TY - GEN A1 - Cherny, Vladimir V. A1 - Sihuralidze, M. G. A1 - Mirsky, Vladimir M. A1 - Sokolov, Valeri S. T1 - An effect of the phospholipase A2 on the electrical potential distribution through lipid bilayer T2 - Biological membranes Y1 - 1992 VL - 7 SP - 733 EP - 740 ER - TY - GEN A1 - Cherny, Vladimir V. A1 - Donath, E. A1 - Mirsky, Vladimir M. A1 - Paulitchke, M. A1 - Sokolov, Valeri S. T1 - Modification of lipid bilayer properties due to sodium hypochlorite. I. Effect on red blood cell membranes T2 - Biological membranes Y1 - 1992 VL - 9 IS - 1 SP - 60 EP - 65 ER - TY - PAT A1 - Mirsky, Vladimir M. A1 - Mirsky, Alex T1 - Distributed reference spots in chemical sensing Y1 - 2006 ER - TY - GEN A1 - Lyutov, Vladimir V. A1 - Ivanov, Svetlozar D. A1 - Mirsky, Vladimir M. A1 - Tsakova, Vessela T. T1 - Polyaniline doped with poly(acrylamidomethylpropanesulphonic acid): electrochemical behaviour and conductive properties in neutral solutions T2 - Chemical Papers N2 - Poly(2-acrylamido-2-methyl-1-propanesulphonic acid) (PAMPSA)-doped polyaniline (PANI) layers are synthesised in the presence of sulphuric and perchloric acids. The effects of the inorganic acid as well as of the electrochemical synthetic procedure (potentiostatic and potentiodynamic deposition) and thickness of the polymer layers are studied. The focus is directed towards the pH dependence of the electrochemical redox activity and conductivity of the PAMPSA-doped PANI layers obtained under different conditions. Ascorbic acid oxidation is used as a test reaction to study the electrocatalytic behaviour of various PAMPSA-doped PANI layers in neutral solution. It is found that the type of inorganic component present in the polymerisation solution has a marked effect on the extent of doping in acidic solutions as well as on the redox electroactivity in neutral solutions. A comparison between potentiostatically and potentiodynamically synthesised layers at pH 7 shows a markedly lower conductance and lower extent of redox charge preservation in the case of potentiodynamic synthesis. The PANI electrocatalytic activity for ascorbic acid oxidation is also dependent on the polymer electrodeposition procedure, with potentiostatically synthesised layers exhibiting better electrocatalytic performance. KW - polyaniline KW - polyacids KW - PAMPSA KW - conductance KW - electrochemical Y1 - 2013 U6 - https://doi.org/10.2478/s11696-013-0341-9 SN - 1336-9075 VL - 67 IS - 8 SP - 1002 EP - 1011 ER - TY - GEN A1 - Tsakova, Vessela T. A1 - Ivanov, Svetlozar D. A1 - Lange, Ulrich A1 - Stoyanova, Aneliya A1 - Lyutov, Vladimir V. A1 - Mirsky, Vladimir M. T1 - Electroanalytical applications of nanocomposites from conducting polymers and metallic nanoparticles prepared by layer-by-layer deposition T2 - Pure and Applied Chemistry N2 - Layer-by-layer (LbL) deposition is a convenient technique for the formation of ultra-thin nanocomposite layers containing metallic nanoparticles (NPs) and conducting polymers (CPs). The advantages of this approach for producing composite layers suitable for electroanalytical applications are discussed. Examples of electroanalytical applications of LbL-deposited composites are presented. Composite layers consisting of polyaniline (PANI) and Pd NPs are used for hydrazine oxidation. The PANI–Au NPs system is applied for dopamine (DA) and uric acid (UA) oxidation. KW - conducting polymers KW - dopamine KW - electrochemistry KW - hydrazine KW - layer-by-layer deposition KW - nanoparticles KW - polyaniline (PANI) KW - uric acid Y1 - 2010 U6 - https://doi.org/10.1351/PAC-CON-10-08-01 SN - 1365-3075 VL - 83 IS - 2 SP - 345 EP - 358 ER - TY - GEN A1 - Broncová, Gabriela A1 - Anikin, Sergey A1 - Lange, Ulrich A1 - Matějka, Pavel A1 - Krondak, Martin A1 - Král, Vladimír