TY - GEN A1 - Schmidt, Carsten A1 - Kammel, Anne A1 - Tanner, Julian A. A1 - Kinghorn, Andrew B. A1 - Khan, Muhammad Moman A1 - Lehmann, Werner A1 - Menger, Marcus A1 - Schedler, Uwe A1 - Schierack, Peter A1 - Rödiger, Stefan T1 - A Multiparametic Fluorescence Assay for Screening Aptamer-Protein Interactions Based on Microbeads T2 - Scientific Reports Y1 - 2022 U6 - https://doi.org/10.1038/s41598-022-06817-0 SN - 2045-2322 VL - 12 ER - TY - GEN A1 - Ehrling, Sebastian A1 - Senkovska, Irena A1 - Efimova, Anastasia A1 - Bon, Volodymyr A1 - Abylgazina, Leila A1 - Petkov, Petko A1 - Evans, Jack D. A1 - Attallah, Ahmed Gamal A1 - Wharmby, Michael Thomas A1 - Roslova, Maria A1 - Huang, Zhehao A1 - Tanaka, Hideki A1 - Wagner, Andreas A1 - Schmidt, Peer A1 - Kaskel, Stefan T1 - Temperature Driven Transformation of the Flexible Metal-Organic Framework DUT-8(Ni) T2 - Chemistry - a European journal N2 - DUT-8(Ni) metal-organic framework belongs to the family of flexible pillared layer materials. The desolvated framework can be obtained in the open pore form (op) or in the closed pore form (cp), depending on the crystal size regime. In the present work, we report on the behaviour of desolvated DUT-8(Ni) at elevated temperatures. For both, op and cp variants, heating causes a structural transition, leading to an new, crystalline compound, containing two interpenetrated networks. The state of the framework before transition (op vs. cp) influences the transition temperature: the small particles of the op phase transform at significantly lower temperature in comparison to the macroparticles of the cp phase, transforming close to the decomposition temperature. The new compound, confined closed pore phase (ccp), was characterized by powder X-ray diffraction and spectroscopic techniques, such as IR, EXAFS, and positron annihilation lifetime spectroscopy (PALS). Thermal effects of structural cp to ccp transitions were studied using differential scanning calorimetry (DSC), showing an overall exothermic effect of the process, involving bond breaking and reformation. Theoretical calculations reveal the energetics, driving the observed temperature induced phase transition. KW - Metal organic framework (MOF) KW - Thermal analysis KW - Crystal structure KW - Phase transition Y1 - 2022 UR - https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/chem.202201281 U6 - https://doi.org/10.1002/chem.202201281 SN - 1521-3765 VL - 28 IS - 55 SP - 1 EP - 10 ER - TY - RPRT A1 - Böttger, Stefan A1 - Felgentreff, Bernd A1 - Hesse, Gerold A1 - Hloucal, Martin-Joseph A1 - Leßmann, Dieter A1 - Mix, Sebastian A1 - Roselt, Kersten A1 - Safarik, Mathias A1 - Schmidt, Jörg A1 - Steffan, Christoph A1 - Uhlmann, Wilfried T1 - Seethermie - Innovative Wärmeversorgung aus Tagebaurestseen - Schlussbericht KW - Seethermie Y1 - 2021 UR - https://www.innovationsregion-mitteldeutschland.com/wp-content/uploads/2021/07/20210723_Schlussbericht-Seethermie_Langfassung.pdf CY - Leipzig ER - TY - GEN A1 - Böhler, Stefan A1 - Rosencrantz, Sophia A1 - Wolf, Karina A1 - Heinemann, Robert A1 - Schmidt, Peer A1 - Ganster, Johannes A1 - Büsse, Thomas A1 - Balko, Jens A1 - Rosencrantz, Ruben R. T1 - Active protease formulation in commodity polymers withstands melt processing into compounds and blown films T2 - Materials Today Communications N2 - Integrating enzymes into thermoplastic polymers is challenging due to their lack of robustness with respect to temperature and shear fields during conventional melt processing. In the present study, blown films from low-density polyethylene (LDPE) were prepared