TY - PAT A1 - Brinkmann, Ralf Peter A1 - Oberrath, Jens A1 - Awakowicz, Peter A1 - Lapke, Martin A1 - Musch, Thomas A1 - Mussenbrock, Thomas A1 - Rolfes, Ilona A1 - Schulz, Christian A1 - Storch, Robert A1 - Styrnoll, Tim A1 - Zietz, Christian T1 - Device and Use of the Device for Measuring the Density and/or the Electron Temperature and/or the Collision Frequency of a Plasma Y1 - 2011 ER - TY - GEN A1 - Schulz, Christian M. A1 - Schneider, Erich A1 - Kohlbecher, Stefan A1 - Hapfelmeier, Alexander A1 - Heuser, Fabian T1 - The influence of anaesthetists’ experience on workload, performance and visual attention during simulated critical incidents T2 - Journal of Clinical Monitoring and Computing Y1 - 2014 U6 - https://doi.org/10.1007/s10877-013-9443-8 SN - 1387-1307 SN - 1573-2614 VL - 28 IS - 5 SP - 475 EP - 480 ER - TY - GEN A1 - Lehnen, Nadine A1 - Heuser, Fabian A1 - Sağlam, Murat A1 - Schulz, Christian M. A1 - Wagner, Klaus J. A1 - Taki, Masakatsu A1 - Kochs, Eberhard F. A1 - Jahn, Klaus A1 - Schneider, Erich T1 - Opioid-induced nausea involves a vestibular problem preventable by head-rest T2 - PLoS one Y1 - 2015 U6 - https://doi.org/10.1371/journal.pone.0135263 SN - 1932-6203 VL - 10 IS - 8 SP - e0135263 ER - TY - GEN A1 - Schulz, Christian M. A1 - Skrzypczak, M. A1 - Schneider, Erich A1 - Hapfelmeier, Alexander A1 - Martin, J. A1 - Kochs, Eberhard F. A1 - Schneider, G. T1 - Assessment of subjective workload in an anaesthesia simulator environment: reliability and validity T2 - European Journal of Anaesthesiology Y1 - 2011 SN - 0265-0215 VL - 28 IS - 7 SP - 502 EP - 505 ER - TY - GEN A1 - Heuser, Fabian A1 - Schulz, Christian A1 - Sağlam, Murat A1 - Ramaioli, Cecilia A1 - Heuberger, Maria A1 - Wagner, Klaus J. A1 - Jahn, Klaus A1 - Schneider, Erich A1 - Brandt, Thomas A1 - Glasauer, Stefan A1 - Lehnen, Nadine T1 - Preventing opioid-induced nausea and vomiting: Rest your head and close your eyes? T2 - PloS one Y1 - 2017 U6 - https://doi.org/10.1371/journal.pone.0173925 SN - 1932-6203 VL - 12 IS - 3 SP - e0173925 ER - TY - CHAP A1 - Schmidt, Sindy A1 - Schulz, Christian T1 - Entwicklung eines Steuerungs- und Regelungsprogrammes für einen Prüfstand zur Prüfung von Feuerwehrpumpen T2 - Virtuelle Instrumente in der Praxis 2015 : Begleitband zum 20. VIP-Kongress N2 - Kurzdokumentation über die Programmierung einer Software, für die Anwendung automatischer Prüfroutinen, zur Prüfung von Feuerwehrpumpen, Armaturen und Saugschläuchen, unter Verwendung von LabVIEW als grafische Programmieroberfläche. N2 - Short documentation on software programming, to use automatic test routines, for testing of fire pumps, fittings and suction hoses, using LabVIEW as graphical programming interface. KW - Prüfstand KW - LabVIEW Y1 - 2015 SN - 978-3-8007-3669-0 SP - 79 EP - 81 PB - VDE Verlag GmbH CY - Berlin ER - TY - GEN A1 - Prill, Robert A1 - Singh, Jasvinder A. A1 - Seeber, Gesine H. A1 - Mai Nielsen, Sabrina A1 - Goodman, Susan A1 - Michel, Sven A1 - Kopkow, Christian A1 - Schulz, Robert A1 - Choong, Peter A1 - Hommel, Hagen T1 - Patient, physiotherapist and surgeon endorsement of the core domain set for total hip and total knee replacement in Germany: a study protocol for an OMERACT initiative T2 - BMJ open Y1 - 2020 U6 - https://doi.org/10.1136/bmjopen-2019-035207 SN - 2044-6055 VL - 10 IS - 6 ER - TY - GEN A1 - Schulz, Christian A1 - Krüger-Genge, Anne A1 - Lendlein, Andreas A1 - Küpper, Jan-Heiner A1 - Jung, Friedrich T1 - Potential Effects of Nonadherent on Adherent Human Umbilical Venous Endothelial Cells in Cell Culture T2 - International Journal of Molecular Science N2 - The adherence and shear-resistance of human umbilical venous endothelial cells (HUVEC) on polymers is determined in vitro in order to qualify cardiovascular implant materials. In these tests, variable fractions of HUVEC do not adhere to the material but remain suspended in the culture medium. Nonadherent HUVEC usually stop growing, rapidly lose their viability and can release mediators able to influence the growth and function of the adherent HUVEC. The aim of this study was the investigation of the time dependent behaviour of HUVEC under controlled nonadherent conditions, in order to gain insights into potential influences of these cells on their surrounding environment in particular adherent HUVEC in the context of in vitro biofunctionality assessment of cardiovascular implant materials. Data from adherent or nonadherent HUVEC growing on polystyrene-based cell adhesive tissue culture plates (TCP) or nonadhesive low attachment plates (LAP) allow to calculate the number of