TY - GEN A1 - Lutter, Anne-Helen A1 - Ecke, Annemarie A1 - Scholka, Jenny A1 - Waldeck, Peter A1 - Tretter, Tim A1 - Anderer, Ursula T1 - Differentiation of human chondrocytes under the influence of microalgae-based exopolysaccharides T2 - 38. Jahrestagung der Deutschen Gesellschaft für Klinische Mikrozirkulation und Hämorheologie : Programm, 22. und 23. November 2019, Braunschweig N2 - BACKGROUND: Exopolysaccharides are formed by microalgae in response to adverse conditions and are found mainly as a coating around the cells. The protective function of EPS plays an important role in the survival of microalgae in polluted habitats. So far, the particular biological and physico-chemical properties of EPS have not been adequately investigated and their effect on cells such as human chondrocytes has not been tested. Microalgae in particular offer an ideal platform for the production of dietary supplements or drugs that enable new therapeutic approaches. METHODS: To investigate the influence of EPS on human chondrocytes, cytotoxicity and differentiation potential were analyzed in 3D cultures. RESULTS: We were able to demonstrate a positive, but donor-specific influence of EPS on the differentiation of human chondrocytes. Furthermore, the EPS showed no cytotoxic effects in a range of up to 250 µg EPS per ml medium. CONCLUSION: EPS have the potential to positively influence the differentiation of human chondrocytes. Further experiments are necessary to analyze the donor-specific effect. Y1 - 2019 UR - https://www.orbera.de/referenzen/DGKMH_2019/pdf_DGKMH_2019.pdf ER -