TY - GEN A1 - Sowa, Mandy A1 - Reddig, Annika A1 - Schierack, Peter A1 - Reinhold, Dirk A1 - Roggenbuck, Dirk T1 - Phosphorylated histone 2AX foci determination in capillary blood mononuclear cells T2 - Journal of Laboratory and Precision Medicine Y1 - 2018 U6 - https://doi.org/10.21037/jlpm.2018.04.02 SN - 2519-9005 VL - 3 ER - TY - GEN A1 - Schneider, Jens A1 - Weiss, Romano A1 - Ruhe, Madeleine A1 - Jung, Tobias A1 - Roggenbuck, Dirk A1 - Stohwasser, Ralf A1 - Schierack, Peter A1 - Rödiger, Stefan T1 - Open source bioimage informatic tools for the analysis of DNA damage and associated biomarkers T2 - Journal of Laboratory and Precision Medicine KW - bioimage informatics KW - DNA damage response (DDR) KW - double-strand break (DSB) KW - immunofluorescence microscopy KW - open source software KW - proteasome activator PA28gamma Y1 - 2019 U6 - https://doi.org/10.21037/jlpm.2019.04.05 SN - 2519-9005 VL - 4 ER - TY - GEN A1 - Ruhe, Madeleine A1 - Rabe, Dominik A1 - Jurischka, Christoph A1 - Schröder, Julia A1 - Schierack, Peter A1 - Deckert, P. Markus A1 - Rödiger, Stefan T1 - Molecular biomarkers of DNA damage in diffuse large-cell lymphoma-a review T2 - Journal of Laboratory and Precision Medicine Y1 - 2019 U6 - https://doi.org/10.21037/jlpm.2019.01.01 SN - 2519-9005 VL - 4 ER - TY - GEN A1 - Umair, Muhammad A1 - Mohsin, Mashkoor A1 - Ali, Qasim A1 - Qamar, Muhammad U. A1 - Raza, Shahbaz A1 - Ali, Aamir A1 - Guenther, Sebastian A1 - Schierack, Peter T1 - Prevalence and Genetic Relatedness of Extended Spectrum-ß-Lactamase-Producing Escherichia coli Among Humans, Cattle, and Poultry in Pakistan T2 - Microbial drug resistance Y1 - 2019 U6 - https://doi.org/10.1089/mdr.2018.0450 SN - 1931-8448 SN - 1076-6294 VL - 25 IS - 9 SP - 1374 EP - 1381 ER - TY - GEN A1 - Wajid, Muhammad A1 - Awan, Asad Bashir A1 - Saleemi, Muhammad Kashif A1 - Weinreich, Jörg A1 - Schierack, Peter A1 - Sarwar, Yasra A1 - Ali, Aamir T1 - Multiple Drug Resistance and Virulence Profiling of Salmonella enterica Serovars Typhimurium and Enteritidis from Poultry Farms of Faisalabad, Pakistan T2 - Microbial Drug Resistance Y1 - 2019 U6 - https://doi.org/10.1089/mdr.2018.0121 SN - 1931-8448 SN - 1076-6294 VL - 25 IS - 1 SP - 133 EP - 142 ER - TY - GEN A1 - Akhtar, Nasrin A1 - Ghauri, M. A. A1 - Parveen, Sana A1 - Farooq, M. A1 - Ali, Aamir A1 - Schierack, Peter T1 - Comparative Analysis of Draft Genome Sequence of Rhodococcus sp. Eu-32 with Other Rhodococcus Species for Its Taxonomie Status and Sulfur Metabolism Potential T2 - Current microbiology Y1 - 2019 U6 - https://doi.org/10.1007/s00284-019-01737-1 SN - 1432-0991 SN - 0343-8651 VL - 76 IS - 10 SP - 1207 EP - 1214 ER - TY - GEN A1 - Olowe, Olugbenga Adekunle A1 - Adefioye, Olusolabomi J. A1 - Ajayeoba, T. A. A1 - Schiebel, Juliane A1 - Weinreich, Jörg A1 - Ali, Aamir A1 - Burdukiewicz, Michał A1 - Rödiger, Stefan A1 - Schierack, Peter T1 - Phylogenetic grouping and biofilm formation of Multidrug Resistant Escherichia coli Isolates from Humans, Animals and Food products in South-west Nigeria T2 - Scientific African Y1 - 2019 U6 - https://doi.org/10.1016/j.sciaf.2019.e00158 SN - 2468-2276 VL - 6 ER - TY - GEN A1 - Herrmann, Anna A1 - Rödiger, Stefan A1 - Schmidt, Carsten A1 - Schierack, Peter A1 - Schedler, Uwe T1 - Spatial Separation of Microbeads into Detection Levels by a Bioorthogonal Porous Hydrogel for Size-Selective Analysis und Increased Multiplexicity T2 - Analytical chemistry Y1 - 2019 U6 - https://doi.org/10.1021/acs.analchem.9b01586. SN - 1520-6882 SN - 0003-2700 VL - 91 IS - 13 SP - 8484 EP - 8491 ER - TY - GEN A1 - Awan, Asad Bashir A1 - Schiebel, Juliane A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Sarwar, Yasra A1 - Schierack, Peter A1 - Ali, Aamir T1 - Association of biofilm formation and cytotoxic potential with multidrug resistance in clinical isolates of Pseudomonas aeruginosa T2 - EXCLI journal Y1 - 2019 SN - 1611-2156 VL - 18 SP - 79 EP - 90 ER - TY - GEN A1 - Deutschmann, Claudia A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - The loss of tolerance to CHI3L1-A putative role in inflammatory bowel disease? T2 - Clinical Immunology Y1 - 2019 U6 - https://doi.org/10.1016/j.clim.2018.12.005 SN - 1521-6616 SN - 1521-7035 VL - 199 SP - 12 EP - 17 ER - TY - GEN A1 - Dinter, Franziska A1 - Burdukiewicz, Michał A1 - Schierack, Peter A1 - Lehmann, Werner A1 - Nestler, Jörg A1 - Dame, Gregory A1 - Rödiger, Stefan T1 - Simultaneous detection and quantification of DNA and protein biomarkers in spectrum of cardiovascular diseases in a microfluidic microbead chip T2 - Analytical and Bioanalytical Chemistry Y1 - 2019 U6 - https://doi.org/10.1007/s00216-019-02199-x SN - 1618-2650 SN - 1618-2642 VL - 411 IS - 29 SP - 7725 EP - 7735 ER - TY - GEN A1 - Deutschmann, Claudia A1 - Sowa, Mandy A1 - Murugaiyan, Jayaseelan A1 - Roessler, Uwe A1 - Röber, Nadja A1 - Conrad, Karsten A1 - Laass, Martin W. A1 - Bogdanos, Dimitrios Petrou A1 - Sipeki, Nora A1 - Papp, Maria A1 - Rödiger, Stefan A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Identification of Chitinase-3-Like Protein 1 as a Novel Neutrophil Antigenic Target in Crohn’s Disease T2 - Journal of Crohn's and Colitis N2 - Background and Aims There is an increasing incidence of inflammatory bowel disease [IBD]. Autoimmune responses are involved in the pathophysiology of IBD, but their underlying pathways and target antigens have not yet been fully elucidated. Methods Autoantigenic targets in IBD were identified after separation of whole cell proteins isolated from neutrophils using two-dimensional electrophoresis and matrix assisted laser desorption ionization – time of flight mass spectrometry-based protein identification of the spots that displayed Western blotting signals with anti-neutrophil cytoplasmic antibody-positive sera. The prevalence of IgG, IgA and secretory IgA [sIgA] to chitinase 3-like protein 1 [CHI3L1] was analysed by enzyme-linked immunosorbent assays using recombinant CHI3L1 in 110 patients with Crohn’s disease [CD], 95 with ulcerative colitis [UC], 126 with coeliac disease [CeD] and 86 healthy controls [HCs]. Results The 18-glycosylhydrolase family member CHI3L1 was identified as a neutrophil autoantigenic target. CD patients displayed significantly higher levels of IgG to CHI3L1 than patients with UC and CeD (p < 0.0001, respectively). IgA and sIgA to CHI3L1 was significantly higher in CD than in UC, CeD and HCs [p < 