TY - GEN A1 - Schierack, Peter A1 - Heiden, Stefan E. A1 - Khan, Muhammad Moman A1 - Nikolaus, Lena A1 - Kolenda, Rafał A1 - Stubbe, Michael A1 - Lkhagvasuren, Davaa A1 - Rödiger, Stefan A1 - Guenther, Sebastian A1 - Schaufler, Katharina T1 - Genomic and Phenotypic Analysis of an ESBL-Producing E. coli ST1159 Clonal Lineage From Wild Birds in Mongolia T2 - Frontiers in Microbiology Y1 - 2020 U6 - https://doi.org/10.3389/fmicb.2020.01699 SN - 1664-302X VL - 11 ER - TY - GEN A1 - Reimann, Ronny A1 - Rödiger, Stefan A1 - Stein, Erhard A1 - Kilian, Andrè A1 - Dittrich, Corinne A1 - Petrick, Ingolf T1 - Die Beschreibung der Fluiddynamik und der Lichtintensität im neuen MULT Reaktor KW - Photobioreaktor KW - Fluiddynamik KW - Lichtintensität Y1 - 2013 N1 - Algen und die globalen Megatrends, Tagungsband, 6. Bundesalgenstammtisch, 13. und 14. Mai 2013 Bürgerhaus Wilhelmsburg, Hamburg-Wilhelmsburg SP - S. 30 PB - DECHEMA CY - Frankfurt, M. ER - TY - GEN A1 - George, Sandra A1 - Rödiger, Stefan A1 - Schröder, Christian A1 - Knaut, Michael A1 - Küpper, Jan-Heiner T1 - Development of multiplex PCR systems for expression profiling of human cardiomyocytes induced to profilerate by lentivirus transduction of upcyte genes T2 - Journal of Cellular Biotechnology Y1 - 2016 U6 - https://doi.org/10.3233/JCB-15025 SN - 2352-3697 SN - 2352-3689 VL - 2 IS - 1 SP - 35 EP - 55 ER - TY - GEN A1 - Gruner, Melanie A1 - Moncsek, Anja A1 - Rödiger, Stefan A1 - Kühnhardt, Dagmar A1 - Feist, Eugen A1 - Stohwasser, Ralf T1 - Increased proteasome activator 28 gamma (PA28g) levels are unspecific but correlate with disease activity in rheumatoid arthritis T2 - BMC musculoskeletal disorders Y1 - 2014 U6 - https://doi.org/10.1186/1471-2474-15-414 SN - 1471-2474 VL - 15 SP - 414 EP - 424 ER - TY - GEN A1 - Choi, Youngeun A1 - Schmidt, Carsten A1 - Tinnefeld, Philip A1 - Bald, Ilko A1 - Rödiger, Stefan T1 - A new reporter design based on DNA origami nanostructures for quantification of short oligonucleotides using microbeads T2 - Scientific Reports Y1 - 2019 U6 - https://doi.org/10.1038/s41598-019-41136-x. SN - 2045-2322 IS - 9 ER - TY - GEN A1 - Whale, Alexandra S. A1 - Spiegelaere, Ward De A1 - Trypsteen, W. A1 - Nour, Afif Abdel A1 - Bae, Young-Kyung A1 - Benes, Vladimir A1 - Cleveland, Megan A1 - Devonshire, Alison S. A1 - Dong, Lianhua A1 - Drandi, Daniela A1 - Foy, Carole A. A1 - Garson, Jeremy A. A1 - He, Hua-Jun A1 - Hellemans, Jan A1 - Kubista, Mikael A1 - Lievens, Antoon A1 - Makrigiogos, Mike G. A1 - Müller, Reinhold D. A1 - Nolan, Tania A1 - O'Sullivan, Denise M. A1 - Pfaffl, Michael W. A1 - Rödiger, Stefan A1 - Romsos, Erica L. A1 - Shipley, Gregory L. A1 - Taly, Valerie A1 - Untergasser, Andreas A1 - Wittwer, Carl T. A1 - Bustin, Stephen A. A1 - Vandesompele, Jo A1 - Huggett, Jim F. T1 - The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020 T2 - Clinical Chemistry Y1 - 2020 U6 - https://doi.org/10.1093/clinchem/hvaa125 SN - 0009-9147 SN - 1530-8561 VL - 66 IS - 8 SP - 1012 EP - 1029 ER - TY - GEN A1 - Dinter, Franziska A1 - Jurischka, Christoph A1 - Weiss, Romano A1 - Rödiger, Stefan T1 - Multiparametrische Antikörperdetektion für die Diagnostik T2 - BIOspektrum Y1 - 2020 U6 - https://doi.org/10.1077/s12268-020-1375-5 SN - 1868-6249 SN - 0947-0867 VL - 26 IS - 3 SP - 265 EP - 267 ER - TY - GEN A1 - Chilimoniuk, Jarosław A1 - Gosiewska, Alicja A1 - Słowik, Jadwiga A1 - Weiss, Romano A1 - Deckert, P. Markus A1 - Rödiger, Stefan A1 - Burdukiewicz, Michał T1 - coutfitteR: efficient selection of count distributions to assess DNA damage T2 - Annals of Translational Medicine Y1 - 2021 U6 - https://doi.org/10.21037/atm-20-6363 SN - 2305-5847 VL - 9 IS - 7 ER - TY - GEN A1 - Fischer, Thomas A1 - Marchetti-Deschmann, Martina A1 - Assis, Ana Cristina A1 - Elad, Michal Levin A1 - Algarra, Manuel A1 - Barac, Marko A1 - Bogdanovic Radovic, Iva A1 - Cicconi, Flavio A1 - Claes, Britt A1 - Frascione, Nunzianda A1 - George, Sony A1 - Guedes, Alexandra A1 - Heaton, Cameron A1 - Heeren, Ron A1 - Lasic, Violeta A1 - Lerma, José Luis A1 - del Valle Martinez de Yuso Garcia, Maria A1 - Nosko, Martin A1 - O'Hara, John A1 - Oshina, Ilze A1 - Palucci, Antonio A1 - Pawlaczyk, Aleksandra A1 - Pospíšková, Kristýna A1 - de Puit, Marcel A1 - Radodic, Ksenija A1 - Rēpele, Māra A1 - Ristova, Mimoza A1 - Romolo, Francesco Saverio A1 - Šafařík, Ivo A1 - Siketic, Zdravko A1 - Spigulis, Janis A1 - Szynkowska-Jozwik, Malgorzata Iwona A1 - Tsiatsiuyeu, Andrei A1 - Vella, Joanna A1 - Dawson, Lorna A1 - Rödiger, Stefan A1 - Francese, Simona T1 - Profiling and imaging of forensic evidence – A pan-European forensic round robin study part 1: Document forgery T2 - Science & Justice Y1 - 2022 U6 - https://doi.org/10.1016/j.scijus.2022.06.001 SN - 1876-4452 SN - 1355-0306 VL - 62 IS - 4 SP - 433 EP - 447 ER - TY - CHAP A1 - Rödiger, Stefan A1 - Burdukiewicz, Michał T1 - Reproducible Analysis of DNA Amplification Experiments with an Open Source Software Environment T2 - Abstractbook, 67th Annual Meeting of the German Society of Hygiene and Microbiology (DGHM), 27.-30.09.2015, Münster Y1 - 2015 UR - https://www.dghm.org/wp-content/uploads/2018/07/783_dghm2015_abstractbook_update.pdf ER - TY - CHAP A1 - Rödiger, Stefan T1 - Applications of a Multipurpose Platf orm for the Detection and Analysis of Biomolecules T2 - BioSensor, 9. Deutsches BioSensor Symposium, 11.