TY - GEN A1 - Khan, Muhammad Moman A1 - Kolenda, Rafał A1 - Schierack, Peter A1 - Weinreich, Jörg A1 - Rödiger, Stefan A1 - Schierack, Jakob A1 - Stubbe, Michael A1 - Lkhagvasuren, Davaa A1 - Guenther, Sebastian A1 - Schaufler, Katharina T1 - Investigation of Commensal Escherichia coli Populations of Cormorant Hatchlings in the Absence of Anthropogenic Impacts in Remote Areas of West Mongolia T2 - Microorganisms Y1 - 2021 U6 - https://doi.org/10.3390/microorganisms9020372 SN - 2076-2607 VL - 9 IS - 2 ER - TY - GEN A1 - Reddig, Annika A1 - Lorenz, Sebastian A1 - Hiemann, Rico A1 - Guttek, Karina A1 - Hartig, Roland A1 - Heiserich, Lisa A1 - Peter, Vanessa A1 - Schierack, Peter A1 - Sack, Ulrich A1 - Roggenbuck, Dirk A1 - Reinhold, Dirk A1 - Eberle, Caroline T1 - Cytostatic drug resistance monitored by γH2AX T2 - Cytometry Part A Y1 - 2015 U6 - https://doi.org/10.1002/cyto.a.22667 SN - 1552-4930 SN - 1552-4922 VL - 87 IS - 8 SP - 724 EP - 732 ER - TY - GEN A1 - Thaler, Markus A. A1 - Bietenbeck, Andreas A1 - Steigerwald, Udo A1 - Büttner, Thomas A1 - Schierack, Peter A1 - Lindhoff-Last, Edelgard A1 - Roggenbuck, Dirk A1 - Luppa, Peter B. T1 - Evaluation of the sensitivityficity of a novel line immunoassay for the detection of criteria and non-criteria antiphospholipid antibodies in comparison to establishedELISAs T2 - PLoS One Y1 - 2019 U6 - https://doi.org/10.1371/journal.pone.0220033 SN - 1932-6203 VL - 14 IS - 7 ER - TY - GEN A1 - Kuhnert, Peter A1 - Brodard, Isabelle A1 - Ackermann, Stefanie A1 - Schierack, Peter A1 - Jores, Joerg A1 - Heliyon, J. T1 - Serological and molecular detection as well as typing of Leptospira spp. in foxes, raccoons, and other wild carnivores in North-Eastern Germany, 2021–2022 T2 - Heliyon Y1 - 2024 U6 - https://doi.org/10.1016/j.heliyon.2023.e23268 SN - 2405-8440 VL - 10 IS - 1 SP - 1 EP - 6 PB - Elsevier BV ER - TY - GEN A1 - Sowa, Mandy A1 - Großmann, Kai A1 - Knütter, Ilka A1 - Hiemann, Rico A1 - Röber, Nadja A1 - Anderer, Ursula A1 - Csernok, Elena A1 - Bogdanos, Dimitrios Petrou A1 - Borghi, Maria Orietta A1 - Meroni, Pier Luigi A1 - Schierack, Peter A1 - Reinhold, Dirk A1 - Conrad, Karsten A1 - Roggenbuck, Dirk T1 - Simultaneous automated screening and confirmatory testing for vasculitis-specific ANCA T2 - PLoS ONE KW - ANCA KW - vasculitis KW - screening KW - confirmatory testing KW - multiplexing KW - CytoBead technology Y1 - 2014 U6 - https://doi.org/10.1371/journal.pone.0107743 SN - 1932-6203 VL - 9 IS - 9 ER - TY - GEN A1 - Willitzki, Annika A1 - Hiemann, Rico A1 - Peters, Vanessa A1 - Sack, Ulrich A1 - Schierack, Peter A1 - Rödiger, Stefan A1 - Anderer, Ursula A1 - Conrad, Karsten A1 - Bogdanos, Dimitrios Petrou A1 - Reinhold, Dirk A1 - Roggenbuck, Dirk T1 - New PlatformTechnology for Comprehensive Serological Diagnostics of Autoimmune Diseases T2 - Clinical and Developmental Immunology KW - systemic autoimmune rheumatic disease (SARD) KW - automated IIF methods KW - platform for automated interpretation of cell-based IIF testing KW - microbead immunoassays KW - multiplexing Y1 - 2012 U6 - https://doi.org/10.1155/2012/284740 SN - 1740-2530 SN - 1740-2522 SP - 1 EP - 8 ER - TY - GEN A1 - Kundzer, Alena V. A1 - Volkova, Margarita V. A1 - Bogdanos, Dimitrios Petrou A1 - Rödiger, Stefan A1 - Schierack, Peter A1 - Generalov, I. A1 - Nevinsky, G. A. A1 - Roggenbuck, Dirk T1 - Deoxyribonuclease activity of polyclonal IgGs: a putative serological marker in patients with spondyloarthritides T2 - Immunologic Research N2 - Antibodies executing catalytic activity are referred to as antibody enzymes or short “abzymes” and may have diagnostic relevance. Abzymes with deoxyribonuclease (DNase) activity have been demonstrated in patients with autoimmune and infectious diseases. Despite several reports on the occurrence of DNase abzymes in systemic autoimmune rheumatic diseases, conclusive data about DNase activity of antibodies in patients with spondyloarthritides (SpAs) are lacking. In recent cross-sectional studies evaluating levels of IgG DNase activity in patients with psoriatic arthritis (PsA), reactive arthritis (ReA), and ankylosing spondylitis (AS), DNase activity of IgG has been assessed by the rivanol clot method and confirmed by agarose gel electrophoresis. Remarkably, levels of IgG DNase activity were significantly higher in sera of SpA patients than those in control subjects. In patients with PsA, ReA, and AS, a positive correlation of DNase IgG activity with synovitis, disease activity, and stage of spondylitis was observed, respectively. Given the involvement of autoimmune reactions in cytolysis and connective tissue degradation in PsA, ReA, and to a lesser extent in AS, abzymes might have an impact on the pathophysiology of SpAs. Detection of IgG DNase activity in patients suffering from SpA represents an exciting new research field and may assist in the differential diagnosis of SpA. Y1 - 2013 U6 - https://doi.org/10.1007/s12026-013-8424-9 SN - 0257-277X SN - 1559-0755 VL - 56 IS - 2-3 SP - 457 EP - 464 ER - TY - GEN A1 - Schierack, Peter A1 - Rödiger, Stefan A1 - Kuhl, Christoph A1 - Hiemann, Rico A1 - Roggenbuck, Dirk A1 - Li, Ganwu A1 - Weinreich, Jörg A1 - Berger, Enrico A1 - Nolan, Lisa K. A1 - Nicholson, Bryon A1 - Römer, Antje A1 - Frömmel, Ulrike A1 - Wieler, Lothar H. A1 - Schröder, Christian T1 - Porcine E. coli: Virulence-Associated Genes, Resistance Genes and Adhesion and Probiotic Activity Tested by a New Screening Method T2 - PLoS One N2 - We established an automated screening method to characterize adhesion of Escherichia coli to intestinal porcine epithelial cells (IPEC-J2) and their probiotic activity against infection by enteropathogenic E. coli (EPEC). 104 intestinal E. coli isolates from domestic pigs were tested by PCR for the occurrence of virulence-associated genes, genes coding for resistances to antimicrobial agents and metals, and for phylogenetic origin by PCR. Adhesion rates and probiotic activity were examined for correlation with the presence of these genes. Finally, data were compared with those from 93 E. coli isolates from wild boars. Isolates from domestic pigs carried a broad variety of all tested genes and showed great diversity in gene patterns. Adhesions varied with a maximum of 18.3 or 24.2 mean bacteria adherence per epithelial cell after 2 or 6 hours respectively. Most isolates from domestic pigs and wild boars showed low adherence, with no correlation between adhesion/probiotic activity and E. coli genes or gene clusters. The gene sfa/foc, encoding for a subunit of F1C fimbriae did show a positive correlative association with adherence and probiotic activity; however E. coli isolates from wild boars with the sfa/foc gene showed less adhesion and probiotic activity than E. coli with the sfa/foc gene isolated from domestic pigs after 6 hour incubation. In conclusion, screening porcine E. coli for virulence associated genes genes, adhesion to intestinal epithelial cells, and probiotic activity revealed a single important adhesion factor, several probiotic candidates, and showed important differences between E. coli of domestic pigs and wild boars. Y1 - 2013 U6 - https://doi.org/10.1371/journal.pone.0059242 VL - 8 IS - 4 ER - TY - GEN