containing a technical protease from Bacillus sp. First, LDPE/protease compounds were produced followed by blown film extrusion, both processes at melt mass temperatures of 130 °C or higher. Enzyme activity was proven, both for the LDPE/protease compound and the blown film. The highest enzyme activity in the compound was determined for processing at 132 °C and a screw speed of 75 rpm. The influence of melt temperature and shear fields was studied in detail. Enzyme activities were determined for melt temperatures up to 160 °C and for screw speeds ranging from 75 to 300 rpm during compounding by twin-screw extrusion. The process was also applied for biobased and biodegradable polyesters, where similar protease activity after compounding was verified. Electron microscopy, X-ray diffraction, nuclear magnetic resonance spectroscopy and differential scanning calorimetry served to analyze components and morphology of the enzyme formulation used here. It is proposed that the porous morphology of the protease particles is beneficial for the enzyme to remain active after processing. Additionally, the polymer matrix surrounding the particles protects the protease at elevated temperatures, which can be attributed to thermal insulation. Thus, the right combination of a suited technical enzyme formulation with appropriate mild melt compounding conditions allows enzymes to be incorporated into thermoplastics and retain their activity. This opens the way to use the abundant biological functions of enzymes in thermoplastic applications. KW - Thermal analysis KW - X-ray diffraction KW - Crystal structure Y1 - 2023 UR - https://www.sciencedirect.com/science/article/pii/S2352492822018591 U6 - https://doi.org/10.1016/j.mtcomm.2022.105018 SN - 2352-4928 VL - 34 SP - 1 EP - 9 ER - TY - GEN A1 - Geithe, Christiane A1 - Zeng, Bo A1 - Schmidt, Carsten A1 - Dinter, Franziska A1 - Roggenbuck, Dirk A1 - Lehmann, Werner A1 - Dame, Gregory A1 - Schierack, Peter A1 - Hanack, Katja A1 - Rödiger, Stefan T1 - A multiplex microchamber diffusion assay for the antibody-based detection of microRNAs on randomly ordered microbeads T2 - Biosensors and Bioelectronics: X Y1 - 2024 U6 - https://doi.org/10.1016/j.biosx.2024.100484 SN - 2590-1370 VL - 18 (2024) SP - 1 EP - 7 PB - Elsevier BV ER - TY - RPRT A1 - Behrens, Bernd-Arno A1 - Krimm, Richard A1 - Fries, Stefan A1 - Härtel, Sebastian A1 - Schmidt, Alexander T1 - Eigenschaften individuell gefertigter Bauteile für Umformanlagen N2 - Die Dauerschwingfestigkeit ist eine wichtige sowie für die Auslegung von zyklisch belasteten Bauteilen in Umformmaschinen notwendige Materialkenngröße. Diese Kenngröße ist nicht nur material- sondern auch fertigungsprozessabhängig. Aufgrund der rapiden Entwicklung von additiven Fertigungsanlagen und Werkstoffen mangelt es an hinreichenden Erfahrungswerten zu den Betriebseigenschaften von additiv gefertigten Bauteilen. Daher ist es wichtig, die Eignung von additiv gefertigten Bauteilen für Umformmaschinen im Vorfeld technologisch und wissenschaftlich zu untersuchen. Die Ermittlung der Dauerschwingfestigkeit von additiv gefertigten Komponenten für Umformmaschinen liefert außerdem einen Mehrwert für die konstruktive Auslegung anderer AM-Bauteile mit einem vergleichbaren Belastungskollektiv und ermöglicht eine Topologieoptimierung von Komponenten von Umformmaschinen. Im Rahmen dieses Forschungsvorhabens wurde die Dauerschwingfestigkeit additiv gefertigter Bauteile in Abhängigkeit von der Gesamtheit der Herstellungsparameter eines WAAM-Prozesses sowie diverser Nachbehandlungsmethoden erfolgreich anhand des