-13. März 2015 Y1 - 2015 PB - München ER - TY - GEN A1 - Spiess, Andrej-Nikolai A1 - Rödiger, Stefan A1 - Burdukiewicz, Michał A1 - Volksdorf, Thomas A1 - Tellinghuisen, Joel T1 - System-specific periodicity in quantitative real-time polymerase chain reaction data questions threshold-based quantitation T2 - Scientific Reports Y1 - 2016 U6 - https://doi.org/10.1038/srep38951 SN - 2045-2322 VL - 6 ER - TY - GEN A1 - Burdukiewicz, Michał A1 - Sobczyk, Piotr A1 - Rödiger, Stefan A1 - Duda-Madej, Anna A1 - Mackiewicz, Pawel A1 - Kotulska, Malgorzata T1 - Amyloidogenic motifs revealed by n-gram analysis T2 - Scientific Reports Y1 - 2017 U6 - https://doi.org/10.1038/s41598-017-13210-9 SN - 2045-2322 VL - 7 ER - TY - GEN A1 - Rödiger, Stefan A1 - Liefold, Marius A1 - Ruhe, Madeleine A1 - Reinwald, Mark A1 - Beck, Eberhard A1 - Deckert, P. Markus T1 - Quantification of DNA double-strand breaks in peripheral blood mononuclear cells from healthy donors exposed to bendamustine by an automated H2AX assay- an exploratory study T2 - Journal of Laboratory and Precision Medicine Y1 - 2018 U6 - https://doi.org/10.21037/jlpm.2018.04.10 SN - 2519-9005 VL - 3 IS - 47 ER - TY - GEN A1 - Schmidt, Carsten A1 - Rödiger, Stefan A1 - Gruner, Melanie A1 - Moncsek, Anja A1 - Stohwasser, Ralf A1 - Hanack, Katja A1 - Schierack, Peter A1 - Schröder, Christian T1 - Multiplex localization of sequential peptide epitopes by use of a planar microbead chip T2 - Analytica Chimica Acta Y1 - 2016 U6 - https://doi.org/10.1016/j.aca.2015.12.030 SN - 0003-2670 VL - 908 SP - 150 EP - 160 ER - TY - GEN A1 - Ruhe, Madeleine A1 - Rabe, Dominik A1 - Jurischka, Christoph A1 - Schröder, Julia A1 - Schierack, Peter A1 - Deckert, P. Markus A1 - Rödiger, Stefan T1 - Molecular biomarkers of DNA damage in diffuse large-cell lymphoma-a review T2 - Journal of Laboratory and Precision Medicine Y1 - 2019 U6 - https://doi.org/10.21037/jlpm.2019.01.01 SN - 2519-9005 VL - 4 ER - TY - GEN A1 - Schmidt, Jonas A1 - Berghaus, Sandro A1 - Blessing, Frithjof A1 - Wenzel, Folker A1 - Herbeck, Holger A1 - Blessing, Josef A1 - Schierack, Peter A1 - Rödiger, Stefan A1 - Roggenbuck, Dirk T1 - A semi-automated, isolation-free, high-throughput SARS-CoV-2 reverse transcriptase (RT) loop-mediated isothermal amplification (LAMP) test T2 - Scientific reports Y1 - 2021 U6 - https://doi.org/10.1038/s41598-021-00827-0 SN - 2045-2322 VL - 11 IS - 1 ER - TY - GEN A1 - Frömmel, Ulrike A1 - Lehmann, Werner A1 - Rödiger, Stefan A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Weinreich, Jörg A1 - Groß, Julia A1 - Roggenbuck, Dirk A1 - Zinke, Olaf A1 - Ansorge, Hermann A1 - Vogel, Steffen A1 - Klemm, Per A1 - Wex, Thomas A1 - Schröder, Christian A1 - Wieler, Lothar H. A1 - Schierack, Peter T1 - Adhesion of human and animal Escherichia coli strains in association with their virulence-associated genes and phylogenetic origins T2 - Applied and environmental microbiology N2 - Intestinal colonization is influenced by the ability of the bacterium to inhabit a niche, which is based on the expression of colonization factors. Escherichia coli carries a broad range of virulence-associated genes (VAGs) which contribute to intestinal (inVAGs) and extraintestinal (exVAGs) infection. Moreover, initial evidence indicates that inVAGs and exVAGs support intestinal colonization. We developed new screening tools to genotypically and phenotypically characterize E. coli isolates originating in humans, domestic pigs, and 17 wild mammal and avian species. We analyzed 317 isolates for the occurrence of 44 VAGs using a novel multiplex PCR microbead assay (MPMA) and for adhesion to four epithelial cell lines using a new adhesion assay. We correlated data for the definition of new adhesion genes. inVAGs were identified only sporadically, particularly in roe deer (Capreolus capreolus) and the European hedgehog ( Erinaceus europaeus). The prevalence of exVAGs depended on isolation from a specific host. Human uropathogenic E. coli isolates carried exVAGs with the highest prevalence, followed by badger (Meles meles) and roe deer isolates. Adhesion was found to be very diverse. Adhesion was specific to cells, host, and tissue, though it was also unspecific. Occurrence of the following VAGs was associated with a higher rate of adhesion to one or more cell lines: afa-dra, daaD, tsh, vat, ibeA, fyuA, mat, sfa-foc, malX, pic, irp2, and papC. In summary, we established new screening methods which enabled us to characterize large numbers of E. coli isolates. We defined reservoirs for potential pathogenic E. coli. We also identified a very broad range of colonization strategies and defined potential new adhesion genes. Y1 - 2013 U6 - https://doi.org/10.1128/AEM.01384-13 SN - 1098-5336 SN - 0099-2240 VL - 79 IS - 19 SP - 5814 EP - 5829 ER - TY - GEN A1 - Schierack, Peter A1 - Rödiger, Stefan A1 - Kuhl, Christoph A1 - Hiemann, Rico A1 - Roggenbuck, Dirk A1 - Li, Ganwu A1 - Weinreich, Jörg A1 - Berger, Enrico A1 - Nolan, Lisa K. A1 - Nicholson, Bryon A1 - Römer, Antje A1 - Frömmel, Ulrike A1 - Wieler, Lothar H. A1 - Schröder, Christian T1 - Porcine E. coli: Virulence-Associated Genes, Resistance Genes and Adhesion and Probiotic Activity Tested by a New Screening Method T2 - PLoS One N2 - We established an automated screening method to characterize adhesion of Escherichia coli to intestinal porcine epithelial cells (IPEC-J2) and their probiotic activity against infection by enteropathogenic E. coli (EPEC). 