0.0001, respectively]. IgA and sIgA to CHI3L1 demonstrated the highest prevalence in CD [25.5%, 28/110; and 41.8%%, 46/110] compared to HCs [2.3%, 2/86; and 4.7%%, 4/86; p = 0.0015 and p < 0.0001] and are associated with a more complicated progression of CD. Conclusion CHI3L1 is a novel neutrophil autoantigenic target in CD. IgA and sIgA to CHI3L1 may serve as novel markers for CD and may facilitate the serological diagnosis of IBD. Y1 - 2019 U6 - https://doi.org/10.1093/ecco-jcc/jjz012 SN - 1876-4479 SN - 1873-9946 VL - 13 IS - 7 SP - 894 EP - 904 ER - TY - GEN A1 - Sowa, Mandy A1 - Murugaiyan, Jayaseelan A1 - Conrad, Karsten A1 - Laass, Martin W. A1 - Bogdanos, Dimitrios Petrou A1 - Papp, Maria A1 - Rödiger, Stefan A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - A novel neutrophil autoantigenic target in inflammatory bowel disease Y1 - 2018 UR - https://www.researchgate.net/publication/335889899_A_novel_neutrophil_autoantigenic_target_in_inflammatory_bowel_disease ER - TY - GEN A1 - Jurischka, Christoph A1 - Dinter, Franziska A1 - Efimova, Anastasia A1 - Weiss, Romano A1 - Schiebel, Juliane A1 - Schulz, Christian A1 - Fayziev, Bekzodjon A1 - Schierack, Peter A1 - Fischer, Thomas A1 - Rödiger, Stefan T1 - An explorative study of polymers for 3D printing of bioanalytical test systems T2 - Clinical Hemorheology and Microcirculation N2 - Background: The 3D printing is relevant as a manufacturing technology of functional models for forensic, pharmaceutical and bioanalytical applications such as drug delivery systems, sample preparation and point-of-care tests. Objective: Melting behavior and autofluorescence of materials are decisive for optimal printing and applicability of the product which are influenced by varying unknown additives. Methods: We have produced devices for bioanalytical applications from commercially available thermoplastic polymers using a melt-layer process. We characterized them by differential scanning calorimetry, fluorescence spectroscopy and functional assays (DNA capture assay, model for cell adhesion, bacterial adhesion and biofilm formation test). Results: From 14 tested colored, transparent and black materials we found only deep black acrylonitrile-butadiene-styrene (ABS) and some black polylactic acid (PLA) useable for fluorescence-based assays, with low autofluorescence only in the short-wave range of 300-400 nm. PLA was suitable for standard bioanalytical purposes due to a glass transition temperature of approximately 60°C, resistance to common laboratory chemicals and easy print processing. For temperature-critical methods, such as hybridization reactions up to 90°C, ABS was better suited. Conclusions: Autofluorescence was not a disadvantage per se but can also be used as a reference signal in assays. The rapid development of individual protocols for sample processing and analysis required the availability of a material with consistent quality over time. For fluorescence-based assays, the use of commercial standard materials did not seem to meet this requirement. KW - Thermal analysis KW - 3D Printing Y1 - 2020 UR - https://pubmed.ncbi.nlm.nih.gov/31929149/ U6 - https://doi.org/10.3233/CH-190713 SN - 1875-8622 VL - 75 IS - 1 SP - 57 EP - 84 ER -