TY - GEN A1 - Poraj-Kobielska, Marzena A1 - Peter, Sebastian A1 - Leonhardt, Sabrina A1 - Ullrich, René A1 - Scheibner, Katrin A1 - Hofrichter, Martin T1 - Immobilization of unspecific peroxygenases (EC 1.11.2.1) in PVA/PEG gel and hollow fiber modules T2 - Biochemical Engineering Journal N2 - The immobilization of enzymes has many advantages, such as higher stability, easier handling, and reuse of the catalyst. Here we report, for the first time, two effective methods for the immobilization of unspecific peroxygenase (UPO; EC 1.11.2.1). This biocatalyst type comprises heavily glycosylated heme-thiolate proteins that catalyze various biotechnologically relevant oxyfunctionalizations. Both the encapsulation in cryogel and the retention of the enzyme in hollow fiber modules were found to be efficient methods for their immobilization. After encapsulation, the enzyme still exhibited 60% of its initial activity. Interestingly, we did not find differences in the kinetic parameters of free and immobilized UPOs. In long-term experiments, the conversion of the pharmaceutical diclofenac with immobilized UPOs in different reactor types yielded between 62 mg and 154 mg of the major human drug metabolite 4′-hydroxydiclofenac. The maximal total turnover number was about 60-fold higher compared to the free enzyme. A test over 5 months showed that storage of encapsulated UPOs in non-polar solvents (e.g., cyclohexane) helps to preserve the enzyme stability and increases their relative activity (by about ∼150%, in the case of diclofenac hydroxylation). In addition to the hydrophilic substrate diclofenac, encapsulated UPOs also oxidized the hydrophobic model compound cyclohexane. KW - Peroxygenase Y1 - 2015 UR - http://www.sciencedirect.com/science/article/pii/S1369703X15000820 U6 - https://doi.org/10.1016/j.bej.2015.02.037 SN - 1369-703X VL - 98 SP - 144 EP - 150 ER - TY - GEN A1 - Daksha, Manaswi A1 - Derzsi, Aranka A1 - Wilczek, Sebastian A1 - Trieschmann, Jan A1 - Mussenbrock, Thomas A1 - Awakowicz, Peter A1 - Donkó, Zoltán A1 - Schulze, Julian T1 - The Effect of Realistic Heavy Particle Induced Secondary Electron Emission Coefficients on the Electron Power Absorption Dynamics in Single- and Dual-Frequency CCPs T2 - Plasma Sources Science and Technology Y1 - 2017 U6 - https://doi.org/10.1088/1361-6595/aa7c88 SN - 1361-6595 SN - 0963-0252 VL - 26 IS - 8 SP - 085006 ER - TY - GEN A1 - Wilczek, Sebastian A1 - Trieschmann, Jan A1 - Schulze, Julian A1 - Donko, Zoltan A1 - Brinkmann, Ralf Peter A1 - Mussenbrock, Thomas T1 - Disparity between current and voltage driven capacitively coupled radio frequency discharges T2 - Plasma Sources Science and Technology Y1 - 2018 U6 - https://doi.org/10.1088/1361-6595/aae5c1 SN - 1361-6595 SN - 0963-0252 VL - 27 IS - 12 ER - TY - GEN A1 - Klich, Maximilian A1 - Wilczek, Sebastian A1 - Janssen, Jesper Frederikus Jacobus A1 - Brinkmann, Ralf Peter A1 - Mussenbrock, Thomas A1 - Trieschmann, Jan T1 - Ion dynamics in capacitively coupled argon–xenon discharges T2 - Plasma Sources Science and Technology Y1 - 2021 U6 - https://doi.org/10.1088/1361-6595/ac02b0 SN - 1361-6595 VL - 30 IS - 6 ER - TY - RPRT A1 - Thonicke, Kirsten A1 - Rahner, Eva A1 - Arneth, Almut A1 - Bartkowski, Bartosz A1 - Bonn, Aletta A1 - Döhler, Christian A1 - Finger, Robert A1 - Freitag, Jens A1 - Grosch, Rita A1 - Grossart, Hans-Peter A1 - Grützmacher, Kim A1 - Hartman Scholz, Amber A1 - Häuser, Christoph A1 - Hickler, Thomas A1 - Hölker, Franz A1 - Jähnig, Sonja C. A1 - Jeschke, Jonathan A1 - Kassen, Rees A1 - Kastner, Thomas A1 - Kramer-Schadt, Stephanie A1 - Krug, Cornelia A1 - Lakner, Sebastian A1 - Loft, Lasse A1 - Matzdorf, Bettina A1 - Meakins, Felicity A1 - De Meester, Luc A1 - Monaghan, Michael T. A1 - Müller, Daniel A1 - Overmann, Jörg A1 - Quaas, Martin A1 - Radchuk, Viktoriia A1 - Reyer, Christopher A1 - Roos, Christian A1 - Scholz, Imme A1 - Schroer, Sibylle A1 - Sioen, Giles Bruno A1 - Sommer, Simone A1 - Sommerwerk, Nike A1 - Tockner, Klement A1 - Turk, Zachary A1 - Warner, Barbara A1 - Wätzold, Frank A1 - Wende, Wolfgang A1 - Veenstra, Tonjes A1 - Voort, Hein van de T1 - 10 Must-Knows aus der Biodiversitätsforschung Y1 - 2022 U6 - https://doi.org/10.5281/zenodo.6257476 PB - Potsdam-Institut für Klimafolgenforschung e.V. (PIK) CY - Potsdam ER - TY - GEN A1 - Duda, Niklas A1 - Nowak, Thorsten A1 - Hartmann, Markus A1 - Schadhauser, Michael A1 - Cassens, Björn A1 - Wägemann, Peter A1 - Nabeel, Muhammad A1 - Ripperger, Simon A1 - Herbst, Sebastian A1 - Meyer-Wegener, Klaus A1 - Mayer, Frieder A1 - Dressler, Falko A1 - Schröder-Preikschat, Wolfgang A1 - Kapitza, Rüdiger A1 - Robert, Jörg A1 - Thielecke, Jörn A1 - Weigel, Robert A1 - Kölpin, Alexander T1 - BATS: Adaptive Ultra Low Power Sensor Network for Animal Tracking T2 - Sensors Y1 - 2018 U6 - https://doi.org/10.3390/s18103343 SN - 1424-8220 VL - 18 IS - 10 ER - TY - GEN A1 - Kramm, Ulrike Ingrid A1 - Herrmann-Geppert, Iris A1 - Fiechter, Sebastian A1 - Zehl, Gerald A1 - Zizak, Ivo A1 - Dorbandt, Iris A1 - Schmeißer, Dieter A1 - Bogdanoff, Peter T1 - Effect of iron carbide formation on the number of active sites in Fe-N-C catalysts for the oxygen reduction reaction in acidic media T2 - Journals of materials chemistry A KW - Oxygen reduction reaction (ORR) KW - Fe-N-C catalyst KW - acidic media KW - high-temperature X-ray diffraction (HT-XRD) KW - thermogravimetry coupled with mass-spectroscopy (TG-MS) Y1 - 2014 U6 - https://doi.org/10.1039/c3ta13821f SN - 2050-7488 SN - 2050-7496 IS - 8 SP - 2663 EP - 2670 ER - TY - GEN A1 - Wilczek, Sebastian A1 - Trieschmann, Jan A1 - Schulze, Julian A1 - Schüngel, Edmund A1 - Brinkmann, Ralf Peter A1 - Derzsi, Aranka A1 - Korolov, Ihor A1 - Donkó, Zoltán A1 - Mussenbrock, Thomas T1 - The Effect of the Driving Frequency on the Confinement of Beam Electrons and Plasma Density in Low Pressure Capacitive Discharges T2 - Plasma Sources Science and Technology Y1 - 2015 U6 - https://doi.org/10.1088/0963-0252/24/2/024002 SN - 0963-0252 SN - 1361-6595 VL - 24 IS - 2 SP - 024002 ER - TY - GEN A1 - Lehnert, Lukas W. A1 - Thies, Boris A1 - Trachte, Katja A1 - Achilles, Sebastian A1 - Osses, Pablo A1 - Baumann, Karen A1 - Schmidt, Jakob A1 - Samolov, Elena A1 - Jung, Patrick A1 - Leinweber, Peter A1 - Karsten, Ulf A1 - Büdel, Burkhard A1 - Bendix, Jörg T1 - A Case Study on Fog/Low Stratus Occurrence at Las Lomitas, Atacama Desert (Chile) as a Water Source for Biological Soil Crusts T2 - Aerosol and Air Quality Research N2 - The Atacama Desert is well known for the high occurrence of large-scale fog (spatial extents: hundreds of kilometers) emerging as low stratus (LST) decks over the Pacific Ocean. By contrast, the small-scale and heterogeneous occurrence of small-scale fog (hundreds of meters) particularly during summers is widely unconsidered. However, these events are important for the local vegetation and particularly for the biological soil crusts (BSC) that are widely distributed in this extreme ecosystem. Consequently, a case study in a typical fog oasis in the Pan de Azúcar National Park was conducted to test the feasibility combining field measurements, drone profiling, remote sensing and numerical modeling (i) to investigate fog-type specific differences regarding dynamics, physical properties and formation, (ii) to test the applicability of remote sensing technology for fog monitoring based on existing low-resolution and a proposed new high-resolution product and (iii) to estimate the related fog water input to BSCs. Two types of fog were observed. The well-known fog/LST deck emerging from the Pacific Ocean with high water path and large spatial extent was the first type. Fog of the second type was patchier, small-scale and not necessarily connected to the LST over the ocean. Instead, fog formation of the second type was related to thermal breeze systems, which produced shallow clouds containing less water than those of type 1. In general, such small-scale fog events were not captured well by existing remote sensing products but could be detected with the proposed new high-resolution product which provided promising results. Both fog types were important water resources for the BSCs, with approximately 8% to 24% of the fog water flux available to the BSCs at the surface. The results indicated the feasibility of the proposed methods’ pool to estimate the water budget of BSCs with a high spatial resolution in the future. KW - Orographic fog KW - Landsat KW - WRF-modeling KW - Biological soil crusts KW - Vertical fog droplet spectra Y1 - 2017 U6 - https://doi.org/10.4209/aaqr.2017.01.0021 SN - 1680-8584 SN - 2071-1409 VL - 18 IS - 1 SP - 254 EP - 269 ER - TY - CHAP A1 - Langendörfer, Peter A1 - Adam, Sebastian T1 - A Graphical Tool for Specification, Rapid Prototyping and Implementation of Location Based Services Y1 - 2006 ER - TY - GEN A1 - Wilczek, Sebastian A1 - Schulze, Julian A1 - Brinkmann, Ralf Peter A1 - Donkó, Zoltan A1 - Trieschmann, Jan A1 - Mussenbrock, Thomas T1 - Electron dynamics in low pressure capacitively coupled radio frequency discharges T2 - Journal of Applied Physics Y1 - 2020 U6 - https://doi.org/10.1063/5.0003114.1 VL - 127 IS - 18 ER - TY - GEN A1 - Öngüner, Emir A1 - Dittmar, Mirko A1 - Meyer, Peter A1 - Merbold, Sebastian A1 - Egbers, Christoph ED - Egbers, Christoph T1 - Cross-sectional PIV analysis in turbulent pipe flow at high Reynolds number T2 - Experimentelle Strömungsmechanik, 24. Fachtagung 6.