104 intestinal E. coli isolates from domestic pigs were tested by PCR for the occurrence of virulence-associated genes, genes coding for resistances to antimicrobial agents and metals, and for phylogenetic origin by PCR. Adhesion rates and probiotic activity were examined for correlation with the presence of these genes. Finally, data were compared with those from 93 E. coli isolates from wild boars. Isolates from domestic pigs carried a broad variety of all tested genes and showed great diversity in gene patterns. Adhesions varied with a maximum of 18.3 or 24.2 mean bacteria adherence per epithelial cell after 2 or 6 hours respectively. Most isolates from domestic pigs and wild boars showed low adherence, with no correlation between adhesion/probiotic activity and E. coli genes or gene clusters. The gene sfa/foc, encoding for a subunit of F1C fimbriae did show a positive correlative association with adherence and probiotic activity; however E. coli isolates from wild boars with the sfa/foc gene showed less adhesion and probiotic activity than E. coli with the sfa/foc gene isolated from domestic pigs after 6 hour incubation. In conclusion, screening porcine E. coli for virulence associated genes genes, adhesion to intestinal epithelial cells, and probiotic activity revealed a single important adhesion factor, several probiotic candidates, and showed important differences between E. coli of domestic pigs and wild boars. Y1 - 2013 U6 - https://doi.org/10.1371/journal.pone.0059242 VL - 8 IS - 4 ER - TY - GEN A1 - Rödiger, Stefan A1 - Lehmann, Werner A1 - Frömmel, Ulrike A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Burdukiewicz, Michał A1 - Schröder, Christian A1 - Dangla, Rémi A1 - Droniou, Magali A1 - Schierack, Peter T1 - The Modi Operandi of the VideoScan Platform for the Detection and Analysis of Nucleic Acids. Life Science Day 2013 & 17. Leibniz Conference of Advanced Science, Freie Universität Berlin, 24.10.2013 Y1 - 2013 UR - https://leibniz-institut.de/Konferenzen/lsd2013/roediger_the_modi_operandi_of_the_videoscan_platform.pdf ER - TY - GEN A1 - Schierack, Peter A1 - Rödiger, Stefan A1 - Kolenda, Rafał A1 - Hiemann, Rico A1 - Berger, Enrico A1 - Grzymajlo, Krzysztof A1 - Swidsinski, Alexander A1 - Juretzek, Thomas A1 - Meissner, Dirk A1 - Mydlak, Karsten A1 - Reinhold, Dirk A1 - Nolan, Lisa K. A1 - Roggenbuck, Dirk T1 - Species-specific and pathotype-specific binding of bacteria to zymogen granule membrane glycoprotein 2 (GP2) T2 - Gut Y1 - 2015 U6 - https://doi.org/10.1136/gutjnl-2014-307854 SN - 1468-3288 SN - 0017-5749 VL - 64 IS - 3 SP - 517 EP - 519 ER - TY - GEN A1 - Schneider, Jens A1 - Weiss, Romano A1 - Ruhe, Madeleine A1 - Jung, Tobias A1 - Roggenbuck, Dirk A1 - Stohwasser, Ralf A1 - Schierack, Peter A1 - Rödiger, Stefan T1 - Open source bioimage informatic tools for the analysis of DNA damage and associated biomarkers T2 - Journal of Laboratory and Precision Medicine KW - bioimage informatics KW - DNA damage response (DDR) KW - double-strand break (DSB) KW - immunofluorescence microscopy KW - open source software KW - proteasome activator PA28gamma Y1 - 2019 U6 - https://doi.org/10.21037/jlpm.2019.04.05 SN - 2519-9005 VL - 4 ER - TY - GEN A1 - Scholz, Juliane A1 - Großmann, Kai A1 - Knütter, Ilka A1 - Hiemann, Rico A1 - Sowa, Mandy A1 - Röber, Nadja A1 - Rödiger, Stefan A1 - Schierack, Peter A1 - Reinhold, Dirk A1 - Bogdanos, Dimitrios Petrou A1 - Meroni, Pier Luigi A1 - Radice, Antonella A1 - Conrad, Karsten A1 - Roggenbuck, Dirk T1 - Second generation analysis of antinuclear antibody (ANA) by combination of screening and confirmatory testing T2 - Clinical Chemistry and Laboratory Medicine Y1 - 2015 U6 - https://doi.org/10.1515/cclm-2015-0083 SN - 1437-4331 VL - 53 IS - 12 SP - 1991 EP - 2002 ER - TY - GEN A1 - Olowe, Olugbenga Adekunle A1 - Adefioye, Olusolabomi J. A1 - Ajayeoba, T. A. A1 - Schiebel, Juliane A1 - Weinreich, Jörg A1 - Ali, Aamir A1 - Burdukiewicz, Michał A1 - Rödiger, Stefan A1 - Schierack, Peter T1 - Phylogenetic grouping and biofilm formation of Multidrug Resistant Escherichia coli Isolates from Humans, Animals and Food products in South-west Nigeria T2 - Scientific African Y1 - 2019 U6 - https://doi.org/10.1016/j.sciaf.2019.e00158 SN - 2468-2276 VL - 6 ER - TY - GEN A1 - Liebsch, Claudia A1 - Rödiger, Stefan A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Weinreich, Jörg A1 - Fruth, Angelika A1 - Roggenbuck, Dirk A1 - Lehmann, Werner A1 - Schedler, Uwe A1 - Juretzek, Thomas A1 - Schierack, Peter T1 - Solid-phase microbead array for multiplex O-serotyping of Escherichia coli T2 - Microchimica Acta Y1 - 2017 U6 - https://doi.org/10.1007/s00604-017-2088-4 SN - 0026-3672 SN - 1436-5073 VL - 184 IS - 5 SP - 1405 EP - 1415 ER - TY - GEN A1 - Rödiger, Stefan A1 - Liebsch, Claudia A1 - Schmidt, Carsten A1 - Lehmann, Werner A1 - Resch-Genger, U. A1 - Schedler, Uwe A1 - Schierack, Peter T1 - Nucleic acid detection based on the use of microbeads: a review T2 - Microchimica Acta Y1 - 2014 U6 - https://doi.org/10.1007/s00604-014-1243-4 SN - 0026-3672 SN - 1436-5073 VL - 181 IS - 11-12 SP - 1151 EP - 1168 ER - TY - CHAP A1 - Rödiger, Stefan A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Stavitskaya, Luba A1 - Gruner, Melanie A1 - Kundzer, Alena V. A1 - Volkova, Margarita V. A1 - Generalov, I. A1 - Schmidt, Carsten A1 - Schröder, Christian A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Development of a Method for Multiplex Real-Time Analysis of Enzymatic Activity on a Microbead-Chip T2 - Infection, tumors and autoimmunity, report on the 11th Dresden Symposium on Autoantibodies held in Dresden on September 1-4, 2013 Y1 - 2013 SN - 978-3-89967-881-9 SP - 275 EP - 280 PB - Pabst Science Publ. CY - Lengerich [u.a.] ER - TY - GEN A1 - Khan, Muhammad Moman A1 - Kolenda, Rafał A1 - Schierack, Peter A1 - Weinreich, Jörg A1 - Rödiger, Stefan A1 - Schierack, Jakob A1 - Stubbe, Michael A1 - Lkhagvasuren, Davaa A1 - Guenther, Sebastian A1 - Schaufler, Katharina T1 - Investigation of Commensal Escherichia coli Populations of Cormorant Hatchlings in the Absence of Anthropogenic Impacts in Remote Areas of West Mongolia T2 - Microorganisms Y1 - 2021 U6 - https://doi.org/10.3390/microorganisms9020372 SN - 2076-2607 VL - 9 IS - 2 ER - TY - GEN A1 - Schmidt, Carsten A1 - Borcherding, Heike A1 - Thiele, Thomas A1 - Schedler, Uwe A1 - Werner, Franziska A1 - Rödiger, Stefan A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Fluorescence-encoded poly (methyl metharcylate) nanoparticles for a lateral flow assay detecting IgM autoantibodies in rheumatoid arthritis T2 - Analytical biochemistry Y1 - 2021 U6 - https://doi.org/10.1016/journal.ppat.1010118 SN - 1096-0309 VL - Vol. 633 ER - TY - GEN A1 - Schmidt, Carsten A1 - Kammel, Anne A1 - Tanner, Julian A. A1 - Kinghorn, Andrew B. A1 - Khan, Muhammad Moman A1 - Lehmann, Werner A1 - Menger, Marcus A1 - Schedler, Uwe A1 - Schierack, Peter A1 - Rödiger, Stefan T1 - A Multiparametic Fluorescence Assay for Screening Aptamer-Protein Interactions Based on Microbeads T2 - Scientific Reports Y1 - 2022 U6 - https://doi.org/10.1038/s41598-022-06817-0 SN - 2045-2322 VL - 12 ER - TY - GEN A1 - Hanschmann, Henning A1 - Rödiger, Stefan A1 - Kramer, Toni A1 - Hanschmann, Katrin A1 - Steidle, Michael A1 - Fingerle, Volker A1 - Schmidt, Carsten A1 - Lehmann, Werner A1 - Schierack, Peter T1 - LoopTag FRET Probe System for Multiplex qPCR Detection of Borrelia Species T2 - Life Y1 - 2021 U6 - https://doi.org/10.3390/life11111163 SN - 2075-1729 VL - 11 IS - 11 ER - TY - GEN A1 - Sowa, Mandy A1 - Murugaiyan, Jayaseelan A1 - Conrad, Karsten A1 - Laass, Martin W. A1 - Bogdanos, Dimitrios Petrou A1 - Papp, Maria A1 - Rödiger, Stefan A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - A novel neutrophil autoantigenic target in inflammatory bowel disease Y1 - 2018 UR - https://www.researchgate.net/publication/335889899_A_novel_neutrophil_autoantigenic_target_in_inflammatory_bowel_disease ER - TY - GEN A1 - Rödiger, Stefan A1 - Schneider, Jens A1 - Sauer, Lysann A1 - Reddig, Annika A1 - Schierack, Peter A1 - Roggenbuck, Dirk A1 - Schröder, Christian T1 - A Multiparameter Platform for The Analysis of DNA Double-strand Breaks and Associated Biomarkers after genome Editing Austrian Biomarker Symposium (ASBD) 2016, 10.-11.03.2016, Wien, Österreich Y1 - 2016 UR - https://f1000research.com/posters/5-350 ER - TY - GEN A1 - Burdukiewicz, Michał A1 - Spiess, Andrej-Nikolai A1 - Blagodatskikh, Konstantin A. A1 - Lehmann, Werner A1 - Schierack, Peter A1 - Rödiger, Stefan T1 - Algorithms for automated detection of hook effect-bearing amplification curves T2 - Biomolecular Detection and Quantification Y1 - 2018 U6 - https://doi.org/10.1016/j.bdq.2018.08.001 SN - 2214-7535 VL - 16 SP - 1 EP - 4 ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Rödiger, Stefan A1 - Schierack, Peter A1 - Roggenbuck, Dirk T1 - Preface to the Special Issue on PCR on chip and related technologies T2 - Microchimica Acta Y1 - 2014 U6 - https://doi.org/10.1007/s00604-014-1246-1 VL - 181 IS - 13/14 SP - 1609 EP - 1610 ER - TY - GEN A1 - Kolenda, Rafał A1 - Burdukiewicz, Michał A1 - Schiebel, Juliane A1 - Rödiger, Stefan A1 - Sauer, Lysann A1 - Szabo, Istvan A1 - Orlowska, Aleksandra A1 - Weinreich, Jörg A1 - Nitschke, Jörg A1 - Böhm, Alexander A1 - Gerber, Ulrike A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Adhesion of Salmonella to Pancreatic Secretory Granule Membrane Major Glycoprotein GP2 of Human and Porcine Origin Depends on FimH Sequence Variation T2 - Frontiers in Microbiology Y1 - 2018 U6 - https://doi.org/10.3389/fmicb.2018.01905 SN - 1664-302X VL - 9 IS - 1905 ER - TY - GEN A1 - Bartlitz, Christin A1 - Kolenda, Rafał A1 - Chilimoniuk, Jarosław A1 - Grzymajlo, Krzysztof A1 - Rödiger, Stefan A1 - Bauerfeind, Rolf A1 - Ali, Aamir A1 - Tchesnokovag, Veronika A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Adhesion of Enteropathogenic, Enterotoxigenic, and Commensal Escherichia coli to the Major Zymogen Granule Membrane Glyoprotein 2 T2 - Applied abd Environmental Microbiology Y1 - 2022 U6 - https://doi.org/10.1128/aem.02279-21 SN - 1098-5536 VL - 88 IS - 5 ER - TY - GEN A1 - Großmann, Kai A1 - Röber, Nadja A1 - Hiemann, Rico A1 - Rödiger, Stefan A1 - Schierack, Peter A1 - Reinhold, Dirk A1 - Laass, Martin W. A1 - Conrad, Karsten A1 - Roggenbuck, Dirk T1 - Simultaneous detection of celiac disease-specific IgA antibodies and total IgA T2 - Autoimmunity Highlights Y1 - 2016 U6 - https://doi.org/10.1007/s13317-016-0073-2 SN - 2038-0305 SN - 2038-3274 VL - 7 IS - 2 ER - TY - CHAP A1 - Rödiger, Stefan A1 - Schierack, Peter A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Berger, Ingo A1 - Frömmel, Ulrike A1 - Schmidt, Carsten A1 - Ruhland, Mirko A1 - Schimke, Ingolf A1 - Roggenbuck, Dirk A1 - Lehmann, Werner A1 - Schröder, Christian ED - Seitz, Harald ED - Berger, Ingo T1 - A highly versatile microscope imaging technology platform for the multiplex real-time detection of biomolecules and autoimmune antibodies T2 - Molecular diagnostics Y1 - 2013 SN - 978-3-642-37690-0 SN - 978-3-642-37691-7 SP - 35 EP - 74 PB - Springer CY - Berlin [u.a.] ER - TY - GEN A1 - Kramer, Toni A1 - Schiebel, Juliane A1 - Zelck, Claudia A1 - Weinreich, Jörg A1 - Schierack, Peter A1 - Rödiger, Stefan T1 - Genotyping and gene expression analysis of putative reference genes in Enterobacteriaceae, 67th Annual Meeting of the German Society of Hygiene and Microbiology (DGHM), 27.-30.09.2015, Münster Y1 - 2015 UR - https://f1000research.com/posters/5-248 U6 - https://doi.org/10.7490/f1000research.1111354.1 ER - TY - GEN A1 - Dinter, Franziska A1 - Burdukiewicz, Michał A1 - Schierack, Peter A1 - Lehmann, Werner A1 - Nestler, Jörg A1 - Dame, Gregory A1 - Rödiger, Stefan T1 - Simultaneous detection and quantification of DNA and protein biomarkers in spectrum of cardiovascular diseases in a microfluidic microbead chip T2 - Analytical and Bioanalytical Chemistry Y1 - 2019 U6 - https://doi.org/10.1007/s00216-019-02199-x SN - 1618-2650 SN - 1618-2642 VL - 411 IS - 29 SP - 7725 EP - 7735 ER - TY - GEN A1 - Schiebel, Juliane A1 - Noack, Jonas A1 - Rödiger, Stefan A1 - Kammel, Anne A1 - Menzel, Friederike A1 - Schwibbert, Karin A1 - Weise, Matthias A1 - Weiss, Romano A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Elimport, Alexey A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Analysis of three-dimensional biofilms on different material surfaces T2 - Biomaterials