrang district. The most dominant were cosmopolitan species, e.g., Euglypha rotunda, Trinema lineare, T. complanatum, T. enchelys, Phryganella acropodia, and Plagiopyxis declivis. However, some taxa such as Centropyxis deflandriana, C. stenodeflandriana, Hoogenraadia cf. humicola, Pareuglypha reticulata, and Sphenoderia chardezi have a geographically restricted distribution and/or are considered rare. Some specimens could not be assigned to already described species (Centropyxis dentata sp. nov. and Difflugia fusiforma sp. nov.) or were highly similar to known species but differed in size or other taxonomically important characteristics (e.g., Hoogenraadia cf. humicola and Planhoogenraadia sp.). To our knowledge, this study is the first to present a comprehensive list of testate amoebae from Bhutan, including some new and rare species. The discovery of a remarkable diversity of testate amoebae in only a few soil samples suggests that this remote and largely unexplored region likely harbors many more intriguing species. KW - Testate amoebae KW - Soil habitats KW - Biodiversity KW - Taxonomy KW - New species Y1 - 2024 U6 - https://doi.org/10.1016/j.ejop.2024.126125 SN - 0932-4739 IS - 96 ER - TY - GEN A1 - Saito, Ryota A1 - Yamanobe, Hiroki A1 - Yabuki, Kazuma A1 - Suzuki, Tomohiro A1 - Saito, Takeru A1 - Hakozaki, Shuntaro A1 - Wanner, Manfred T1 - Salinity tolerance in resting cysts of colpodid ciliates : comparative transcriptomics analysis and chemical analysis of cyst walls to investigate their tolerance capability. T2 - Current research in microbial sciences N2 - The formation of resting cysts is a strategy for survival in unfavorable environments by single cell organisms such as protists. Here, we show that Colpoda resting cysts have high salinity tolerance and investigate the changes in gene expression that underpin this effect. Colpoda resting cysts can tolerate saline conditions up to 3.5 % NaCl. A comparative transcriptome analysis of vegetative cells and resting cysts showed that the relative levels of expression of genes associated with membrane function increased in resting cysts. These changes in gene expression suggest that reconstruction of the plasma membrane is associated with salinity tolerance. The resting cyst forms cyst-specific cellular structure known as the cyst wall. The outer shell-like layer, called the ectocyst, while the inner multiple layers, known as the endocyst. The chemical analysis showed ectocyst contains chitin and endocyst contains several proteins. These structures can protect cells by acting as a biological armor or protective materials. The results of this study offer a possible scenario in which salinity tolerance enables the widespread dispersal of protists. KW - Protist KW - Chitin KW - Biodiversity KW - Actin KW - Cryptobiosis KW - NGS KW - NGS Dormancy Y1 - 2025 U6 - https://doi.org/10.1016/j.crmicr.2025.100371 SN - 2666-5174 VL - 8 SP - 1 EP - 15 PB - Elsevier CY - Amsterdam ER - TY - GEN A1 - Wanner, Manfred A1 - Elmer, Michael A1 - Sommer, Michael A1 - Funk, Roger A1 - Puppe, Daniel T1 - Testate amoebae colonizing a newly exposed land surface are of airborne origin T2 - Ecological Indicators N2 - We hypothesized that at the very beginning of terrestrial ecosystem development, airborne testate amoebae play a pivotal role in facilitating organismic colonization and related soil processes. We, therefore, analyzed size and quantity of airborne testate amoebae and immigration and colonization success of airborne testate amoebae on a new land surface (experimental site “Chicken Creek”, artificial post-mining water catchment). Within an altogether 91-day exposure of 70 adhesive traps, 12 species of testate amoebae were identified to be of airborne origin. Phryganella acropodia (51% of all individuals found, diameter about 35–45 μm) and Centropyxis sphagnicola (23% of all individuals found, longest axis about 55–68 μm), occurred most frequently in the adhesive traps. We extrapolated an aerial amoeba deposition of 61 individuals d−1 m−2 (living and dead individuals combined). Although it would be necessary to have a longer sequence (some additional years), our analysis of the “target substrate” of aerial immigration (catchment site) may point to a shift from a stochastic (variable) beginning of community assembly to a more deterministic (stable) course. This shift was assigned to an age of seven years of initial soil development. Although experienced specialists are necessary to conduct these time-consuming studies, the presented data suggest that terrestrial amoebae are suitable indicators for initial ecosystem development and utilization. Y1 - 2015 U6 - https://doi.org/10.1016/j.ecolind.2014.07.037 SN - 1470-160x VL - 48 SP - 55 EP - 62 ER - TY - CHAP A1 - Wöllecke, Jens A1 - Elmer, Michael A1 - Anders, Kenneth A1 - Durka, Walter A1 - Wanner, Manfred ED - Wöllecke, Jens ED - Anders, Kenneth ED - Durka, Walter ED - Elmer, Michael ED - Wanner, Manfred ED - Wiegleb, Gerhard T1 - Landschaft im Wandel - natürliche und anthropogene Besiedlung der Niederlausitzer Bergbaufolgelandschaft - eine Einführung T2 - Landschaft im Wandel - natürliche und anthropogene Besiedlung der Niederlausitzer Bergbaufolgelandschaft Y1 - 2007 SN - 978-3-8322-6336-2 SP - 1 EP - 4 PB - Shaker CY - Aachen ER - TY - GEN A1 - Elmer, Michael A1 - Rißmann, Cornelia A1 - Wanner, Manfred A1 - Hohberg, Karin A1 - Xylander, Willi E. R. T1 - Die Bodenfauna in den Wäldern der Schlabendorfer Felder - natürliche Besiedlung und Wechselwirkungen mit der forstlichen Bewirtschaftung T2 - Biologische Studien Y1 - 2010 SN - 1432-4199 IS - 39 SP - 36 EP - 47 ER - TY - CHAP A1 - Wanner, Manfred A1 - Elmer, Michael A1 - Bahrt, Alexander A1 - Balkenhol, Birgit A1 - Düker, Christian A1 - Hohberg, Karin A1 - Nährig, Dietrich A1 - Hüttl, Reinhard F. A1 - Xylander, Willi E. R. ED - Bröring, Udo ED - Wanner, Manfred T1 - Entwicklung der Biodiversität junger Böden der Niederlausitzer Bergbaufolgelandschaft T2 - Entwicklung der Biodiversität in der Bergbaufolgelandschaft im Gefüge von Ökologie und Sozioökonomie Y1 - 2007 SP - 75 EP - 78 PB - BTU Cottbus CY - Cottbus ER -