A1 - Mirsky, Vladimir M. T1 - Electrochemical and spectroscopic properties of poly-4,4′-dialkoxy-2,2′-bipyrroles T2 - Journal of Solid State Electrochemistry N2 - The structure and the electrochemical and spectral properties of two conductive electrochemically polymerized substituted bipyrroles 4,4′-methoxy-2,2′-bipyrrole and 4,4′-buthoxy-2,2′-bipyrrole were studied and compared. The polymers were characterized by cyclic voltammetry, FT-Raman spectroscopy, scanning electron microscopy, and in situ conductivity measurements at different pH and redox state. KW - Conducting polymer KW - Polydialkoxybipyrroles KW - Raman spectroscopy KW - Cyclic voltammetry KW - Conductivity Y1 - 2010 U6 - https://doi.org/10.1007/s10008-009-0911-5 SN - 1432-8488 SN - 1433-0768 VL - 14 IS - 6 SP - 1035 EP - 1044 ER - TY - GEN A1 - Lange, Ulrich A1 - Ivanov, Svetlozar D. A1 - Lyutov, Vladimir V. A1 - Tsakova, Vessela T. A1 - Mirsky, Vladimir M. T1 - Voltammetric and conductometric behaviour of self assembled multilayer nanocomposites of polyaniline and gold nanoparticles T2 - Journal of Solid State Electrochemistry N2 - Multilayer nanocomposites from polyaniline (PANI) and gold nanoparticles (AuNPs) were formed by layer-by-layer deposition. The formation of PANI–AuNPs multilayer structures was monitored by UV-vis absorption spectroscopy and cyclic voltammetry. Each deposited bilayer of PANI–AuNPs led to a monotonous and almost linear increase in both optical absorbance and the first current peak of PANI oxidation. The prepared multilayer nanocomposites were characterized by in situ conductivity measurements at different pH and potential and by transmission electron microscopy. Finally, chemosensitive properties of the new material based on the intrinsic affinity of gold nanoparticles were studied. Changes in the film resistance on exposure to vapors of mercury and sulfur-containing compounds were observed. KW - Polyaniline KW - Gold nanoparticles KW - Layer-by-layer deposition KW - Chemoresistor KW - In situ conductivity measurements KW - Nanocomposite Y1 - 2010 U6 - https://doi.org/10.1007/s10008-009-0922-2 SN - 1432-8488 SN - 1433-0768 VL - 14 IS - 7 SP - 1261 EP - 1268 ER - TY - GEN A1 - Cígler, Petr A1 - Král, Vladimír A1 - Kožíšek, Milan A1 - Konvalinka, Jan A1 - Mirsky, Vladimir M. T1 - Anomalous adsorptive properties of HIV protease: Indication of two-dimensional crystallization? T2 - Colloids and Surfaces B: Biointerfaces N2 - Adsorption of HIV protease onto surfaces that are usually considered to be protein-resistant was studied quantitatively using surface plasmon resonance. Adsorption onto gold surfaces functionalized by OH-terminated alkyl chains was much stronger than onto oligo(ethylene glycol)-terminated surfaces. Equilibrium and kinetic adsorption constants were determined. An anomalous mutual attraction between adsorbate molecules was observed, indicating the possibility of two-dimensional crystallization of HIV protease. These results are applicable for the design of sensors/biosensors for HIV protease resistance detection and for proper manipulation of this enzyme in laboratory devices. KW - HIV protease KW - Protein adsorption KW - Protein-resistant surfaces KW - Self-assembled monolayer KW - Surface plasmon resonance (SPR) Y1 - 2008 U6 - https://doi.org/10.1016/j.colsurfb.2008.01.011 SN - 0927-7765 VL - 64 IS - 1 SP - 145 EP - 149 ER - TY - GEN A1 - Riepl, Michael A1 - Mirsky, Vladimir M. A1 - Novotny, Ivan A1 - Tvarozek, Vladimir A1 - Rehacek, Vlastimil A1 - Wolfbeis, Otto S. T1 - Optimization of capacitive affinity sensors: drift suppression and signal amplification T2 - Analytica Chimica Acta N2 - The detection limit of capacitive affinity sensors based on the gold–alkanethiol system can be improved by optimization