TY - GEN A1 - Li, Chouyang A1 - Rezania, Simin A1 - Kammerer, Sarah A1 - Sokolowski, Armin Andreas A1 - Devaney, Trevor Thomas Joseph A1 - Gorischek, Astrid A1 - Jahn, Stephan Wenzel A1 - Hackl, Hubert A1 - Groschner, Klaus A1 - Windpassinger, Christian A1 - Malle, Ernst A1 - Bauernhofer, Thomas A1 - Schreibmayer, Wolfgang T1 - Piezo1 forms mechanosensitive ion channels in the human MCF-7 breast cancer cell line T2 - Scientific Reports N2 - Mechanical interaction between cells - specifically distortion of tensional homeostasis-emerged as an important aspect of breast cancer genesis and progression. We investigated the biophysical characteristics of mechanosensitive ion channels (MSCs) in the malignant MCF-7 breast cancer cell line. MSCs turned out to be the most abundant ion channel species and could be activated by negative pressure at the outer side of the cell membrane in a saturable manner. Assessing single channel conductance (GΛ) for different monovalent cations revealed an increase in the succession: Li⁺ < Na⁺ < K⁺ ≈Rb⁺ ≈ Cs⁺. Divalent cations permeated also with the order: Ca²⁺ < Ba²⁺. Comparison of biophysical properties enabled us to identify MSCs in MCF-7 as ion channels formed by the Piezo1 protein. Using patch clamp technique no functional MSCs were observed in the benign MCF-10A mammary epithelial cell line. Blocking of MSCs by GsMTx-4 resulted in decreased motility of MCF-7, but not of MCF-10A cells, underscoring a possible role of Piezo1 in invasion and metastatic propagation. The role of Piezo1 in biology and progression of breast cancer is further substantiated by markedly reduced overall survival in patients with increased Piezo1 mRNA levels in the primary tumor. Y1 - 2015 U6 - https://doi.org/10.1038/srep08364 SN - 2045-2322 VL - 5 ER - TY - CHAP A1 - Berger, Ulrich A1 - Schäfer, Stephan A1 - Schöttke, Dirk A1 - Kämpfe, Thomas A1 - Matura, Dominik T1 - Collaborating Robots in a Museum Environment - Modular Systems for 3D Documentation T2 - 20th Conference on Emerging Technologies & Factory Automation (ETFA), 8-11 Sept. 2015 Luxembourg Y1 - 2015 SN - 978-1-4673-7929-8 U6 - https://doi.org/10.1109/ETFA.2015.7301610 PB - IEEE CY - Piscataway, NJ ER - TY - CHAP A1 - Utgenannt, Stephan A1 - Hansen, Felix A1 - Klepel, Olaf A1 - Jarczewski, Sebastian A1 - Wach, Anna A1 - Kustrowski, Piotr A1 - Erler, Thomas A1 - Bron, Michael T1 - A modular concept for the preparation of carbon based catalysts T2 - Carbon 2015 : innovation with carbon materials; the Annual World Conference on Carbon, Dresden, Germany, July 12 - 17, 2015 Y1 - 2015 SP - S. 294 PB - Deutsche Keramische Gesellschaft e.V. CY - Köln ER -