TY - GEN A1 - Herzog, Natalie A1 - Hansen, Max A1 - Miethbauer, Sebastian A1 - Schmidtke, Kai-Uwe A1 - Anderer, Ursula A1 - Lupp, Amelie A1 - Sperling, Sebastian A1 - Seehofer, Daniel A1 - Damm, Georg A1 - Scheibner, Katrin A1 - Küpper, Jan-Heiner T1 - Primary-like human hepatocytes genetically engineered to obtain proliferation competence display hepatic differentiation characteristics in monolayer and organotypical spheroid cultures T2 - Cell Biology International N2 - Primary human hepatocytes are in great demand during drug development and in hepatology. However, both scarcity of tissue supply and donor variability of primary cells create a need for the development of alternative hepatocyte systems. By using a lentivirus vector system to transfer coding sequences of Upcyte® proliferation genes, we generated non-transformed stable hepatocyte cultures from human liver tissue samples. Here, we show data on newly generated proliferation-competent HepaFH3 cells investigated as conventional two-dimensional monolayer and as organotypical three-dimensional (3D) spheroid culture. In monolayer culture, HepaFH3 cells show typical cobblestone-like hepatocyte morphology and anchorage-dependent growth for at least 20 passages. Immunofluorescence staining revealed that characteristic hepatocyte marker proteins cytokeratin 8, human serum albumin, and cytochrome P450 (CYP) 3A4 were expressed. Quantitative real-time PCR analyses showed that expression levels of analyzed phase I CYP enzymes were at similar levels compared to those of cultured primary human hepatocytes and considerably higher than in the liver carcinoma cell line HepG2. Additionally, transcripts for phase II liver enzymes and transporter proteins OATP-C, MRP2, Oct1, and BSEP were present in HepaFH3. The cells produced urea and converted model compounds such as testosterone, diclofenac, and 7-OH-coumarin into phases I and II metabolites. Interestingly, phases I and II enzymes were expressed at about the same levels in convenient monolayer cultures and complex 3D spheroids. In conclusion, HepaFH3 cells and related primary-like hepatocyte lines seem to be promising tools for in vitro research of liver functions and as test system in drug development and toxicology analysis. KW - cytochrome P450 enzyme Y1 - 2016 UR - http://onlinelibrary.wiley.com/doi/10.1002/cbin.10574/full U6 - https://doi.org/10.1002/cbin.10574 SN - 1095-8355 VL - 40 IS - 3 SP - 341 EP - 353 ER - TY - GEN A1 - Herzog, Natalie A1 - Hansen, Max A1 - Miethbauer, Sebastian A1 - Schmidtke, Kai-Uwe A1 - Anderer, Ursula A1 - Lupp, Amelie A1 - Sperling, Sebastian A1 - Seehofer, Daniel A1 - Damm, Georg A1 - Scheibner, Katrin A1 - Küpper, Jan-Heiner T1 - Primary like human hepatocytes genetically engineered to obtain proliferation competence display liver biotransformation activity in 2D and 3D culture systems T2 - Cell Biology International Y1 - 2016 SN - 1095-8355 SN - 1065-6995 VL - 40 IS - 3 SP - 341 EP - 353 ER - TY - GEN A1 - Lehmann, Mario A1 - Martin, Frank A1 - Mannigel, Kerstin A1 - Kaltschmidt, Kristin A1 - Sack, Ulrich A1 - Anderer, Ursula T1 - Three-dimensional scaffold-free fusion culture: the way to enhanced chondrogenesis of in vitro propagated human articular chondrocytes T2 - European Journal of Histochemistry KW - in vitro cartilage KW - 3-D cell culture KW - fusion culture technique KW - tissue engineering KW - cell differentiation KW - extracellular matrix KW - immunohistochemistry Y1 - 2013 U6 - https://doi.org/10.4081/ejh.2013.e31 SN - 2038-8306 SN - 1121-760X VL - 57 IS - 4 SP - 205 EP - 215 ER - TY - GEN A1 - Martin, Frank A1 - Lehmann, Mario A1 - Schläger, Peter A1 - Sack, Ulrich A1 - Anderer, Ursula T1 - Differentiation