TY - GEN A1 - Rezania, Simin A1 - Kammerer, Sarah A1 - Li, Chouyang A1 - Steinecker-Frohnwieser, Bibiane A1 - Gorischek, Astrid A1 - Devaney, Trevor Thomas Joseph A1 - Verheyen, Sarah A1 - Passegger, Christina A1 - Ghaffari Tabrizi-Wizsy, Nassim A1 - Hackl, Hubert A1 - Platzer, Dieter A1 - Zarnani, A.H. A1 - Malle, Ernst A1 - Jahn, Stephan Wenzel A1 - Bauernhofer, Thomas A1 - Schreibmayer, Wolfgang T1 - Overexpression of KCNJ3 gene splice variants affects vital parameters of the malignant breast cancer cell line MCF-7 in an opposing manner T2 - BMC Cancer N2 - Background Overexpression the KCNJ3, a gene that encodes subunit 1 of G-protein activated inwardly rectifying K+ channel (GIRK1) in the primary tumor has been found to be associated with reduced survival times and increased lymph node metastasis in breast cancer patients. Methods In order to survey possible tumorigenic properties of GIRK1 overexpression, a range of malignant mammary epithelial cells, based on the MCF-7 cell line that permanently overexpress different splice variants of the KCNJ3 gene (GIRK1a, GIRK1c, GIRK1d and as a control, eYFP) were produced. Subsequently, selected cardinal neoplasia associated cellular parameters were assessed and compared. ResultsAdhesion to fibronectin coated surface as well as cell proliferation remained unaffected. Other vital parameters intimately linked to malignancy, i.e. wound healing, chemoinvasion, cellular velocities / motilities and angiogenesis were massively affected by GIRK1 overexpression. Overexpression of different GIRK1 splice variants exerted differential actions. While GIRK1a and GIRK1c overexpression reinforced the affected parameters towards malignancy, overexpression of GIRK1d resulted in the opposite. Single channel recording using the patch clamp technique revealed functional GIRK channels in the plasma membrane of MCF-7 cells albeit at very low frequency. DiscussionWe conclude that GIRK1d acts as a dominant negative constituent of functional GIRK complexes present in the plasma membrane of MCF-7 cells, while overexpression of GIRK1a and GIRK1c augmented their activity. The core component responsible for the cancerogenic action of GIRK1 is apparently presented by a segment comprising aminoacids 235–402, that is present exclusively in GIRK1a and GIRK1c, but not GIRK1d (positions according to GIRK1a primary structure). Conclusions The current study provides insight into the cellular and molecular consequences of KCNJ3 overexpression in breast cancer cells and the mechanism upon clinical outcome in patients suffering from breast cancer. Y1 - 2016 U6 - https://doi.org/10.1186/s12885-016-2664-8 SN - 1471-2407 VL - 16 ER - TY - CHAP A1 - Simon, Sylvio A1 - Meißner, Thomas A1 - Wichmann, Steffen A1 - Hernschier, Stephan A1 - Weist, Michael T1 - Energieeffizienz in der Maschinenkonstruktion T2 - Proceedings of the International Academic Forum AMO-SPITSE-NESEFF, 21 AMO Conference, Smolensk 2016 N2 - Die konsequente Anwendung von Gestaltungsrichtlinien im Bezug auf die Masse der verwendeten Maschinenelemente hilft energieeffiziente Produkte zu gestalten. Maschinenelemente wie Schrauben, berührungslose Dichtungen oder Radialwellendichtringe können dabei einen entsprechenden Beitrag leisten. KW - Leichtbau KW - Maschinenkonstruktuon KW - Energieeffizienz Y1 - 2016 SN - 978-5-91412-313-7 SP - S. 125 PB - Izdat. Universum CY - Moscow [u.a.] ER - TY - CHAP A1 - Hernschier, Stephan A1 - Rieder, Thomas A1 - Schneider, Robert A1 - Magister, Jan A1 - Simon, Sylvio ED - Simon, Sylvio T1 - Aktuelle Untersuchungen an Tragrollen T2 - 3. Ingenieurtag 2016 der Fakultät Maschinenbau, Elektro- und Energiesysteme - GUS und Osteuropatag - NESEFF-Netzwerktreffen 2016, Tagungsband N2 - Die Minderung der Geräuschemission von Hochleistungsgurtförderanlagen in Tagebauen stellt in Verbindung mit höheren Anforderungen des Umweltschutzes und der Akzeptanz in der Bevölkerung ein Problem dar. Zum einen gilt es Mensch und Umwelt vor Lärm zu schützen und zum anderen ist es für den Betreiber schwierig, die gesetzlichen