A1 - Roggenbuck, Dirk A1 - Reinhold, Dirk A1 - Werner, Leal A1 - Schierack, Peter A1 - Bogdanos, Dimitrios Petrou A1 - Conrad, Karsten T1 - Glycoprotein 2 antibodies in Crohn's disease. T2 - Advances in Clinical Chemistry Y1 - 2013 SN - 0065-2423 VL - 60 SP - 187 EP - 208 ER - TY - GEN A1 - Bednorz, Carmen A1 - Oelschlaeger, Kathrin A1 - Kinnemann, Bianca A1 - Hartmann, Susanne A1 - Neumann, Konrad A1 - Pieper, Robert A1 - Bethe, Astrid A1 - Semmler, Torsten A1 - Tedin, Karsten A1 - Schierack, Peter A1 - Wieler, Lothar H. A1 - Guenther, Sebastian T1 - The broader context of antibiotic resistance: zinc feed supplementation of piglets increases the proportion of multi-resistant Escherichia coli in vivo T2 - International Journal of Medical Microbiology Y1 - 2013 U6 - https://doi.org/10.1016/j.ijmm.2013.06.004 SN - 1618-0607 SN - 1438-4221 VL - 303 IS - 6-7 SP - 396 EP - 403 ER - TY - GEN A1 - Frömmel, Ulrike A1 - Lehmann, Werner A1 - Rödiger, Stefan A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Weinreich, Jörg A1 - Groß, Julia A1 - Roggenbuck, Dirk A1 - Zinke, Olaf A1 - Ansorge, Hermann A1 - Vogel, Steffen A1 - Klemm, Per A1 - Wex, Thomas A1 - Schröder, Christian A1 - Wieler, Lothar H. A1 - Schierack, Peter T1 - Adhesion of human and animal Escherichia coli strains in association with their virulence-associated genes and phylogenetic origins T2 - Applied and environmental microbiology N2 - Intestinal colonization is influenced by the ability of the bacterium to inhabit a niche, which is based on the expression of colonization factors. Escherichia coli carries a broad range of virulence-associated genes (VAGs) which contribute to intestinal (inVAGs) and extraintestinal (exVAGs) infection. Moreover, initial evidence indicates that inVAGs and exVAGs support intestinal colonization. We developed new screening tools to genotypically and phenotypically characterize E. coli isolates originating in humans, domestic pigs, and 17 wild mammal and avian species. We analyzed 317 isolates for the occurrence of 44 VAGs using a novel multiplex PCR microbead assay (MPMA) and for adhesion to four epithelial cell lines using a new adhesion assay. We correlated data for the definition of new adhesion genes. inVAGs were identified only sporadically, particularly in roe deer (Capreolus capreolus) and the European hedgehog ( Erinaceus europaeus). The prevalence of exVAGs depended on isolation from a specific host. Human uropathogenic E. coli isolates carried exVAGs with the highest prevalence, followed by badger (Meles meles) and roe deer isolates. Adhesion was found to be very diverse. Adhesion was specific to cells, host, and tissue, though it was also unspecific. Occurrence of the following VAGs was associated with a higher rate of adhesion to one or more cell lines: afa-dra, daaD, tsh, vat, ibeA, fyuA, mat, sfa-foc, malX, pic, irp2, and papC. In summary, we established new screening methods which enabled us to characterize large numbers of E. coli isolates. We defined reservoirs for potential pathogenic E. coli. We also identified a very broad range of colonization strategies and defined potential new adhesion genes. Y1 - 2013 U6 - https://doi.org/10.1128/AEM.01384-13 SN - 1098-5336 SN - 0099-2240 VL - 79 IS - 19 SP - 5814 EP - 5829 ER - TY - GEN A1 - Roggenbuck, Dirk A1 - Hiemann, Rico A1 - Schierack, Peter A1 - Reinhold, Dirk A1 - Conrad, Karsten T1 - Digital immunofluorescence enables automated detection of antinuclear antibody endpoint titers avoiding serial dilution T2 - Clinical Chemistry and Laboratory Medicine Y1 - 2013 U6 - https://doi.org/10.1515/cclm-2013-0543 SN - 1437-4331 SN - 1434-6621 VL - 52 IS - 2 SP - e9 EP - e11 ER - TY - GEN A1 - Willitzki, Annika A1 - Lorenz, Sebastian A1 - Hiemann, Rico A1 - Guttek, Karina A1 - Goihl, Alexander A1 - Hartig, Roland A1 - Conrad, Karsten A1 - Feist, Eugen A1 - Sack, Ulrich A1 - Schierack, Peter A1 - Heiserich, Lisa A1 - Eberle, Caroline A1 - Peters, Vanessa A1 - Roggenbuck, Dirk A1 - Reinhold, Dirk T1 - Fully automated analysis of chemically induced γH2AX foci in human peripheral blood mononuclear cells by indirect immunofluorescence T2 - Cytometry Part A Y1 - 2013 U6 - https://doi.org/10.1002/cyto.a.22350 SN - 1552-4930 SN - 1552-4922 VL - 83 IS - 11 SP - 1017 EP - 1026 ER - TY - GEN A1 - Bednorz, Carmen A1 - Guenther, Sebastian A1 - Oelschlaeger, Kathrin A1 - Pieper, Robert A1 - Hartmann, Susanne A1 - Tedin, Karsten A1 - Semmler, Torsten A1 - Neumann, Konrad A1 - Schierack, Peter A1 - Bethe, Astrid A1 - Wieler, Lothar H. A1 - Kinnemann, Bianca T1 - Feeding the Probiotic Enterococcus faecium Strain NCIMB 10415 to Piglets Specifically Reduces the Number of Escherichia coli Pathotypes That Adhere to the Gut Mucosa T2 - Applied and Environmental Microbiology Y1 - 2013 U6 - https://doi.org/10.1128/AEM.03138-13 SN - 0099-2240 SN - 1098-5336 VL - 79 IS - 24 SP - 7896 EP - 7904 ER - TY - GEN A1 - Frömmel, Ulrike A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Weinreich, Jörg A1 - Groß, Julia A1 - Rödiger, Stefan A1 - Wex, Thomas A1 - Ansorge, Hermann A1 - Zinke, Olaf A1 - Schröder, Christian A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Adhesion patterns of commensal and pathogenic Escherichia coli from humans and wild animals on human and porcine epithelial cell lines T2 - Gut Pathogens N2 - Abstract BACKGROUND: Different strategies of colonization or infection by E. coli result in formation of certain adhesion patterns which help also in classifying intestinal E. coli into pathotypes. Little is known about adhesion patterns and host- and tissue adaption of commensal E. coli and about E. coli originating in clinically healthy hosts carrying pathotype-specific virulence-associated genes. FINDINGS: Adhesion pattern of E. coli (n = 282) from humans and from 18 animal species were verified on intestinal human Caco-2 and porcine IPEC-J2 cells and, furthermore, for comparison on human urinary bladder 5637, porcine kidney PK-15 epithelial and HEp-2 cells. The analysis was carried out on 150,000 images of adhesion assays.Adhesion patterns were very diverse; 88 isolates were completely non-adherent, whereas 194 adhered to at least one cell line with the dominant adhesion patterns "diffusely distributed" and "microcolony formation". Adhesion patterns "chains" and "clumps" were also visible. Chain formation was mediated by the presence of epithelial cells. Clump formation was very specific on only the 5637 cell line. All enteropathogenic (eae+) E. coli (EPEC; n = 14) were able to form microcolonies which was cell line specific for each isolate. Most EPEC formed microcolonies on intestinal IPEC-J2 and Caco-2 but several also on urinary tract cells. Shigatoxin-producing (stx+) E. coli (n = 10) showed no specific adhesion patterns. CONCLUSIONS: E. coli isolates were highly diverse. Commensal and pathogenic isolates can adhere in various forms, including diffuse distribution, microcolonies, chains and clumps. Microcolony formation seems to be a global adhesion strategy also for commensal E. coli. Y1 - 2013 U6 - https://doi.org/10.1186/1757-4749-5-31 SN - 1757-4749 VL - 5 IS - 31 ER - TY - GEN A1 - Großmann, Kai A1 - Roggenbuck, Dirk A1 - Schröder, Christian A1 - Conrad, Karsten A1 - Schierack, Peter A1 - Sack, Ulrich T1 - Multiplex assessment of non-organ-specific autoantibodies with a novel microbead-based immunoassay T2 - Cytometry Part A Y1 - 2011 U6 - https://doi.org/10.1002/cyto.a.21009 SN - 1552-4922 SN - 1552-4930 VL - 79 IS - 2 SP - 118 EP - 125 ER - TY - GEN A1 - Schierack, Peter A1 - Kleta, Sylvia A1 - Tedin, Karsten A1 - Babila, T. B. A1 - Oswald, S. A1 - Oelschlaeger, T. A. A1 - Hiemann, Rico A1 - Paetzold, S. A1 - Wieler, Lothar H. T1 - E. coli Nissle 1917 affects Salmonella adhesion to porcine intestinal epithelial cells T2 - PLoS One Y1 - 2011 U6 - https://doi.org/10.1371/journal.pone.0014712 VL - 6 IS - 2 ER - TY - GEN A1 - Rödiger, Stefan A1 - Ruhland, Mirko A1 - Schröder, Christian A1 - Großmann, Kai A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Berger, Ingo A1 - Schimke, Ingolf A1 - Schierack, Peter T1 - Fluorescence dye adsorption assay to quantify carboxyl groups on the surface of poly(methyl methacrylate) microbeads T2 - Analytical Chemistry Y1 - 2011 U6 - https://doi.org/10.1021/ac103277s SN - 0003-2700 SN - 1520-6882 VL - 83 IS - 9 SP - 3379 EP - 3385 ER - TY - GEN A1 - Schierack, Peter A1 - Weinreich, Jörg A1 - Ewers, Christa A1 - Tachu, Babila A1 - Nicholson, Bryon A1 - Barth, Stefanie T1 - Hemolytic porcine intestinal E. coli without virulence-associated genes typical for intestinal pathogenic E. coli T2 - Applied and environmental microbiology Y1 - 2011 U6 - https://doi.org/10.1128/AEM.05289-11 SN - 1098-5336 SN - 0099-2240 VL - 77 IS - 23 SP - 8451 EP - 8455 ER - TY - GEN A1 - Römer, Antje A1 - Wieler, Lothar H. A1 - Schierack, Peter T1 - Analyses of intestinal commensal Escherichia coli strains from wild boars suggest adaptation to conventional pig production conditions T2 - Veterinary Microbiology Y1 - 2012 U6 - https://doi.org/10.1016/j.vetmic.2012.07.009 SN - 0378-1135 VL - 16 IS - 1-2 SP - 122 EP - 129 ER - TY - CHAP A1 - Rödiger, Stefan A1 - Schierack, Peter A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Berger, Ingo A1 - Frömmel, Ulrike A1 - Schmidt, Carsten A1 - Ruhland, Mirko A1 - Schimke, Ingolf A1 - Roggenbuck, Dirk A1 - Lehmann, Werner A1 - Schröder, Christian ED - Seitz, Harald ED - Berger, Ingo T1 - A highly versatile microscope imaging technology platform for the multiplex real-time detection of biomolecules and autoimmune antibodies T2 - Molecular diagnostics Y1 - 2013 SN - 978-3-642-37690-0 SN - 978-3-642-37691-7 SP - 35 EP - 74 PB - Springer CY - Berlin [u.a.] ER - TY - GEN A1 - Roggenbuck, Dirk A1 - Goihl, Alexander A1 - Hanack, Katja A1 - Holzlöhner, Pamela A1 - Hentschel, Christian A1 - Veiczi, Miklos A1 - Schierack, Peter A1 - Reinhold, Dirk A1 - Schulz, Hans-Ulrich T1 - Serological diagnosis and prognosis of severe acute pancreatitis by analysis of serum glycoprotein 2 T2 - Clinical Chemistry and Laboratory Medicine Y1 - 2017 U6 - https://doi.org/10.1515/cclm-2016-0797 SN - 1437-4331 SN - 1434-6621 VL - 55 IS - 6 SP - 854 EP - 864 ER - TY - GEN A1 - Durkalec, Maciej A1 - Kolenda, Rafał A1 - Owczarek, Tomasz A1 - Szkoda, Józef A1 - Nawrocka, Agnieszka A1 - Grzegrzolka, Jędrzej A1 - Dziegiel, Piotr A1 - Socha, Piotr A1 - Kolacz, Roman A1 - Schierack, Peter A1 - Zmudzki, Jan A1 - Posyniak, Andrzej T1 - Expression of metallothionein in the liver and kidneys of the red deer (Cervus elaphus L.) from an industrial metal smelting area of Poland T2 - Ecotoxicology and Environmental Safety Y1 - 2017 U6 - https://doi.org/10.1016/j.ecoenv.2016.11.018 SN - 1090-2414 SN - 0147-6513 VL - 137 SP - 121 EP - 129 ER - TY - GEN A1 - Grzymajlo, Krzysztof A1 - Ugorski, Maciej A1 - Suchanski, Jaroslaw A1 - Kedzierska, Anna E. A1 - Kolenda, Rafał A1 - Jarzab, Anna A1 - Biernatowska, Agnieszka A1 - Schierack, Peter T1 - The Novel Type 1 Fimbriae FimH Receptor Calreticulin Plays a Role in Salmonella Host Specificity T2 - Frontiers in Cellular and Infection Microbiology Y1 - 2017 U6 - https://doi.org/10.3389/fcimb.2017.00326 SN - 2235-2988 VL - 7 ER - TY - GEN A1 - Mohsin, Mashkoor A1 - Raza, Shahbaz A1 - Schaufler, Katharina A1 - Roschanski, Nicole A1 - Sarwar, Fatima A1 - Semmler, Torsten A1 - Schierack, Peter A1 - Guenther, Sebastian T1 - High Prevalence of CTX-M-15-Type ESBL-Producing E. coli from Migratory Avian Species in Pakistan T2 - Frontiers in microbiology Y1 - 2017 U6 - https://doi.org/10.3389/fmicb.2017.02476 SN - 1664-302X VL - 8 IS - 2476 ER - TY - GEN A1 - Zhang, Shulan A1 - Luo, Jing A1 - Wu, Ziyan A1 - Roggenbuck, Dirk A1 - Schierack, Peter A1 - Reinhold, Dirk A1 - Li, Ji A1 - Zeng, Xiaofeng A1 - Zhang, Fengchun A1 - Qian, Jiaming A1 - Li, Yongzhe T1 - Antibodies against glycoprotein 2 display diagnostic advantages over ASCA in distinguishing CD from intestinal tuberculosis and intestinal Behçet's disease T2 - Clinical and Translational Gastroenterology Y1 - 2018 U6 - https://doi.org/10.1038/ctg.2018.1 SN - 2155-384X VL - 9 IS - 2 SP - 133 ER - TY - GEN A1 - Schlör, Anja A1 - Holzlöhner, Pamela A1 - Listek, Martin A1 - Grieß, Cindy A1 - Butze, Monique A1 - Micheel, Burkhard A1 - Hentschel, Christian A1 - Sowa, Mandy A1 - Roggenbuck, Dirk A1 - Schierack, Peter A1 - Füner, Jonas A1 - Schliebs, Erik A1 - Goihl, Alexander A1 - Reinhold, Dirk A1 - Hanack, Katja T1 - Generation and validation of murine monoclonal and camelid recombinant single domain antibodies specific for human pancreatic glycoprotein 2 T2 - New Biotechnology Y1 - 2018 U6 - https://doi.org/10.1016/j.nbt.2018.03.006 SN - 1876-4347 SN - 1871-6784 VL - 45 SP - 60 EP - 68 ER - TY - GEN A1 - Nalli, Cecilia A1 - Somma, Valentina A1 - Andreoli, Laura A1 - Büttner, Thomas A1 - Schierack, Peter A1 - Mahler, Michael A1 - Roggenbuck, Dirk A1 - Tincani, Angela T1 - Anti-phospholipid IgG antibodies detected by line immunoassay differentiate patients with anti-phospholipid syndrome and other autoimmune diseases T2 - Autoimmunity Highlights Y1 - 2018 U6 - https://doi.org/10.1007/s13317-018-0106-0 SN - 2038-3274 SN - 2038-0305 VL - 9 IS - 1 ER - TY - GEN A1 - Ranjan, Amit A1 - Scholz, Julia A1 - Semmler, Torsten A1 - Wieler, Lothar H. A1 - Ewers, Christa A1 - Müller, Stefanie A1 - Pickard, Derek J. A1 - Schierack, Peter A1 - Tedin, Karsten A1 - Ahmed, Niyaz A1 - Schaufler, Katharina A1 - Guenther, Sebastian T1 - ESBL-plasmid carriage in E. coli enhances in vitro bacterial competition fitness and serum resistance in some strains of pandemic sequence types without overall fitness cost T2 - Gut pathogens Y1 - 2018 U6 - https://doi.org/10.1186/s13099-018-0243-z SN - 1757-4749 VL - 10 IS - 24 ER - TY - GEN A1 - Kolenda, Rafał A1 - Burdukiewicz, Michał A1 - Schiebel, Juliane A1 - Rödiger, Stefan A1 - Sauer, Lysann A1 - Szabo, Istvan A1 - Orlowska, Aleksandra A1 - Weinreich, Jörg A1 - Nitschke, Jörg A1 - Böhm, Alexander A1 - Gerber, Ulrike A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Adhesion of Salmonella to Pancreatic Secretory Granule Membrane Major Glycoprotein GP2 of Human and Porcine Origin Depends on FimH Sequence Variation T2 - Frontiers in Microbiology Y1 - 2018 U6 - https://doi.org/10.3389/fmicb.2018.01905 SN - 1664-302X VL - 9 IS - 1905 ER - TY - GEN A1 - Sowa, Mandy A1 - Kolenda, Rafał A1 - Baumgart, Daniel C. A1 - Pratschke, Johann A1 - Papp, Maria A1 - Tornai, Tamas A1 - Suchanski, Jaroslaw A1 - Bogdanos, Dimitrios Petrou A1 - Mytilinaiou, Maria G. A1 - Hammermann, Jutta A1 - Laass, Martin W. A1 - Conrad, Karsten A1 - Schramm, Christoph A1 - Franke, Andre A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Mucosal Autoimmunity to Cell-Bound GP2 Isoforms Is a Sensitive Marker in PSC and Associated With the Clinical Phenotype T2 - Frontiers in Immunology Y1 - 2018 U6 - https://doi.org/10.3389/fimmu.2018.01959 SN - 1664-3224 VL - 9 IS - 1959 ER - TY - GEN A1 - Roggenbuck, Dirk A1 - Reinhold, Dirk A1 - Schierack, Peter A1 - Bogdanos, Dimitrios Petrou A1 - Conrad, Karsten A1 - Laass, Martin W. T1 - Crohn's disease specific pancreatic antibodies: clinical and pathophysiological challenges T2 - Clinical Chemistry and Laboratory Medicine Y1 - 2013 U6 - https://doi.org/10.1515/cclm-2013-0801 SN - 1437-4331 SN - 1434-6621 VL - 52 IS - 4 SP - 494 ER - TY - GEN A1 - Olowe, Olugbenga Adekunle A1 - Choudhary, S. A1 - Schierack, Peter A1 - Wieler, Lothar H. A1 - Makanjuola, O. B. A1 - Olayemi, A. B. A1 - Anjum, M. T1 - Pathotyping bla CTX-M Escherichia coli from Nigeria T2 - European Journal of Microbiology and Immunology N2 - Background: Escherichia coli have become the enterobacteriaceae species most affected by extended-spectrum β-lactamases (ESBLs) in view of the emergence of CTX-M-type ESBLs. These CTX-M-positive E. coli have been reported in numerous regions worldwide. Virulence determinants of already reported CTX-M-positive E. coli were investigated. Methodology: To gain insights into the mechanism underlying this phenomenon, we assessed serogroup, susceptibility pattern and diversity of virulence profiles within a collection of nine blaCTX-M-positive E. coli strains and their virulent determinant using miniaturized DNA microarray techniques. The nine ESBL-positive E. coli isolates were from eight male and one female patient(s) selected for study based on previous work. Virulence potential was inferred by detection of 63 virulence factor (VF) genes. Results: Four (44.4%) of the 9 E. coli isolates exhibited the same set of core characteristics: serotype O8:Hnt, while all were positive for OXA-1, ciprofloxacin resistance. Five of the isolates exhibited highly similar (91% to 100%) VF profiles. Conclusion: The findings describe a broadly disseminated, blaCTX-M-positive and virulent E. coli serogroup with highly homogeneous virulence genotypes, suggesting recent emergence in this zone. Understanding how this clone has emerged and successfully disseminated within the hospital and community, including across national boundaries, should be a public health priority. Y1 - 2013 U6 - https://doi.org/10.1556/EuJMI.3.2013.2.5 SN - 2062-8633 VL - 3 IS - 2 SP - 120 EP - 125 ER - TY - GEN A1 - Kleta, Sylvia A1 - Nordhoff, M. A1 - Tedin, Karsten A1 - Wieler, Lothar H. A1 - Kolenda, Rafał A1 - Oswald, S. A1 - Oelschlaeger, T. A. A1 - Bleiss, W. A1 - Schierack, Peter T1 - Role of F1C fimbriae, flagella, and secreted bacterial components in the inhibitory effect of probiotic Escherichia coli Nissle 1917 on atypical enteropathogenic E. coli infection. T2 - Infect Immun. Y1 - 2014 U6 - https://doi.org/10.1128/IAI.01431-13 VL - 82 IS - 5 SP - 1801 EP - 1812 ER - TY - GEN A1 - Maslyanskiy, Aleksey A1 - Lazareva, Natalya A1 - Olinek, Polina A1 - Schierack, Peter A1 - Hentschel, Christian A1 - Cuccato, Juliane A1 - Bogdanos, Dimitrios Petrou A1 - Lapin, Sergey V. A1 - Roggenbuck, Dirk T1 - Anti-hnRNP B1 (RA33) Autoantibodies Are Associated with the Clinical Phenotype in Russian Patients with Rheumatoid Arthritis and Systemic Sclerosis T2 - Journal of Immunology Research Y1 - 2014 U6 - https://doi.org/10.1155/2014/516593 VL - 2014 ER - TY - GEN A1 - Rödiger, Stefan A1 - Liebsch, Claudia A1 - Schmidt, Carsten A1 - Lehmann, Werner A1 - Resch-Genger, U. A1 - Schedler, Uwe A1 - Schierack, Peter T1 - Nucleic acid detection based on the use of microbeads: a review T2 - Microchimica Acta Y1 - 2014 U6 - https://doi.org/10.1007/s00604-014-1243-4 SN - 0026-3672 SN - 1436-5073 VL - 181 IS - 11-12 SP - 1151 EP - 1168 ER - TY - GEN A1 - Mirsky, Vladimir M. A1 - Rödiger, Stefan A1 - Schierack, Peter A1 - Roggenbuck, Dirk T1 - Preface to the Special Issue on PCR on chip and related technologies T2 - Microchimica Acta Y1 - 2014 U6 - https://doi.org/10.1007/s00604-014-1246-1 VL - 181 IS - 13/14 SP - 1609 EP - 1610 ER - TY - GEN A1 - Sowa, Mandy A1 - Großmann, Kai A1 - Scholz, Juliane A1 - Röber, Nadja A1 - Rödiger, Stefan A1 - Schierack, Peter A1 - Conrad, Karsten A1 - Roggenbuck, Dirk A1 - Hiemann, Rico T1 - Der CytoBead-Assay – Eine neue Möglichkeit der multiparametrischen Autoantikörperanalytik bei systemischen Autoimmunerkrankungen T2 - Journal of Laboratory Medicine Y1 - 2014 U6 - https://doi.org/10.1515/labmed-2014-0041 VL - 38 IS - 6 SP - 309 EP - 317 ER - TY - GEN A1 - Roggenbuck, Dirk A1 - Somma, Valentina A1 - Schierack, Peter A1 - Borghi, Maria Orietta A1 - Meroni, Pier Luigi T1 - Autoantibody profiling in APS T2 - Lupus Y1 - 2014 U6 - https://doi.org/10.1177/0961203314534305 SN - 0961-2033 SN - 1477-0962 VL - 23 IS - 12 SP - 1262 EP - 1264 ER - TY - GEN A1 - Schierack, Peter A1 - Rödiger, Stefan A1 - Kolenda, Rafał A1 - Hiemann, Rico A1 - Berger, Enrico A1 - Grzymajlo, Krzysztof A1 - Swidsinski, Alexander A1 - Juretzek, Thomas A1 - Meissner, Dirk A1 - Mydlak, Karsten A1 - Reinhold, Dirk A1 - Nolan, Lisa K. A1 - Roggenbuck, Dirk T1 - Species-specific and pathotype-specific binding of bacteria to zymogen granule membrane glycoprotein 2 (GP2) T2 - Gut Y1 - 2015 U6 - https://doi.org/10.1136/gutjnl-2014-307854 SN - 1468-3288 SN - 0017-5749 VL - 64 IS - 3 SP - 517 EP - 519 ER - TY - GEN A1 - Mohsin, Mashkoor A1 - Guenther, Sebastian A1 - Schierack, Peter A1 - Tedin, Karsten A1 - Wieler, Lothar H. T1 - Probiotic Escherichia coli Nissle 1917 reduces growth, Shiga toxin expression, release and thus cytotoxicity of enterohemorrhagic Escherichia coli T2 - International Journal of Medical Microbiology Y1 - 2015 U6 - https://doi.org/10.1016/j.ijmm.2014.10.003 SN - 1618-0607 SN - 1438-4221 VL - 305 IS - 1 SP - 20 EP - 26 ER - TY - GEN A1 - Schierack, Peter A1 - Wieler, Lothar H. A1 - Taras, David A1 - Herwig, Volker A1 - Tachu, Babila A1 - Hlinak, Andreas A1 - Schmidt, Michael F. G. A1 - Scharek, Lydia T1 - Bacillus cereus var. toyoi enhanced systemic immune response in piglets. T2 - Veterinary immunology and immunopathology Y1 - 2007 U6 - https://doi.org/10.1016/j.vetimm.2007.03.006 SN - 1873-2534 SN - 0165-2427 VL - 118 IS - 1-2 SP - 1 EP - 11 ER - TY - GEN A1 - Schierack, Peter A1 - Walk, Nicole A1 - Reiter, Katja A1 - Weyrauch, Karl D. A1 - Wieler, Lothar H. T1 - Composition of intestinal Enterobacteriaceae populations of healthy domestic pigs. T2 - Microbiology Y1 - 2007 U6 - https://doi.org/10.1099/mic.0.2007/010173-0 SN - 1465-2080 SN - 1350-0872 VL - 153 IS - 11 SP - 3830 EP - 3837 ER - TY - GEN A1 - Schierack, Peter A1 - Walk, Nicole A1 - Ewers, Christa A1 - Wilking, Hendrik A1 - Steinrück, Hartmut A1 - Filter, Matthias A1 - Wieler, Lothar H. T1 - ExPEC-typical virulence-associated genes correlate with successful colonization by intestinal E. coli in a small piglet group. T2 - Environmental Microbiology Y1 - 2008 U6 - https://doi.org/10.1111/j.1462-2920.2008.01595.x SN - 1462-2920 SN - 1462-2912 VL - 10 IS - 7 SP - 1742 EP - 1752 ER - TY - GEN A1 - Guenther, Sebastian A1 - Schierack, Peter A1 - Grobbel, Mirjam A1 - Lübke-Becker, Antina A1 - Wieler, Lothar H. A1 - Ewers, Christa T1 - Real-time PCR assay for the detection of species of the genus Mannheimia. T2 - Journal of Microbiological Methods Y1 - 2008 U6 - https://doi.org/10.1016/j.mimet.2008.05.008 SN - 1872-8359 SN - 0167-7012 VL - 75 IS - 1 SP - 75 EP - 80 ER - TY - GEN A1 - Guenther, Sebastian A1 - Nöckler, Karsten A1 - Nickisch-Rosenegk, Markus von A1 - Landgraf, Maria A1 - Ewers, Christa A1 - Wieler, Lothar H. A1 - Schierack, Peter T1 - Detection of Trichinella spiralis, T. britovi and T. pseudospiralis in muscle tissue with real-time PCR T2 - Journal of Microbiological Methods Y1 - 2008 U6 - https://doi.org/10.1016/j.mimet.2008.06.019 SN - 0167-7012 VL - 75 IS - 2 SP - 287 EP - 292 ER - TY - GEN A1 - Guenther, Sebastian A1 - Groth, Ingrid A1 - Schierack, Peter A1 - Grabley, Susanne A1 - Munder, Thomas T1 - Direct detection of Kitasatospora species with a chaperone oligonucleotide microarray method lacking PCR amplification T2 - Journal of basic microbiology Y1 - 2008 U6 - https://doi.org/10.1002/jobm.200800038 SN - 1521-4028 SN - 0233-111X VL - 48 IS - 4 SP - 315 EP - 318 ER - TY - GEN A1 - Schierack, Peter A1 - Filter, Matthias A1 - Scharek, Lydia A1 - Toelke, Christiane A1 - Taras, David A1 - Tedin, Karsten A1 - Haverson, Karin A1 - Lübke-Becker, Antina A1 - Wieler, Lothar H. T1 - Effects of Bacillus cereus var. toyoi on immune parameters of pregnant sows T2 - Veterinary Immunology and Immunopathology Y1 - 2009 U6 - https://doi.org/10.1016/j.vetimm.2008.09.002 SN - 0165-2427 VL - 127 IS - 1-2 SP - 26 EP - 37 ER - TY - GEN A1 - Roggenbuck, Dirk A1 - Vermeire, Severine A1 - Hoffmann, Ilse A1 - Reinhold, Dirk A1 - Schierack, Peter A1 - Goihl, Alexander A1 - Arnim, Ulrike von A1 - De Hertogh, Gert A1 - Polymeros, Dimitrios A1 - Bogdanos, Dimitrios Petrou A1 - Bossuyt, Xavier T1 - Evidence of Crohn's disease-related anti-glycoprotein 2 antibodies in patients with celiac disease T2 - Clinical Chemistry and Laboratory Medicine Y1 - 2015 U6 - https://doi.org/10.1515/cclm-2014-0238 SN - 1437-4331 VL - 53 IS - 9 SP - 1349 EP - 1357 ER - TY - GEN A1 - Scholz, Juliane A1 - Großmann, Kai A1 - Knütter, Ilka A1 - Hiemann, Rico A1 - Sowa, Mandy A1 - Röber, Nadja A1 - Rödiger, Stefan A1 - Schierack, Peter A1 - Reinhold, Dirk A1 - Bogdanos, Dimitrios Petrou A1 - Meroni, Pier Luigi A1 - Radice, Antonella A1 - Conrad, Karsten A1 - Roggenbuck, Dirk T1 - Second generation analysis of antinuclear antibody (ANA) by combination of screening and confirmatory testing T2 - Clinical Chemistry and Laboratory Medicine Y1 - 2015 U6 - https://doi.org/10.1515/cclm-2015-0083 SN - 1437-4331 VL - 53 IS - 12 SP - 1991 EP - 2002 ER - TY - GEN A1 - Schmidt, Carsten A1 - Rödiger, Stefan A1 - Gruner, Melanie A1 - Moncsek, Anja A1 - Stohwasser, Ralf A1 - Hanack, Katja A1 - Schierack, Peter A1 - Schröder, Christian T1 - Multiplex localization of sequential peptide epitopes by use of a planar microbead chip T2 - Analytica Chimica Acta Y1 - 2016 U6 - https://doi.org/10.1016/j.aca.2015.12.030 SN - 0003-2670 VL - 908 SP - 150 EP - 160 ER - TY - GEN A1 - Großmann, Kai A1 - Röber, Nadja A1 - Hiemann, Rico A1 - Rödiger, Stefan A1 - Schierack, Peter A1 - Reinhold, Dirk A1 - Laass, Martin W. A1 - Conrad, Karsten A1 - Roggenbuck, Dirk T1 - Simultaneous detection of celiac disease-specific IgA antibodies and total IgA T2 - Autoimmunity Highlights Y1 - 2016 U6 - https://doi.org/10.1007/s13317-016-0073-2 SN - 2038-0305 SN - 2038-3274 VL - 7 IS - 2 ER - TY - GEN A1 - Roggenbuck, Dirk A1 - Borghi, Maria Orietta A1 - Somma, Valentina A1 - Büttner, Thomas A1 - Schierack, Peter A1 - Hanack, Katja A1 - Grossi, Claudia A1 - Bodio, Caterina A1 - Macor, Paolo A1 - Landenberg, Philipp von A1 - Boccellato, Francesco A1 - Mahler, Michael A1 - Meroni, Pier Luigi T1 - Antiphospholipid antibodies detected by line immunoassay differentiate among patients with antiphospholipid syndrome, with infections and asymptomatic carriers T2 - Arthritis Research & Therapy Y1 - 2016 U6 - https://doi.org/10.1186/s13075-016-1018-x SN - 1465-9913 SN - 1478-6362 VL - 18 IS - 111 ER - TY - GEN A1 - Burdukiewicz, Michał A1 - Rödiger, Stefan A1 - Sobczyk, Piotr A1 - Menschikowski, Mario A1 - Schierack, Peter A1 - Mackiewicz, Pawel T1 - Methods for comparing multiple digital PCR experiments T2 - Biomolecular Detection and Quantification Y1 - 2016 U6 - https://doi.org/10.1016/j.bdq.2016.06.004 SN - 2214-7535 VL - 9 SP - 14 EP - 19 ER - TY - GEN A1 - Roggenbuck, Dirk A1 - Reinhold, Dirk A1 - Baumgart, Daniel C. A1 - Schierack, Peter A1 - Conrad, Karsten A1 - Laass, Martin W. T1 - Autoimmunity in Crohn's Disease-A Putative Stratification Factor of the Clinical Phenotype T2 - Advances in Clinical Chemistry Y1 - 2016 U6 - https://doi.org/10.1016/bs.acc.2016.06.002 SN - 0065-2423 VL - 77 SP - 77 EP - 101 ER - TY - GEN A1 - Sowa, Mandy A1 - Trezzi, Barbara A1 - Hiemann, Rico A1 - Schierack, Peter A1 - Großmann, Kai A1 - Scholz, Juliane A1 - Somma, Valentina A1 - Sinico, Renato Alberto A1 - Roggenbuck, Dirk A1 - Radice, Antonella T1 - Simultaneous comprehensive multiplex autoantibody analysis for rapidly progressive glomerulonephritis T2 - Medicine Y1 - 2016 U6 - https://doi.org/10.1097/MD.0000000000005225 SN - 0025-7974 VL - 95 IS - 44 SP - 5225 ER - TY - GEN A1 - Mohsin, Mashkoor A1 - Raza, Shahbaz A1 - Roschanski, Nicole A1 - Guenther, Sebastian A1 - Ali, Aamir A1 - Schierack, Peter T1 - Description of the First Escherichia coli Clinical Isolate Harboring the Colistin Resistance Gene mcr-1 from the Indian Subcontinent T2 - Antimicrobial Agents and Chemotherapy Y1 - 2016 U6 - https://doi.org/10.1128/AAC.01945-16 SN - 1098-6596 SN - 0066-4804 VL - 61 IS - 1 ER - TY - GEN A1 - Sowa, Mandy A1 - Hiemann, Rico A1 - Schierack, Peter A1 - Reinhold, Dirk A1 - Conrad, Karsten A1 - Roggenbuck, Dirk T1 - Next-Generation Autoantibody Testing by Combination of Screening and Confirmation-the CytoBead® Technology T2 - Clinical Reviews in Allergy & Immunology Y1 - 2017 U6 - https://doi.org/10.1007/s12016-016-8574-3 SN - 1559-0267 SN - 1080-0549 VL - 53 IS - 1 SP - 87 EP - 104 ER - TY - GEN A1 - Kolenda, Rafał A1 - Burdukiewicz, Michał A1 - Schierack, Peter T1 - A systematic review and meta-analysis of the epidemiology of pathogenic Escherichia coli of calves and the role of calves as reservoirs for human pathogenic E. coli T2 - Frontiers in Cellular and Infection Microbiology Y1 - 2015 U6 - https://doi.org/10.3389/fcimb.2015.00023 SN - 2235-2988 VL - 5 ER - TY - GEN A1 - Rödiger, Stefan A1 - Kramer, Toni A1 - Frömmel, Ulrike A1 - Weinreich, Jörg A1 - Roggenbuck, Dirk A1 - Guenther, Sebastian A1 - Schaufler, Katharina A1 - Schröder, Christian A1 - Schierack, Peter T1 - Intestinal Escherichia coli colonization in a mallard duck population over four consecutive winter season T2 - Environmental Microbiology Y1 - 2015 U6 - https://doi.org/10.1111/1462-2920.12807 SN - 1462-2920 SN - 1462-2912 VL - 17 IS - 9 SP - 3352 EP - 3361 ER - TY - GEN A1 - Murugaiyan, Jayaseelan A1 - Krüger, Karolin A1 - Rösler, Uwe A1 - Weinreich, Jörg A1 - Schierack, Peter T1 - Assessment of species and antimicrobial resistance among Enterobacteriaceae isolated from mallard duck faeces T2 - Environmental Monitoring and Assessment Y1 - 2015 U6 - https://doi.org/10.1007/s10661-015-4346-4 SN - 0167-6369 SN - 1573-2959 VL - 187 IS - 3 SP - 127 EP - 137 ER - TY - GEN A1 - Lodemann, Ulrike A1 - Strahlendorf, Julia A1 - Schierack, Peter A1 - Klingspor, Shanti A1 - Aschenbach, Jörg R. A1 - Martens, Holger T1 - Effects of the Probiotic Enterococcus faecium and Pathogenic Escherichia coli Strains in a Pig and Human Epithelial Intestinal Cell Model T2 - Scientifica Y1 - 2015 U6 - https://doi.org/10.1155/2015/235184 VL - 2015 IS - 235184 ER - TY - GEN A1 - Villalta, Danilo A1 - Mytilinaiou, Maria G. A1 - Elsner, Martina A1 - Hentschel, Christian A1 - Cuccato, Juliane A1 - Somma, Valentina A1 - Schierack, Peter A1 - Roggenbuck, Dirk A1 - Bogdanos, Dimitrios Petrou T1 - Autoantibodies to asialoglycoprotein receptor (ASGPR) in patients with autoimmune liver diseases T2 - Clinica Chimica Acta Y1 - 2015 U6 - https://doi.org/10.1016/j.cca.2015.07.021 SN - 0009-8981 VL - 450 SP - 5 ER - TY - GEN A1 - Kolenda, Rafał A1 - Schierack, Peter A1 - Zieba, Filip A1 - Zwijacz-Kozica, Tomasz A1 - Bednarski, Michal T1 - First molecular characterization of Sarcocystis tenella in Tatra chamois (Rupicapra rupicapra tatrica) in Poland T2 - Parasitology Research Y1 - 2015 U6 - https://doi.org/10.1007/s00436-015-4619-4 SN - 0932-0113 SN - 1432-1955 VL - 114 IS - 10 SP - 3885 EP - 3892 ER - TY - GEN A1 - Rödiger, Stefan A1 - Burdukiewicz, Michał A1 - Blagodatskikh, Konstantin A. A1 - Jahn, Michael A1 - Schierack, Peter T1 - R as an Environment for the Reproducible Analysis of DNA Amplification Experiments T2 - The R journal Y1 - 2015 UR - https://journal.r-project.org/archive/2015/RJ-2015-011/index.html SN - 2073-4859 VL - 7 IS - 1 SP - 127 EP - 150 ER - TY - GEN A1 - Rödiger, Stefan A1 - Burdukiewicz, Michał A1 - Schierack, Peter T1 - chipPCR: an R package to pre-process raw data of amplification curves. Bioinformatics T2 - Bioinformatics Y1 - 2015 U6 - https://doi.org/10.1093/bioinformatics/btv205 SN - 1367-4803 SN - 1460-2059 VL - 31 IS - 17 SP - 2900 EP - 2902 ER - TY - CHAP A1 - Rödiger, Stefan A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Stavitskaya, Luba A1 - Gruner, Melanie A1 - Kundzer, Alena V. A1 - Volkova, Margarita V. A1 - Generalov, I. A1 - Schmidt, Carsten A1 - Schröder, Christian A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Development of a Method for Multiplex Real-Time Analysis of Enzymatic Activity on a Microbead-Chip T2 - Infection, tumors and autoimmunity, report on the 11th Dresden Symposium on Autoantibodies held in Dresden on September 1-4, 2013 Y1 - 2013 SN - 978-3-89967-881-9 SP - 275 EP - 280 PB - Pabst Science Publ. CY - Lengerich [u.a.] ER - TY - GEN A1 - Rödiger, Stefan A1 - Lehmann, Werner A1 - Frömmel, Ulrike A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Burdukiewicz, Michał A1 - Schröder, Christian A1 - Dangla, Rémi A1 - Droniou, Magali A1 - Schierack, Peter T1 - The Modi Operandi of the VideoScan Platform for the Detection and Analysis of Nucleic Acids. Life Science Day 2013 & 17. Leibniz Conference of Advanced Science, Freie Universität Berlin, 24.10.2013 Y1 - 2013 UR - https://leibniz-institut.de/Konferenzen/lsd2013/roediger_the_modi_operandi_of_the_videoscan_platform.pdf ER - TY - GEN A1 - Kramer, Toni A1 - Schiebel, Juliane A1 - Zelck, Claudia A1 - Weinreich, Jörg A1 - Schierack, Peter A1 - Rödiger, Stefan T1 - Genotyping and gene expression analysis of putative reference genes in Enterobacteriaceae, 67th Annual Meeting of the German Society of Hygiene and Microbiology (DGHM), 27.-30.09.2015, Münster Y1 - 2015 UR - https://f1000research.com/posters/5-248 U6 - https://doi.org/10.7490/f1000research.1111354.1 ER - TY - CHAP A1 - Rödiger, Stefan A1 - Burdukiewicz, Michał A1 - Heiserich, Lisa A1 - Schierack, Peter A1 - Roggenbuck, Dirk T1 - Digital Enumeration of Double Strand Breaks via γH2AX and Aassociated biomarkers in a Computing Environment for Reproducible Research T2 - From autoantibody research to standardized diagnostic assays in the management of human diseases, report on the 12th Dresden Symposium on Autoantibodies, September 23-26, 2015 Y1 - 2015 SN - 978-3-95853-104-8 SP - S. 413 PB - Pabst Science Publishers CY - Lengerich ER - TY - GEN A1 - Rödiger, Stefan A1 - Schneider, Jens A1 - Sauer, Lysann A1 - Reddig, Annika A1 - Schierack, Peter A1 - Roggenbuck, Dirk A1 - Schröder, Christian T1 - A Multiparameter Platform for The Analysis of DNA Double-strand Breaks and Associated Biomarkers after genome Editing Austrian Biomarker Symposium (ASBD) 2016, 10.-11.03.2016, Wien, Österreich Y1 - 2016 UR - https://f1000research.com/posters/5-350 ER - TY - GEN A1 - Sauer, Lysann A1 - Rödiger, Stefan A1 - Schneider, Jens A1 - Schierack, Peter A1 - Roggenbuck, Dirk A1 - Schröder, Christian T1 - Functional Multiparameter Analy sis of Double-Strand Breaks and Associate Biomarkers during Genome Editing, Genome Editing and Gene Modulation Congress 2016, 6.–8.04.2016, Oxford, United Kingdom Y1 - 2016 UR - https://f1000research.com/posters/5-706 ER - TY - CHAP A1 - Kolenda, Rafał A1 - Burdukiewicz, Michał A1 - Szabo, M. A1 - Orlowska, Aleksandra A1 - Nitschke, Jörg A1 - Böhm, Alexander A1 - Schierack, Peter T1 - Influence of Salmonella FimH sequence variation on binding to pancreatic secretory granule membrane major glycoprotein GP2 of human and porcine origin T2 - 5th ASM Conference on Salmonella, Potsdam, 29.08-01.09.2016 Y1 - 2016 UR - https://www.asm.org/images/1903%20asm%20salmonella%20program%20web3.pdf SP - S. 125 PB - American Society for Microbiology CY - Washington, DC ER - TY - CHAP A1 - Burdukiewicz, Michał A1 - Mikolajczyk, A. A1 - Szabo, Istvan A1 - Nitschke, Jörg A1 - Böhm, Alexander A1 - Schierack, Peter A1 - Kolenda, Rafał T1 - Automated enumeration of Salmonella spp. in cell line infection assays and screening of cell line infectivity among various Salmonella isolates T2 - 5th ASM Conference on Salmonella, Potsdam, 29.08-01.09.2016 Y1 - 2016 UR - https://www.asm.org/images/1903%20asm%20salmonella%20program%20web3.pdf SP - S. 127 PB - American Society for Microbiology CY - Washington, DC ER - TY - CHAP A1 - Kolenda, Rafał A1 - Burdukiewicz, Michał A1 - Schiebel, Juliane A1 - Szabo, Istvan A1 - Orlowska, Aleksandra A1 - Nitschke, Jörg A1 - Böhm, Alexander A1 - Schierack, Peter T1 - Adhesion of Salmonella to pancreatic secretory granule membrane major glycoprotein GP2 depends on FimH sequence variation T2 - Abstractbook, Microbiology and Infection, 5th Joint Conference of the DGHM & VAAM, VAAM Annual Meeting 2017, 69th Annual Meeting of the DGHM Y1 - 2017 PB - Spektrumverlag CY - Heidelberg ER - TY - GEN A1 - Deutschmann, Claudia A1 - Dinter, Franziska A1 - Rödiger, Stefan A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Comparison of Lab and Point of Care (POC) technologies – case study for CHI3L1, Potsdam Days on Bioanalysis Y1 - 2017 UR - https://www.researchgate.net/publication/321419307 ER - TY - CHAP A1 - Rödiger, Stefan A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Stavitskaya, Luba