-8. September 2016 Cottbus Y1 - 2016 SN - 978-3-9816764-2-6 SP - S. 31 PB - German Association for Laser Anemometry GALA e.V. CY - Karlsruhe ER - TY - GEN A1 - Neyret, Margot A1 - Le Provost, Gaëtane A1 - Boesing, Andrea Larissa A1 - Schneider, Florian D. A1 - Baulechner, Dennis A1 - Bergmann, Joana A1 - Vries, Franciska T. de A1 - Fiore-Donno, Anna Maria A1 - Geisen, Stefan A1 - Goldmann, Kezia A1 - Merges, Anna A1 - Saifutdinov, Ruslan A. A1 - Simons, Nadja K. A1 - Tobias, Joseph A. A1 - Zaitsev, Andrey S. A1 - Gossner, Martin M. A1 - Jung, Kirsten A1 - Kandeler, Ellen A1 - Krauss, Jochen A1 - Penone, Caterina A1 - Schloter, Michael A1 - Schulz, Stefanie A1 - Staab, Michael A1 - Wolters, Volkmar A1 - Apostolakis, Antonios A1 - Birkhofer, Klaus A1 - Boch, Steffen A1 - Boeddinghaus, Runa S. A1 - Bolliger, Ralph A1 - Bonkowski, Michael A1 - Buscot, François A1 - Dumack, Kenneth A1 - Fischer, Markus A1 - Gan, Huei Ying A1 - Heinze, Johannes A1 - Hölzel, Norbert A1 - John, Katharina A1 - Klaus, Valentin H. A1 - Kleinebecker, Till A1 - Marhan, Sven A1 - Müller, Jörg A1 - Renner, Swen C. A1 - Rillig, Matthias C. A1 - Schenk, Noëlle V. A1 - Schöning, Ingo A1 - Schrumpf, Marion A1 - Seibold, Sebastian A1 - Socher, Stephanie A. A1 - Solly, Emily F. A1 - Teuscher, Miriam A1 - Kleunen, Mark van A1 - Wubet, Tesfaye A1 - Manning, Peter T1 - A slow-fast trait continuum at the whole community level in relation to land-use intensification T2 - Nature Communications N2 - AbstractOrganismal functional strategies form a continuum from slow- to fast-growing organisms, in response to common drivers such as resource availability and disturbance. However, whether there is synchronisation of these strategies at the entire community level is unclear. Here, we combine trait data for 2800 above- and belowground taxa from 14 trophic guilds spanning a disturbance and resource availability gradient in German grasslands. The results indicate that most guilds consistently respond to these drivers through both direct and trophically mediated effects, resulting in a ‘slow-fast’ axis at the level of the entire community. Using 15 indicators of carbon and nutrient fluxes, biomass production and decomposition, we also show that fast trait communities are associated with faster rates of ecosystem functioning. These findings demonstrate that ‘slow’ and ‘fast’ strategies can be manifested at the level of whole communities, opening new avenues of ecosystem-level functional classification. KW - General Physics and Astronomy KW - General Biochemistry KW - General Chemistry KW - Multidisciplinary KW - Genetics and Molecular Biology Y1 - 2024 U6 - https://doi.org/10.1038/s41467-024-45113-5 SN - 2041-1723 VL - 15 IS - 1 ER - TY - GEN A1 - Ranjan, Amit A1 - Scholz, Julia A1 - Semmler, Torsten A1 - Wieler, Lothar H. A1 - Ewers, Christa A1 - Müller, Stefanie