Auflagen für den Betrieb solcher Anlagen zu erfüllen. Untersuchungen haben gezeigt, dass Tragrollen die Hauptschallquellen dieser Gurtförderanlagen sind. Im Rahmen einer Baugruppenprüfung werden in Kooperation mit der Lausitz Energie Bergbau AG (LEAG) akustische und mechanische Kenngrößen von Tragrollen auf einem Prüfstand (TPS) an der Brandenburgischen Technischen Universität Cottbus-Senftenberg untersucht. KW - Tragrollen KW - Gurtförderanlage KW - Lärm KW - Schallquelle Y1 - 2016 SN - 978-3-940471-28-4 SP - 157 EP - 161 PB - Brandenburgische Techn. Univ. CY - Cottbus-Senftenberg ER - TY - CHAP A1 - Rößler, Thomas A1 - Rogge, Stephan A1 - Hentschel, Christian T1 - Optimized light output for passive stereoscopic projection in VR-systems T2 - IEEE 6th International Conference on Consumer Electronics - Berlin (ICCE-Berlin), 5-7 Sept. 2016 Y1 - 2016 UR - http://ieeexplore.ieee.org/document/7684769/ SN - 978-1-5090-2096-6 SN - 978-1-5090-2097-3 U6 - https://doi.org/10.1109/ICCE-Berlin.2016.7684769 PB - IEEE CY - Piscataway, NJ ER - TY - CHAP A1 - Repins, Ingrid A1 - Mansfield, Lorelle M. A1 - Kanevce, Ana A1 - Jensen, Soren A. A1 - Kuciauskas, Darius A1 - Glynn, Stephen A1 - Barnes, Teresa A1 - Metzger, Wyatt A1 - Burst, James A1 - Jiang, Chun-Sheng A1 - Harvey, Steve A1 - Dippo, Patricia A1 - Teeter, Glenn A1 - Perkins, C. A1 - Egaas, Brian A1 - Zakutayev, Andriy A1 - Alsmeier, Jan Hendrik A1 - Lußky, Thomas A1 - Korte, Lars A1 - Wilks, Regan G. A1 - Bär, Marcus A1 - Yan, Yanfa A1 - Lany, Stephan A1 - Zawadzki, Pawel A1 - Zawadzki, Pawel A1 - Park, Ji-Sang A1 - Wei, Suhuai T1 - Wild band edges: The role of bandgap grading and band-edge fluctuations in high-efficiency chalcogenide devices T2 - 2016 IEEE 43rd Photovoltaic Specialists Conference (PVSC), Portland, OR, USA, June 5-10, 2016 Y1 - 2016 SN - 978-1-5090-2724-8 SP - 309 EP - 314 PB - IEEE CY - Piscataway, NJ ER - TY - CHAP A1 - Handick, Evelyn A1 - Reinhard, Patrick A1 - Wilks, Regan G. A1 - Pianezzi, Fabian A1 - Duarte, Roberto Felix A1 - Gorgoi, Mihaela A1 - Kunze, Thomas A1 - Bücheler, Stephan A1 - Tiwari, Ayodhya N. A1 - Bär, Marcus T1 - NaF/KF post-deposition treatments and their influence on the structure of Cu(In,Ga)Se₂ absorber surfaces T2 - IEEE 43rd Photovoltaic Specialists Conference (PVSC), 5-10 June 2016 Y1 - 2016 SN - 978-1-5090-2724-8 U6 - https://doi.org/10.1109/PVSC.2016.7749400 SP - 17 EP - 21 PB - IEEE CY - Piscataway, NJ ER - TY - CHAP A1 - Berger, Ulrich A1 - Schäfer, Stephan A1 - Schottke, Dirk A1 - Kampfe, Thomas A1 - Tauber, Bernd A1 - Krönke, Dietrich T1 - Increasing the flexibility of manufacturing: A service-oriented approach in automation T2 - 21st International Conference on Emerging Technologies and Factory Automation (ETFA), 6-9 Sept. 2016, Berlin N2 - In addition to the already required functionality, future production systems should consider the requirements of flexible and demand-oriented resource utilization. Those are not only related on fields of energy, but also material-efficient and time-efficient use of available means of production. Modular system architectures and modular solutions facilitate the planning and implementation of these requirements. Using service-oriented architectures in automation allow an approach for new or modified system components that can be integrated in the available plant environment without a high additional investment of project engineering. This aspect is interesting for companies with manageable machinery, which are able to react immediately and flexible to changes. Service-oriented solutions include among other things the identification, classification and synchronization of possible services. The paper will show general prerequisites for an implementation of a service-oriented architecture in a module-based test field. Y1 - 2016 SN - 978-1-5090-1314-2 U6 - https://doi.org/10.1109/ETFA.2016.7733673 PB - IEEE CY - Piscataway, NJ ER - TY - CHAP A1 - Berger, Ulrich A1 - Schäfer, Stephan A1 - Schöttke, Dirk A1 - Kämpfe, Thomas A1 - Krönke, Dietrich T1 - Fertigungsanlagen flexibel gestalten – Konzept einer plattform- und serviceorientierten Lösung T2 - Entwurf komplexer Automatisierungssysteme, EKA 2016, 24.