Science Y1 - 2020 U6 - https://doi.org/10.1039/D0BM00455C SN - 2047-4849 VL - 8 IS - 12 SP - 3500 EP - 3510 ER - TY - GEN A1 - Burdukiewicz, Michał A1 - Rödiger, Stefan A1 - Sobczyk, Piotr A1 - Menschikowski, Mario A1 - Schierack, Peter A1 - Mackiewicz, Pawel T1 - Methods for comparing multiple digital PCR experiments T2 - Biomolecular Detection and Quantification Y1 - 2016 U6 - https://doi.org/10.1016/j.bdq.2016.06.004 SN - 2214-7535 VL - 9 SP - 14 EP - 19 ER - TY - GEN A1 - Jurischka, Christoph A1 - Dinter, Franziska A1 - Efimova, Anastasia A1 - Weiss, Romano A1 - Schiebel, Juliane A1 - Schulz, Christian A1 - Fayziev, Bekzodjon A1 - Schierack, Peter A1 - Fischer, Thomas A1 - Rödiger, Stefan T1 - An explorative study of polymers for 3D printing of bioanalytical test systems T2 - Clinical Hemorheology and Microcirculation N2 - Background: The 3D printing is relevant as a manufacturing technology of functional models for forensic, pharmaceutical and bioanalytical applications such as drug delivery systems, sample preparation and point-of-care tests. Objective: Melting behavior and autofluorescence of materials are decisive for optimal printing and applicability of the product which are influenced by varying unknown additives. Methods: We have produced devices for bioanalytical applications from commercially available thermoplastic polymers using a melt-layer process. We characterized them by differential scanning calorimetry, fluorescence spectroscopy and functional assays (DNA capture assay, model for cell adhesion, bacterial adhesion and biofilm formation test). Results: From 14 tested colored, transparent and black materials we found only deep black acrylonitrile-butadiene-styrene (ABS) and some black polylactic acid (PLA) useable for fluorescence-based assays, with low autofluorescence only in the short-wave range of 300-400 nm. PLA was suitable for standard bioanalytical purposes due to a glass transition temperature of approximately 60°C, resistance to common laboratory chemicals and easy print processing. For temperature-critical methods, such as hybridization reactions up to 90°C, ABS was better suited. Conclusions: Autofluorescence was not a disadvantage per se but can also be used as a reference signal in assays. The rapid development of individual protocols for sample processing and analysis required the availability of a material with consistent quality over time. For fluorescence-based assays, the use of commercial standard materials did not seem to meet this requirement. KW - Thermal analysis KW - 3D Printing Y1 - 2020 UR - https://pubmed.ncbi.nlm.nih.gov/31929149/ U6 - https://doi.org/10.3233/CH-190713 SN - 1875-8622 VL - 75 IS - 1 SP - 57 EP - 84 ER - TY - GEN A1 - Schiebel, Juliane A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Burdukiewicz, Michał A1 - Weinreich, Jörg A1 - Ali, Aamir A1 - Roggenbuck, Dirk A1 - Rödiger, Stefan A1 - Schierack, Peter T1 - Genotypic and phenotypic characteristics in association with biofilm formation in different pathotypes of human clinical Escherichia coli isolates T2 - Applied and Environmental Microbiology Y1 - 2017 U6 - https://doi.org/10.1128/AEM.01660-17 SN - 1098-5336 SN - 0099-2240 VL - 83 IS - 24 ER - TY - GEN A1 - Liedtke, Victoria A1 - Schröder, Christian A1 - Roggenbuck, Dirk A1 - Weiss, Romano A1 - Stohwasser, Ralf A1 - Schierack, Peter A1 - Rödiger, Stefan A1 - Schenk, Lysann T1 - LEDGF/p75 is required for an efficient DNA damage response T2 - International Journal of Molecular Sciences KW - LEDGF KW - CRISPR/Cas9 KW - DNA damage signaling KW - gH2AX KW - ubiquitination Y1 - 2021 U6 - https://doi.org/10.3390/ijms22115866 VL - 22 IS - 11 SP - 1 EP - 16 ER - TY - GEN A1 - Rödiger, Stefan A1 - Ruhland, Mirko A1 - Schröder, Christian A1 - Großmann, Kai A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Berger, Ingo A1 - Schimke, Ingolf A1 - Schierack, Peter T1 - Fluorescence dye adsorption assay to quantify carboxyl groups on the surface of poly(methyl methacrylate) microbeads T2 - Analytical Chemistry Y1 - 2011 U6 - https://doi.org/10.1021/ac103277s SN - 0003-2700 SN - 1520-6882 VL - 83 IS - 9 SP - 3379 EP - 3385 ER - TY - GEN A1 - Deutschmann, Claudia A1 - Dinter, Franziska A1 - Rödiger, Stefan A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Comparison of Lab and Point of Care (POC) technologies – case study for CHI3L1, Potsdam Days on Bioanalysis Y1 - 2017 UR - https://www.researchgate.net/publication/321419307 ER - TY - CHAP A1 - Rödiger, Stefan A1 - Hanschmann, Henning A1 - Steidle, Michael A1 - Schröder, Christian A1 - Schierack, Peter A1 - Lehmann, Werner T1 - Detection of Borrelia, Bordetella and HPV with the LoopTag Real-Time PCR Probe System T2 - International journal of medical microbiology Y1 - 2013 SN - 1618-0607 SN - 1438-4221 N1 - 65. Jahrestagung der Deutschen Gesellschaft für Hygiene und Mikrobiologie e.V. und der Jahrestagung der Deutschen Gesellschaft für Infektiologie (DGI) e.V., Universität Rostock, 22.09. – 25.09.20 13, Rostock VL - 303 IS - Supplement 1 SP - 12 EP - 13 ER - TY - GEN A1 - Frömmel, Ulrike A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Weinreich, Jörg A1 - Groß, Julia A1 - Rödiger, Stefan A1 - Wex, Thomas A1 - Ansorge, Hermann A1 - Zinke, Olaf A1 - Schröder, Christian A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Adhesion patterns of commensal and pathogenic Escherichia coli from humans and wild animals on human and porcine epithelial cell lines T2 - Gut Pathogens N2 - Abstract BACKGROUND: Different strategies of colonization or infection by E. coli result in formation of certain adhesion patterns which help also in classifying intestinal E. coli into pathotypes. Little is known about adhesion patterns and host- and tissue adaption of commensal E. coli and about E. coli originating in clinically healthy hosts