A1 - Gruner, Melanie A1 - Kundzer, Alena V. A1 - Volkova, Margarita V. A1 - Generalov, I. A1 - Schmidt, Carsten A1 - Schröder, Christian A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Development of a Method for Multiplex Real-Time Analysis of Enzymati c Activity on a Microbead-Chip. T2 - Infections, Tumors and Autoimmunity, Report on the 11th Dresden Symposium on Autoantibodies held in Dresden on September 01. - 04., 2013 Y1 - 2013 SN - 978-3-89967-881-9 SP - 280 PB - Pabst Science Publ. CY - Lengerich ER - TY - CHAP A1 - Rödiger, Stefan A1 - Hanschmann, Henning A1 - Steidle, Michael A1 - Schröder, Christian A1 - Schierack, Peter A1 - Lehmann, Werner T1 - Detection of Borrelia, Bordetella and HPV with the LoopTag Real-Time PCR Probe System T2 - International journal of medical microbiology Y1 - 2013 SN - 1618-0607 SN - 1438-4221 N1 - 65. Jahrestagung der Deutschen Gesellschaft für Hygiene und Mikrobiologie e.V. und der Jahrestagung der Deutschen Gesellschaft für Infektiologie (DGI) e.V., Universität Rostock, 22.09. – 25.09.20 13, Rostock VL - 303 IS - Supplement 1 SP - 12 EP - 13 ER - TY - GEN A1 - Dinter, Franziska A1 - Deutschmann, Claudia A1 - Schierack, Peter A1 - Dame, Gregory A1 - Rödiger, Stefan T1 - Integration of Cardiovascular Disease Biomarkers in a Microfluidic Microbead Chip Y1 - 2017 UR - https://f1000research.com/posters/6-2136 N1 - Potsdam Days on Bioanalysis 2017 ER - TY - GEN A1 - Schiebel, Juliane A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Burdukiewicz, Michał A1 - Weinreich, Jörg A1 - Ali, Aamir A1 - Roggenbuck, Dirk A1 - Rödiger, Stefan A1 - Schierack, Peter T1 - Genotypic and phenotypic characteristics in association with biofilm formation in different pathotypes of human clinical Escherichia coli isolates T2 - Applied and Environmental Microbiology Y1 - 2017 U6 - https://doi.org/10.1128/AEM.01660-17 SN - 1098-5336 SN - 0099-2240 VL - 83 IS - 24 ER - TY - GEN A1 - Liebsch, Claudia A1 - Rödiger, Stefan A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Weinreich, Jörg A1 - Fruth, Angelika A1 - Roggenbuck, Dirk A1 - Lehmann, Werner A1 - Schedler, Uwe A1 - Juretzek, Thomas A1 - Schierack, Peter T1 - Solid-phase microbead array for multiplex O-serotyping of Escherichia coli T2 - Microchimica Acta Y1 - 2017 U6 - https://doi.org/10.1007/s00604-017-2088-4 SN - 0026-3672 SN - 1436-5073 VL - 184 IS - 5 SP - 1405 EP - 1415 ER - TY - GEN A1 - Burdukiewicz, Michał A1 - Spiess, Andrej-Nikolai A1 - Blagodatskikh, Konstantin A. A1 - Lehmann, Werner A1 - Schierack, Peter A1 - Rödiger, Stefan T1 - Algorithms for automated detection of hook effect-bearing amplification curves T2 - Biomolecular Detection and Quantification Y1 - 2018 U6 - https://doi.org/10.1016/j.bdq.2018.08.001 SN - 2214-7535 VL - 16 SP - 1 EP - 4 ER - TY - GEN A1 - Deutschmann, Claudia A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - The loss of tolerance to CHI3L1-A putative role in inflammatory bowel disease? T2 - Clinical Immunology Y1 - 2018 U6 - https://doi.org/10/gftf2k SN - 1521-6616 VL - 199 SP - 12 EP - 17 ER - TY - GEN A1 - Reddig, Annika A1 - Rübe, Claudia E. A1 - Rödiger, Stefan A1 - Schierack, Peter A1 - Reinhold, Dirk A1 - Roggenbuck, Dirk T1 - DNA damage assessment and potential applications in laboratory diagnostics and precision medicine T2 - Journal of Laboratory and Precision Medicine Y1 - 2018 U6 - https://doi.org/10.21037/jlpm.2018.03.06 SN - 2519-9005 VL - 3 ER - TY - GEN A1 - Sowa, Mandy A1 - Reddig, Annika A1 - Schierack, Peter A1 - Reinhold, Dirk A1 - Roggenbuck, Dirk T1 - Phosphorylated histone 2AX foci determination in capillary blood mononuclear cells T2 - Journal of Laboratory and Precision Medicine Y1 - 2018 U6 - https://doi.org/10.21037/jlpm.2018.04.02 SN - 2519-9005 VL - 3 ER - TY - GEN A1 - Schneider, Jens A1 - Weiss, Romano A1 - Ruhe, Madeleine A1 - Jung, Tobias A1 - Roggenbuck, Dirk A1 - Stohwasser, Ralf A1 - Schierack, Peter A1 - Rödiger, Stefan T1 - Open source bioimage informatic tools for the analysis of DNA damage and associated biomarkers T2 - Journal of Laboratory and Precision Medicine KW - bioimage informatics KW - DNA damage response (DDR) KW - double-strand break (DSB) KW - immunofluorescence microscopy KW - open source software KW - proteasome activator PA28gamma Y1 - 2019 U6 - https://doi.org/10.21037/jlpm.2019.04.05 SN - 2519-9005 VL - 4 ER - TY - GEN A1 - Ruhe, Madeleine A1 - Rabe, Dominik A1 - Jurischka, Christoph A1 - Schröder, Julia A1 - Schierack, Peter A1 - Deckert, P. Markus A1 - Rödiger, Stefan T1 - Molecular biomarkers of DNA damage in diffuse large-cell lymphoma-a review T2 - Journal of Laboratory and Precision Medicine Y1 - 2019 U6 - https://doi.org/10.21037/jlpm.2019.01.01 SN - 2519-9005 VL - 4 ER - TY - GEN A1 - Umair, Muhammad A1 - Mohsin, Mashkoor A1 - Ali, Qasim A1 - Qamar, Muhammad U. A1 - Raza, Shahbaz A1 - Ali, Aamir A1 - Guenther, Sebastian A1 - Schierack, Peter T1 - Prevalence and Genetic Relatedness of Extended Spectrum-ß-Lactamase-Producing Escherichia coli Among Humans, Cattle, and Poultry in Pakistan T2 - Microbial drug resistance Y1 - 2019 U6 - https://doi.org/10.1089/mdr.2018.0450 SN - 1931-8448 SN - 1076-6294 VL - 25 IS - 9 SP - 1374 EP - 1381 ER - TY - GEN A1 - Wajid, Muhammad A1 - Awan, Asad Bashir A1 - Saleemi, Muhammad Kashif A1 - Weinreich, Jörg A1 - Schierack, Peter A1 - Sarwar, Yasra A1 - Ali, Aamir T1 - Multiple Drug Resistance and Virulence Profiling of Salmonella enterica Serovars Typhimurium and Enteritidis from Poultry Farms of Faisalabad, Pakistan T2 - Microbial Drug Resistance Y1 - 2019 U6 - https://doi.org/10.1089/mdr.2018.0121 SN - 1931-8448 SN - 1076-6294 VL - 25 IS - 1 SP - 133 EP - 142 ER - TY - GEN A1 - Akhtar, Nasrin A1 - Ghauri, M. A. A1 - Parveen, Sana A1 - Farooq, M. A1 - Ali, Aamir A1 - Schierack, Peter T1 - Comparative Analysis of Draft Genome Sequence of Rhodococcus sp. Eu-32 with Other Rhodococcus Species for Its Taxonomie Status and Sulfur Metabolism Potential T2 - Current microbiology Y1 - 2019 U6 - https://doi.org/10.1007/s00284-019-01737-1 SN - 1432-0991 SN - 0343-8651 VL - 76 IS - 10 SP - 1207 EP - 1214 ER - TY - GEN A1 - Olowe, Olugbenga Adekunle A1 - Adefioye, Olusolabomi J. A1 - Ajayeoba, T. A. A1 - Schiebel, Juliane A1 - Weinreich, Jörg A1 - Ali, Aamir A1 - Burdukiewicz, Michał A1 - Rödiger, Stefan A1 - Schierack, Peter T1 - Phylogenetic grouping and biofilm formation of Multidrug Resistant Escherichia coli Isolates from Humans, Animals and Food products in South-west Nigeria T2 - Scientific African Y1 - 2019 U6 - https://doi.org/10.1016/j.sciaf.2019.e00158 SN - 2468-2276 VL - 6 ER - TY - GEN A1 - Herrmann, Anna A1 - Rödiger, Stefan A1 - Schmidt, Carsten A1 - Schierack, Peter A1 - Schedler, Uwe T1 - Spatial Separation of Microbeads into Detection Levels by a Bioorthogonal Porous Hydrogel for Size-Selective Analysis und Increased Multiplexicity T2 - Analytical chemistry Y1 - 2019 U6 - https://doi.org/10.1021/acs.analchem.9b01586. SN - 1520-6882 SN - 0003-2700 VL - 91 IS - 13 SP - 8484 EP - 8491 ER - TY - GEN A1 - Awan, Asad Bashir A1 - Schiebel, Juliane A1 - Böhm, Alexander A1 - Nitschke, Jörg A1 - Sarwar, Yasra A1 - Schierack, Peter A1 - Ali, Aamir T1 - Association of biofilm formation and cytotoxic