Fallbeispiels Pleuel ermittelt. Außerdem wurde die Anwendbarkeit von WAAM-Prozessen zur Herstellung von individuellen Anlagenbauteilen für den Bereich Umformmaschinen erfolgreich belegt. Mit den ermittelten Dauerschwingfestigkeitswerten konnte eine Grundlage zur Auslegung weiterer AM-Bauteile mit vergleichbaren Belastungskollektiv sowie Topologieoptimierung genannter Bauteile geschaffen werden. Die Untersuchungen erfolgten an Laborproben geeigneter Geometrie in Bezug auf das Realbauteil und unter Gewährleistung der thermischen Übertragbarkeit auf Realbauteile. Abschließend ist mittels eines skalierten Demonstratorbauteils die Machbarkeit nachgewiesen worden. Y1 - 2023 SN - 978-3-86776-660-9 CY - Hannover ER - TY - GEN A1 - Hall, Braeden A1 - Schmidt, Kathleen A1 - Wagge, Jordan A1 - Lewis, Savannah C. A1 - Weissgerber, Sophia C. A1 - Kiunke, Felix A1 - Pfuhl, Gerit A1 - Stieger, Stefan A1 - Tran, Ulrich S. A1 - Barzykowski, Krystian A1 - Bogatyreva, Natalia A1 - Kowal, Marta A1 - Massar, KarlIJn A1 - Pernerstofer, Felizitas A1 - Sorokowski, Piotr A1 - Voracek, Martin A1 - Chartier, Christopher R. A1 - Brandt, Mark J. A1 - Grahe, Jon E. A1 - Özdoğru, Asil A. A1 - Andreychik, Michael R. A1 - Chen, Sau-Chin A1 - Evans, Thomas R. A1 - Hautekiet, Caro A1 - IJzerman, Hans A1 - Kačmár, Pavol A1 - Krafnick, Anthony J. A1 - Musser, Erica D. A1 - Vergauwe, Evie A1 - Werner, Kaitlyn M. A1 - Aczel, Balazs A1 - Arriaga, Patrícia A1 - Batres, Carlota A1 - Beaudry, Jennifer L. A1 - Cova, Florian A1 - Ďurbisová, Simona A1 - Cramblet Alvarez, Leslie D. A1 - Feldman, Gilad A1 - Godbersen, Hendrik A1 - Gottfried, Jaroslav A1 - Haeffel, Gerald J. A1 - Hartanto, Andree A1 - Isloi, Chris A1 - McFall, Joseph P. A1 - Milyavskaya, Marina A1 - Moreau, David A1 - Nosáľová, Ester A1 - Papaioannou, Kostas A1 - Ruiz-Fernandez, Susana A1 - Schrötter, Jana A1 - Storage, Daniel A1 - Vezirian, Kevin A1 - Volz, Leonhard A1 - Weisberg, Yanna J. A1 - Xiao, Qinyu A1 - Awlia, Dana A1 - Branit, Hannah W. A1 - Dunn, Megan R. A1 - Groyecka-Bernard, Agata A1 - Haneda, Ricky A1 - Kielinska, Julita A1 - Kolle, Caroline A1 - Lubomski, Paweł A1 - Miller, Alexys M. A1 - Mækelæ, Martin J. A1 - Pantazi, Mytro A1 - Ribeiro, Rafael R. A1 - Ross, Robert M. A1 - Sorokowska, Agnieszka A1 - Aberson, Christopher L. A1 - Vassiliou, Xanthippi Alexi A1 - Baker, Bradley J. A1 - Bognar, Miklos A1 - Cong, Chin Wen A1 - Danvers, Alex F. A1 - Davis, William E. A1 - Dranseika, Vilius A1 - Dumbravă, Andrei A1 - Farmer, Harry A1 - Field, Andy P. A1 - Forscher, Patrick S. A1 - Graton, Aurélien A1 - Hajdu, Nandor A1 - Howlett, Peter A. A1 - Kabut, Radosław A1 - Larsen, Emmett M. A1 - Lee, Sean T. H. A1 - Legate, Nicole A1 - Levitan, Carmel A. A1 - Levy, Neil A1 - Lu, Jackson G. A1 - Misiak, Michał A1 - Morariu, Roxana E. A1 - Novak, Jennifer A1 - Pronizius, Ekaterina A1 - Prusova, Irina A1 - Rathnayake, Athulya S. A1 - Romanova, Marina O. A1 - Röer, Jan P. A1 - Sampaio, Waldir M. A1 - Schild, Christoph A1 - Schulte-Mecklenbeck, Michael A1 - Stephen, Ian D. A1 - Szecsi, Peter A1 - Takacs, Elizabeth A1 - Teeter, Julia N. A1 - Thiele-Evans, Elian H. A1 - Valeiro-Paterlini, Julia A1 - Vilares, Iris A1 - Villafana, Louise A1 - Wang, Ke A1 - Wu, Raymond A1 - Álvarez-Solas, Sara A1 - Moshontz, Hannah A1 - Buchanan, Erin M. T1 - Registered Replication Report: A Large Multilab Cross-Cultural Conceptual Replication of Turri et al. (2015) T2 - Advances in Methods and Practices in Psychological Science Y1 - 2024 U6 - https://doi.org/10.1177/25152459241267902 SN - 2515-2459 VL - 7 IS - 4 SP - 1 EP - 38 PB - SAGE Publications ER -