TY - GEN A1 - Berger, Ulrich A1 - Creutznacher, Thomas A1 - Lepratti, Raffaello A1 - Lamparter, Steffen T1 - Flexible Produktionssysteme in der Praxis T2 - Industrie 4.0 Management Y1 - 2015 SN - 2364-9208 IS - 6 ER - TY - GEN A1 - Creutznacher, Thomas A1 - Berger, Ulrich A1 - Lepratti, Raffaello A1 - Lamparter, Steffen T1 - The transformable factory: adapting automotive production capacities T2 - Procedia CIRP Y1 - 2016 U6 - https://doi.org/10.1016/j.procir.2015.12.138 SN - 2212-8271 N1 - Research and Innovation in Manufacturing: Key Enabling Technologies for the Factories of the Future - Proceedings of the 48th CIRP Conference on Manufacturing Systems VL - 41 SP - 171 EP - 176 ER - TY - GEN A1 - Allertz, Peter J. A1 - Berger, Steffen A1 - Sellenk, Grit A1 - Dittmer, Christin A1 - Dietze, Marco A1 - Stahmann, Klaus-Peter A1 - Salchert, Katrin T1 - Approaching Immobilization of Enzymes onto Open Porous Basotect® T2 - Catalysts N2 - For the first time, commercial macroporous melamine formaldehyde foam Basotect® (BT) was used as a basic carrier material for both adsorptive and covalent enzyme immobilization. In order to access inherent amino groups, the Basotect® surface was pretreated with hydrochloric acid. The resulting material revealed 6 nmol of superficial amino groups per milligram Basotect®. Different optimized strategies for tethering the laccase from Trametes versicolor and the lipase from Thermomyces lanuginosus onto the pre-treated Basotect® surface were studied. Particularly, for covalent immobilization, two different strategies were pursued: lipase was tethered via a cross-linking method using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide, and laccase was bound after functionalizing Basotect® with hydrophilic copolymer poly(ethylene-alt-maleic anhydride) (PEMA). Prior to laccase immobilization, the PEMA coating of Basotect® was verified by ATR-FTIR analysis. Subsequent quantification of available high-reactive PEMA anhydride moieties revealed an amount of 1028 ± 73 nmol per mg Basotect®. The surface-bound enzyme amounts were quantified as 4.1–5.8 μg per mg Basotect®. A theoretical surface-covered enzyme mass for the ideal case that an enzyme monolayer was immobilized onto the Basotect® surface was calculated and compared to the amount of adsorptive and covalently bound enzymes before and after treatment with SDS. Furthermore, the enzyme activities were determined for the different immobilization approaches, and the stability during storage over time and against sodium dodecyl sulfate treatment was monitored. Additionally, PEMA-BT-bound laccase was tested for the elimination of anthropogenic micropollutant bisphenol A from contaminated water in a cost-effective and environmentally-friendly way and resulted in a degradation rate higher than 80%. KW - Basotect® KW - Enzyme immobilization KW - Laccase KW - Lipase KW - PEMA KW - EDC KW - Bisphenol A Y1 - 2017 U6 - https://doi.org/10.3390/catal7120359 SN - 2073-4344 VL - 7 IS - 12 ER - TY - CHAP A1 - Creutznacher, Thomas A1 - Berger, Ulrich A1 - Lepratti, Raffaello A1 - Lamparter, Steffen T1 - Situationsadaptive Fertigung: Steigerung der Flexibilität automatisierter Fertigungsprozesse in der Automobilindustrie T2 - Automation 2014, 15. Branchentreff der Mess- und Automatisierungstechnik, Kongresshaus Baden-Baden, 01. und 02. Juli 2014 Y1 - 2014 SN - 978-3-18-092231-7 SP - 339 EP - 354 PB - VDI-Verlag CY - Düsseldorf ER - TY - CHAP A1 - Berger, Ulrich A1 - Creutznacher, Thomas A1 - Lepratti, Raffaello A1 - Lamparter, Steffen T1 - Optimierung von Fertigungsprozessen der Automobilindustrie durch PLM-MES-Kopplung mittels Balanced Performance Indikator T2 - Tagungsband: Vernetzte Automation in funkbasierten und kabelgebundenen Netzen Y1 - 2013 SN - 978-3-7322-3553-7 PB - Books on Demand CY - Norderstedt ER -