mediators released into the culture medium either from adherent or nonadherent cells. Thus, the source of the inflammatory mediators can be identified. For nonadherent HUVEC, a time-dependent aggregation without further proliferation was observed. The rate of apoptotic/dead HUVEC progressively increased over 90% within two days. Concomitant with distinct blebbing and loss of membrane integrity over time, augmented releases of prostacyclin (PGI2, up to 2.91 ± 0.62 fg/cell) and platelet-derived growth factor BB (PDGF-BB, up to 1.46 ± 0.42 fg/cell) were detected. The study revealed that nonadherent, dying HUVEC released mediators, which can influence the surrounding microenvironment and thereby the results of in vitro biofunctionality assessment of cardiovascular implant materials. Neglecting nonadherent HUVEC bears the risk for under- or overestimation of the materials endothelialization potential, which could lead to the loss of relevant candidates or to uncertainty with regard to their suitability for cardiac applications. One approach to minimize the influence from nonadherent endothelial cells could be their removal shortly after observing initial cell adhesion. However, this would require an individual adaptation of the study design, depending on the properties of the biomaterial used. KW - human venous endothelial cells KW - adherent KW - non-adherent KW - viability KW - mediator release Y1 - 2021 UR - https://www.mdpi.com/1422-0067/22/3/1493 U6 - https://doi.org/10.3390/ijms22031493 VL - 22 IS - 3 ER - TY - GEN A1 - Prill, Robert A1 - Singh, Jasvinder A. A1 - Seeber, Gesine H. A1 - Mai Nielsen, Sabrina A1 - Goodman, Susan A1 - Michel, Sven A1 - Kopkow, Christian A1 - Schulz, Robert A1 - Choong, Peter A1 - Hommel, Hagen T1 - Endorsement des OMERACT Core Domain Sets für Hüft- und Kniegelenkersatz: ein Survey unter Patienten, Physiotherapeuten und Orthopäden in Deutschland – ein Studienprotokoll T2 - 4. Forschungssymposium Physiotherapie, FSPT2019 Abstractband Y1 - 2019 UR - http://www.dgptw.org/symposien/fspt-2019/fspt2019-abstractband SP - 45 EP - 46 ER - TY - GEN A1 - Schulz, Christian A1 - Jung, Friedrich A1 - Küpper, Jan-Heiner T1 - Inhibition of phase-1 biotransformation and cytostatic effects of diphenyleneiodonium on hepatoblastoma cell line HepG2 and a CYP3A4-overexpressing HepG2 cell clone T2 - Clinical Hemorheology and Microcirculation N2 - Cell-based in vitro liver models are an important tool in the development and evaluation of new drugs in pharmacological and toxicological drug assessment. Hepatic microsomal enzyme complexes, consisting of cytochrome P450 oxidoreductase (CPR) and cytochrome P450 monooxygenases (CYPs), play a decisive role in catalysing phase-1 biotransformation of pharmaceuticals and xenobiotics. For a comprehensive understanding of the phase-1 biotransformation of drugs, the availability of well-characterized substances for the targeted modulation of in vitro liver models is essential. In this study, we investigated diphenyleneiodonium (DPI) for its ability to inhibit phase-1 enzyme activity and further its toxicological profile in an in vitro HepG2 cell model with and without recombinant expression of the most important drug metabolization enzyme CYP3A4. Aim of the study was to identify effective DPI concentrations for CPR/CYP activity modulation and potentially associated dose and time dependent hepatotoxic effects. The cells were treated with DPI doses up to 5,000nM (versus vehicle control) for a maximum of 48 h and subsequently examined for CYP3A4 activity as well as various toxicological relevant parameters such as cell morphology, integrity and viability, intracellular ATP level, and proliferation. Concluding, the experiments revealed a time- and concentration-dependent DPI mediated partial and complete inhibition of CYP3A4 activity in CYP3A4 overexpressing HepG2-cells (HepG2-CYP3A4). Other cell functions, including ATP synthesis and consequently the proliferation were negatively affected in both in vitro cell models. Since neither cell integrity nor cell viability were reduced, the effect of DPI in HepG2 can be assessed as cytostatic rather than cytotoxic. KW - Phase-1 KW - biotransformation KW - CYP KW - cytochrome P450 monooxygenase KW - CYP3A4 KW - diphenyleneiodonium KW - DPI KW - HepG2 KW - HepG2-CYP3A4 KW - hepatocytes KW - NADPH-cytochrome P450 oxidoreductase KW - POR KW - CPR Y1 - 2021 UR - https://content.iospress.com/articles/clinical-hemorheology-and-microcirculation/ch219117 U6 - https://doi.org/10.3233/CH-219117 SN - 1875-8622 SN - 1386-0291 VL - 79 IS - 1 SP - 231 EP - 243 ER -