carrying pathotype-specific virulence-associated genes. FINDINGS: Adhesion pattern of E. coli (n = 282) from humans and from 18 animal species were verified on intestinal human Caco-2 and porcine IPEC-J2 cells and, furthermore, for comparison on human urinary bladder 5637, porcine kidney PK-15 epithelial and HEp-2 cells. The analysis was carried out on 150,000 images of adhesion assays.Adhesion patterns were very diverse; 88 isolates were completely non-adherent, whereas 194 adhered to at least one cell line with the dominant adhesion patterns "diffusely distributed" and "microcolony formation". Adhesion patterns "chains" and "clumps" were also visible. Chain formation was mediated by the presence of epithelial cells. Clump formation was very specific on only the 5637 cell line. All enteropathogenic (eae+) E. coli (EPEC; n = 14) were able to form microcolonies which was cell line specific for each isolate. Most EPEC formed microcolonies on intestinal IPEC-J2 and Caco-2 but several also on urinary tract cells. Shigatoxin-producing (stx+) E. coli (n = 10) showed no specific adhesion patterns. CONCLUSIONS: E. coli isolates were highly diverse. Commensal and pathogenic isolates can adhere in various forms, including diffuse distribution, microcolonies, chains and clumps. Microcolony formation seems to be a global adhesion strategy also for commensal E. coli. Y1 - 2013 U6 - https://doi.org/10.1186/1757-4749-5-31 SN - 1757-4749 VL - 5 IS - 31 ER - TY - GEN A1 - Reddig, Annika A1 - Rübe, Claudia E. A1 - Rödiger, Stefan A1 - Schierack, Peter A1 - Reinhold, Dirk A1 - Roggenbuck, Dirk T1 - DNA damage assessment and potential applications in laboratory diagnostics and precision medicine T2 - Journal of Laboratory and Precision Medicine Y1 - 2018 U6 - https://doi.org/10.21037/jlpm.2018.03.06 SN - 2519-9005 VL - 3 ER - TY - GEN A1 - Deutschmann, Claudia A1 - Sowa, Mandy A1 - Murugaiyan, Jayaseelan A1 - Roessler, Uwe A1 - Röber, Nadja A1 - Conrad, Karsten A1 - Laass, Martin W. A1 - Bogdanos, Dimitrios Petrou A1 - Sipeki, Nora A1 - Papp, Maria A1 - Rödiger, Stefan A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Identification of Chitinase-3-Like Protein 1 as a Novel Neutrophil Antigenic Target in Crohn’s Disease T2 - Journal of Crohn's and Colitis N2 - Background and Aims There is an increasing incidence of inflammatory bowel disease [IBD]. Autoimmune responses are involved in the pathophysiology of IBD, but their underlying pathways and target antigens have not yet been fully elucidated. Methods Autoantigenic targets in IBD were identified after separation of whole cell proteins isolated from neutrophils using two-dimensional electrophoresis and matrix assisted laser desorption ionization – time of flight mass spectrometry-based protein identification of the spots that displayed Western blotting signals with anti-neutrophil cytoplasmic antibody-positive sera. The prevalence of IgG, IgA and secretory IgA [sIgA] to chitinase 3-like protein 1 [CHI3L1] was analysed by enzyme-linked immunosorbent assays using recombinant CHI3L1 in 110 patients with Crohn’s disease [CD], 95 with ulcerative colitis [UC], 126 with coeliac disease [CeD] and 86 healthy controls [HCs]. Results The 18-glycosylhydrolase family member CHI3L1 was identified as a neutrophil autoantigenic target. CD patients displayed significantly higher levels of IgG to CHI3L1 than patients with UC and CeD (p < 0.0001, respectively). IgA and sIgA to CHI3L1 was significantly higher in CD than in UC, CeD and HCs [p < 0.0001, respectively]. IgA and sIgA to CHI3L1 demonstrated the highest prevalence in CD [25.5%, 28/110; and 41.8%%, 46/110] compared to HCs [2.3%, 2/86; and 4.7%%, 4/86; p = 0.0015 and p < 0.0001] and are associated with a more complicated progression of CD. Conclusion CHI3L1 is a novel neutrophil autoantigenic target in CD. IgA and sIgA to CHI3L1 may serve as novel markers for CD and may facilitate the serological diagnosis of IBD. Y1 - 2019 U6 - https://doi.org/10.1093/ecco-jcc/jjz012 SN - 1876-4479 SN - 1873-9946 VL - 13 IS - 7 SP - 894 EP - 904 ER - TY - GEN A1 - Kundzer, Alena V. A1 - Volkova, Margarita V. A1 - Bogdanos, Dimitrios Petrou A1 - Rödiger, Stefan A1 - Schierack, Peter A1 - Generalov, I. A1 - Nevinsky, G. A. A1 - Roggenbuck, Dirk T1 - Deoxyribonuclease activity of polyclonal IgGs: a putative serological marker in patients with spondyloarthritides T2 - Immunologic Research N2 - Antibodies executing catalytic activity are referred to as antibody enzymes or short “abzymes” and may have diagnostic relevance. Abzymes with deoxyribonuclease (DNase) activity have been demonstrated in patients with autoimmune and infectious diseases. Despite several reports on the occurrence of DNase abzymes in systemic autoimmune rheumatic diseases, conclusive data about DNase activity of antibodies in patients with spondyloarthritides (SpAs) are lacking. In recent cross-sectional studies evaluating levels of IgG DNase activity in patients with psoriatic arthritis (PsA), reactive arthritis (ReA), and ankylosing spondylitis (AS), DNase activity of IgG has been assessed by the rivanol clot method and confirmed by agarose gel electrophoresis. Remarkably, levels of IgG DNase activity were significantly higher in sera of SpA patients than those in control subjects. In patients with PsA, ReA, and AS, a positive correlation of DNase IgG activity with synovitis, disease activity, and stage of spondylitis was observed, respectively. Given the involvement of autoimmune reactions in cytolysis and connective tissue degradation in PsA, ReA, and to a lesser extent in AS, abzymes might have an impact on the pathophysiology of SpAs. Detection of IgG DNase activity in patients suffering from SpA represents an exciting new research field and may assist in the differential diagnosis of SpA. Y1 - 2013 U6 - https://doi.org/10.1007/s12026-013-8424-9 SN - 0257-277X SN - 1559-0755 VL - 56 IS - 2-3 SP - 457 EP - 464 ER - TY - GEN A1 - Willitzki, Annika A1 - Hiemann, Rico A1 - Peters, Vanessa A1 - Sack, Ulrich A1 - Schierack, Peter A1 - Rödiger, Stefan A1 - Anderer, Ursula A1 - Conrad, Karsten A1 - Bogdanos, Dimitrios Petrou A1 - Reinhold, Dirk A1 - Roggenbuck, Dirk T1 - New PlatformTechnology for Comprehensive Serological Diagnostics of Autoimmune Diseases T2 - Clinical and Developmental Immunology KW - systemic autoimmune rheumatic disease (SARD) KW - automated IIF methods KW - platform for automated interpretation of cell-based IIF testing KW - microbead immunoassays KW - multiplexing Y1 - 2012 U6 - https://doi.org/10.1155/2012/284740 SN - 1740-2530 SN - 1740-2522 SP - 1 EP - 8 ER - TY - GEN A1 - Sowa, Mandy A1 - Großmann, Kai A1 - Scholz, Juliane A1 - Röber, Nadja A1 - Rödiger, Stefan A1 - Schierack, Peter A1 - Conrad, Karsten A1 - Roggenbuck, Dirk A1 - Hiemann, Rico T1 - Der CytoBead-Assay – Eine neue Möglichkeit der multiparametrischen Autoantikörperanalytik bei systemischen Autoimmunerkrankungen T2 - Journal of Laboratory Medicine Y1 - 2014 U6 - https://doi.org/10.1515/labmed-2014-0041 VL - 38 IS - 6 SP - 309 EP - 317 ER - TY - GEN A1 - Deutschmann, Claudia A1 - Roggenbuck, Dirk A1 - Schierack, Peter A1 - Rödiger, Stefan T1 - Autoantibody testing by enzyme-linked immunosorbent assay-a case in which the solid phase decides on success and failure T2 - Heliyon Y1 - 2020 U6 - https://doi.org/10.1016/j.heliyon.2020.e03270 SN - 2405-8440 VL - 6 IS - 1 ER - TY - GEN A1 - Dinter, Franziska A1 - Deutschmann, Claudia A1 - Schierack, Peter A1 - Dame, Gregory A1 - Rödiger, Stefan T1 - Integration of Cardiovascular Disease Biomarkers in a Microfluidic Microbead Chip Y1 - 2017 UR - https://f1000research.com/posters/6-2136 N1 - Potsdam Days on Bioanalysis 2017 ER - TY - GEN A1 - Schmidt, Jonas A1 - Berghaus, Sandro A1 - Blessing, Frithjof A1 - Wenzel, Folker A1 - Herbeck, Holger A1 - Blessing, Josef A1 - Schierack, Peter A1 - Rödiger, Stefan A1 - Roggenbuck, Dirk T1 - Serological and viral genetic features of patients with COVID-19 in a selected German patient cohort-correlation with disease characteristics T2 - GeroScience Y1 - 2021 U6 - https://doi.org/10.1007/s11357-021-00443-w SN - 2509-2723 SN - 2509-2715 VL - 43 IS - 5 SP - 2249 EP - 2264 ER - TY - GEN A1 - Rödiger, Stefan A1 - Kramer, Toni A1 - Frömmel, Ulrike A1 - Weinreich, Jörg A1 - Roggenbuck, Dirk A1 - Guenther, Sebastian A1 - Schaufler, Katharina A1 - Schröder, Christian A1 - Schierack, Peter T1 - Intestinal Escherichia coli colonization in a mallard duck population over four consecutive winter season T2 - Environmental Microbiology Y1 - 2015 U6 - https://doi.org/10.1111/1462-2920.12807 SN - 1462-2920 SN - 1462-2912 VL - 17 IS - 9 SP - 3352 EP - 3361 ER - TY - GEN A1 - Rödiger, Stefan A1 - Burdukiewicz, Michał A1 - Blagodatskikh, Konstantin A. A1 - Jahn, Michael A1 - Schierack, Peter T1 - R as an Environment for the Reproducible Analysis of DNA Amplification Experiments T2 - The R journal Y1 - 2015 UR - https://journal.r-project.org/archive/2015/RJ-2015-011/index.html SN - 2073-4859 VL - 7 IS - 1 SP - 127 EP - 150 ER - TY - GEN A1 - Sauer, Lysann A1 - Rödiger, Stefan A1 - Schneider, Jens A1 - Schierack, Peter A1 - Roggenbuck, Dirk A1 - Schröder, Christian T1 - Functional Multiparameter Analy sis of Double-Strand Breaks and Associate Biomarkers during Genome Editing, Genome Editing and Gene Modulation Congress 2016, 6.–8.04.2016, Oxford, United Kingdom Y1 - 2016 UR - https://f1000research.com/posters/5-706 ER - TY - CHAP A1 - Rödiger, Stefan A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Stavitskaya, Luba A1 - Gruner, Melanie A1 - Kundzer, Alena V. A1 - Volkova, Margarita V. A1 - Generalov, I. A1 - Schmidt, Carsten A1 - Schröder, Christian A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Development of a Method for Multiplex Real-Time Analysis of Enzymati c Activity on a Microbead-Chip. T2 - Infections, Tumors and Autoimmunity, Report on the 11th Dresden Symposium on Autoantibodies held in Dresden on September 01. - 04., 2013 Y1 - 2013 SN - 978-3-89967-881-9 SP - 280 PB - Pabst Science Publ. CY - Lengerich ER - TY - CHAP A1 - Rödiger, Stefan A1 - Burdukiewicz, Michał A1 - Heiserich, Lisa A1 - Schierack, Peter A1 - Roggenbuck, Dirk T1 - Digital Enumeration of Double Strand Breaks via γH2AX and Aassociated biomarkers in a Computing Environment for Reproducible Research T2 - From autoantibody research to standardized diagnostic assays in the management of human diseases, report on the 12th Dresden Symposium on Autoantibodies, September 23-26, 2015 Y1 - 2015 SN - 978-3-95853-104-8 SP - S. 413 PB - Pabst Science Publishers CY - Lengerich ER - TY - GEN A1 - Liedtke, Victoria A1 - Rose, Laura A1 - Hiemann, Rico A1 - Nasser, Abdullah A1 - Rödiger, Stefan A1 - Bonaventura, Alena A1 - Winkler, Laura A1 - Sowa, Mandy A1 - Stöckle, Michael A1 - Schierack, Peter A1 - Junker, Kerstin A1 - Roggenbuck, Dirk T1 - Over-Expression of LEDGF/p75 in HEp-2 Cells Enhances Autoimmune IgG Response in Patients with Benign Prostatic Hyperplasia—A Novel Diagnostic Approach with Therapeutic Consequence? T2 - International Journal of Molecular Sciences N2 - Lens epithelium-derived growth factor splice variant of 75 kDa (LEDGF/p75) is an autoantigen over-expressed in solid tumors and acts as a stress-related transcriptional co-activator. Participation of autoimmune responses in the pathophysiology of benign prostatic hyperplasia (PBH) and a corresponding immunosuppressive therapy by TNFalpha antagonists has been recently suggested. Thus, autoAb testing could aid in the diagnosis of BPH patients profiting from such therapy. We generated CRISPR/Cas9 modified HEp-2 LEDGF knock-out (KO) and HEp-2 LEDGF/p75 over-expressing (OE) cells and examined IgG autoantibody reactivity to LEDGF/p75 in patients with prostate cancer (PCa, n = 89), bladder cancer (BCa, n = 116), benign prostatic hyperplasia (BPH, n = 103), and blood donors (BD, n = 60) by indirect immunofluorescence assay (IFA). Surprisingly, we could not detect elevated binding of autoAbs against LEDGF/p75 in cancer patients, but autoAb reactivity to LEDGF/p75 OE cells in about 50% of patients with BPH was unexpectedly significantly increased. Furthermore, a line immunoassay enabling the detection of 18 different autoAbs revealed a significantly increased occurrence of anti-dsDNA autoAbs in 34% of BPH patients in contrast to tumor patients and BD. This finding was confirmed by anti-mitochondrial (mDNA) autoAb detection with the Crithidia luciliae immunofluorescence test, which also showed a significantly higher prevalence (34%) of anti-mDNA autoAbs in BPH. In summary, our study provided further evidence for the occurrence of autoimmune responses in BPH. Furthermore, LEDGF/p75 over-expression renders HEp-2 cells more autoantigenic and an ideal target for autoAb analysis in BPH with a potential therapy consequence. KW - LEDGF/p75 KW - autoimmunity KW - CRISPR/Cas9 KW - dsDNA KW - mDNA Y1 - 2023 U6 - https://doi.org/10.3390/ijms24076166 SN - 1422-0067 VL - 24 IS - 7 ER - TY - GEN A1 - Lopens, Steffi A1 - Wunsch, Ewa A1 - Milkiewicz, Malgorzata A1 - Röber, Nadja A1 - Zarske, Grit A1 - Nasser, Abdullah A1 - Conrad, Karsten A1 - Laass, Martin W. A1 - Rödiger, Stefan A1 - Krawczyk, Marcin A1 - Roggenbuck, Dirk A1 - Milkiewicz, Piotr T1 - PR3-ANCAs Detected by Third-Generation ELISA Predicts Severe Disease and Poor Survival in Primary Sclerosing Cholangitis T2 - Diagnostics N2 - A highly sensitive detection of anti-neutrophil cytoplasmic antibodies to serine proteinase-3 (PR3-ANCAs) aids in the serological diagnosis of autoimmune liver disorders and the prediction of severity in primary sclerosing cholangitis (PSC). Here, we evaluate a novel third-generation ELISA for the detection of PR3-ANCAs. In total, 309 patients with PSC, 51 with primary biliary cholangitis (PBC), and 120 healthy blood donors (BD) were analyzed. For the survival analysis in PSC, the outcome was defined as liver-transplantation-free survival during the follow-up. Positive PR3-ANCA levels were found in 74/309 (24.0%) of patients with PSC. No BDs and one patient with PBC demonstrated PR3-ANCA positivity. PR3-ANCAs were revealed as independent predictors for a poor PSC outcome (study endpoint: liver transplantation/death, log-rank test, p = 0.02). PR3-ANCA positivity, lower albumin levels, and higher bilirubin concentrations were independent risks of a poor survival (Cox proportional-hazards regression analysis, p < 0.05). The Mayo risk score for PSC was associated with PR3-ANCA positivity (p = 0.01) and the disease severity assessed with a model of end-stage liver disease (MELD) and extended MELD-Na (p < 0.05). PR3-ANCAs detected by a third-generation ELISA are diagnostic and prognostic markers for PSC. Their wider use could help to identify patients who are at-risk of a more severe disease. KW - primary sclerosing cholangitis KW - antibodies against serine protease proteinase-3 KW - survival KW - health-related quality of life KW - liver biochemistry KW - disease severity Y1 - 2022 U6 - https://doi.org/10.3390/diagnostics12112682 SN - 2075-4418 VL - 12 IS - 11 ER - TY - GEN A1 - Burdukiewicz, Michał A1 - Sidorczuk, Katarzyna A1 - Rafacz, Dominik A1 - Pietluch, Filip A1 - Chilimoniuk, Jarosław A1 - Rödiger, Stefan A1 - Przemysław, Gagat T1 - Proteomic Screening for Prediction and Design of Antimicrobial Peptides with AmpGram T2 - International Journal of Molecular Sciences Y1 - 2020 U6 - https://doi.org/10.3390/ijms21124310 SN - 1422-0067 SN - 1661-6596 VL - 21 IS - 12 ER - TY - GEN A1 - Herrmann, Anna A1 - Rödiger, Stefan A1 - Schmidt, Carsten A1 - Schierack, Peter A1 - Schedler, Uwe T1 - Spatial Separation of Microbeads into Detection Levels by a Bioorthogonal Porous Hydrogel for Size-Selective Analysis und Increased Multiplexicity T2 - Analytical chemistry Y1 - 2019 U6 - https://doi.org/10.1021/acs.analchem.9b01586. SN - 1520-6882 SN - 0003-2700 VL - 91 IS - 13 SP - 8484 EP - 8491 ER - TY - GEN A1 - Schmidt, Carsten A1 - Schierack, Peter A1 - Gerber, Ulrike A1 - Schröder, Christian A1 - Choi, Youngeun A1 - Bald, Ilko A1 - Lehmann, Werner A1 - Rödiger, Stefan T1 - Streptavidin Homologues for Applications on Solid Surfaces at High Temperatures T2 - Langmuir Y1 - 2020 U6 - https://doi.org/10.1021/acs.langmuir.9b02339 SN - 1520-5827 SN - 0743-7463 VL - 36 IS - 2 SP - 628 EP - 636 ER - TY - GEN A1 - Reimann, Ronny A1 - Zeng, Bo A1 - Jakopec, Martin A1 - Burdukiewicz, Michał A1 - Petrick, Ingolf A1 - Schierack, Peter A1 - Rödiger, Stefan T1 - Classification of dead and living microalgae Chlorella vulgaris by bioimage informatics an machine learning T2 - Algal Research Y1 - 2020 U6 - https://doi.org/10.1016/j.algal.2020.101908 SN - 2211-9264 VL - 48 ER - TY - GEN A1 - Adefioye, Olusolabomi J. A1 - Weinreich, Jörg A1 - Rödiger, Stefan A1 - Schierack, Peter A1 - Olowe, Olugbenga Adekunle T1 - Phylogenetic Characterization and Multilocus Sequence Typing of Extended-Spectrum Beta Lactamase-Producing Escherichia coli from Food-Producing Animals, Beef, and Humans in Southwest Nigeria T2 - Microbial Drug Resistance Y1 - 2021 U6 - https://doi.org/10.1089/mdr.2019.0397 SN - 1931-8448 SN - 1076-6294 VL - 27 IS - 1 SP - 111 EP - 120 ER - TY - GEN A1 - Rödiger, Stefan A1 - Burdukiewicz, Michał A1 - Schierack, Peter T1 - chipPCR: an R package to pre-process raw data of amplification curves. Bioinformatics T2 - Bioinformatics Y1 - 2015 U6 - https://doi.org/10.1093/bioinformatics/btv205 SN - 1367-4803 SN - 1460-2059 VL - 31 IS - 17 SP - 2900 EP - 2902 ER -