of sensor preparation and by signal amplification. The dissociation of the gold–sulfur binding is often a critical point leading to operative errors of such sensors. The stability of self-assembled monolayers prepared with different thiols on gold electrodes in aqueous and organic solvents was studied by the capacitive technique. The results show that monolayers made of 16-mercaptohexadecanoic acid are stable in aqueous solution and can be hardly extracted from a gold surface by ethanol, methanol, or dioxane, while a considerable damage of self-assembled monolayers was observed due to incubation in chloroform or dimethylformamide. In contrast, self-assembled monolayers made from short-chain disulfides or thiols (such as 3,3′-dithio-bis(propionic acid N-hydroxysuccinimide ester) or 11-mercaptoundecanoic acid) displayed a poor stability in aqueous phase. Capacitive affinity sensors based on these short-chain thiols showed a considerable drift of the signal. The use of long-chain thiols resulted in a stable signal; it was applied to compare capacitive effects due to immobilization of different biological molecules and for preparation of different biosensors. The response of capacitive biosensors can be amplified by formation of a sandwich structure. This principle was illustrated by subsequent adsorption of polyclonal anti-HSA after binding of HSA with a sensor for HSA based on monoclonal antibodies. KW - Affinity sensor KW - Self-assembly KW - Capacitive sensor KW - Signal amplification KW - Immunosensor KW - Biosensor Y1 - 1999 U6 - https://doi.org/10.1016/S0003-2670(99)00195-6 SN - 0003-2670 VL - 392 IS - 1 SP - 77 EP - 84 ER - TY - GEN A1 - Rehacek, Vlastimil A1 - Novotny, Ivan A1 - Tvarozek, Vladimir A1 - Riepl, Michael A1 - Hirsch, T. A1 - Mass, Markus A1 - Schweiss, R. A1 - Mirsky, Vladimir M. A1 - Wolfbeis, Otto S. ED - Breza, Jura ED - Donoval, Daniel T1 - Capacitive detection in thin film chemical sensors and biosensors T2 - Advanced Semiconductor Devices and Microsystems N2 - Thin film electrode chips on silicon substrate have been realized and utilized in development of different chemo- and biosensors. These affinity sensors are based on capacitive transducing and their general structure is thin film Au/alkanethiol/receptor. Several applications of thin film gold electrodes in capacitive sensors are presented. Y1 - 1998 SN - 0-7803-4909-1 SP - 255 EP - 258 PB - IEEE CY - Piscataway, NJ ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Riepl, Michael A1 - Krause, Christian A1 - Novotny, Ivan A1 - Splonskowski, Markus A1 - Rehacek, Vlastimil A1 - Tvarozek, Vladimir A1 - Hummel, Helmut A1 - Wolfbeis, Otto S. T1 - Thin film electrodes for capacitive chemo- and biosensors: an optimization of the electrodes geometry T2 - Materials Science Forum KW - Biosensor KW - Capacitance KW - Chemical Sensors KW - Electrode Y1 - 1998 U6 - https://doi.org/10.4028/www.scientific.net/MSF.287-288.423 SN - 1662-9752 VL - 287-288 SP - 423 EP - 426 ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Vasjari, Majlinda A1 - Novotny, Ivan A1 - Rehacek, Vlastimil A1 - Tvarozek, Vladimir A1 - Wolfbeis, Otto S. T1 - Self-assembled monolayers as selective filters for chemical sensors T2 - Nanotechnology N2 - The lateral electrical conductivity of gold layers with thickness in the nanometre range was found to decrease due to adsorption of mercury, water, iodine and sulphuric compounds onto the gold surface. A self-assembled monolayer of hexadecanethiol, deposited on the gold surface, blocked the effects of water and volatile sulphuric compounds on the lateral conductivity of the gold layers, but does not block the effects of iodine and mercury. The results indicate a selective permeability of hexadecanethiol monolayers for mercury vapour, thus