potential with multidrug resistance in clinical isolates of Pseudomonas aeruginosa T2 - EXCLI journal Y1 - 2019 SN - 1611-2156 VL - 18 SP - 79 EP - 90 ER - TY - GEN A1 - Deutschmann, Claudia A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - The loss of tolerance to CHI3L1-A putative role in inflammatory bowel disease? T2 - Clinical Immunology Y1 - 2019 U6 - https://doi.org/10.1016/j.clim.2018.12.005 SN - 1521-6616 SN - 1521-7035 VL - 199 SP - 12 EP - 17 ER - TY - GEN A1 - Dinter, Franziska A1 - Burdukiewicz, Michał A1 - Schierack, Peter A1 - Lehmann, Werner A1 - Nestler, Jörg A1 - Dame, Gregory A1 - Rödiger, Stefan T1 - Simultaneous detection and quantification of DNA and protein biomarkers in spectrum of cardiovascular diseases in a microfluidic microbead chip T2 - Analytical and Bioanalytical Chemistry Y1 - 2019 U6 - https://doi.org/10.1007/s00216-019-02199-x SN - 1618-2650 SN - 1618-2642 VL - 411 IS - 29 SP - 7725 EP - 7735 ER - TY - GEN A1 - Deutschmann, Claudia A1 - Sowa, Mandy A1 - Murugaiyan, Jayaseelan A1 - Roessler, Uwe A1 - Röber, Nadja A1 - Conrad, Karsten A1 - Laass, Martin W. A1 - Bogdanos, Dimitrios Petrou A1 - Sipeki, Nora A1 - Papp, Maria A1 - Rödiger, Stefan A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - Identification of Chitinase-3-Like Protein 1 as a Novel Neutrophil Antigenic Target in Crohn’s Disease T2 - Journal of Crohn's and Colitis N2 - Background and Aims There is an increasing incidence of inflammatory bowel disease [IBD]. Autoimmune responses are involved in the pathophysiology of IBD, but their underlying pathways and target antigens have not yet been fully elucidated. Methods Autoantigenic targets in IBD were identified after separation of whole cell proteins isolated from neutrophils using two-dimensional electrophoresis and matrix assisted laser desorption ionization – time of flight mass spectrometry-based protein identification of the spots that displayed Western blotting signals with anti-neutrophil cytoplasmic antibody-positive sera. The prevalence of IgG, IgA and secretory IgA [sIgA] to chitinase 3-like protein 1 [CHI3L1] was analysed by enzyme-linked immunosorbent assays using recombinant CHI3L1 in 110 patients with Crohn’s disease [CD], 95 with ulcerative colitis [UC], 126 with coeliac disease [CeD] and 86 healthy controls [HCs]. Results The 18-glycosylhydrolase family member CHI3L1 was identified as a neutrophil autoantigenic target. CD patients displayed significantly higher levels of IgG to CHI3L1 than patients with UC and CeD (p < 0.0001, respectively). IgA and sIgA to CHI3L1 was significantly higher in CD than in UC, CeD and HCs [p < 0.0001, respectively]. IgA and sIgA to CHI3L1 demonstrated the highest prevalence in CD [25.5%, 28/110; and 41.8%%, 46/110] compared to HCs [2.3%, 2/86; and 4.7%%, 4/86; p = 0.0015 and p < 0.0001] and are associated with a more complicated progression of CD. Conclusion CHI3L1 is a novel neutrophil autoantigenic target in CD. IgA and sIgA to CHI3L1 may serve as novel markers for CD and may facilitate the serological diagnosis of IBD. Y1 - 2019 U6 - https://doi.org/10.1093/ecco-jcc/jjz012 SN - 1876-4479 SN - 1873-9946 VL - 13 IS - 7 SP - 894 EP - 904 ER - TY - GEN A1 - Sowa, Mandy A1 - Murugaiyan, Jayaseelan A1 - Conrad, Karsten A1 - Laass, Martin W. A1 - Bogdanos, Dimitrios Petrou A1 - Papp, Maria A1 - Rödiger, Stefan A1 - Roggenbuck, Dirk A1 - Schierack, Peter T1 - A novel neutrophil autoantigenic target in inflammatory bowel disease Y1 - 2018 UR - https://www.researchgate.net/publication/335889899_A_novel_neutrophil_autoantigenic_target_in_inflammatory_bowel_disease ER - TY - GEN A1 - Jurischka, Christoph A1 - Dinter, Franziska A1 - Efimova, Anastasia A1 - Weiss, Romano A1 - Schiebel, Juliane A1 - Schulz, Christian A1 - Fayziev, Bekzodjon A1 - Schierack, Peter A1 - Fischer, Thomas A1 - Rödiger, Stefan T1 - An explorative study of polymers for 3D printing of bioanalytical test systems T2 - Clinical Hemorheology and Microcirculation N2 - Background: The 3D printing is relevant as a manufacturing technology of functional models for forensic, pharmaceutical and bioanalytical applications such as drug delivery systems, sample preparation and point-of-care tests. Objective: Melting behavior and autofluorescence of materials are decisive for optimal printing and applicability of the product which are influenced by varying unknown additives. Methods: We have produced devices for bioanalytical applications from commercially available thermoplastic polymers using a melt-layer process. We characterized them by differential scanning calorimetry, fluorescence spectroscopy and functional assays (DNA capture assay, model for cell adhesion, bacterial adhesion and biofilm formation test). Results: From 14 tested colored, transparent and black materials we found only deep black acrylonitrile-butadiene-styrene (ABS) and some black polylactic acid (PLA) useable for fluorescence-based assays, with low autofluorescence only in the short-wave range of 300-400 nm. PLA was suitable for standard bioanalytical purposes due to a glass transition temperature of approximately 60°C, resistance to common laboratory chemicals and easy print processing. For temperature-critical methods, such as hybridization reactions up to 90°C, ABS was better suited. Conclusions: Autofluorescence was not a disadvantage per se but can also be used as a reference signal in assays. The rapid development of individual protocols for sample processing and analysis required the availability of a material with consistent quality over time. For fluorescence-based assays, the use of commercial standard materials did not seem to meet this requirement. KW - Thermal analysis KW - 3D Printing Y1 - 2020 UR - https://pubmed.ncbi.nlm.nih.gov/31929149/ U6 - https://doi.org/10.3233/CH-190713 SN - 1875-8622 VL - 75 IS - 1 SP - 57 EP - 84 ER - TY - GEN A1 - Lopens, Steffi A1 - Krawczyk, Marcin A1 - Papp, Maria A1 - Milkiewicz, Piotr A1 - Schierack, Peter A1 - Liu, Yudong A1 - Wunsch, Ewa A1 - Conrad, Karsten A1 - Roggenbuck, Dirk T1 - The search for the Holy Grail: autoantigenic targets in primary sclerosing cholangitis asscociated with diesease phenotype an neoplasia T2 - Autoimmunity Highlights Y1 - 2020 U6 - https://doi.org/10.1186/s13317-020-00129-x SN - 2038-3274 SN - 2038-0305 VL - 11 ER - TY - GEN A1 - Schmidt, Carsten A1 - Schierack, Peter A1 - Gerber, Ulrike A1 - Schröder, Christian A1 - Choi, Youngeun A1 - Bald, Ilko A1 - Lehmann, Werner A1 - Rödiger, Stefan T1 - Streptavidin Homologues for Applications on Solid Surfaces at High Temperatures T2 - Langmuir Y1 - 2020 U6 - https://doi.org/10.1021/acs.langmuir.9b02339 SN - 1520-5827 SN - 0743-7463 VL - 36 IS - 2 SP - 628 EP - 636 ER - TY - GEN A1 - Volkov, Ilan A1 - Seguro, Luciana A1 - Leion, Elaine P. A1 - Kovács, László A1 - Roggenbuck, Dirk A1 - Schierack, Peter A1 - Gilburd, Boris A1 - Doria, Andrea A1 - Tektonidou, Maria G. A1 - Agmon-Levin, Nancy T1 - Profiles of criteria and non-criteria anti-phospholipid autoantibodies are associated with clinical phenotypes of the antiphospholipid syndrome T2 - Autoimmunity Highlights Y1 - 2020 U6 - https://doi.org/10.1186/s13317-020-00131-3 SN - 2038-3274 SN - 2038-0305 VL - 11 SP - 1 EP - 8 ER -