-25.05.2016, 14. Fachtagung mit Tutorium Y1 - 2016 SN - 978-3-944722-35-1 SP - 1 EP - 12 PB - Institut für Automation und Kommunikation e.V. CY - Magdeburg ER - TY - GEN A1 - Berger, Ulrich A1 - Schäfer, Stephan A1 - Schöttke, Dirk A1 - Kämpfe, Thomas A1 - Krönke, Dietrich A1 - Tauber, Bernd T1 - Flexible Erweiterung von Arbeitsräumen T2 - atp edition Y1 - 2016 SN - 2364-3137 SN - 0178-2320 VL - 58 IS - 7-8 SP - 58 EP - 67 ER - TY - GEN A1 - Kammerer, Sarah A1 - Jahn, Stephan Wenzel A1 - Winter, Elke A1 - Eidenhammer, Sylvia A1 - Rezania, Simin A1 - Regitnig, Peter A1 - Pichler, Martin A1 - Schreibmayer, Wolfgang A1 - Bauernhofer, Thomas T1 - Critical evaluation of KCNJ3 gene product detection in human breast cancer: MRNA in situ hybridisation is superior to immunohistochemistry T2 - Journal of Clinical Pathology N2 - Increased expression levels of KCNJ3 have been correlated with lymph node metastases and poor prognosis in patients with breast cancer, suggesting a prognostic role of KCNJ3. We aimed to establish protocols for the detection of KCNJ3 in formalin-fixed, paraffin-embedded (FFPE) breast cancer tissue. Several antibodies were tested for sensitivity and specificity by western blot, followed by optimisation of the immunohistochemistry (IHC) procedure and establishment of KCNJ3 mRNA in situ hybridisation (ISH). Methods were validated by processing 15 FFPE breast cancer samples for which microarray data were available. Spearman's rank correlation analysis resulted in borderline significant correlation for IHC versus ISH (rS: 0.625; p<0.05) and IHC versus microarray (rS: 0.668; p<0.01), but in significant correlation for ISH versus microarray (rS: 0.861; p<0.001). The ISH method was superior to IHC, regarding robustness, sensitivity and specificity and will aid to further study expression levels of KCNJ3 in both malignant and physiological conditions. Y1 - 2016 U6 - https://doi.org/10.1136/jclinpath-2016-203798 SN - 1472-4146 SN - 0021-9746 VL - 69 IS - 12 SP - 1116 EP - 1121 ER - TY - GEN A1 - Kammerer, Sarah A1 - Sokolowski, Armin Andreas A1 - Hackl, Hubert A1 - Platzer, Dieter A1 - Jahn, Stephan Wenzel A1 - El-Heliebi, Amin A1 - Schwarzenbacher, Daniela A1 - Stiegelbauer, Verena A1 - Pichler, Martin A1 - Rezania, Simin A1 - Fiegl, Heidelinde A1 - Peintinger, Florentia A1 - Regitnig, Peter A1 - Höfler, Gerald A1 - Schreibmayer, Wolfgang A1 - Bauernhofer, Thomas T1 - KCNJ3 is a new independent prognostic marker for estrogen receptor positive breast cancer patients T2 - OncoTarget : open access impact journal N2 - Numerous studies showed abnormal expression of ion channels in different cancer types. Amongst these, the potassium channel gene KCNJ3 (encoding for GIRK1 proteins) has been reported to be upregulated in tumors of patients with breast cancer and to correlate with positive lymph node status. We aimed to study KCNJ3 levels in different breast cancer subtypes using gene expression data from the TCGA, to validate our findings using RNA in situ hybridization in a validation cohort (GEO ID GSE17705), and to study the prognostic value of KCNJ3using survival analysis. In a total of > 1000 breast cancer patients of two independent data sets we showed a) that KCNJ3 expression is upregulated in tumor tissue compared to corresponding normal tissue (p < 0.001), b) that KCNJ3 expression is associated with estrogen receptor (ER) positive tumors (p < 0.001), but that KCNJ3 expression is variable within this group, and c) that ER positive patients with high KCNJ3 levels have worse overall (p < 0.05) and disease free survival probabilities (p < 0.01), whereby KCNJ3 is an independent prognostic factor (p <0.05). In conclusion, our data suggest that patients with ER positive breast cancer might be stratified into high risk and low risk groups based on the KCNJ3 levels in the tumor. Y1 - 2016 U6 - https://doi.org/10.18632/oncotarget.13224 VL - 7 IS - 51 SP - 84705 EP - 84717 ER -