A1 - Pickard, Derek J. A1 - Schierack, Peter A1 - Tedin, Karsten A1 - Ahmed, Niyaz A1 - Schaufler, Katharina A1 - Guenther, Sebastian T1 - ESBL-plasmid carriage in E. coli enhances in vitro bacterial competition fitness and serum resistance in some strains of pandemic sequence types without overall fitness cost T2 - Gut pathogens Y1 - 2018 U6 - https://doi.org/10.1186/s13099-018-0243-z SN - 1757-4749 VL - 10 IS - 24 ER - TY - GEN A1 - Mohsin, Mashkoor A1 - Guenther, Sebastian A1 - Schierack, Peter A1 - Tedin, Karsten A1 - Wieler, Lothar H. T1 - Probiotic Escherichia coli Nissle 1917 reduces growth, Shiga toxin expression, release and thus cytotoxicity of enterohemorrhagic Escherichia coli T2 - International Journal of Medical Microbiology Y1 - 2015 U6 - https://doi.org/10.1016/j.ijmm.2014.10.003 SN - 1618-0607 SN - 1438-4221 VL - 305 IS - 1 SP - 20 EP - 26 ER - TY - GEN A1 - Guenther, Sebastian A1 - Groth, Ingrid A1 - Schierack, Peter A1 - Grabley, Susanne A1 - Munder, Thomas T1 - Direct detection of Kitasatospora species with a chaperone oligonucleotide microarray method lacking PCR amplification T2 - Journal of basic microbiology Y1 - 2008 U6 - https://doi.org/10.1002/jobm.200800038 SN - 1521-4028 SN - 0233-111X VL - 48 IS - 4 SP - 315 EP - 318 ER - TY - GEN A1 - Mohsin, Mashkoor A1 - Raza, Shahbaz A1 - Roschanski, Nicole A1 - Guenther, Sebastian A1 - Ali, Aamir A1 - Schierack, Peter T1 - Description of the First Escherichia coli Clinical Isolate Harboring the Colistin Resistance Gene mcr-1 from the Indian Subcontinent T2 - Antimicrobial Agents and Chemotherapy Y1 - 2016 U6 - https://doi.org/10.1128/AAC.01945-16 SN - 1098-6596 SN - 0066-4804 VL - 61 IS - 1 ER - TY - GEN A1 - Zöllner, Marvin Hartwig A1 - Zaumseil, Peter A1 - Wilkens, Henrik A1 - Gevers, Sebastian A1 - Wollschläger, Joachim A1 - Bäumer, Marcus A1 - Xie, Ya-Hong A1 - Niu, Gang A1 - Schroeder, Thomas T1 - Stoichiometry-structure correlation of epitaxial Ce1-xPrxO2-δ (x = 0-1) thin films on Si(111) T2 - Journal of Crystal Growth Y1 - 2012 SN - 0022-0248 VL - 355 IS - 1 SP - 159 EP - 165 ER - TY - GEN A1 - Guenther, Sebastian A1 - Schierack, Peter A1 - Grobbel, Mirjam A1 - Lübke-Becker, Antina A1 - Wieler, Lothar H. A1 - Ewers, Christa T1 - Real-time PCR assay for the detection of species of the genus Mannheimia. T2 - Journal of Microbiological Methods Y1 - 2008 U6 - https://doi.org/10.1016/j.mimet.2008.05.008 SN - 1872-8359 SN - 0167-7012 VL - 75 IS - 1 SP - 75 EP - 80 ER - TY - GEN A1 - Willitzki, Annika A1 - Lorenz, Sebastian A1 - Hiemann, Rico A1 - Guttek, Karina A1 - Goihl, Alexander A1 - Hartig, Roland A1 - Conrad, Karsten A1 - Feist, Eugen A1 - Sack, Ulrich A1 - Schierack, Peter A1 - Heiserich, Lisa A1 - Eberle, Caroline A1 - Peters, Vanessa A1 - Roggenbuck, Dirk A1 - Reinhold, Dirk T1 - Fully automated analysis of chemically induced γH2AX foci in human peripheral blood mononuclear cells by indirect immunofluorescence T2 - Cytometry Part A Y1 - 2013 U6 - https://doi.org/10.1002/cyto.a.22350 SN - 1552-4930 SN - 1552-4922 VL - 83 IS - 11 SP - 1017 EP - 1026 ER -