capacity of chondrocytes in microtissues depends on TGF-ß subtyp T2 - Journal of biochips & tissue chips : JBTC KW - microtissues KW - differentiation of chondrocytes KW - TGF-β subtypes KW - BMP-7 Y1 - 2012 U6 - https://doi.org/10.4172/2153-0777.S2-002 SN - 2153-0777 N1 - Special Issue Article VL - S2 IS - 002 ER - TY - GEN A1 - Knütter, Ilka A1 - Hiemann, Rico A1 - Brumma, Therese A1 - Büttner, Thomas A1 - Großmann, Kai A1 - Cusini, Marco A1 - Pregnolato, Francesca A1 - Borghi, Maria Orietta A1 - Anderer, Ursula A1 - Conrad, Karsten A1 - Reinhold, Dirk A1 - Roggenbuck, Dirk A1 - Csernok, Elena T1 - Automated interpretation of ANCA patterns - a new approach in the serology of ANCA-associated vasculitis T2 - Arthritis Research & Therapy KW - ANCA-associated vasculitis KW - serology KW - pattern recognition algorithms KW - discrimination and classification of cytoplasmic ANCA (C-ANCA) and perinuclear ANCA (P-ANCA) KW - automated interpretation of ANCA IIF Y1 - 2012 UR - http://arthritis-research.com/content/14/6/R271 U6 - https://doi.org/10.1186/ar4119 SN - 1465-9913 SN - 1478-6354 VL - 14 ER - TY - GEN A1 - Lutter, Anne-Helen A1 - Scholka, Jenny A1 - Richter, Heiko A1 - Anderer, Ursula T1 - Applying XTT, WST-1, and WST-8 to human chondrocytes: A comparison of membrane-impermeable tetrazolium salts in 2D and 3D cultures T2 - Clinical Hemorheology and Microcirculation KW - chondrogenic differentiation Y1 - 2017 U6 - https://doi.org/10.3233/CH-179213 SN - 1875-8622 SN - 1386-0291 VL - 67 IS - 3-4 SP - 327 EP - 342 ER - TY - GEN A1 - Lutter, Anne-Helen A1 - Hempel, Ute A1 - Anderer, Ursula A1 - Dieter, Peter T1 - Biphasic influence of PGE2 on the resorption activity of osteoclast-like cells derived from human peripheral blood monocytes and mouse RAW264.7 T2 - Prostaglandins, Leukotrienes and Essential Fatty Acids KW - osteoclastic differentiation Y1 - 2016 U6 - https://doi.org/10.1016/j.plefa.2016.03.017 SN - 1532-2823 SN - 0952-3278 VL - 111 SP - 1 EP - 7 ER - TY - GEN A1 - Martin, Frank A1 - Lehmann, Mario A1 - Sack, Ulrich A1 - Anderer, Ursula T1 - In vitro development of personalized cartilage microtissues uncovers an individualized differentiation capacity of human chondrocytes T2 - Experimental Biology and Medicine KW - Articular cartilage KW - 3D culture KW - microtissue KW - scaffold-free KW - personalized differentiation KW - responder/non-responder KW - personalized diagnostic platform Y1 - 2017 U6 - https://doi.org/10.1177/1535370217728498 SN - 1535-3702 VL - 242 IS - 18 SP - 1746 EP - 1756 ER - TY - CHAP A1 - Anderer, Ursula A1 - Savkovic, Vuc ED - Sack, Ulrich T1 - Zelltherapien in der Regenerativen Medizin T2 - Zelluläre Diagnostik und Therapie KW - Zellbasierte Verfahren zur Regeneration von Gelenkknorpel KW - Mesenchymale Stammzellen in der Regenerativen Therapie KW - Biomaterialien - Strukturgeber und physiologischer Impulsgeber KW - Regenerative Zelltherapien für die Haut KW - Regenerative Zelltherapien für das Nervensystem Y1 - 2016 SN - 978-3-11-034406-6 SP - 291 EP - 341 PB - Walter de Gruyter CY - Berlin [u.a.] ET - 1. Auflage ER - TY - CHAP A1 - Martin, Frank A1 - Lehmann, Mario A1 - Anderer, Ursula ED - Daskalaki, Adriani T1 - Generation of scaffold free 3-D cartilage-like microtissues from human chondrocytes T2 - Medical Advancements in Aging and Regenerative Technologies: Clinical Tools and Applications KW - Cartilage Repair Strategies KW - Engineering cartilage-like microtissues without scaffolds KW - Redifferentiation of chondrocytes in a three-dimensional environment KW - S100 protein as marker for chondrogenic development Y1 - 2013 SN - 978-1-4666-2506-8 SP - 169 EP - 194 PB - IGI Global CY - Hershey, PA, USA ER -