providing a method for development of ultrathin filters for chemical sensors. Y1 - 2002 SN - 0957-4484 VL - 13 IS - 2 SP - 175 EP - 178 ER - TY - GEN A1 - Cherny, Vladimir V. A1 - Stozhkova, I. N. A1 - Mirsky, Vladimir M. A1 - Yastrebova, T. N. A1 - Sokolov, Valeri S. T1 - Modification of lipid bilayer properties due to sodium hypochlorite. II. Effect on model lipid systems T2 - Biological membranes Y1 - 1992 SN - 0233-4755 VL - 9 IS - 1 SP - 66 EP - 73 ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Cherny, Vladimir V. A1 - Sokolov, Valeri S. A1 - Markin, Vladislav S. T1 - Electrostatic assay of phospholipase A activity: an application of the second harmonic method of monitoring membrane boundary potentials T2 - Journal of Biochemical and Biophysical Methods N2 - To evaluate phospholipase A activity a new assay is suggested. This assay is based on the recording of boundary potential changes of the planar bilayer lipid membrane during enzymatic hydrolysis of lipids. To register these changes, a second harmonic method is used. Sensitivity of the assay is about 0.0002 units/ml regardless of the impurities that may be present in the samples. One analysis takes about 5 min. Y1 - 1990 SN - 0165-022X SN - 1872-857X VL - 21 IS - 4 SP - 277 EP - 284 ER - TY - GEN A1 - Cherny, Vladimir V. A1 - Mirsky, Vladimir M. A1 - Sokolov, Valeri S. A1 - Markin, Vladislav S. T1 - Determination of the phospholipase A activity T2 - Biochemistry Y1 - 1990 VL - 55 IS - 3 SP - 445 EP - 450 ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Cherny, Vladimir V. A1 - Sokolov, Valeri S. A1 - Markin, Vladislav S. T1 - Electrode for determination of the phospholipase activity based on the lipid membrane T2 - Soviet electrochemistry Y1 - 1989 VL - 25 SP - 1336 EP - 1340 ER - TY - GEN A1 - Cherny, Vladimir V. A1 - Mirsky, Vladimir M. A1 - Sokolov, Valeri S. A1 - Markin, Vladislav S. T1 - BLM as an enzyme-sensitive electrode: determination of the phospholipase activity T2 - Bioelectrochemistry and Bioenergetics Y1 - 1989 SN - 0302-4598 VL - 21 IS - 3 SP - 373 EP - 378 ER - TY - GEN A1 - Shishkanova, Tatiana V. A1 - Havlik, Martin A1 - Král, Vladimír A1 - Kopecký, Dušan A1 - Matějka, Pavel A1 - Dendisová, Marcela A1 - Mirsky, Vladimir M. T1 - Amino-substituted Tröger’s base: electrochemical polymerization and characterization of the polymer film T2 - Electrochimica Acta N2 - The amino-substituted coumarin derivative of Tröger’s base (CTB) was electrochemically polymerized on the surface of gold electrodes. The obtained polymer films were characterized by SEM, Raman spectroscopy, cyclic voltammetry and in-situ conductometry. Spectroscopic analysis indicates that the polymerization of CTB takes place via oxidation of the aniline fragment of CTB molecule, similar to the polymerization of aniline. The similarity of the polymer backbone to that of the polyaniline is also confirmed by the influence of potential on the film’s conductivity, however the conductivity of the new material is in principle lower than that of polyaniline. SEM shows that the polymer forms a thin layer with a thickness of ~ 100 nm. Possible applications of the new material as an artificial receptor for aromatic analytes containing carboxy-group are proposed. KW - electrochemical polymerization KW - polymer film KW - polymeric Tröger’s base KW - nitrogen heterocycle KW - spectroscopic characterization Y1 - 2017 U6 - https://doi.org/10.1016/j.electacta.2016.12.061 SN - 0013-4686 VL - 224 SP - 439 EP - 445 ER - TY - GEN A1 - Vasjari, Majlinda A1 - Shirshov, Yuri M. A1 - Samoylov, Anton V. A1 - Mirsky, Vladimir M. T1 - SPR investigation of mercury reduction and oxidation on thin gold electrodes T2 - Journal of Electroanalytical Chemistry N2 - Mercury reduction and oxidation on ultrathin gold electrodes was investigated by simultaneous voltammetry and surface plasmon resonance (SPR). The angle dependencies of SPR signal were analyzed quantitatively. The results indicate partially reversible formation of amalgam with optical properties in between of optical properties of mercury and gold. Up to 12% content of mercury in the gold layer is suggested. KW - Surface plasmon resonance KW - Electrochemical SPR KW - Simultaneous SPR and voltammetry KW - Mercury KW - Amalgam Y1 - 2007 U6 - https://doi.org/10.1016/j.jelechem.2007.03.019 SN - 1572-6657 VL - 605 IS - 1 SP - 73 EP - 76 ER - TY - GEN A1 - Samoylov, Anton V. A1 - Mirsky, Vladimir M. A1 - Hao, Qingli A1 - Swart, Claudia A1 - Shirshov, Yuri M. A1 - Wolfbeis, Otto S. T1 - Nanometer-thick SPR sensor for gaseous HCl T2 - Sensors and Actuators B: Chemical N2 - The optical properties of electrochemically polymerized N-methylaniline are changed in the presence of gaseous hydrogen chloride. This effect was used for preparation of chemical sensors with transduction based on surface plasmon resonance. The interaction of hydrogen chloride with a two nm layer of poly-(N-methylaniline) on gold leads to the shift of the surface plasmon resonance. The analysis of the resonance spectra demonstrates that the effect is caused by an increase of the imaginary component of the refractive index; a minor contribution is also provided by film condensation leading to an increase of the real component of the refractive index and a decrease in the thickness. The effect is selective and quasi-reversible. The concentration dependence of the gas effect obeys Langmuir's adsorption isotherm with a reciprocal value of the binding constant of 850 ± 160 ppm. KW - Gas sensor KW - HCI sensors KW - Surface plasmon resonance KW - Electropolymerization KW - Poly-(N-methyl-aniline) KW - Fire detector Y1 - 2005 U6 - https://doi.org/10.1016/j.snb.2004.08.029 SN - 0925-4005 VL - 106 IS - 1 SP - 369 EP - 372 ER - TY - GEN A1 - Zynio, Stepan A. A1 - Samoylov, Anton V. A1 - Surovtseva, Elena R. A1 - Mirsky, Vladimir M. A1 - Shirshov, Yuri M. T1 - Bimetallic layers increase sensitivity of affinity sensors based on surface plasmon resonance T2 - Sensors N2 - Two metals are used in resonant layers for chemical sensors based on surface plasmon resonance (SPR) - gold and silver. Gold displays higher shift of the resonance angle to changes of ambient refraction index and is chemically stable. Silver posses narrower resonance curve thus providing a higher signal/noise ratio of SPR chemical sensors, but has a poor chemical stability. A new structure of resonant metallic film based on bimetallic silver/gold layers (gold as an outer layer) is suggested. It combines advantages of both gold and silver resonant layers. Bimetallic resonant films display so high shift of resonance angle on changes of ambient refraction index as gold films, but show narrower resonance curve, thus providing a higher signal / noise ratio. Additionally, the outer gold layer protects silver against oxidation. KW - Surface plasmon resonance KW - Biosensor KW - Sensitivity KW - Signal / noise ratio Y1 - 2002 U6 - https://doi.org/10.3390/s20200062 SN - 1424-8220 VL - 2 IS - 2 SP - 62 EP - 70 ER - TY - GEN A1 - Bajari, Tarek M. A1 - Lindstedt, Ken A. A1 - Riepl, Michael A1 - Mirsky, Vladimir M. A1 - Nimpf, Johannes A1 - Wolfbeis, Otto S. A1 - Dresel, Hans A. A1 - Bautz, Ekkehard K. F. A1 - Schneider, Wolfgang Johann T1 - A minimal binding domain of the low density lipoprotein receptor gene family T2 - Biological Chemistry KW - Ligand binding KW - Low density lipoprotein KW - Phage display KW - Receptor KW - Oocyte Y1 - 1998 U6 - https://doi.org/10.1515/bchm.1998.379.8-9.1053 SN - 1437-4315 VL - 379 IS - 8-9 SP - 1053 EP - 1062 ER -